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The map label for this gene is nasD [H]

Identifier: 218532833

GI number: 218532833

Start: 5291256

End: 5292476

Strand: Reverse

Name: nasD [H]

Synonym: Mchl_4956

Alternate gene names: 218532833

Gene position: 5292476-5291256 (Counterclockwise)

Preceding gene: 218532834

Following gene: 218532832

Centisome position: 91.6

GC content: 71.58

Gene sequence:

>1221_bases
ATGAGCGTGAAGGACAGACTCGTCGTTGTCGGCAACGGCATGGCCTCGCTCCGCTTCCTCGAGCGGCTGACGGAGCGCGC
GCCGGGCCGCTACGACGTGACGGTGGTCGGCGCCGAGCCGGTGGCGGCCTATAACCGGGTGCTGCTCTCCTCGCTGCTCG
GCGGCGAGGTGGACGAGGCGGCCTGCGGCTTCCGTCCGCTCTCCTGGTACGAGGAGCACGGCATCCGCCTCGTCACCGGC
GCACCGGTGACGGAGATCGACCGGGCGAACCGCATCGCGCTGGTCGGCTCCGCCCATGTGCTGCCCTACGACAAGCTGGT
GCTGGCGGTGGGCTCACTGCCGATCCGGCTGCCGCTGCCCGGCATCGACCTGCCCGGCGTCCTCACCTTCCGCGATTTCG
CCGATGTGGCGGCGATCCGCAAAGCCGCCGTCGAGCATGCCAAGGCGGTGGTGATCGGCGGCGGACTTCTGGGCCTGGAG
GCCGCGGTCGGGCTCGCCCGGCTCGGGGTCGACACCACGCTGATCCACGTCATGGACCGGGTGATGGAGCGCCAGCTCGA
CCACACGGCCGCCCGCCTCGTGCGCACGGCGATGGAAGCGCGCGGCGTCAAGGTGATGCTGTCCGCCGACACCGCCGCGA
TCGAGGGCGACGGACGCGTCGAGCGCCTGCGGATGAAGGACGGCACCGTGATCCCGGCCGACCTCGTGGTGATGTCGGTC
GGCATCCGCGCCTCGACCGCCCTGGCGCAGTCCTGCGGGCTCGCCTGCGGGCGCGGCATCACGGTGGACGACCGGATGAC
CACCTCCGATCCGGCGATCCATGCGCTCGGCGAATGCGCCGAGCACAGGGGCGTCGTTTACGGCCTCGTCGAGCCCGCCT
ACGAGCAGGCCGAGGTGCTGTCGCGGGCGCTTCTCGGCGAGGCGGCGGCCTATCCCGGCACGTCCCTTGCCACCAGCCTC
AAGGTGTCGGGCCTGCCAGTCTTCTCGGCCGGATTGGTCGATACGCCCGAGGGTGCCGAGGCGGTCGTGATGCACGACGC
CGCCGCCGGCCTCTACCGCAAGCTGATCATCGCCGACGGCCGCCTCGCGGGGGCGGTCTTCGTCGGCGATATCGCGGAGC
AGGCGCGCTGCAAGGAGCTGATCCGCTCCGGCGCGCCGCTCTCCCCTGACGACCGGGACGACTTGATGTTCGGCCCGGCC
CCGAAAACCCTCGCAGCGTGA

Upstream 100 bases:

>100_bases
ACCTACGTCCACGCCCGCGCCGCGGTGCTGGAATTCCTCTACGCCCGCCACGCCAAGCCTGCTCTCGCTGCCTGAGTTCG
TGAAGGGGGATCACGGGTTC

Downstream 100 bases:

>100_bases
GTGAGGCAGCGATGGCAGACGAATTCAACGCCGAGCAGAAGCGCTACCTGGAGGGCTTCGCCTCCGGCATCGCCGCGGCC
CGCATGACCGGCGGCCTCGC

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSVKDRLVVVGNGMASLRFLERLTERAPGRYDVTVVGAEPVAAYNRVLLSSLLGGEVDEAACGFRPLSWYEEHGIRLVTG
APVTEIDRANRIALVGSAHVLPYDKLVLAVGSLPIRLPLPGIDLPGVLTFRDFADVAAIRKAAVEHAKAVVIGGGLLGLE
AAVGLARLGVDTTLIHVMDRVMERQLDHTAARLVRTAMEARGVKVMLSADTAAIEGDGRVERLRMKDGTVIPADLVVMSV
GIRASTALAQSCGLACGRGITVDDRMTTSDPAIHALGECAEHRGVVYGLVEPAYEQAEVLSRALLGEAAAYPGTSLATSL
KVSGLPVFSAGLVDTPEGAEAVVMHDAAAGLYRKLIIADGRLAGAVFVGDIAEQARCKELIRSGAPLSPDDRDDLMFGPA
PKTLAA

Sequences:

>Translated_406_residues
MSVKDRLVVVGNGMASLRFLERLTERAPGRYDVTVVGAEPVAAYNRVLLSSLLGGEVDEAACGFRPLSWYEEHGIRLVTG
APVTEIDRANRIALVGSAHVLPYDKLVLAVGSLPIRLPLPGIDLPGVLTFRDFADVAAIRKAAVEHAKAVVIGGGLLGLE
AAVGLARLGVDTTLIHVMDRVMERQLDHTAARLVRTAMEARGVKVMLSADTAAIEGDGRVERLRMKDGTVIPADLVVMSV
GIRASTALAQSCGLACGRGITVDDRMTTSDPAIHALGECAEHRGVVYGLVEPAYEQAEVLSRALLGEAAAYPGTSLATSL
KVSGLPVFSAGLVDTPEGAEAVVMHDAAAGLYRKLIIADGRLAGAVFVGDIAEQARCKELIRSGAPLSPDDRDDLMFGPA
PKTLAA
>Mature_405_residues
SVKDRLVVVGNGMASLRFLERLTERAPGRYDVTVVGAEPVAAYNRVLLSSLLGGEVDEAACGFRPLSWYEEHGIRLVTGA
PVTEIDRANRIALVGSAHVLPYDKLVLAVGSLPIRLPLPGIDLPGVLTFRDFADVAAIRKAAVEHAKAVVIGGGLLGLEA
AVGLARLGVDTTLIHVMDRVMERQLDHTAARLVRTAMEARGVKVMLSADTAAIEGDGRVERLRMKDGTVIPADLVVMSVG
IRASTALAQSCGLACGRGITVDDRMTTSDPAIHALGECAEHRGVVYGLVEPAYEQAEVLSRALLGEAAAYPGTSLATSLK
VSGLPVFSAGLVDTPEGAEAVVMHDAAAGLYRKLIIADGRLAGAVFVGDIAEQARCKELIRSGAPLSPDDRDDLMFGPAP
KTLAA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=319, Percent_Identity=25.705329153605, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI226437568, Length=319, Percent_Identity=25.705329153605, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI21389617, Length=319, Percent_Identity=25.705329153605, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI4757732, Length=215, Percent_Identity=26.046511627907, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI22202629, Length=215, Percent_Identity=26.046511627907, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789765, Length=405, Percent_Identity=36.2962962962963, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1789065, Length=326, Percent_Identity=26.6871165644172, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788892, Length=219, Percent_Identity=29.6803652968037, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17559934, Length=287, Percent_Identity=26.4808362369338, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24639257, Length=379, Percent_Identity=25.3298153034301, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281359715, Length=379, Percent_Identity=25.3298153034301, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI281359713, Length=379, Percent_Identity=25.3298153034301, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24639250, Length=379, Percent_Identity=25.3298153034301, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI18543267, Length=379, Percent_Identity=25.3298153034301, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24639252, Length=379, Percent_Identity=25.3298153034301, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585130, Length=280, Percent_Identity=23.5714285714286, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI28573993, Length=196, Percent_Identity=27.0408163265306, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24581020, Length=196, Percent_Identity=27.0408163265306, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 42592; Mature: 42461

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKDRLVVVGNGMASLRFLERLTERAPGRYDVTVVGAEPVAAYNRVLLSSLLGGEVDEA
CCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHH
ACGFRPLSWYEEHGIRLVTGAPVTEIDRANRIALVGSAHVLPYDKLVLAVGSLPIRLPLP
HCCCCCCCHHHHCCEEEEECCCHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCEEECCC
GIDLPGVLTFRDFADVAAIRKAAVEHAKAVVIGGGLLGLEAAVGLARLGVDTTLIHVMDR
CCCCCCEEEHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHCHHHHHHHHHHH
VMERQLDHTAARLVRTAMEARGVKVMLSADTAAIEGDGRVERLRMKDGTVIPADLVVMSV
HHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCCCEEEEEECCCCCCCHHHHHHCC
GIRASTALAQSCGLACGRGITVDDRMTTSDPAIHALGECAEHRGVVYGLVEPAYEQAEVL
CCHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHH
SRALLGEAAAYPGTSLATSLKVSGLPVFSAGLVDTPEGAEAVVMHDAAAGLYRKLIIADG
HHHHHHHHHCCCCCHHHHEEEECCCCEEECCCCCCCCCCCEEEEEHHHHHHHHHHHEECC
RLAGAVFVGDIAEQARCKELIRSGAPLSPDDRDDLMFGPAPKTLAA
CEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCHHHCC
>Mature Secondary Structure 
SVKDRLVVVGNGMASLRFLERLTERAPGRYDVTVVGAEPVAAYNRVLLSSLLGGEVDEA
CCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHH
ACGFRPLSWYEEHGIRLVTGAPVTEIDRANRIALVGSAHVLPYDKLVLAVGSLPIRLPLP
HCCCCCCCHHHHCCEEEEECCCHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCEEECCC
GIDLPGVLTFRDFADVAAIRKAAVEHAKAVVIGGGLLGLEAAVGLARLGVDTTLIHVMDR
CCCCCCEEEHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHCHHHHHHHHHHH
VMERQLDHTAARLVRTAMEARGVKVMLSADTAAIEGDGRVERLRMKDGTVIPADLVVMSV
HHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCCCEEEEEECCCCCCCHHHHHHCC
GIRASTALAQSCGLACGRGITVDDRMTTSDPAIHALGECAEHRGVVYGLVEPAYEQAEVL
CCHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHH
SRALLGEAAAYPGTSLATSLKVSGLPVFSAGLVDTPEGAEAVVMHDAAAGLYRKLIIADG
HHHHHHHHHCCCCCHHHHEEEECCCCEEECCCCCCCCCCCEEEEEHHHHHHHHHHHEECC
RLAGAVFVGDIAEQARCKELIRSGAPLSPDDRDDLMFGPAPKTLAA
CEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]