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The map label for this gene is lldP1 [H]

Identifier: 218232487

GI number: 218232487

Start: 634734

End: 636353

Strand: Reverse

Name: lldP1 [H]

Synonym: BCB4264_A0648

Alternate gene names: 218232487

Gene position: 636353-634734 (Counterclockwise)

Preceding gene: 218234132

Following gene: 218234991

Centisome position: 11.74

GC content: 35.31

Gene sequence:

>1620_bases
ATGAGTACTTGGACACAAATTTATGACCCATTAAACAATATTTGGTTATCTGCACTAATCGCAGCACTTCCAATCTTTTG
TTTCATCATTTGTTTAATGGGATTCAAAATGAAAAGTTACATGGCTGGTCTTTATAGTGTGATTGTCGCTATTATCCTTG
CAATATTTGTTTATAAAATGCCAGCAACAGTAGCTGTAGCATCTGCCGGATTCGGTGTTTTATCTGGATTTTATCCAATT
TGTACTATCGTTATCGCCGCAATTTTCCTTTACAAATTAACTGTTAAAACAGAGCAATTCAATATTATTCGCGATAGTAT
TTCAAGCATTACAAATGATCAACGTTTACAAGTATTATTAATCGCTTATTCTTTCGGTGCTTTCTTAGAAGGTGCAGCTG
GTCTAGGGGTTCCAGTTGCTATTACAGCCGCATTACTTGTAGGTATGGGCTTTAATCCATTAAAAGCAGCAGGTATTTGT
TTAGTAGCTAATATTGCTGGTGGTGCTATGGGTGCTATGGGTATTCCAGTTACAGTGCCAGCACAATTAACTCAACTTGA
CGCATTAACTATTGGTCGTCAAACTGTTCTTATTTTACCTTTCATTAGTATTATTTTACCTTTCTTACTTGTTTCAATGG
TCGATGGAATTAAAGGTATTAAAGAAACTTGGCAAGGTATTCTTGTAAGTGGTGTTTCTTTCGCTATTACTCAATTCGCT
GTGACTTACTTCTTAGGTGCAGAACTTACTAATATTTTCGCAGCAGTTGTAAGTATGATTGCTCTTGCATTATTCTTACG
TGTTTGGCAACCAAAATCTTCTACTAAAGAAGAAAAACAAGAAACTAAAGTATCACATACATTCAATCAAATTTTATACG
CTTGGTCTCCATTCGTATTCTTAACTGCATTTGTAACAATCTTTAACTTAAAACCGATTAAAGCTTTATTTGCTCCAGAT
GGTGCGTTAGCAAACTTAGTATTTAACATTCAGTTCCCTGGTCTTCATAATGCTGTTATGAAAACTACACCAATTACAAA
AGCTGATACACCTTTCGCAGCTGTTTTCAAATTTGATGTATTCTCTTCTACAACTACTGCGATTGTACTAGCAATTATCG
TTTCACTTATCGTTTACCGCGTAAAAGGTAAAATGGTTAAAGAATTAATGATTGAAACAATGAAAGAATTAAAAGCTCCA
GTATATACAATTTGTAGCGTTATCGCTTTAGCTTACGTAGAAAACTACTCTGGTATGTCATCTACACTTGGACTTGCATT
CTCTTCTACTGGTAATGCATTCCCAGTTCTTTCTCCAATCTTAGGATGGATTGGCGTATTCATTACAGGTTCAGTAGTTT
CAAGTGGTTCTTTATTCGCACCACTTCAAGCAGTAACAGCAGACCAGTTAAACATCGTTCCTTCTACACTCGTTGCCTTA
AACGTAGTAGGTGGTACAATGGCAAAAATGGTTTCACCACAATCTGTAGCAGTTGCTTGTGCCGCAGTTGGACTAGTTGG
TAAAGAATCTGCTCTATTTAAAACAGCAATGAAATACAGTTTAATCTTCTTAGCTGTTATTAGCTTACTTCAATTAAACG
CTGTATTTAATATCTTTTAA

Upstream 100 bases:

>100_bases
AGTGTTGAAGGAGAATATACTAAGCATGTAAGTTGATTGTGAATAGTTTCACAAATAATGTGAATTTCTTCACATATAAT
AGATAGGAGTTGTCATTATT

Downstream 100 bases:

