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The map label for this gene is nirA [H]

Identifier: 218532832

GI number: 218532832

Start: 5289454

End: 5291244

Strand: Reverse

Name: nirA [H]

Synonym: Mchl_4955

Alternate gene names: 218532832

Gene position: 5291244-5289454 (Counterclockwise)

Preceding gene: 218532833

Following gene: 218532831

Centisome position: 91.58

GC content: 69.57

Gene sequence:

>1791_bases
ATGGCAGACGAATTCAACGCCGAGCAGAAGCGCTACCTGGAGGGCTTCGCCTCCGGCATCGCCGCGGCCCGCATGACCGG
CGGCCTCGCCGCGGGCCAAGCCCCGGCGAGCGCACCAGCCGAGCCCACGGGCCCGGACGCGATCCACATCAAGGCGCAGA
TCCGCACCGTGAAGGAGGGCGGCAAGCTCTGCGACCAGGAGAAGTGGAAGCGCGCCGAGAACCCGTTCGACGGCCATAAT
CGGCTGAAGGCGGAGGCCGCCACGGGCAAGGCTCCGAAGCCCGAGGACAATTTCCGCTGGCGCTACCACGGCATGTTCTG
GGTCGCGCCGACCCAGACCTCCTACATGTGCCGGATCCGGGTTCCGAACGGCATCCTGAACCACTGGCAGTTCGCCGGCC
TCGCCGACCTCGCCGACGCCCATGGCGGCGCCTACAGCCACGTCACCACCCGCGCGGGCCTGCAGGTGCGCGAGATCGCG
CCCGAGCACGCCCAGCCCTTCCTCGACGGGCTGATCGACCTCGGCCTGACCGCGCAGGGCGCAGGCGCCGACAACATCCG
CAACGTCACCGGCTCGCCCGCCGCCGGCATCGACGCGCAGGAACTGCTGGACACCCGGCCGCTCACCAAGTCCTGGCACA
ACTGGATCCTGAACGACCGCTCGCTCTACGGCCTGCCGCGCAAGTTCAACGTCTCCTTCGACGGCGGCGGCGTGATGCCG
GCGCTCGAAGAGACGAACGACATCGGCTTCCAGGCGATCCAGGTGCTGGAGGGCGCGCCGGTCGCGCCGGGCGTCTGGAT
GCGCCTCGTGCTCGGCGGCATCTCGGGCCACAAGGATCTGGCCCGCGACACCGGCATCGTCCTCCGGCCGGAGGACTGCA
ACCTTGTGGCCGACGCGATCATCCGGGTCTTCATCGAGAACGGCGACCGGACCAACCGCAACAAGGCCCGGATGAAGTAC
GTCCTCGATGCCTGGGGCTTCGACAAGTACATCGCCGCGGTCGAGGAGAAGCTCGGGCGCAAGCTCGACCGGGTCGACGC
CGCCCATGTCGCCCCGCGCCGGCCGACCGACCGCTTCAGCCATGTCGGCGTGCACGCCCAGGTCCAACCCGGCCTGAACT
GGGTCGGCGTGGTGCTCCCCGTCGGCAAGATGACCACCGACCAGATGCGCGGGCTGGCAAAGATCGCCGCGGAATGCGGC
GACGGCCAGATCCGCATGACGGTCTGGCAGAACTTCATCTTCTCCGGCGTGCCGGACGCGAAGGTGGCCGAGGTCGAGGA
ACGGGTGCTCGCGCTCGGGCTCACCGTGAAGGCCGCCGGCATCCGCTCGGGCCTCGTCGCCTGCACCGGCTCGCGCGGCT
GCAAGTTCGCCGCCTCCGACACCAAGGGCCACGCGATGATCATCGCCGATCATGTCGAGCGGACCGTGCCGAACCTCGAC
GTGCCGGTGAACGTGCATCTCACCGGCTGCCACCATTCCTGCGCCCAGCACTACATCGGCGATATCGGCCTGATCGGCGC
CAAGGTCGTCGTCTCGGAGGAGGGCGACACCGTCGAGGGCTACGATGTCGTGGTCGGCGGCGGATTCGCCGAGAACCCGA
AGATCGGCACCGAGATCTGGAAGGCGGTGAAGGCCGAGGACGCGCCCTCGCGCGTCGAGGCCCTGCTCAACGCCTATCTC
GCCCGCCGCCAGGGCCCGGAGGAGAGCTTCCAGGCCTTCACCGCGCGCACCGGCCCCGAAGCCCTGCGCGACGCCGCCGA
AGAACAACCTGCCCTCAAGGCCGCCGCATGA

