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The map label for this gene is rlmL

Identifier: 21672626

GI number: 21672626

Start: 397315

End: 399408

Strand: Direct

Name: rlmL

Synonym: BUsg351

Alternate gene names: 21672626

Gene position: 397315-399408 (Clockwise)

Preceding gene: 21672625

Following gene: 21672627

Centisome position: 61.94

GC content: 23.64

Gene sequence:

>2094_bases
ATGAATTATTTATTTGCTAGTACAAATTTTGGATGTGAAGAATTATTAGAAAAAGAACTCCGAGCATTGGGAGCTAAAAA
TTTAAAAGTTGTAAAAGGCGGTATCTATTACGAAGGAGAAGATTCAATACTATATAATAGTTTAATGTGGAGCCGAATTG
CTTCAAGAATTTTTTTATGTATAAAAAAATTTACTATAAAAAATAGTAATGATTTATATAAAAACACCTATAACATTAAT
TGGACCCAAATATTATACTTAGAAAAAACTTTTTTAGTTAAATTTAGGGGGACTAATAATATTATTCGAAATAGTTTATT
TGGTGCATTAAAAATTAAAGATGCAATTGTTGATACATTTTACCAAAAATATTCAGCTCGTCCTAACATAAATCTTCTTA
CTCCTGATGTTCGTATCATATCATATTTATGTCAAAATTTAGTGCATATTATGTTAGATTTAAGTGGTGAAGCTCTAAAT
AAAAGAGGATATCGTAAATTTTTTAACATTTCTCCTATCAAAGAAAATTTAAGTGCAGCAATTATATTAAGTTCAGGATG
GAAAAAAAATACTCCTTTAATAGATCCTATGTGTGGATCAGGAACATTATTAATTGAAGCAGCCATGATTTCTTCTGATC
GAGCTCCTGGACTAAAAAGAACAGAATGGGGATTTAAATCTTGGAAAGGACACAAGGAAAAAGTCTGGAAAGAAACTCTA
AAAAAAGCAAAAGAAAGATTTAAAATAGGTATAAAAAAATGTCTTAAAAATTACTTTATAGGATATGATTGTAATCCCGA
TGTTATCAAAAAAGCTCAAAAAAATGTAATAAATGCAAATCTAGAAAATATAATTAGTTTTTTTGCATGTAATTTAAGTC
ATCTTAAAAATCCTTATCAAAAAGAAGAAATCGGCACATTAATAAGTAATCCTCCATATGGAGAAAGATGTAAAACCGAA
AATAATTTAATAGCTTTGTATATTGAATTAGGAATTATGTCAAAAAAATATTTTGAAAAATGGAATTTATCAGTATTTAG
TTCTTCTGAATTTTTATTAAATTTTTTACAAATGAAATCATATAAAAACTTTTGTTTAAAAAATGGACCGTTATATTGTA
CTTTAAAAAACTACGAAATATTTTTAAATAAATTTAATAATACAAATAAAGAATATGAAAATAGATTAGAAAAAAACTTT
AAAAAATTAAAAAAATGGAACGATTTAAAAGAAATAGAATGTTTCCGTGTATATGATTCAGATCTTCCAAATTATAAACT
AATAGTAGATGTTTATAAAAAATGGCTAGTGATTCAAGAATATCAGGCACCAAAATCAATAAACTATGAGAAAGCACATA
AAAGATTATGCAGTGCTATTTACCATAGTAAAGAGATATTATCTATTCCTACAAACAACGTAGTAATAAAATTTAGAAAA
AAACAAAAAAGAAAAGAACAATACCAAAAATTATTTAATAGCAATAGTTTTTTTATAATTAGAGAATATCATGTAAAGTT
ATTAGTTAATTTAATTGATTATTTAGATACTGGTTTATTCTCAGAACACCGACTTGTAAGAAAATTAATAGGATCAATGT
CTAAGGGAAAAGATTTTTTAAATCTATTTTCATATACTGGTGCCGCTAGTGTTTATGCAGGTTTAGGAAAATCTAAAAGT
ATTACTACTGTAGACATATCTAATACGTATATTCAATGGTCTATGCGTAATATGTCTATTAACAACTTAATAAATAGTAA
AAATATTTTTATTCAAAAAGACTGTTTAGAATGGATTATTTCAACTAAAAATAAATTTGATTTAATTTTCATAAATCCGC
CTACTTTTTCTAATTCTAAGAGAATGAAAAAATCTTTCGAATTAAAAAGAGATTATATTAAATTAATGATAAATTTAAAA
CAAATATTACGTAAAGATGGTAACATTATTTTTTCAAGTTCAACACATAATTTTGAAATTGATTTAAACAATATTAAAAA
AATCAACCTATACGCAAAAAAAATTACTAATTTAGTGAAAACAAAAGATTTTTTAAAAAAAAATTATCATTCTTGGTTAA
TAAAACACATTTAA

Upstream 100 bases:

>100_bases
TATTTTAAACAAAAATTACTGATTTTTTATATTAACAAATCTTTTTAGATGTTAATTTTTTTAAAAAATTTTAAATATTT
AATCAAATAAAAGTAAAAAA

Downstream 100 bases:

