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The map label for this gene is cysJ [H]

Identifier: 218532831

GI number: 218532831

Start: 5287853

End: 5289457

Strand: Reverse

Name: cysJ [H]

Synonym: Mchl_4954

Alternate gene names: 218532831

Gene position: 5289457-5287853 (Counterclockwise)

Preceding gene: 218532832

Following gene: 218532829

Centisome position: 91.55

GC content: 68.97

Gene sequence:

>1605_bases
ATGACCCAGCACGTCGCGCCCCCGCTCGTTCTCATCCCCGAGAGCGCCCCCTTCAACGCCGACCAGCGCTCCTGGCTGTC
CGGCTACTTCGCCGCGCTGCTGGGCCCCGCCGTGGAAGGTGCCACCGCGCTCGCCCCCGGCGAGGCGCCGTCCACGGGCC
CGAAGCTCGCCGACAACGACGATGCGCCCTGGCACGACCCGTCGATGCCGATCAACGACCGCATGGCGATCGCCAAGGAC
CGCTCCGAGCCGCAGAAGCTGATGGCCGCCATGGCCCAGCAGGATTGCGGGCAGTGCGGCTATAACTGCGCCGACTACGC
CAACGCGATCTTCCTCAAGAGCGAGGAGCGGCTGAACCTTTGCCAGCCCGGCGGCAAAGAAACGCTTCGGATGCTCAAGA
AGCTCGACGAGGAGTTCGGCGCCGCGGGGGGCACGGCCGCTCCGAAGGCGGAGGCGGGCGACGCTCCGAAGAAGGAGCCG
GAACTCGGGCCGCTCGGCTATTGCCGCGAGAACCCGGTCGAGGCGACCTTCCTCTCCCGCACGCGCCTCAACGCGCCGGG
CGGCGAGAAGGAGACCTGGCATATCGAGATCGACCTGCGGGACACGCCGATCACCTACGAGGCGGGCGATTCCCTCGGCC
TGTTCCCCGCCAACGCCCCGGCCCTGGTCGATGCGGTGATCGCCGAACTCGGCACGCGGCCCGAGCGGATGATCGCGGGC
AAAACCCTGCGCGACCGGCTGCTGACCGAGTATTCCCTGGGTGCCGCGCCGGACAACCTCTACCAGCTCCTCTCGCTCCT
GACCTCGGGTGCCGCCCGGAAGAAGGCGCAGGCGCTCGCAGCCGGCGAGGATCCGGACGGCGATGCGCAGCATCTCGACG
TGCTCGGCGCGCTCCACAAATTCCCCGGTGCGCGCCCCGACGCCGAGGTCTTCCTCGAGGCGCTCGACGAGTTGCAGCCG
CGGCTCTACTCGATCTCCTCCTCGCCGAAGATGGATGAGGGCCGGGTTTCGCTCACCGTCGATGCGGTGCGCTACAACCA
TCGCTCGCGCCTGCGCCTCGGCGTCGCCTCCACGCATCTGGGCGAGCGTCTCCCGGAGCAGACGAAGGTCAGGATCTACC
TGCAGAAGGCGCACGGCTTCGGCCTGCCGGCGGATGCAGCCAAGGACGTGATCATGTGCGGCCCCGGCACCGGCGTCGCC
CCGTTCCGCTCCTTCCTGCGTGAGCGCGCGGCCACGAAGGCCCCCGGCCGCAACTGGCTATTCTACGGCCACCAGCGGCA
GGCGACCGACTTCTTCTACAAGGACGAGCTGAACGGCCTGAAAGAAGACAAGGTGCTGACGCGCCTCTCGCTGGCTTGGT
CGCGCGACGGCACGGAAAAGACCTACGTGCAGGACCGCATGCGCGAGAACGGCGCCGAGCTGTGGAAATGGCTGGAGGGC
GGCGCCCATTTCTACGTCTGCGGCGATGCCAAGCGCATGGCCAAGGACGTGGAGCGGGCGATCATCGACGTCGCCGCCCA
GTTCGGCGGCAAGAACCCCGACGAGGCCGTGGCCTACCTCGCCGCCCTCAAGAAGAGCGGGCGCTATCAGGCCGACGTGT
ACTGA

Upstream 100 bases:

>100_bases
GCCAGGGCCCGGAGGAGAGCTTCCAGGCCTTCACCGCGCGCACCGGCCCCGAAGCCCTGCGCGACGCCGCCGAAGAACAA
CCTGCCCTCAAGGCCGCCGC

Downstream 100 bases:

