Click here to switch to the map view.

The map label for this gene is yciL

Identifier: 21672551

GI number: 21672551

Start: 307632

End: 308405

Strand: Direct

Name: yciL

Synonym: BUsg271

Alternate gene names: 21672551

Gene position: 307632-308405 (Clockwise)

Preceding gene: 21672550

Following gene: 21672552

Centisome position: 47.96

GC content: 24.16

Gene sequence:

>774_bases
ATGACTGCAAAAATACAAAAAATATTATCTGATTTAGGATATGGTTCACGTCGTTTTATTGAATGTATGATTAAATGCGG
TAAAATAAGTATTAATGGTGAAAAAGCTATTATTGGTCAGTATTTAAATAAAAAAAACCCTGGTGAAATTTTAATTGATA
AAAAAAAAATAATTGTAAAAAGAAACAAAAATCTTCCTAAAGTTTTAATTTATAATAAGCCTATAGGAGAAGTTTGCACT
AGAGATGATTTTCAAAAAAGATTAACTGTATTTGATAAACTTCCTAAATTGAATTTAAATCGATGGGTTAGTGTAGGAAG
ATTAGATATTAATACTAAAGGTTTGTTGTTATTCACTAATGACGGAACATTAGCTAATAAACTAATGCATCCACGTAGTC
AAATTGAAAGAGAATATAATATTCGAATTTTTGGAGAAATGAACAAAAATAAAATAAACATTTTAAGAAAAGGAGTTAAA
ATTATACATGGTTACGTTTCTTTTAAAGAAATAGTACCCTTGTACGATAAAAAAGAAGGCAAAAACAAATGGTTTAAGGG
TATTTTGTGCGAAGGTAAAAATCGTGAAATTAGATTAATGTTTAAATCTATTCAATGTCAAGTAAATCAATTAATTAGAG
TTCGATACGGTAATATTATCTTACCAAAAAATCTAAAAGAAGGTCAATGGATGATGTTAAATTCAATATTTTTGAAAAAA
TTATATAATCTAATTAATTTTGATAAAGAAATAATCAACAAGAAAAAAAATTGA

Upstream 100 bases:

>100_bases
TCTTATTGTATGAGTTATGGTTTATTATTCCGTCAGGATTAGGAATATTATTAACTTTAATTCCAATAAATTTTTTTAAA
AATATTATAAGGTTTTCAAA

Downstream 100 bases:

>100_bases
AAAAATTTTTATACACTACAAATTTATTTTTAAAAAGGTTAATTTTGTGAATTTACTTTTAAATTACGAGTTGTTTTTAG
CAAAAGCAATTACTTTTTTA

Product: hypothetical protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTAKIQKILSDLGYGSRRFIECMIKCGKISINGEKAIIGQYLNKKNPGEILIDKKKIIVKRNKNLPKVLIYNKPIGEVCT
RDDFQKRLTVFDKLPKLNLNRWVSVGRLDINTKGLLLFTNDGTLANKLMHPRSQIEREYNIRIFGEMNKNKINILRKGVK
IIHGYVSFKEIVPLYDKKEGKNKWFKGILCEGKNREIRLMFKSIQCQVNQLIRVRYGNIILPKNLKEGQWMMLNSIFLKK
LYNLINFDKEIINKKKN

Sequences:

>Translated_257_residues
MTAKIQKILSDLGYGSRRFIECMIKCGKISINGEKAIIGQYLNKKNPGEILIDKKKIIVKRNKNLPKVLIYNKPIGEVCT
RDDFQKRLTVFDKLPKLNLNRWVSVGRLDINTKGLLLFTNDGTLANKLMHPRSQIEREYNIRIFGEMNKNKINILRKGVK
IIHGYVSFKEIVPLYDKKEGKNKWFKGILCEGKNREIRLMFKSIQCQVNQLIRVRYGNIILPKNLKEGQWMMLNSIFLKK
LYNLINFDKEIINKKKN
>Mature_256_residues
TAKIQKILSDLGYGSRRFIECMIKCGKISINGEKAIIGQYLNKKNPGEILIDKKKIIVKRNKNLPKVLIYNKPIGEVCTR
DDFQKRLTVFDKLPKLNLNRWVSVGRLDINTKGLLLFTNDGTLANKLMHPRSQIEREYNIRIFGEMNKNKINILRKGVKI
IHGYVSFKEIVPLYDKKEGKNKWFKGILCEGKNREIRLMFKSIQCQVNQLIRVRYGNIILPKNLKEGQWMMLNSIFLKKL
YNLINFDKEIINKKKN

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787524, Length=256, Percent_Identity=45.3125, Blast_Score=229, Evalue=9e-62,
Organism=Escherichia coli, GI1790453, Length=247, Percent_Identity=27.5303643724696, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI87081838, Length=185, Percent_Identity=28.1081081081081, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788510, Length=174, Percent_Identity=27.0114942528736, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUB_BUCAP (P42395)

Other databases:

- EMBL:   Z19055
- EMBL:   AE013218
- PIR:   S36431
- RefSeq:   NP_660618.1
- ProteinModelPortal:   P42395
- SMR:   P42395
- EnsemblBacteria:   EBBUCT00000000527
- GeneID:   1005475
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg271
- GeneTree:   EBGT00050000007870
- HOGENOM:   HBG754478
- OMA:   VNGSIAK
- ProtClustDB:   CLSK315856
- BioCyc:   BAPH198804:BUSG271-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 30100; Mature: 29968

Theoretical pI: Translated: 10.78; Mature: 10.78

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKIQKILSDLGYGSRRFIECMIKCGKISINGEKAIIGQYLNKKNPGEILIDKKKIIVK
CCHHHHHHHHHCCCCHHHHHHHHHHHCEEEECCCHHHHHHHHCCCCCCCEEEECEEEEEE
RNKNLPKVLIYNKPIGEVCTRDDFQKRLTVFDKLPKLNLNRWVSVGRLDINTKGLLLFTN
CCCCCCEEEEECCCHHHHHCHHHHHHHHHHHHCCCCCCHHHHEEECEEEECCCEEEEEEC
DGTLANKLMHPRSQIEREYNIRIFGEMNKNKINILRKGVKIIHGYVSFKEIVPLYDKKEG
CCHHHHHHCCCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KNKWFKGILCEGKNREIRLMFKSIQCQVNQLIRVRYGNIILPKNLKEGQWMMLNSIFLKK
CCHHHEEEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHH
LYNLINFDKEIINKKKN
HHHHHCCCHHHHCCCCC
>Mature Secondary Structure 
TAKIQKILSDLGYGSRRFIECMIKCGKISINGEKAIIGQYLNKKNPGEILIDKKKIIVK
CHHHHHHHHHCCCCHHHHHHHHHHHCEEEECCCHHHHHHHHCCCCCCCEEEECEEEEEE
RNKNLPKVLIYNKPIGEVCTRDDFQKRLTVFDKLPKLNLNRWVSVGRLDINTKGLLLFTN
CCCCCCEEEEECCCHHHHHCHHHHHHHHHHHHCCCCCCHHHHEEECEEEECCCEEEEEEC
DGTLANKLMHPRSQIEREYNIRIFGEMNKNKINILRKGVKIIHGYVSFKEIVPLYDKKEG
CCHHHHHHCCCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KNKWFKGILCEGKNREIRLMFKSIQCQVNQLIRVRYGNIILPKNLKEGQWMMLNSIFLKK
CCHHHEEEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHH
LYNLINFDKEIINKKKN
HHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407819; 12089438