>100_bases
AAAATTCAAAAAAAAGAGAAATATATGCCTCTAATCAGTATCCAAAATGCCTTTTTAGCATTCAGTGATTTAGAAATCTT
GAAAAATGCTGTACTTTATA

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 697; Mature: 697

Protein sequence:

>697_residues
MNYLFASTNFGCEELLEKELRALGAKNLKVVKGGIYYEGEDSILYNSLMWSRIASRIFLCIKKFTIKNSNDLYKNTYNIN
WTQILYLEKTFLVKFRGTNNIIRNSLFGALKIKDAIVDTFYQKYSARPNINLLTPDVRIISYLCQNLVHIMLDLSGEALN
KRGYRKFFNISPIKENLSAAIILSSGWKKNTPLIDPMCGSGTLLIEAAMISSDRAPGLKRTEWGFKSWKGHKEKVWKETL
KKAKERFKIGIKKCLKNYFIGYDCNPDVIKKAQKNVINANLENIISFFACNLSHLKNPYQKEEIGTLISNPPYGERCKTE
NNLIALYIELGIMSKKYFEKWNLSVFSSSEFLLNFLQMKSYKNFCLKNGPLYCTLKNYEIFLNKFNNTNKEYENRLEKNF
KKLKKWNDLKEIECFRVYDSDLPNYKLIVDVYKKWLVIQEYQAPKSINYEKAHKRLCSAIYHSKEILSIPTNNVVIKFRK
KQKRKEQYQKLFNSNSFFIIREYHVKLLVNLIDYLDTGLFSEHRLVRKLIGSMSKGKDFLNLFSYTGAASVYAGLGKSKS
ITTVDISNTYIQWSMRNMSINNLINSKNIFIQKDCLEWIISTKNKFDLIFINPPTFSNSKRMKKSFELKRDYIKLMINLK
QILRKDGNIIFSSSTHNFEIDLNNIKKINLYAKKITNLVKTKDFLKKNYHSWLIKHI

Sequences:

>Translated_697_residues
MNYLFASTNFGCEELLEKELRALGAKNLKVVKGGIYYEGEDSILYNSLMWSRIASRIFLCIKKFTIKNSNDLYKNTYNIN
WTQILYLEKTFLVKFRGTNNIIRNSLFGALKIKDAIVDTFYQKYSARPNINLLTPDVRIISYLCQNLVHIMLDLSGEALN
KRGYRKFFNISPIKENLSAAIILSSGWKKNTPLIDPMCGSGTLLIEAAMISSDRAPGLKRTEWGFKSWKGHKEKVWKETL
KKAKERFKIGIKKCLKNYFIGYDCNPDVIKKAQKNVINANLENIISFFACNLSHLKNPYQKEEIGTLISNPPYGERCKTE
NNLIALYIELGIMSKKYFEKWNLSVFSSSEFLLNFLQMKSYKNFCLKNGPLYCTLKNYEIFLNKFNNTNKEYENRLEKNF
KKLKKWNDLKEIECFRVYDSDLPNYKLIVDVYKKWLVIQEYQAPKSINYEKAHKRLCSAIYHSKEILSIPTNNVVIKFRK
KQKRKEQYQKLFNSNSFFIIREYHVKLLVNLIDYLDTGLFSEHRLVRKLIGSMSKGKDFLNLFSYTGAASVYAGLGKSKS
ITTVDISNTYIQWSMRNMSINNLINSKNIFIQKDCLEWIISTKNKFDLIFINPPTFSNSKRMKKSFELKRDYIKLMINLK
QILRKDGNIIFSSSTHNFEIDLNNIKKINLYAKKITNLVKTKDFLKKNYHSWLIKHI
>Mature_697_residues
MNYLFASTNFGCEELLEKELRALGAKNLKVVKGGIYYEGEDSILYNSLMWSRIASRIFLCIKKFTIKNSNDLYKNTYNIN
WTQILYLEKTFLVKFRGTNNIIRNSLFGALKIKDAIVDTFYQKYSARPNINLLTPDVRIISYLCQNLVHIMLDLSGEALN
KRGYRKFFNISPIKENLSAAIILSSGWKKNTPLIDPMCGSGTLLIEAAMISSDRAPGLKRTEWGFKSWKGHKEKVWKETL
KKAKERFKIGIKKCLKNYFIGYDCNPDVIKKAQKNVINANLENIISFFACNLSHLKNPYQKEEIGTLISNPPYGERCKTE
NNLIALYIELGIMSKKYFEKWNLSVFSSSEFLLNFLQMKSYKNFCLKNGPLYCTLKNYEIFLNKFNNTNKEYENRLEKNF
KKLKKWNDLKEIECFRVYDSDLPNYKLIVDVYKKWLVIQEYQAPKSINYEKAHKRLCSAIYHSKEILSIPTNNVVIKFRK
KQKRKEQYQKLFNSNSFFIIREYHVKLLVNLIDYLDTGLFSEHRLVRKLIGSMSKGKDFLNLFSYTGAASVYAGLGKSKS
ITTVDISNTYIQWSMRNMSINNLINSKNIFIQKDCLEWIISTKNKFDLIFINPPTFSNSKRMKKSFELKRDYIKLMINLK
QILRKDGNIIFSSSTHNFEIDLNNIKKINLYAKKITNLVKTKDFLKKNYHSWLIKHI