>100_bases
AAATAAAACACCGATCCAAGTGGATCGGTGTTTTTCACATTACATTTTTTTATTTTTTAATAAAGAAGCTCCAGCAATTT
CAGGGTGAGTCATTTCGAAT

Product: L-lactate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MSTWTQIYDPLNNIWLSALIAALPIFCFIICLMGFKMKSYMAGLYSVIVAIILAIFVYKMPATVAVASAGFGVLSGFYPI
CTIVIAAIFLYKLTVKTEQFNIIRDSISSITNDQRLQVLLIAYSFGAFLEGAAGLGVPVAITAALLVGMGFNPLKAAGIC
LVANIAGGAMGAMGIPVTVPAQLTQLDALTIGRQTVLILPFISIILPFLLVSMVDGIKGIKETWQGILVSGVSFAITQFA
VTYFLGAELTNIFAAVVSMIALALFLRVWQPKSSTKEEKQETKVSHTFNQILYAWSPFVFLTAFVTIFNLKPIKALFAPD
GALANLVFNIQFPGLHNAVMKTTPITKADTPFAAVFKFDVFSSTTTAIVLAIIVSLIVYRVKGKMVKELMIETMKELKAP
VYTICSVIALAYVENYSGMSSTLGLAFSSTGNAFPVLSPILGWIGVFITGSVVSSGSLFAPLQAVTADQLNIVPSTLVAL
NVVGGTMAKMVSPQSVAVACAAVGLVGKESALFKTAMKYSLIFLAVISLLQLNAVFNIF

Sequences:

>Translated_539_residues
MSTWTQIYDPLNNIWLSALIAALPIFCFIICLMGFKMKSYMAGLYSVIVAIILAIFVYKMPATVAVASAGFGVLSGFYPI
CTIVIAAIFLYKLTVKTEQFNIIRDSISSITNDQRLQVLLIAYSFGAFLEGAAGLGVPVAITAALLVGMGFNPLKAAGIC
LVANIAGGAMGAMGIPVTVPAQLTQLDALTIGRQTVLILPFISIILPFLLVSMVDGIKGIKETWQGILVSGVSFAITQFA
VTYFLGAELTNIFAAVVSMIALALFLRVWQPKSSTKEEKQETKVSHTFNQILYAWSPFVFLTAFVTIFNLKPIKALFAPD
GALANLVFNIQFPGLHNAVMKTTPITKADTPFAAVFKFDVFSSTTTAIVLAIIVSLIVYRVKGKMVKELMIETMKELKAP
VYTICSVIALAYVENYSGMSSTLGLAFSSTGNAFPVLSPILGWIGVFITGSVVSSGSLFAPLQAVTADQLNIVPSTLVAL
NVVGGTMAKMVSPQSVAVACAAVGLVGKESALFKTAMKYSLIFLAVISLLQLNAVFNIF
>Mature_538_residues
STWTQIYDPLNNIWLSALIAALPIFCFIICLMGFKMKSYMAGLYSVIVAIILAIFVYKMPATVAVASAGFGVLSGFYPIC
TIVIAAIFLYKLTVKTEQFNIIRDSISSITNDQRLQVLLIAYSFGAFLEGAAGLGVPVAITAALLVGMGFNPLKAAGICL
VANIAGGAMGAMGIPVTVPAQLTQLDALTIGRQTVLILPFISIILPFLLVSMVDGIKGIKETWQGILVSGVSFAITQFAV
TYFLGAELTNIFAAVVSMIALALFLRVWQPKSSTKEEKQETKVSHTFNQILYAWSPFVFLTAFVTIFNLKPIKALFAPDG
ALANLVFNIQFPGLHNAVMKTTPITKADTPFAAVFKFDVFSSTTTAIVLAIIVSLIVYRVKGKMVKELMIETMKELKAPV
YTICSVIALAYVENYSGMSSTLGLAFSSTGNAFPVLSPILGWIGVFITGSVVSSGSLFAPLQAVTADQLNIVPSTLVALN
VVGGTMAKMVSPQSVAVACAAVGLVGKESALFKTAMKYSLIFLAVISLLQLNAVFNIF

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=548, Percent_Identity=50.3649635036496, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1790031, Length=538, Percent_Identity=49.6282527881041, Blast_Score=499, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 57524; Mature: 57393

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STWTQIYDPLNNIWLSALIAALPIFCFIICLMGFKMKSYMAGLYSVIVAIILAIFVYKM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PATVAVASAGFGVLSGFYPICTIVIAAIFLYKLTVKTEQFNIIRDSISSITNDQRLQVLL
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHEEEE
IAYSFGAFLEGAAGLGVPVAITAALLVGMGFNPLKAAGICLVANIAGGAMGAMGIPVTVP
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCEEEECCCCCCCCCCCCEECC
AQLTQLDALTIGRQTVLILPFISIILPFLLVSMVDGIKGIKETWQGILVSGVSFAITQFA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTYFLGAELTNIFAAVVSMIALALFLRVWQPKSSTKEEKQETKVSHTFNQILYAWSPFVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTAFVTIFNLKPIKALFAPDGALANLVFNIQFPGLHNAVMKTTPITKADTPFAAVFKFDV
HHHHHHHHCCCHHHHHHCCCCCCEEEEEEEECCCCHHHHHCCCCCCCCCCCHHHHEEEHH
FSSTTTAIVLAIIVSLIVYRVKGKMVKELMIETMKELKAPVYTICSVIALAYVENYSGMS
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCC
STLGLAFSSTGNAFPVLSPILGWIGVFITGSVVSSGSLFAPLQAVTADQLNIVPSTLVAL
HHHCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHH
NVVGGTMAKMVSPQSVAVACAAVGLVGKESALFKTAMKYSLIFLAVISLLQLNAVFNIF
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]