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The map label for this gene is pyrD

Identifier: 21672625

GI number: 21672625

Start: 396171

End: 397181

Strand: Direct

Name: pyrD

Synonym: BUsg350

Alternate gene names: 21672625

Gene position: 396171-397181 (Clockwise)

Preceding gene: 21672621

Following gene: 21672626

Centisome position: 61.76

GC content: 24.04

Gene sequence:

>1011_bases
ATGTTTTACTATTTAATTCGTAAACTTTTATTTTTGATTGATCCTGAAAAAGCACATACATTAGTATTAATGTACTTGAA
TTCTAAAAAAATACAAATCTTAAGAAAAATTTTTATCAAACCGATTCCATTAAAACAAATAAAATGTATGGGATTAACTT
TTAATAATAAAATTGGTTTAGCTGCAGGGATGGATAAAAATGGGGATTATATAGATTCTTTATCCAAAATAGGATTTGGT
TTTATCGAAGTAGGAACAGTTACTCCCTTACCTCAATACGGAAATCCAAAACCTAGGATTTTTAGAGTGCCTTCAATAGA
GGGAATTATTAATAGAATGGGGTTTAATAATTTAGGAATAGATTATTTAGTTAATAATGTAAAAAAATCGAAATTTAAGG
GAATAATCGGTATAAACATAGGAAAAAACAAAGATACAAAAATTGAAGACGCCATAAAAGATTATTTAATTTGTATAGAA
AAAATATATTTTTATGCTAGTTATATTGCTATAAATATTTCATCTCCAAATACTATTAATTTAAGAAAACTACAATATGG
TATATTATTCGAAAATTTATTAAGAGATATAAAAAAAAAACAAAGCGAAATGCATTCTAAATATTCTAAATATGTACCAA
TAGTAATTAAAATTTCTCCAGACCTATCAAAAAAAGAATTAATTCATATTTCTAATAAATTAATACACTATAAAATAGAT
GGAGTTATTGCAACTAATACAACATTAGATCATTCATCTATACCTAAAATTAAAAACAACAAAGAAGAAGGAGGGTTAAG
TGGTTTACCTTTACAAAAAAAAAGTAATGATGTGATATCAATATTATATAAAAATCTAAAAAGAAAAATTCCTATTATCG
GAGTAGGCGGTATTAATTCTATAAATTCTGCTAGAGAAAAAATTATGTGTGGAGCTAATTTAATACAAGTATATTCTGGA
TTGATATATCACGGTCCAAATTTTATTAGAGAAATAATTAGAAATCTATGA

Upstream 100 bases:

>100_bases
AATCAAAAAATAAATAAAAATTTTTTTCTTTTAAAAGAATTCACACTTTTTTATAAAAAATATATATACAATTTTTAACT
ATTCTTAAAAAGGAATTATA

Downstream 100 bases:

>100_bases
ATTAAGTAAGAGTATGAAGATCAATTAACAATCTATTTTAAACAAAAATTACTGATTTTTTATATTAACAAATCTTTTTA
GATGTTAATTTTTTTAAAAA

Product: dihydroorotate dehydrogenase 2

Products: NA

Alternate protein names: DHOdehase; DHOD; DHODase; Dihydroorotate oxidase

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MFYYLIRKLLFLIDPEKAHTLVLMYLNSKKIQILRKIFIKPIPLKQIKCMGLTFNNKIGLAAGMDKNGDYIDSLSKIGFG
FIEVGTVTPLPQYGNPKPRIFRVPSIEGIINRMGFNNLGIDYLVNNVKKSKFKGIIGINIGKNKDTKIEDAIKDYLICIE
KIYFYASYIAINISSPNTINLRKLQYGILFENLLRDIKKKQSEMHSKYSKYVPIVIKISPDLSKKELIHISNKLIHYKID
GVIATNTTLDHSSIPKIKNNKEEGGLSGLPLQKKSNDVISILYKNLKRKIPIIGVGGINSINSAREKIMCGANLIQVYSG
LIYHGPNFIREIIRNL

Sequences:

>Translated_336_residues
MFYYLIRKLLFLIDPEKAHTLVLMYLNSKKIQILRKIFIKPIPLKQIKCMGLTFNNKIGLAAGMDKNGDYIDSLSKIGFG
FIEVGTVTPLPQYGNPKPRIFRVPSIEGIINRMGFNNLGIDYLVNNVKKSKFKGIIGINIGKNKDTKIEDAIKDYLICIE
KIYFYASYIAINISSPNTINLRKLQYGILFENLLRDIKKKQSEMHSKYSKYVPIVIKISPDLSKKELIHISNKLIHYKID
GVIATNTTLDHSSIPKIKNNKEEGGLSGLPLQKKSNDVISILYKNLKRKIPIIGVGGINSINSAREKIMCGANLIQVYSG
LIYHGPNFIREIIRNL
>Mature_336_residues
MFYYLIRKLLFLIDPEKAHTLVLMYLNSKKIQILRKIFIKPIPLKQIKCMGLTFNNKIGLAAGMDKNGDYIDSLSKIGFG
FIEVGTVTPLPQYGNPKPRIFRVPSIEGIINRMGFNNLGIDYLVNNVKKSKFKGIIGINIGKNKDTKIEDAIKDYLICIE
KIYFYASYIAINISSPNTINLRKLQYGILFENLLRDIKKKQSEMHSKYSKYVPIVIKISPDLSKKELIHISNKLIHYKID
GVIATNTTLDHSSIPKIKNNKEEGGLSGLPLQKKSNDVISILYKNLKRKIPIIGVGGINSINSAREKIMCGANLIQVYSG
LIYHGPNFIREIIRNL

Specific function: Pyrimidine biosynthesis; fourth step. [C]

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI45006951, Length=345, Percent_Identity=35.9420289855072, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1787177, Length=336, Percent_Identity=55.3571428571429, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17509475, Length=336, Percent_Identity=38.3928571428571, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI281361352, Length=288, Percent_Identity=43.0555555555556, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI17137316, Length=288, Percent_Identity=43.0555555555556, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRD_BUCAP (Q8K9I5)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660692.1
- ProteinModelPortal:   Q8K9I5
- SMR:   Q8K9I5
- EnsemblBacteria:   EBBUCT00000000322
- GeneID:   1005806
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg350
- GeneTree:   EBGT00050000007915
- HOGENOM:   HBG351027
- OMA:   SYVTVNI
- ProtClustDB:   PRK05286
- BioCyc:   BAPH198804:BUSG350-MONOMER
- HAMAP:   MF_00225
- InterPro:   IPR013785
- InterPro:   IPR012135
- InterPro:   IPR005719
- InterPro:   IPR001295
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000164
- TIGRFAMs:   TIGR01036

Pfam domain/function: PF01180 DHO_dh

EC number: =1.3.5.2

Molecular weight: Translated: 38102; Mature: 38102

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS00911 DHODEHASE_1; PS00912 DHODEHASE_2

Important sites: ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYYLIRKLLFLIDPEKAHTLVLMYLNSKKIQILRKIFIKPIPLKQIKCMGLTFNNKIGL
CHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCHHHEEEEEEEECCCCCE
AAGMDKNGDYIDSLSKIGFGFIEVGTVTPLPQYGNPKPRIFRVPSIEGIINRMGFNNLGI
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCCH
DYLVNNVKKSKFKGIIGINIGKNKDTKIEDAIKDYLICIEKIYFYASYIAINISSPNTIN
HHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEE
LRKLQYGILFENLLRDIKKKQSEMHSKYSKYVPIVIKISPDLSKKELIHISNKLIHYKID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCEEEEEEEC
GVIATNTTLDHSSIPKIKNNKEEGGLSGLPLQKKSNDVISILYKNLKRKIPIIGVGGINS
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCC
INSAREKIMCGANLIQVYSGLIYHGPNFIREIIRNL
HHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MFYYLIRKLLFLIDPEKAHTLVLMYLNSKKIQILRKIFIKPIPLKQIKCMGLTFNNKIGL
CHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCHHHEEEEEEEECCCCCE
AAGMDKNGDYIDSLSKIGFGFIEVGTVTPLPQYGNPKPRIFRVPSIEGIINRMGFNNLGI
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCCH
DYLVNNVKKSKFKGIIGINIGKNKDTKIEDAIKDYLICIEKIYFYASYIAINISSPNTIN
HHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEE
LRKLQYGILFENLLRDIKKKQSEMHSKYSKYVPIVIKISPDLSKKELIHISNKLIHYKID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCEEEEEEEC
GVIATNTTLDHSSIPKIKNNKEEGGLSGLPLQKKSNDVISILYKNLKRKIPIIGVGGINS
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCC
INSAREKIMCGANLIQVYSGLIYHGPNFIREIIRNL
HHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12089438