>100_bases
GGGGGAGGGGGTTTTCCCCTCCCACCGCCTCAGGCCGCCGGGTTGACCCGCGGATCGGCGCGCAGCCGTGCCGCGGCGAG
ATCGACGAAGGCGCGAGTCT

Product: sulfite reductase

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MTQHVAPPLVLIPESAPFNADQRSWLSGYFAALLGPAVEGATALAPGEAPSTGPKLADNDDAPWHDPSMPINDRMAIAKD
RSEPQKLMAAMAQQDCGQCGYNCADYANAIFLKSEERLNLCQPGGKETLRMLKKLDEEFGAAGGTAAPKAEAGDAPKKEP
ELGPLGYCRENPVEATFLSRTRLNAPGGEKETWHIEIDLRDTPITYEAGDSLGLFPANAPALVDAVIAELGTRPERMIAG
KTLRDRLLTEYSLGAAPDNLYQLLSLLTSGAARKKAQALAAGEDPDGDAQHLDVLGALHKFPGARPDAEVFLEALDELQP
RLYSISSSPKMDEGRVSLTVDAVRYNHRSRLRLGVASTHLGERLPEQTKVRIYLQKAHGFGLPADAAKDVIMCGPGTGVA
PFRSFLRERAATKAPGRNWLFYGHQRQATDFFYKDELNGLKEDKVLTRLSLAWSRDGTEKTYVQDRMRENGAELWKWLEG
GAHFYVCGDAKRMAKDVERAIIDVAAQFGGKNPDEAVAYLAALKKSGRYQADVY

Sequences:

>Translated_534_residues
MTQHVAPPLVLIPESAPFNADQRSWLSGYFAALLGPAVEGATALAPGEAPSTGPKLADNDDAPWHDPSMPINDRMAIAKD
RSEPQKLMAAMAQQDCGQCGYNCADYANAIFLKSEERLNLCQPGGKETLRMLKKLDEEFGAAGGTAAPKAEAGDAPKKEP
ELGPLGYCRENPVEATFLSRTRLNAPGGEKETWHIEIDLRDTPITYEAGDSLGLFPANAPALVDAVIAELGTRPERMIAG
KTLRDRLLTEYSLGAAPDNLYQLLSLLTSGAARKKAQALAAGEDPDGDAQHLDVLGALHKFPGARPDAEVFLEALDELQP
RLYSISSSPKMDEGRVSLTVDAVRYNHRSRLRLGVASTHLGERLPEQTKVRIYLQKAHGFGLPADAAKDVIMCGPGTGVA
PFRSFLRERAATKAPGRNWLFYGHQRQATDFFYKDELNGLKEDKVLTRLSLAWSRDGTEKTYVQDRMRENGAELWKWLEG
GAHFYVCGDAKRMAKDVERAIIDVAAQFGGKNPDEAVAYLAALKKSGRYQADVY
>Mature_533_residues
TQHVAPPLVLIPESAPFNADQRSWLSGYFAALLGPAVEGATALAPGEAPSTGPKLADNDDAPWHDPSMPINDRMAIAKDR
SEPQKLMAAMAQQDCGQCGYNCADYANAIFLKSEERLNLCQPGGKETLRMLKKLDEEFGAAGGTAAPKAEAGDAPKKEPE
LGPLGYCRENPVEATFLSRTRLNAPGGEKETWHIEIDLRDTPITYEAGDSLGLFPANAPALVDAVIAELGTRPERMIAGK
TLRDRLLTEYSLGAAPDNLYQLLSLLTSGAARKKAQALAAGEDPDGDAQHLDVLGALHKFPGARPDAEVFLEALDELQPR
LYSISSSPKMDEGRVSLTVDAVRYNHRSRLRLGVASTHLGERLPEQTKVRIYLQKAHGFGLPADAAKDVIMCGPGTGVAP
FRSFLRERAATKAPGRNWLFYGHQRQATDFFYKDELNGLKEDKVLTRLSLAWSRDGTEKTYVQDRMRENGAELWKWLEGG
AHFYVCGDAKRMAKDVERAIIDVAAQFGGKNPDEAVAYLAALKKSGRYQADVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=400, Percent_Identity=32.75, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI7657393, Length=392, Percent_Identity=29.0816326530612, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI24041029, Length=396, Percent_Identity=31.8181818181818, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI221316709, Length=392, Percent_Identity=28.8265306122449, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI221316705, Length=401, Percent_Identity=28.428927680798, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI169790956, Length=427, Percent_Identity=27.4004683840749, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI169790958, Length=427, Percent_Identity=27.4004683840749, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI10835173, Length=403, Percent_Identity=30.5210918114144, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI40254422, Length=411, Percent_Identity=29.9270072992701, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI221316707, Length=443, Percent_Identity=25.9593679458239, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1789123, Length=397, Percent_Identity=40.3022670025189, Blast_Score=290, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17554134, Length=449, Percent_Identity=33.184855233853, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17566446, Length=374, Percent_Identity=27.0053475935829, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17531441, Length=259, Percent_Identity=30.1158301158301, Blast_Score=86, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6321832, Length=427, Percent_Identity=28.5714285714286, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321143, Length=382, Percent_Identity=25.9162303664921, Blast_Score=116, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6325305, Length=375, Percent_Identity=24.8, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137192, Length=395, Percent_Identity=30.126582278481, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24582192, Length=395, Percent_Identity=30.126582278481, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI78706872, Length=375, Percent_Identity=32.2666666666667, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24583543, Length=375, Percent_Identity=32.2666666666667, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI78706876, Length=375, Percent_Identity=32.2666666666667, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24660907, Length=230, Percent_Identity=29.1304347826087, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24660903, Length=230, Percent_Identity=29.1304347826087, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647438, Length=400, Percent_Identity=23.75, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 58221; Mature: 58090

