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The map label for this gene is pitA

Identifier: 21672824

GI number: 21672824

Start: 620508

End: 621989

Strand: Reverse

Name: pitA

Synonym: BUsg566

Alternate gene names: 21672824

Gene position: 621989-620508 (Counterclockwise)

Preceding gene: 21672825

Following gene: 21672822

Centisome position: 96.97

GC content: 25.71

Gene sequence:

>1482_bases
ATGCTATATTTATTTTCTTATTCTGATTTGAATCATAGTTTATTTATTTTACTAGCTTTATTTTTTGTATTGTGTTACGA
AGCTATTAATGGATTTCATGATACAGCAAATGCTGTGTCAACATTGATTTATACACGTGCAACTTCAGCTCATTTTGCAG
TTATAATGTCAGGTCTATTTAATTTTCTTGGTGTCTTGTTGGGAGGTTTAACAGTTGCGTATGCAATTGTTCATTTATTG
CCAAATGATTTACTATTAAATAGTAATTCAAAAAATGCTCTTGCTATGGTTTTTTCTATGTTATTAGCAGCTATACTATG
GAATTTATCTACTTGGTATTTTTGTTTACCAGCTTCAAGTTCGCATTCGCTTATTGGAGCAATAATTGGAATTGGTTTAA
CTAACGCGATAATTACTGATTCTTCTTTATTACATGCTTTAAATGTTCCTAAAATGACAAATGTATTTTTATCTCTTATT
TTTTCACCGATTGTAGGATTAATAATTTCGGGTAGTTTAATATTTTTGTTACGTTTTTTTTTAAAAAAGAAAAAGAATCA
TTATCGTATTCATATGACTCCATTAGAAAGAGAACAAAAAGAAGGCAAAAAAAAACCACCTTTTTTAATTAAAATCGCTT
TGATCTTATCATCTATTGGAGTAAGTTATGCACATGGTGCAAACGATGGTCAAAAAGGAATTGGTTTGTTAATGCTTGTC
CTTATCGGAATTGCACCATGTGGTTTTTTAGTTAATTTAAATGCATCTAAAAAAGAAATTATTTACACAAAAAATACAAT
TGATCATCTATATGAATACTATTCAAAAAATAAAGTCAAAATAATTAAAAATACTAAAAATCAAAAAAAAATTGATTTTT
CAATAAAAAATTATAATTATTTTGAAATTATAAAAAATATAAAAAAAACTAAATTTTTATTACAAAATATATATGATTTT
AATCAATTAAATATAAAACAAAGATTTAAATTACGTCATTTTTTACTTTTTCTTTCTGAAAATATAGATCAAACAATACA
TTTTTCAAATATCGTTAAAAAAGAAAAAGATTTTTTAAAAAAAAGTAAAAAAGATATACTTAAAACGATTGAATATGCAC
CAATGTGGATAATACTAATTATCGCACTTTCTTTATCTGTAGGTACAATGATTGGTTGGAAACGTATAGTAGTAACTATT
GGTGAAAAGATAGGAAAAAAAAGAATGACATATGCACAAGCCATGTCGGCGCAGATAACTGCTTCTTTATCAATTGGTAT
AGCAAGCTATACAGGAATACCTGTATCTACAACACATATTTTGTCTTCTTCTGTAGCTGGAGCAATGTTAAGTGATGGAG
ATGGTATTCAAATTAGTACAATAAAAAATATTGCTCTTGCTTGGATATTAACTTTACCTGTTTCAATTTTATTGTCTGGT
TTTTTTTATTGGATTACTCTTTTATTTATAAAAAATTTTTAA

Upstream 100 bases:

>100_bases
TTAAAAAAATAATATAGTTTTTTAAAATAATTACTTTCCTCTAACAAGGTTTCTCACAGATAATAAACTTAGTTCAATTC
ATCAAAATGGCTTAAAGGTT

Downstream 100 bases:

>100_bases
AAGATTTATGACATTTTAATTATAGGAGTACGTTTTAAAAAAATGTACTCCTATTTTTTTTTAAAAGGTAGATATATATC
AAAACGATGATATTTATTAC

Product: low-affinity inorganic phosphate transporter 1

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MLYLFSYSDLNHSLFILLALFFVLCYEAINGFHDTANAVSTLIYTRATSAHFAVIMSGLFNFLGVLLGGLTVAYAIVHLL
PNDLLLNSNSKNALAMVFSMLLAAILWNLSTWYFCLPASSSHSLIGAIIGIGLTNAIITDSSLLHALNVPKMTNVFLSLI
FSPIVGLIISGSLIFLLRFFLKKKKNHYRIHMTPLEREQKEGKKKPPFLIKIALILSSIGVSYAHGANDGQKGIGLLMLV
LIGIAPCGFLVNLNASKKEIIYTKNTIDHLYEYYSKNKVKIIKNTKNQKKIDFSIKNYNYFEIIKNIKKTKFLLQNIYDF
NQLNIKQRFKLRHFLLFLSENIDQTIHFSNIVKKEKDFLKKSKKDILKTIEYAPMWIILIIALSLSVGTMIGWKRIVVTI
GEKIGKKRMTYAQAMSAQITASLSIGIASYTGIPVSTTHILSSSVAGAMLSDGDGIQISTIKNIALAWILTLPVSILLSG
FFYWITLLFIKNF

Sequences:

>Translated_493_residues
MLYLFSYSDLNHSLFILLALFFVLCYEAINGFHDTANAVSTLIYTRATSAHFAVIMSGLFNFLGVLLGGLTVAYAIVHLL
PNDLLLNSNSKNALAMVFSMLLAAILWNLSTWYFCLPASSSHSLIGAIIGIGLTNAIITDSSLLHALNVPKMTNVFLSLI
FSPIVGLIISGSLIFLLRFFLKKKKNHYRIHMTPLEREQKEGKKKPPFLIKIALILSSIGVSYAHGANDGQKGIGLLMLV
LIGIAPCGFLVNLNASKKEIIYTKNTIDHLYEYYSKNKVKIIKNTKNQKKIDFSIKNYNYFEIIKNIKKTKFLLQNIYDF
NQLNIKQRFKLRHFLLFLSENIDQTIHFSNIVKKEKDFLKKSKKDILKTIEYAPMWIILIIALSLSVGTMIGWKRIVVTI
GEKIGKKRMTYAQAMSAQITASLSIGIASYTGIPVSTTHILSSSVAGAMLSDGDGIQISTIKNIALAWILTLPVSILLSG
FFYWITLLFIKNF
>Mature_493_residues
MLYLFSYSDLNHSLFILLALFFVLCYEAINGFHDTANAVSTLIYTRATSAHFAVIMSGLFNFLGVLLGGLTVAYAIVHLL
PNDLLLNSNSKNALAMVFSMLLAAILWNLSTWYFCLPASSSHSLIGAIIGIGLTNAIITDSSLLHALNVPKMTNVFLSLI
FSPIVGLIISGSLIFLLRFFLKKKKNHYRIHMTPLEREQKEGKKKPPFLIKIALILSSIGVSYAHGANDGQKGIGLLMLV
LIGIAPCGFLVNLNASKKEIIYTKNTIDHLYEYYSKNKVKIIKNTKNQKKIDFSIKNYNYFEIIKNIKKTKFLLQNIYDF
NQLNIKQRFKLRHFLLFLSENIDQTIHFSNIVKKEKDFLKKSKKDILKTIEYAPMWIILIIALSLSVGTMIGWKRIVVTI
GEKIGKKRMTYAQAMSAQITASLSIGIASYTGIPVSTTHILSSSVAGAMLSDGDGIQISTIKNIALAWILTLPVSILLSG
FFYWITLLFIKNF

Specific function: Low-affinity inorganic phosphate transport

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily

Homologues:

