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The map label for this gene is ilvD

Identifier: 21672832

GI number: 21672832

Start: 632300

End: 634153

Strand: Reverse

Name: ilvD

Synonym: BUsg576

Alternate gene names: 21672832

Gene position: 634153-632300 (Counterclockwise)

Preceding gene: 21672838

Following gene: 21672831

Centisome position: 98.86

GC content: 30.42

Gene sequence:

>1854_bases
ATGCCTAAATATCGTTCATTTACAACTACACATGGTAGAAATATGTCTGGAGCGAGATCCCTGTGGCGTGCTACAGGAAT
GACAGATGAAGACTTTAAAAAACCTATTATTGCAATAGTAAATTCATTTTCTCAATTTGTACCAGGACATATTCATTTAC
AAGAAGTTGGAAAAATAATCTCTAAAGAAATAAAAAAATATGGTGGCGTTCCAAAAGAATTTAATACTATTGCAATAGAT
GATGGAATAGCAATGGGACATTCAGGAATGTTATATTCTCTACCGTCTCGTGAATTAATTGCAGATTCTATAGAATACGT
AATTAATGCTCATTGTGTCGATTCAATGATCTGTGTTTCAAATTGCGATAAAATAACTCCAGCTATGTTTATGGCATCTT
TACGTTTAAATATACCATCAGTCTTTGTTTCAGGAGGACCAATGGAAGCAGGTAAAATAAAAACAAAAGATAAAACAAAA
AAAATTGATTTAGTTGATGCCATAATACATGGAGGTGATTCTAATAAATCCGATGATTTTATAAGAGAAATTGAACTTTC
AGCTTGCCCTACTTGCGGTTCCTGTTCAGGTATGTTTACAGCAAATTCTATGAATTGTTTAATAGAAGCTATAGGTTTAA
GTTTACCTGGAAACGGAACACTATTAGCAACTCATATTGATCGTAAAAAATTATTTAAAAAATCTGCACGAAATATCGTT
AAAATTACGAAAGATTATTACCTTAATAACAATAAAAACGTTTTACCACGCAATATAGCAAATAAAGAATCTTTCGAAAA
TGCTATGGCTTTAGATATTGCCATGGGAGGATCAACTAATACTATTTTGCATTTATTAGCTGCAGCATACGAGGGAAAAG
TTGATTTTAAAATGTCAGATATTAACAACCTTTCAAAAAAAATTCCTCATATTTGCAAAGTAGCTCCGAGTACTAATTTG
TATCATGTAGAAGATGTACATCGAGCAGGTGGTGTAATGAGAATTTTAGGAGAATTAAATCGTTCTAATTTATTAAATAA
AAAAACAGAAAACATATTAGGATTGAATTTAGAAAAAACATTAAAAAAATATGATATTTTGTCTACAAAAAATAAAAATG
TAATAAAGATGTTTCATGCAGGTCCAGGTGGTAATCGTACAATTAAACCTTTTTCTCAAAATTATAGATGGAATAAATTA
GACAAAGATCGCGTTAATGGATGTATTCGTTCACATGAAAATGCCTATAGTAAAAATGGTGGATTATCTGTTTTGTATGG
AAATTTAGCAAAAAATGGATGTATAATAAAAACAGCTAGCATAGATAAAAAACATTATATTTTTTCTGGACCTGCTAAGG
TTTATGAAAGTCAAGAAGAAGCTGTTAGTGCTATTTTATCAGGTAAAATTATTGCTGGTGATGTTATCGTTATTCGATAT
GAAGGTCCCAAGGGTGGGCCCGGAATGCAAGAAATGCTATATCCAACAACATATTTAAAATCTATAAATTTAGATAAACA
ATGTGCATTAATCACCGACGGACGTTTTTCTGGAGGAACATCTGGTTTATCTATAGGTCATATTTCGCCTGAAGCAGCAA
ATAAGGGTATTATCGCTTTAGTAGAAAATGGTGATGATATTCAAATTAATATTGTAAACAAGACAATTCATTTAAATATT
ACAGAAAAAGAACTAAATCTTCGTATCATTAAAGAAAATGCAAAAGGTTTAAAAGCATATAAACCTCTTAATCGCAAAAG
AAATATTTCTTTTGCCCTTCGTGCCTATGCACATTTTGCTTTGAGTGCAGATAAAGGTGCAATAAGAAATAAAAAGAAAC
TATTTAATTTTTAA

Upstream 100 bases:

>100_bases
ATTAAAAAAAATAAAAAAAAGTCTTTAAACTAAAAAACACATACTTATTTTTAAAAATTTCTAAAAATAATAAAAAATAT
TTCTTGGAGTAATTTTTAAA

Downstream 100 bases:

>100_bases
AAAATAAAATTTATCATCAATAACAATAAATATTAAATCAATTTTAAATTATTCAGGAATTACTAAAAATGAATTATTTT
AATAAATTATGTTTTCGTAA

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MPKYRSFTTTHGRNMSGARSLWRATGMTDEDFKKPIIAIVNSFSQFVPGHIHLQEVGKIISKEIKKYGGVPKEFNTIAID
DGIAMGHSGMLYSLPSRELIADSIEYVINAHCVDSMICVSNCDKITPAMFMASLRLNIPSVFVSGGPMEAGKIKTKDKTK
KIDLVDAIIHGGDSNKSDDFIREIELSACPTCGSCSGMFTANSMNCLIEAIGLSLPGNGTLLATHIDRKKLFKKSARNIV
KITKDYYLNNNKNVLPRNIANKESFENAMALDIAMGGSTNTILHLLAAAYEGKVDFKMSDINNLSKKIPHICKVAPSTNL
YHVEDVHRAGGVMRILGELNRSNLLNKKTENILGLNLEKTLKKYDILSTKNKNVIKMFHAGPGGNRTIKPFSQNYRWNKL
DKDRVNGCIRSHENAYSKNGGLSVLYGNLAKNGCIIKTASIDKKHYIFSGPAKVYESQEEAVSAILSGKIIAGDVIVIRY
EGPKGGPGMQEMLYPTTYLKSINLDKQCALITDGRFSGGTSGLSIGHISPEAANKGIIALVENGDDIQINIVNKTIHLNI
TEKELNLRIIKENAKGLKAYKPLNRKRNISFALRAYAHFALSADKGAIRNKKKLFNF

Sequences:

>Translated_617_residues
MPKYRSFTTTHGRNMSGARSLWRATGMTDEDFKKPIIAIVNSFSQFVPGHIHLQEVGKIISKEIKKYGGVPKEFNTIAID
DGIAMGHSGMLYSLPSRELIADSIEYVINAHCVDSMICVSNCDKITPAMFMASLRLNIPSVFVSGGPMEAGKIKTKDKTK
KIDLVDAIIHGGDSNKSDDFIREIELSACPTCGSCSGMFTANSMNCLIEAIGLSLPGNGTLLATHIDRKKLFKKSARNIV
KITKDYYLNNNKNVLPRNIANKESFENAMALDIAMGGSTNTILHLLAAAYEGKVDFKMSDINNLSKKIPHICKVAPSTNL
YHVEDVHRAGGVMRILGELNRSNLLNKKTENILGLNLEKTLKKYDILSTKNKNVIKMFHAGPGGNRTIKPFSQNYRWNKL
DKDRVNGCIRSHENAYSKNGGLSVLYGNLAKNGCIIKTASIDKKHYIFSGPAKVYESQEEAVSAILSGKIIAGDVIVIRY
EGPKGGPGMQEMLYPTTYLKSINLDKQCALITDGRFSGGTSGLSIGHISPEAANKGIIALVENGDDIQINIVNKTIHLNI
TEKELNLRIIKENAKGLKAYKPLNRKRNISFALRAYAHFALSADKGAIRNKKKLFNF
>Mature_616_residues
PKYRSFTTTHGRNMSGARSLWRATGMTDEDFKKPIIAIVNSFSQFVPGHIHLQEVGKIISKEIKKYGGVPKEFNTIAIDD
GIAMGHSGMLYSLPSRELIADSIEYVINAHCVDSMICVSNCDKITPAMFMASLRLNIPSVFVSGGPMEAGKIKTKDKTKK
IDLVDAIIHGGDSNKSDDFIREIELSACPTCGSCSGMFTANSMNCLIEAIGLSLPGNGTLLATHIDRKKLFKKSARNIVK
ITKDYYLNNNKNVLPRNIANKESFENAMALDIAMGGSTNTILHLLAAAYEGKVDFKMSDINNLSKKIPHICKVAPSTNLY
HVEDVHRAGGVMRILGELNRSNLLNKKTENILGLNLEKTLKKYDILSTKNKNVIKMFHAGPGGNRTIKPFSQNYRWNKLD
KDRVNGCIRSHENAYSKNGGLSVLYGNLAKNGCIIKTASIDKKHYIFSGPAKVYESQEEAVSAILSGKIIAGDVIVIRYE
GPKGGPGMQEMLYPTTYLKSINLDKQCALITDGRFSGGTSGLSIGHISPEAANKGIIALVENGDDIQINIVNKTIHLNIT
EKELNLRIIKENAKGLKAYKPLNRKRNISFALRAYAHFALSADKGAIRNKKKLFNF

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=614, Percent_Identity=67.9153094462541, Blast_Score=919, Evalue=0.0,
Organism=Escherichia coli, GI1788157, Length=551, Percent_Identity=28.4936479128857, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI2367371, Length=524, Percent_Identity=28.4351145038168, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1786464, Length=533, Percent_Identity=27.3921200750469, Blast_Score=166, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6322476, Length=570, Percent_Identity=36.3157894736842, Blast_Score=317, Evalue=4e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_BUCAP (O51887)

