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The map label for this gene is hyfI [H]

Identifier: 218548077

GI number: 218548077

Start: 702733

End: 703539

Strand: Reverse

Name: hyfI [H]

Synonym: EFER_0686

Alternate gene names: 218548077

Gene position: 703539-702733 (Counterclockwise)

Preceding gene: 218548078

Following gene: 218548076

Centisome position: 15.33

GC content: 51.55

Gene sequence:

>807_bases
TTGTGTGAAAAATCTCTCTTCGGGAAATCGGGAGGCACAATGAGTTCGATGCTAAAAACCCATCGTCAACATGTTAGCCA
GCCGATGGTCCAGGATGAGCAAACGCAACAAATGAAACAGCTACTGCTCAAAGATATTCGTCGTTCAGCTTATGTCTATC
GTGTGGATTGTGGCGGTTGCAACGCCTGTGAAATTGAAATATTCGCGGCCATTACGCCTGTATTTGATGCTGAGCGCTTT
GGTATCAAAGTCGTCTCCTCACCACGCCACGCCGACATTTTGCTTTTTACTGGCGCAGTAACGCGAGCGATGCGTATGCC
TGCATTGCGGGCTTACGAATCAGCCCCCGATCATAAAATTTGTGTCTCTTACGGCGCTTGCGGTGTGGGTGGCGGAATTT
TTCACGATCTCTACTGTGTGTGGGGCGGCAGCGACACCATCGTGCCTGTTGACGTTTGGATCCCCGGTTGCCCTCCGACA
CCTGCCGCCACCATTCATGGCTTTGCTGTAGCACTGGGGTTGTTGCAACAAAAAATTCACGCGGAAGATTACGTTGAGAG
TGCGGCTGAAGCTCCGCAACTTCTGTGGCCGCAAATTCTTCCGTCTCAGCGAATTGCCATAGAGCGGGAAGCGCGGAGAC
TGGCTGGTTATCGCCAGGGGCGGGAAACCAGTGATCGCGTATTACGACTGTTAAGTGATGATCCCAGTGGGCAACAACTG
GCGCACTGGTTAGAACAAGAGAGCGATCCACGTCTTAATCAGATTGTTGATCGTCTGTTGGTGATACTTCGGGGGACTAA
TGGCTGA

Upstream 100 bases:

>100_bases
TACTCACTCAGCAACTTAACGCTGAACAAAATGAACAATTTTTGCGCCAGCAAGCCAGCATTTGCCCTGGCTGCAAACAG
CACGCTGCGCTGGTTAATGG

Downstream 100 bases:

>100_bases
TATCAGCGACGAGATCGTCTTCTGGACACTGCGACAGAAGTTTGTCGACAGCGGTACAGCAATGCCAGAGAAGAGTTCAC
AAGTGATGTACTACTCACTG

Product: hydrogenase 4, Fe-S subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MCEKSLFGKSGGTMSSMLKTHRQHVSQPMVQDEQTQQMKQLLLKDIRRSAYVYRVDCGGCNACEIEIFAAITPVFDAERF
GIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDHKICVSYGACGVGGGIFHDLYCVWGGSDTIVPVDVWIPGCPPT
PAATIHGFAVALGLLQQKIHAEDYVESAAEAPQLLWPQILPSQRIAIEREARRLAGYRQGRETSDRVLRLLSDDPSGQQL
AHWLEQESDPRLNQIVDRLLVILRGTNG

Sequences:

>Translated_268_residues
MCEKSLFGKSGGTMSSMLKTHRQHVSQPMVQDEQTQQMKQLLLKDIRRSAYVYRVDCGGCNACEIEIFAAITPVFDAERF
GIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDHKICVSYGACGVGGGIFHDLYCVWGGSDTIVPVDVWIPGCPPT
PAATIHGFAVALGLLQQKIHAEDYVESAAEAPQLLWPQILPSQRIAIEREARRLAGYRQGRETSDRVLRLLSDDPSGQQL
AHWLEQESDPRLNQIVDRLLVILRGTNG
>Mature_268_residues
MCEKSLFGKSGGTMSSMLKTHRQHVSQPMVQDEQTQQMKQLLLKDIRRSAYVYRVDCGGCNACEIEIFAAITPVFDAERF
GIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDHKICVSYGACGVGGGIFHDLYCVWGGSDTIVPVDVWIPGCPPT
PAATIHGFAVALGLLQQKIHAEDYVESAAEAPQLLWPQILPSQRIAIEREARRLAGYRQGRETSDRVLRLLSDDPSGQQL
AHWLEQESDPRLNQIVDRLLVILRGTNG

Specific function: Unknown

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=125, Percent_Identity=41.6, Blast_Score=101, Evalue=7e-22,
Organism=Escherichia coli, GI1788834, Length=245, Percent_Identity=82.8571428571429, Blast_Score=420, Evalue=1e-119,
Organism=Escherichia coli, GI1789074, Length=247, Percent_Identity=64.3724696356275, Blast_Score=323, Evalue=6e-90,
Organism=Escherichia coli, GI1788624, Length=125, Percent_Identity=40, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=123, Percent_Identity=39.8373983739837, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18859983, Length=110, Percent_Identity=40, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24642371, Length=110, Percent_Identity=40, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24651058, Length=110, Percent_Identity=40.9090909090909, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 29626; Mature: 29626

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCEKSLFGKSGGTMSSMLKTHRQHVSQPMVQDEQTQQMKQLLLKDIRRSAYVYRVDCGGC
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCC
NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDHKI
CEEEEEEEEECCCHHCHHHHCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEE
CVSYGACGVGGGIFHDLYCVWGGSDTIVPVDVWIPGCPPTPAATIHGFAVALGLLQQKIH
EEECCCCCCCCHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
AEDYVESAAEAPQLLWPQILPSQRIAIEREARRLAGYRQGRETSDRVLRLLSDDPSGQQL
HHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHH
AHWLEQESDPRLNQIVDRLLVILRGTNG
HHHHHCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MCEKSLFGKSGGTMSSMLKTHRQHVSQPMVQDEQTQQMKQLLLKDIRRSAYVYRVDCGGC
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCC
NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDHKI
CEEEEEEEEECCCHHCHHHHCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEE
CVSYGACGVGGGIFHDLYCVWGGSDTIVPVDVWIPGCPPTPAATIHGFAVALGLLQQKIH
EEECCCCCCCCHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
AEDYVESAAEAPQLLWPQILPSQRIAIEREARRLAGYRQGRETSDRVLRLLSDDPSGQQL
HHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHH
AHWLEQESDPRLNQIVDRLLVILRGTNG
HHHHHCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]