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQHVAPPLVLIPESAPFNADQRSWLSGYFAALLGPAVEGATALAPGEAPSTGPKLADND
CCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCC
DAPWHDPSMPINDRMAIAKDRSEPQKLMAAMAQQDCGQCGYNCADYANAIFLKSEERLNL
CCCCCCCCCCCCCCEEHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCEEEEECCCCCCC
CQPGGKETLRMLKKLDEEFGAAGGTAAPKAEAGDAPKKEPELGPLGYCRENPVEATFLSR
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
TRLNAPGGEKETWHIEIDLRDTPITYEAGDSLGLFPANAPALVDAVIAELGTRPERMIAG
HCCCCCCCCCEEEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHH
KTLRDRLLTEYSLGAAPDNLYQLLSLLTSGAARKKAQALAAGEDPDGDAQHLDVLGALHK
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
FPGARPDAEVFLEALDELQPRLYSISSSPKMDEGRVSLTVDAVRYNHRSRLRLGVASTHL
CCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEHHHHCCCHHEEEEHHHHHH
GERLPEQTKVRIYLQKAHGFGLPADAAKDVIMCGPGTGVAPFRSFLRERAATKAPGRNWL
HHHCCCCCEEEEEEEECCCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCEE
FYGHQRQATDFFYKDELNGLKEDKVLTRLSLAWSRDGTEKTYVQDRMRENGAELWKWLEG
EECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
GAHFYVCGDAKRMAKDVERAIIDVAAQFGGKNPDEAVAYLAALKKSGRYQADVY
CCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCCC
>Mature Secondary Structure 
TQHVAPPLVLIPESAPFNADQRSWLSGYFAALLGPAVEGATALAPGEAPSTGPKLADND
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCC
DAPWHDPSMPINDRMAIAKDRSEPQKLMAAMAQQDCGQCGYNCADYANAIFLKSEERLNL
CCCCCCCCCCCCCCEEHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCEEEEECCCCCCC
CQPGGKETLRMLKKLDEEFGAAGGTAAPKAEAGDAPKKEPELGPLGYCRENPVEATFLSR
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
TRLNAPGGEKETWHIEIDLRDTPITYEAGDSLGLFPANAPALVDAVIAELGTRPERMIAG
HCCCCCCCCCEEEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHH
KTLRDRLLTEYSLGAAPDNLYQLLSLLTSGAARKKAQALAAGEDPDGDAQHLDVLGALHK
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
FPGARPDAEVFLEALDELQPRLYSISSSPKMDEGRVSLTVDAVRYNHRSRLRLGVASTHL
CCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEHHHHCCCHHEEEEHHHHHH
GERLPEQTKVRIYLQKAHGFGLPADAAKDVIMCGPGTGVAPFRSFLRERAATKAPGRNWL
HHHCCCCCEEEEEEEECCCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCEE
FYGHQRQATDFFYKDELNGLKEDKVLTRLSLAWSRDGTEKTYVQDRMRENGAELWKWLEG
EECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
GAHFYVCGDAKRMAKDVERAIIDVAAQFGGKNPDEAVAYLAALKKSGRYQADVY
CCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA