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The map label for this gene is purA

Identifier: 21672807

GI number: 21672807

Start: 598805

End: 600097

Strand: Reverse

Name: purA

Synonym: BUsg546

Alternate gene names: 21672807

Gene position: 600097-598805 (Counterclockwise)

Preceding gene: 21672808

Following gene: 21672806

Centisome position: 93.55

GC content: 28.92

Gene sequence:

>1293_bases
ATGAATACAAACATTGTAATATTAGGTACTCAATGGGGTGATGAAGGTAAAGGAAAAATAGTTGATTTTTTGTCTTCAAA
TAGTTCTTATGTAGTAAGATATCATGGAGGACATAACGCTGGTCATACCTTAGTAGTTAATGGAAAAAAAATTATTCTTC
ATTTAATTCCATCAGGATTACTATATCCTAATGTCATTGGAATTATTTCAAATGGAGTAGTAATTTCTCCATTTGAGTTA
GTTAAAGAAATAAGAATGCTAGAGAAAAACAACCATTTTATTAATGAACGTCTTTTTATTTCTGAATCAGCTCCTTTAAT
ATTACCCTATCATATTGCAATAGACTTAGCTCGTGAAAAACAATTAGGAATCAATGCAATTGGTACGACGGGTCGTGGTA
TTGGTCCTGCATATGAAGATAAAGTAGCACGTCGCGCTTTACGAGTAGGAGATTTAAAAAACACACAAAAATTATCTACA
AAATTAAAAGTAATAGTTGATTATTATAATCATCAATTGGTATCATTTTACAAACAAAATCCAATCTCTTATAAAGAGAT
CTTAAAAGATTTATTAAAAGTAAAAGATCTAATCTACAATATGATTAAAGATACTTCTGCTATTTTACATAAAGCAATTG
AAGATAAAAAAAGAATTATTTTTGAAGGTGCTCAAGGTACTCTTTTAGATATTGATCACGGTACATATCCATATGTTACG
TCTTCTAATAGTACTACAGGTGGTATAATTACTGGTACAGGAGTTGGGCCTAAAAATTTAGGTTATATATTAGGAATAAC
TAAAGCATATACAACAAGAGTTGGAAAAGGACCATTTCCAACTGAATTGTTTGATGATATAGATAGTTATCTTTCTAAAA
AAGGACAAGAATTCGGATCTACAACTGGTCGAAAAAGACGTACTGGATGGCTTGATGGTGTAGCTTTACGCTATTCCGTT
AAAATTAATTCTTTATCTGCATTATGCATAACAAAGTTAGATGTTTTAGATGATTTAAAAGAAATAAAAATTTGTACATC
TTATAAAGATGTCAATACTAAAAAAATTTATAAAGATTTTCCTATTTTTAATTTAGAAGATGTCACTCCAGTATATGAAG
TTCATCCAGGATGGATGAAAAAAACATCAGGTATAAAGAAAATAGAAGATTTACCTAAAGCAGCTTGTAATTATATAAAT
CGTATTGAAGAAATAGCACAAGTTCCTATTGATATAATTTCTACCGGTCCAGATCGTTCTGATACTATTTTGTTAAAAAG
TCTATTTTTTTAA

Upstream 100 bases:

>100_bases
ATGTTAATTAATTCAGATAACGAGTTTTTTAAATATATGAATACAGAAATCAAAAATTAAAAGTACATTTCAAAAAATTG
ATAAAATAGGTATTAAAAAT

Downstream 100 bases:

>100_bases
ATTATGATTTTTAATCATCATTTTTAAATTTTTTATAAAATATGCTAAAAAAATAAGATTTTTAAATAAATTTGACGATT
ATTAATTGATGATAAATTAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNTNIVILGTQWGDEGKGKIVDFLSSNSSYVVRYHGGHNAGHTLVVNGKKIILHLIPSGLLYPNVIGIISNGVVISPFEL
VKEIRMLEKNNHFINERLFISESAPLILPYHIAIDLAREKQLGINAIGTTGRGIGPAYEDKVARRALRVGDLKNTQKLST
KLKVIVDYYNHQLVSFYKQNPISYKEILKDLLKVKDLIYNMIKDTSAILHKAIEDKKRIIFEGAQGTLLDIDHGTYPYVT
SSNSTTGGIITGTGVGPKNLGYILGITKAYTTRVGKGPFPTELFDDIDSYLSKKGQEFGSTTGRKRRTGWLDGVALRYSV
KINSLSALCITKLDVLDDLKEIKICTSYKDVNTKKIYKDFPIFNLEDVTPVYEVHPGWMKKTSGIKKIEDLPKAACNYIN
RIEEIAQVPIDIISTGPDRSDTILLKSLFF

Sequences:

>Translated_430_residues
MNTNIVILGTQWGDEGKGKIVDFLSSNSSYVVRYHGGHNAGHTLVVNGKKIILHLIPSGLLYPNVIGIISNGVVISPFEL
VKEIRMLEKNNHFINERLFISESAPLILPYHIAIDLAREKQLGINAIGTTGRGIGPAYEDKVARRALRVGDLKNTQKLST
KLKVIVDYYNHQLVSFYKQNPISYKEILKDLLKVKDLIYNMIKDTSAILHKAIEDKKRIIFEGAQGTLLDIDHGTYPYVT
SSNSTTGGIITGTGVGPKNLGYILGITKAYTTRVGKGPFPTELFDDIDSYLSKKGQEFGSTTGRKRRTGWLDGVALRYSV
KINSLSALCITKLDVLDDLKEIKICTSYKDVNTKKIYKDFPIFNLEDVTPVYEVHPGWMKKTSGIKKIEDLPKAACNYIN
RIEEIAQVPIDIISTGPDRSDTILLKSLFF
>Mature_430_residues
MNTNIVILGTQWGDEGKGKIVDFLSSNSSYVVRYHGGHNAGHTLVVNGKKIILHLIPSGLLYPNVIGIISNGVVISPFEL
VKEIRMLEKNNHFINERLFISESAPLILPYHIAIDLAREKQLGINAIGTTGRGIGPAYEDKVARRALRVGDLKNTQKLST
KLKVIVDYYNHQLVSFYKQNPISYKEILKDLLKVKDLIYNMIKDTSAILHKAIEDKKRIIFEGAQGTLLDIDHGTYPYVT
SSNSTTGGIITGTGVGPKNLGYILGITKAYTTRVGKGPFPTELFDDIDSYLSKKGQEFGSTTGRKRRTGWLDGVALRYSV
KINSLSALCITKLDVLDDLKEIKICTSYKDVNTKKIYKDFPIFNLEDVTPVYEVHPGWMKKTSGIKKIEDLPKAACNYIN
RIEEIAQVPIDIISTGPDRSDTILLKSLFF

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=429, Percent_Identity=43.8228438228438, Blast_Score=328, Evalue=4e-90,
Organism=Homo sapiens, GI34577063, Length=435, Percent_Identity=42.5287356321839, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=43.1077694235589, Blast_Score=290, Evalue=2e-78,
Organism=Escherichia coli, GI1790620, Length=434, Percent_Identity=58.294930875576, Blast_Score=538, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=41.6279069767442, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI25146555, Length=433, Percent_Identity=41.3394919168591, Blast_Score=314, Evalue=5e-86,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=43.6194895591647, Blast_Score=327, Evalue=2e-90,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=42.1800947867299, Blast_Score=329, Evalue=2e-90,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_BUCAP (Q8K916)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660874.1
- ProteinModelPortal:   Q8K916
- SMR:   Q8K916
- EnsemblBacteria:   EBBUCT00000000437
- GeneID:   1005641
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg546
- GeneTree:   EBGT00050000007897
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- ProtClustDB:   PRK01117
- BioCyc:   BAPH198804:BUSG546-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47922; Mature: 47922

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNIVILGTQWGDEGKGKIVDFLSSNSSYVVRYHGGHNAGHTLVVNGKKIILHLIPSGL
CCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEECCCEEEEEECCCCC
LYPNVIGIISNGVVISPFELVKEIRMLEKNNHFINERLFISESAPLILPYHIAIDLAREK
CCHHHHHHHCCCEEECHHHHHHHHHHHHCCCCEECCEEEEECCCCEEEEEEEEEEEHHHH
QLGINAIGTTGRGIGPAYEDKVARRALRVGDLKNTQKLSTKLKVIVDYYNHQLVSFYKQN
CCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCC
PISYKEILKDLLKVKDLIYNMIKDTSAILHKAIEDKKRIIFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCCCCCEEE
SSNSTTGGIITGTGVGPKNLGYILGITKAYTTRVGKGPFPTELFDDIDSYLSKKGQEFGS
CCCCCCCCEEEECCCCCCCCCEEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHCC
TTGRKRRTGWLDGVALRYSVKINSLSALCITKLDVLDDLKEIKICTSYKDVNTKKIYKDF
CCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCC
PIFNLEDVTPVYEVHPGWMKKTSGIKKIEDLPKAACNYINRIEEIAQVPIDIISTGPDRS
CCCCCCCCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
DTILLKSLFF
CEEHHHHHCC
>Mature Secondary Structure
MNTNIVILGTQWGDEGKGKIVDFLSSNSSYVVRYHGGHNAGHTLVVNGKKIILHLIPSGL
CCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEECCCEEEEEECCCCC
LYPNVIGIISNGVVISPFELVKEIRMLEKNNHFINERLFISESAPLILPYHIAIDLAREK
CCHHHHHHHCCCEEECHHHHHHHHHHHHCCCCEECCEEEEECCCCEEEEEEEEEEEHHHH
QLGINAIGTTGRGIGPAYEDKVARRALRVGDLKNTQKLSTKLKVIVDYYNHQLVSFYKQN
CCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCC
PISYKEILKDLLKVKDLIYNMIKDTSAILHKAIEDKKRIIFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCCCCCEEE
SSNSTTGGIITGTGVGPKNLGYILGITKAYTTRVGKGPFPTELFDDIDSYLSKKGQEFGS
CCCCCCCCEEEECCCCCCCCCEEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHCC
TTGRKRRTGWLDGVALRYSVKINSLSALCITKLDVLDDLKEIKICTSYKDVNTKKIYKDF
CCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCC
PIFNLEDVTPVYEVHPGWMKKTSGIKKIEDLPKAACNYINRIEEIAQVPIDIISTGPDRS
CCCCCCCCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
DTILLKSLFF
CEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438