Upstream 100 bases:

>100_bases
AGGAGCTGATCCGCTCCGGCGCGCCGCTCTCCCCTGACGACCGGGACGACTTGATGTTCGGCCCGGCCCCGAAAACCCTC
GCAGCGTGAGTGAGGCAGCG

Downstream 100 bases:

>100_bases
CCCAGCACGTCGCGCCCCCGCTCGTTCTCATCCCCGAGAGCGCCCCCTTCAACGCCGACCAGCGCTCCTGGCTGTCCGGC
TACTTCGCCGCGCTGCTGGG

Product: ferredoxin-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MADEFNAEQKRYLEGFASGIAAARMTGGLAAGQAPASAPAEPTGPDAIHIKAQIRTVKEGGKLCDQEKWKRAENPFDGHN
RLKAEAATGKAPKPEDNFRWRYHGMFWVAPTQTSYMCRIRVPNGILNHWQFAGLADLADAHGGAYSHVTTRAGLQVREIA
PEHAQPFLDGLIDLGLTAQGAGADNIRNVTGSPAAGIDAQELLDTRPLTKSWHNWILNDRSLYGLPRKFNVSFDGGGVMP
ALEETNDIGFQAIQVLEGAPVAPGVWMRLVLGGISGHKDLARDTGIVLRPEDCNLVADAIIRVFIENGDRTNRNKARMKY
VLDAWGFDKYIAAVEEKLGRKLDRVDAAHVAPRRPTDRFSHVGVHAQVQPGLNWVGVVLPVGKMTTDQMRGLAKIAAECG
DGQIRMTVWQNFIFSGVPDAKVAEVEERVLALGLTVKAAGIRSGLVACTGSRGCKFAASDTKGHAMIIADHVERTVPNLD
VPVNVHLTGCHHSCAQHYIGDIGLIGAKVVVSEEGDTVEGYDVVVGGGFAENPKIGTEIWKAVKAEDAPSRVEALLNAYL
ARRQGPEESFQAFTARTGPEALRDAAEEQPALKAAA

Sequences:

>Translated_596_residues
MADEFNAEQKRYLEGFASGIAAARMTGGLAAGQAPASAPAEPTGPDAIHIKAQIRTVKEGGKLCDQEKWKRAENPFDGHN
RLKAEAATGKAPKPEDNFRWRYHGMFWVAPTQTSYMCRIRVPNGILNHWQFAGLADLADAHGGAYSHVTTRAGLQVREIA
PEHAQPFLDGLIDLGLTAQGAGADNIRNVTGSPAAGIDAQELLDTRPLTKSWHNWILNDRSLYGLPRKFNVSFDGGGVMP
ALEETNDIGFQAIQVLEGAPVAPGVWMRLVLGGISGHKDLARDTGIVLRPEDCNLVADAIIRVFIENGDRTNRNKARMKY
VLDAWGFDKYIAAVEEKLGRKLDRVDAAHVAPRRPTDRFSHVGVHAQVQPGLNWVGVVLPVGKMTTDQMRGLAKIAAECG
DGQIRMTVWQNFIFSGVPDAKVAEVEERVLALGLTVKAAGIRSGLVACTGSRGCKFAASDTKGHAMIIADHVERTVPNLD
VPVNVHLTGCHHSCAQHYIGDIGLIGAKVVVSEEGDTVEGYDVVVGGGFAENPKIGTEIWKAVKAEDAPSRVEALLNAYL
ARRQGPEESFQAFTARTGPEALRDAAEEQPALKAAA
>Mature_595_residues
ADEFNAEQKRYLEGFASGIAAARMTGGLAAGQAPASAPAEPTGPDAIHIKAQIRTVKEGGKLCDQEKWKRAENPFDGHNR
LKAEAATGKAPKPEDNFRWRYHGMFWVAPTQTSYMCRIRVPNGILNHWQFAGLADLADAHGGAYSHVTTRAGLQVREIAP
EHAQPFLDGLIDLGLTAQGAGADNIRNVTGSPAAGIDAQELLDTRPLTKSWHNWILNDRSLYGLPRKFNVSFDGGGVMPA
LEETNDIGFQAIQVLEGAPVAPGVWMRLVLGGISGHKDLARDTGIVLRPEDCNLVADAIIRVFIENGDRTNRNKARMKYV
LDAWGFDKYIAAVEEKLGRKLDRVDAAHVAPRRPTDRFSHVGVHAQVQPGLNWVGVVLPVGKMTTDQMRGLAKIAAECGD
GQIRMTVWQNFIFSGVPDAKVAEVEERVLALGLTVKAAGIRSGLVACTGSRGCKFAASDTKGHAMIIADHVERTVPNLDV
PVNVHLTGCHHSCAQHYIGDIGLIGAKVVVSEEGDTVEGYDVVVGGGFAENPKIGTEIWKAVKAEDAPSRVEALLNAYLA
RRQGPEESFQAFTARTGPEALRDAAEEQPALKAAA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=572, Percent_Identity=23.7762237762238, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6322597, Length=540, Percent_Identity=23.7037037037037, Blast_Score=103, Evalue=9e-23,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 64203; Mature: 64072

