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The map label for this gene is lpdA

Identifier: 21672487

GI number: 21672487

Start: 228645

End: 230075

Strand: Direct

Name: lpdA

Synonym: BUsg201

Alternate gene names: 21672487

Gene position: 228645-230075 (Clockwise)

Preceding gene: 21672486

Following gene: 21672503

Centisome position: 35.64

GC content: 28.93

Gene sequence:

>1431_bases
ATGCATCAAGAAATTCAATCTGAAGTCGTAATTATAGGTTCAGGACCTGCAGGTTATTCTGCTGCCTTTCGATGTGCAGA
TCTAGGTTTAGAAACTGTTTTAATAGAACATCAAGAACGATTAGGTGGTGTTTGTTTGAATGTAGGATGCATTCCCTCAA
AATCATTATTGCATATAGCTAAGATAATAAAAGATGCAAGTGAATTATCTGAATCAGGAGTTTTTTTTAATAAACCAATT
ATTGATATTAAAAAGATTAATAATTGGAAAGAAAAAATTATTAAAAAACTTACTACTGGACTGTCTAATATGGGGGAGAA
AAGAAAAGTAAGAATCGTTCAAGGAAAAGCGTTATTTAATACTGATCATAGTGTTTTGGTAAAAAATAAAAAAAATGATT
TTACGATTTTTTTTAAACATGCAATTATTGCTACTGGCTCTAAACCTATAAAAATACCTTCCTTGCCTAATGAGGATAAT
AGAATTTGGAATTCAACAGATGCTCTATCATTAAAAAGCATACCTAATCGTTTTTTAATTATAGGAGGAGGTATTATTGG
ATTAGAAATGGCAACAATATATAGTGCATTAGGTTCAAAAGTAGATATTGTTGATCGATTTAATGCTTTTCTTCCTTCAG
TGGACAAAGATATCACTGATATATATATAAAAAGTATTAAAAAGAGATTTAAGTTATTATTAAATACTCATGTTAAAAGT
GTTGAAAAATCAAAAGATAACGATCTTATTGTAAAAATAGCTGAAGAAAACAGTGATGAAAATGTTTGTTGTTATGATAA
TATACTGGTTGCAATTGGAAGAAGTCCAAACGTTGATTTTTTAGGATTAGAAAAAATTGGATTAAAATTAAATGAATCTG
GATTTATTGAGATAAATCAACAATTAAAAACAAATATATCTCATATTTATGCTATTGGTGATGTCACAGGTTTTCCTATG
TTAGCTCACAAAGCAGTACAACAAGCACATATTGCAGCTGAAGTGATTTCTGGTAAAAAACATTATTTTGAGCCTAAAGT
TATTCCATCAGTTGCTTATACAGATCCTGAAATAGCTTGGGTAGGTTTAAGTGAAAAAGAAGCTGAAAATAATGATATAG
ATTATGAAGTGTCTTTATTTCCTTGGAGTGCATCAGGAAGAGCACATGCATCAAATTGTACTTTAGGTATGACAAAATTA
ATTTTTAATAAAAATACTAACAAGATTATTGGTGGATCTATAATAGGTACAAATGCAAGTGAATTAATCAGCGAAATTGG
ACTTGCAATTGAAATGGGATCTGATGCTGAAGATATTTCACTTACTATTCATCCACATCCAACTTTGAGTGAATCAATTT
CTTTGGCTTCAGAAGTTTTTCAAGGAACAATAACAGATCTTTTAAATTTAAAAAAATCGTTGTTAAATTAA

Upstream 100 bases:

>100_bases
ATGGAGCAGATGCCGCTCGTTTTTTAACTTTTATTGGAAAAATGTTATCTGATATACGTTTTTTAATTATGTGATTTTTT
TTCTAAAGAGGTTTATTAAA

Downstream 100 bases:

>100_bases
CAATTGATACACTTCTTTTCTTGGTGTAAAAGAAGTGTATCAATTTTTTAACTTTTGAATACATTATAAAAAATATAAGT
TCAAAAGTTATATTTTTTTA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MHQEIQSEVVIIGSGPAGYSAAFRCADLGLETVLIEHQERLGGVCLNVGCIPSKSLLHIAKIIKDASELSESGVFFNKPI
IDIKKINNWKEKIIKKLTTGLSNMGEKRKVRIVQGKALFNTDHSVLVKNKKNDFTIFFKHAIIATGSKPIKIPSLPNEDN
RIWNSTDALSLKSIPNRFLIIGGGIIGLEMATIYSALGSKVDIVDRFNAFLPSVDKDITDIYIKSIKKRFKLLLNTHVKS
VEKSKDNDLIVKIAEENSDENVCCYDNILVAIGRSPNVDFLGLEKIGLKLNESGFIEINQQLKTNISHIYAIGDVTGFPM
LAHKAVQQAHIAAEVISGKKHYFEPKVIPSVAYTDPEIAWVGLSEKEAENNDIDYEVSLFPWSASGRAHASNCTLGMTKL
IFNKNTNKIIGGSIIGTNASELISEIGLAIEMGSDAEDISLTIHPHPTLSESISLASEVFQGTITDLLNLKKSLLN