Organism=Escherichia coli, GI1789907, Length=501, Percent_Identity=56.2874251497006, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI1789360, Length=501, Percent_Identity=54.6906187624751, Blast_Score=525, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI25146401, Length=505, Percent_Identity=22.5742574257426, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIT_BUCAP (Q8K903)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660891.1
- EnsemblBacteria:   EBBUCT00000000013
- GeneID:   1005562
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg566
- GeneTree:   EBGT00050000007843
- HOGENOM:   HBG681698
- OMA:   GQLRRIM
- ProtClustDB:   CLSK316561
- BioCyc:   BAPH198804:BUSG566-MONOMER
- InterPro:   IPR001204
- PANTHER:   PTHR11101

Pfam domain/function: PF01384 PHO4

EC number: NA

Molecular weight: Translated: 55217; Mature: 55217

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc9a1294)-; HASH(0xc1aeb54)-; HASH(0xc3263e8)-; HASH(0xcd7ff7c)-; HASH(0xce11adc)-; HASH(0xbf832dc)-; HASH(0xb623e54)-; HASH(0x95a839c)-; HASH(0xcc9ca00)-; HASH(0xcdd5e14)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYLFSYSDLNHSLFILLALFFVLCYEAINGFHDTANAVSTLIYTRATSAHFAVIMSGLF
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
NFLGVLLGGLTVAYAIVHLLPNDLLLNSNSKNALAMVFSMLLAAILWNLSTWYFCLPASS
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
SHSLIGAIIGIGLTNAIITDSSLLHALNVPKMTNVFLSLIFSPIVGLIISGSLIFLLRFF
CCHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKKKKNHYRIHMTPLEREQKEGKKKPPFLIKIALILSSIGVSYAHGANDGQKGIGLLMLV
HHHCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
LIGIAPCGFLVNLNASKKEIIYTKNTIDHLYEYYSKNKVKIIKNTKNQKKIDFSIKNYNY
HHHHCCCCEEEECCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCHH
FEIIKNIKKTKFLLQNIYDFNQLNIKQRFKLRHFLLFLSENIDQTIHFSNIVKKEKDFLK
HHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KSKKDILKTIEYAPMWIILIIALSLSVGTMIGWKRIVVTIGEKIGKKRMTYAQAMSAQIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHE
ASLSIGIASYTGIPVSTTHILSSSVAGAMLSDGDGIQISTIKNIALAWILTLPVSILLSG
EEEEEEEHHCCCCCCCHHHHHHHHHCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
FFYWITLLFIKNF
HHHHHHHHHHHCH
>Mature Secondary Structure
MLYLFSYSDLNHSLFILLALFFVLCYEAINGFHDTANAVSTLIYTRATSAHFAVIMSGLF
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
NFLGVLLGGLTVAYAIVHLLPNDLLLNSNSKNALAMVFSMLLAAILWNLSTWYFCLPASS
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
SHSLIGAIIGIGLTNAIITDSSLLHALNVPKMTNVFLSLIFSPIVGLIISGSLIFLLRFF
CCHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKKKKNHYRIHMTPLEREQKEGKKKPPFLIKIALILSSIGVSYAHGANDGQKGIGLLMLV
HHHCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
LIGIAPCGFLVNLNASKKEIIYTKNTIDHLYEYYSKNKVKIIKNTKNQKKIDFSIKNYNY
HHHHCCCCEEEECCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCHH
FEIIKNIKKTKFLLQNIYDFNQLNIKQRFKLRHFLLFLSENIDQTIHFSNIVKKEKDFLK
HHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KSKKDILKTIEYAPMWIILIIALSLSVGTMIGWKRIVVTIGEKIGKKRMTYAQAMSAQIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHE
ASLSIGIASYTGIPVSTTHILSSSVAGAMLSDGDGIQISTIKNIALAWILTLPVSILLSG
EEEEEEEHHCCCCCCCHHHHHHHHHCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
FFYWITLLFIKNF
HHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12089438