Other databases:

- EMBL:   AF008210
- EMBL:   AE013218
- RefSeq:   NP_660899.1
- EnsemblBacteria:   EBBUCT00000000173
- GeneID:   1005621
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg576
- GeneTree:   EBGT00050000008100
- HOGENOM:   HBG671001
- OMA:   KVPCLSK
- ProtClustDB:   PRK12448
- BioCyc:   BAPH198804:BUSG576-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 67861; Mature: 67729

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKYRSFTTTHGRNMSGARSLWRATGMTDEDFKKPIIAIVNSFSQFVPGHIHLQEVGKII
CCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHH
SKEIKKYGGVPKEFNTIAIDDGIAMGHSGMLYSLPSRELIADSIEYVINAHCVDSMICVS
HHHHHHHCCCCCCCCEEEEECCEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPAMFMASLRLNIPSVFVSGGPMEAGKIKTKDKTKKIDLVDAIIHGGDSNKSDDF
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCHH
IREIELSACPTCGSCSGMFTANSMNCLIEAIGLSLPGNGTLLATHIDRKKLFKKSARNIV
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHE
KITKDYYLNNNKNVLPRNIANKESFENAMALDIAMGGSTNTILHLLAAAYEGKVDFKMSD
EEEHHEEECCCCCCCCCCCCCHHHHCCEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEHH
INNLSKKIPHICKVAPSTNLYHVEDVHRAGGVMRILGELNRSNLLNKKTENILGLNLEKT
HHHHHHHCCHHEEECCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEECCCHHHH
LKKYDILSTKNKNVIKMFHAGPGGNRTIKPFSQNYRWNKLDKDRVNGCIRSHENAYSKNG
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
GLSVLYGNLAKNGCIIKTASIDKKHYIFSGPAKVYESQEEAVSAILSGKIIAGDVIVIRY
CEEEEECCCCCCCEEEEEECCCCCEEEECCHHHHHCCHHHHHHHHHCCCEEECCEEEEEE
EGPKGGPGMQEMLYPTTYLKSINLDKQCALITDGRFSGGTSGLSIGHISPEAANKGIIAL
CCCCCCCCHHHHHCHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCHHHCCCCEEEE
VENGDDIQINIVNKTIHLNITEKELNLRIIKENAKGLKAYKPLNRKRNISFALRAYAHFA
EECCCEEEEEEEEEEEEEEEECCCCEEEEEECCCCCCHHHCCCCCCCCCHHEEEEHHHHE
LSADKGAIRNKKKLFNF
EECCCCHHCCCHHHCCC
>Mature Secondary Structure 
PKYRSFTTTHGRNMSGARSLWRATGMTDEDFKKPIIAIVNSFSQFVPGHIHLQEVGKII
CCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHH
SKEIKKYGGVPKEFNTIAIDDGIAMGHSGMLYSLPSRELIADSIEYVINAHCVDSMICVS
HHHHHHHCCCCCCCCEEEEECCEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPAMFMASLRLNIPSVFVSGGPMEAGKIKTKDKTKKIDLVDAIIHGGDSNKSDDF
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCHH
IREIELSACPTCGSCSGMFTANSMNCLIEAIGLSLPGNGTLLATHIDRKKLFKKSARNIV
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHE
KITKDYYLNNNKNVLPRNIANKESFENAMALDIAMGGSTNTILHLLAAAYEGKVDFKMSD
EEEHHEEECCCCCCCCCCCCCHHHHCCEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEHH
INNLSKKIPHICKVAPSTNLYHVEDVHRAGGVMRILGELNRSNLLNKKTENILGLNLEKT
HHHHHHHCCHHEEECCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEECCCHHHH
LKKYDILSTKNKNVIKMFHAGPGGNRTIKPFSQNYRWNKLDKDRVNGCIRSHENAYSKNG
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
GLSVLYGNLAKNGCIIKTASIDKKHYIFSGPAKVYESQEEAVSAILSGKIIAGDVIVIRY
CEEEEECCCCCCCEEEEEECCCCCEEEECCHHHHHCCHHHHHHHHHCCCEEECCEEEEEE
EGPKGGPGMQEMLYPTTYLKSINLDKQCALITDGRFSGGTSGLSIGHISPEAANKGIIAL
CCCCCCCCHHHHHCHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCHHHCCCCEEEE
VENGDDIQINIVNKTIHLNITEKELNLRIIKENAKGLKAYKPLNRKRNISFALRAYAHFA
EECCCEEEEEEEEEEEEEEEECCCCEEEEEECCCCCCHHHCCCCCCCCCHHEEEEHHHHE
LSADKGAIRNKKKLFNF
EECCCCHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9516544; 12089438