Sequences:

>Translated_476_residues
MHQEIQSEVVIIGSGPAGYSAAFRCADLGLETVLIEHQERLGGVCLNVGCIPSKSLLHIAKIIKDASELSESGVFFNKPI
IDIKKINNWKEKIIKKLTTGLSNMGEKRKVRIVQGKALFNTDHSVLVKNKKNDFTIFFKHAIIATGSKPIKIPSLPNEDN
RIWNSTDALSLKSIPNRFLIIGGGIIGLEMATIYSALGSKVDIVDRFNAFLPSVDKDITDIYIKSIKKRFKLLLNTHVKS
VEKSKDNDLIVKIAEENSDENVCCYDNILVAIGRSPNVDFLGLEKIGLKLNESGFIEINQQLKTNISHIYAIGDVTGFPM
LAHKAVQQAHIAAEVISGKKHYFEPKVIPSVAYTDPEIAWVGLSEKEAENNDIDYEVSLFPWSASGRAHASNCTLGMTKL
IFNKNTNKIIGGSIIGTNASELISEIGLAIEMGSDAEDISLTIHPHPTLSESISLASEVFQGTITDLLNLKKSLLN
>Mature_476_residues
MHQEIQSEVVIIGSGPAGYSAAFRCADLGLETVLIEHQERLGGVCLNVGCIPSKSLLHIAKIIKDASELSESGVFFNKPI
IDIKKINNWKEKIIKKLTTGLSNMGEKRKVRIVQGKALFNTDHSVLVKNKKNDFTIFFKHAIIATGSKPIKIPSLPNEDN
RIWNSTDALSLKSIPNRFLIIGGGIIGLEMATIYSALGSKVDIVDRFNAFLPSVDKDITDIYIKSIKKRFKLLLNTHVKS
VEKSKDNDLIVKIAEENSDENVCCYDNILVAIGRSPNVDFLGLEKIGLKLNESGFIEINQQLKTNISHIYAIGDVTGFPM
LAHKAVQQAHIAAEVISGKKHYFEPKVIPSVAYTDPEIAWVGLSEKEAENNDIDYEVSLFPWSASGRAHASNCTLGMTKL
IFNKNTNKIIGGSIIGTNASELISEIGLAIEMGSDAEDISLTIHPHPTLSESISLASEVFQGTITDLLNLKKSLLN

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI91199540, Length=466, Percent_Identity=37.7682403433476, Blast_Score=307, Evalue=2e-83,
Organism=Homo sapiens, GI50301238, Length=451, Percent_Identity=27.4944567627494, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI291045266, Length=442, Percent_Identity=29.185520361991, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI22035672, Length=460, Percent_Identity=27.3913043478261, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI33519430, Length=440, Percent_Identity=27.2727272727273, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI33519428, Length=440, Percent_Identity=27.2727272727273, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI33519426, Length=440, Percent_Identity=27.2727272727273, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI148277065, Length=440, Percent_Identity=27.2727272727273, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI148277071, Length=440, Percent_Identity=27.2727272727273, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI291045268, Length=430, Percent_Identity=26.9767441860465, Blast_Score=122, Evalue=8e-28,
Organism=Escherichia coli, GI1786307, Length=471, Percent_Identity=56.9002123142251, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI87082354, Length=471, Percent_Identity=29.0870488322718, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI87081717, Length=453, Percent_Identity=28.2560706401766, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789915, Length=442, Percent_Identity=31.2217194570136, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=36.1471861471861, Blast_Score=294, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI17557007, Length=478, Percent_Identity=26.9874476987448, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71983419, Length=447, Percent_Identity=29.082774049217, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=447, Percent_Identity=29.082774049217, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71982272, Length=445, Percent_Identity=26.0674157303371, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17559934, Length=241, Percent_Identity=26.9709543568465, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=463, Percent_Identity=39.3088552915767, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=30.2771855010661, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6325166, Length=453, Percent_Identity=28.2560706401766, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI21358499, Length=458, Percent_Identity=36.4628820960699, Blast_Score=298, Evalue=7e-81,
Organism=Drosophila melanogaster, GI24640549, Length=475, Percent_Identity=28, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=28, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24640553, Length=475, Percent_Identity=28, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI17737741, Length=480, Percent_Identity=23.75, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): DLDH_BUCAP (Q8K9T7)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660554.1
- ProteinModelPortal:   Q8K9T7
- SMR:   Q8K9T7
- EnsemblBacteria:   EBBUCT00000000211
- GeneID:   1005398
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg201
- GeneTree:   EBGT00050000008065
- HOGENOM:   HBG515043
- OMA:   MDANTIR
- ProtClustDB:   CLSK315791
- BioCyc:   BAPH198804:BUSG201-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327
- Gene3D:   G3DSA:3.30.390.30
- PANTHER:   PTHR22912:SF20
- PRINTS:   PR00368
- TIGRFAMs:   TIGR01350