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEFNAEQKRYLEGFASGIAAARMTGGLAAGQAPASAPAEPTGPDAIHIKAQIRTVKEG
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEHHHC
GKLCDQEKWKRAENPFDGHNRLKAEAATGKAPKPEDNFRWRYHGMFWVAPTQTSYMCRIR
CCCCCHHHHHHCCCCCCCCHHEEEHHCCCCCCCCCCCCEEEEEEEEEEECCCCCEEEEEE
VPNGILNHWQFAGLADLADAHGGAYSHVTTRAGLQVREIAPEHAQPFLDGLIDLGLTAQG
CCCCHHHHHHHCCHHHHHHCCCCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCC
AGADNIRNVTGSPAAGIDAQELLDTRPLTKSWHNWILNDRSLYGLPRKFNVSFDGGGVMP
CCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCEECCCEEEEEEECCCCCCC
ALEETNDIGFQAIQVLEGAPVAPGVWMRLVLGGISGHKDLARDTGIVLRPEDCNLVADAI
CHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCEECCCCCCHHHHHH
IRVFIENGDRTNRNKARMKYVLDAWGFDKYIAAVEEKLGRKLDRVDAAHVAPRRPTDRFS
HHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHH
HVGVHAQVQPGLNWVGVVLPVGKMTTDQMRGLAKIAAECGDGQIRMTVWQNFIFSGVPDA
HCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCH
KVAEVEERVLALGLTVKAAGIRSGLVACTGSRGCKFAASDTKGHAMIIADHVERTVPNLD
HHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCEEEECCCCCCEEEEEHHHHHHCCCCC
VPVNVHLTGCHHSCAQHYIGDIGLIGAKVVVSEEGDTVEGYDVVVGGGFAENPKIGTEIW
CCEEEEEECCHHHHHHHHCCCEEEECEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHH
KAVKAEDAPSRVEALLNAYLARRQGPEESFQAFTARTGPEALRDAAEEQPALKAAA
HHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ADEFNAEQKRYLEGFASGIAAARMTGGLAAGQAPASAPAEPTGPDAIHIKAQIRTVKEG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEHHHC
GKLCDQEKWKRAENPFDGHNRLKAEAATGKAPKPEDNFRWRYHGMFWVAPTQTSYMCRIR
CCCCCHHHHHHCCCCCCCCHHEEEHHCCCCCCCCCCCCEEEEEEEEEEECCCCCEEEEEE
VPNGILNHWQFAGLADLADAHGGAYSHVTTRAGLQVREIAPEHAQPFLDGLIDLGLTAQG
CCCCHHHHHHHCCHHHHHHCCCCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCC
AGADNIRNVTGSPAAGIDAQELLDTRPLTKSWHNWILNDRSLYGLPRKFNVSFDGGGVMP
CCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCEECCCEEEEEEECCCCCCC
ALEETNDIGFQAIQVLEGAPVAPGVWMRLVLGGISGHKDLARDTGIVLRPEDCNLVADAI
CHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCEECCCCCCHHHHHH
IRVFIENGDRTNRNKARMKYVLDAWGFDKYIAAVEEKLGRKLDRVDAAHVAPRRPTDRFS
HHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHH
HVGVHAQVQPGLNWVGVVLPVGKMTTDQMRGLAKIAAECGDGQIRMTVWQNFIFSGVPDA
HCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCH
KVAEVEERVLALGLTVKAAGIRSGLVACTGSRGCKFAASDTKGHAMIIADHVERTVPNLD
HHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCEEEECCCCCCEEEEEHHHHHHCCCCC
VPVNVHLTGCHHSCAQHYIGDIGLIGAKVVVSEEGDTVEGYDVVVGGGFAENPKIGTEIW
CCEEEEEECCHHHHHHHHCCCEEEECEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHH
KAVKAEDAPSRVEALLNAYLARRQGPEESFQAFTARTGPEALRDAAEEQPALKAAA
HHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]