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=700, Percent_Identity=47.5714285714286, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=235, Percent_Identity=26.8085106382979, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_BUCAP (Q8K9I4)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660693.1
- ProteinModelPortal:   Q8K9I4
- SMR:   Q8K9I4
- EnsemblBacteria:   EBBUCT00000000219
- GeneID:   1005805
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg351
- GeneTree:   EBGT00050000007787
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- ProtClustDB:   PRK11783
- BioCyc:   BAPH198804:BUSG351-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 81676; Mature: 81676

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYLFASTNFGCEELLEKELRALGAKNLKVVKGGIYYEGEDSILYNSLMWSRIASRIFLC
CCEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHH
IKKFTIKNSNDLYKNTYNINWTQILYLEKTFLVKFRGTNNIIRNSLFGALKIKDAIVDTF
HHHHHCCCCCCCCCCEECCCEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
YQKYSARPNINLLTPDVRIISYLCQNLVHIMLDLSGEALNKRGYRKFFNISPIKENLSAA
HHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHCCCCCHHHCCCEE
IILSSGWKKNTPLIDPMCGSGTLLIEAAMISSDRAPGLKRTEWGFKSWKGHKEKVWKETL
EEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHCCCHHHHHHHHHH
KKAKERFKIGIKKCLKNYFIGYDCNPDVIKKAQKNVINANLENIISFFACNLSHLKNPYQ
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHCCCHH
KEEIGTLISNPPYGERCKTENNLIALYIELGIMSKKYFEKWNLSVFSSSEFLLNFLQMKS
HHHHHHHHCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHCCCEEECCHHHHHHHHHHHH
YKNFCLKNGPLYCTLKNYEIFLNKFNNTNKEYENRLEKNFKKLKKWNDLKEIECFRVYDS
HHHHHHCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHCEEEEEECC
DLPNYKLIVDVYKKWLVIQEYQAPKSINYEKAHKRLCSAIYHSKEILSIPTNNVVIKFRK
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEHH
KQKRKEQYQKLFNSNSFFIIREYHVKLLVNLIDYLDTGLFSEHRLVRKLIGSMSKGKDFL
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHCCHHHH
NLFSYTGAASVYAGLGKSKSITTVDISNTYIQWSMRNMSINNLINSKNIFIQKDCLEWII
HHHHHCCHHHHHHCCCCCCCEEEEECCCEEEEEEECCCCHHHHCCCCCEEEEHHHHHHHH
STKNKFDLIFINPPTFSNSKRMKKSFELKRDYIKLMINLKQILRKDGNIIFSSSTHNFEI
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEE
DLNNIKKINLYAKKITNLVKTKDFLKKNYHSWLIKHI
ECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNYLFASTNFGCEELLEKELRALGAKNLKVVKGGIYYEGEDSILYNSLMWSRIASRIFLC
CCEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHH
IKKFTIKNSNDLYKNTYNINWTQILYLEKTFLVKFRGTNNIIRNSLFGALKIKDAIVDTF
HHHHHCCCCCCCCCCEECCCEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
YQKYSARPNINLLTPDVRIISYLCQNLVHIMLDLSGEALNKRGYRKFFNISPIKENLSAA
HHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHCCCCCHHHCCCEE
IILSSGWKKNTPLIDPMCGSGTLLIEAAMISSDRAPGLKRTEWGFKSWKGHKEKVWKETL
EEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHCCCHHHHHHHHHH
KKAKERFKIGIKKCLKNYFIGYDCNPDVIKKAQKNVINANLENIISFFACNLSHLKNPYQ
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHCCCHH
KEEIGTLISNPPYGERCKTENNLIALYIELGIMSKKYFEKWNLSVFSSSEFLLNFLQMKS
HHHHHHHHCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHCCCEEECCHHHHHHHHHHHH
YKNFCLKNGPLYCTLKNYEIFLNKFNNTNKEYENRLEKNFKKLKKWNDLKEIECFRVYDS
HHHHHHCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHCEEEEEECC
DLPNYKLIVDVYKKWLVIQEYQAPKSINYEKAHKRLCSAIYHSKEILSIPTNNVVIKFRK
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEHH
KQKRKEQYQKLFNSNSFFIIREYHVKLLVNLIDYLDTGLFSEHRLVRKLIGSMSKGKDFL
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHCCHHHH
NLFSYTGAASVYAGLGKSKSITTVDISNTYIQWSMRNMSINNLINSKNIFIQKDCLEWII
HHHHHCCHHHHHHCCCCCCCEEEEECCCEEEEEEECCCCHHHHCCCCCEEEEHHHHHHHH
STKNKFDLIFINPPTFSNSKRMKKSFELKRDYIKLMINLKQILRKDGNIIFSSSTHNFEI
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEE
DLNNIKKINLYAKKITNLVKTKDFLKKNYHSWLIKHI
ECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438