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.8.1.4

Molecular weight: Translated: 52188; Mature: 52188

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: ACT_SITE 446-446 BINDING 54-54 BINDING 117-117 BINDING 205-205 BINDING 238-238 BINDING 314-314 BINDING 322-322

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQEIQSEVVIIGSGPAGYSAAFRCADLGLETVLIEHQERLGGVCLNVGCIPSKSLLHIA
CCCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHH
KIIKDASELSESGVFFNKPIIDIKKINNWKEKIIKKLTTGLSNMGEKRKVRIVQGKALFN
HHHHHHHHHHHCCCEECCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEC
TDHSVLVKNKKNDFTIFFKHAIIATGSKPIKIPSLPNEDNRIWNSTDALSLKSIPNRFLI
CCCEEEEECCCCCEEEEEEEEEEECCCCCEECCCCCCCCCCEECCCCCEECCCCCCEEEE
IGGGIIGLEMATIYSALGSKVDIVDRFNAFLPSVDKDITDIYIKSIKKRFKLLLNTHVKS
EECCHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VEKSKDNDLIVKIAEENSDENVCCYDNILVAIGRSPNVDFLGLEKIGLKLNESGFIEINQ
HHCCCCCCEEEEEECCCCCCCEEEECCEEEEECCCCCCCEEEHHHHCCEECCCCCEEECH
QLKTNISHIYAIGDVTGFPMLAHKAVQQAHIAAEVISGKKHYFEPKVIPSVAYTDPEIAW
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCEECCCCCEEE
VGLSEKEAENNDIDYEVSLFPWSASGRAHASNCTLGMTKLIFNKNTNKIIGGSIIGTNAS
EECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEEECCCCEEECCEEECCCHH
ELISEIGLAIEMGSDAEDISLTIHPHPTLSESISLASEVFQGTITDLLNLKKSLLN
HHHHHCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHQEIQSEVVIIGSGPAGYSAAFRCADLGLETVLIEHQERLGGVCLNVGCIPSKSLLHIA
CCCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHH
KIIKDASELSESGVFFNKPIIDIKKINNWKEKIIKKLTTGLSNMGEKRKVRIVQGKALFN
HHHHHHHHHHHCCCEECCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEC
TDHSVLVKNKKNDFTIFFKHAIIATGSKPIKIPSLPNEDNRIWNSTDALSLKSIPNRFLI
CCCEEEEECCCCCEEEEEEEEEEECCCCCEECCCCCCCCCCEECCCCCEECCCCCCEEEE
IGGGIIGLEMATIYSALGSKVDIVDRFNAFLPSVDKDITDIYIKSIKKRFKLLLNTHVKS
EECCHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VEKSKDNDLIVKIAEENSDENVCCYDNILVAIGRSPNVDFLGLEKIGLKLNESGFIEINQ
HHCCCCCCEEEEEECCCCCCCEEEECCEEEEECCCCCCCEEEHHHHCCEECCCCCEEECH
QLKTNISHIYAIGDVTGFPMLAHKAVQQAHIAAEVISGKKHYFEPKVIPSVAYTDPEIAW
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCEECCCCCEEE
VGLSEKEAENNDIDYEVSLFPWSASGRAHASNCTLGMTKLIFNKNTNKIIGGSIIGTNAS
EECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEEECCCCEEECCEEECCCHH
ELISEIGLAIEMGSDAEDISLTIHPHPTLSESISLASEVFQGTITDLLNLKKSLLN
HHHHHCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438