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The map label for this gene is ychF

Identifier: 21672472

GI number: 21672472

Start: 206655

End: 207743

Strand: Direct

Name: ychF

Synonym: BUsg185

Alternate gene names: 21672472

Gene position: 206655-207743 (Clockwise)

Preceding gene: 30410817

Following gene: 21672476

Centisome position: 32.22

GC content: 25.71

Gene sequence:

>1089_bases
ATGGGTTTTAAATGTGGAATTATAGGATTACCCAATGTGGGTAAATCTACTTTATTTAATGTTTTAACTAAAGGTAATTC
GGCAGTTGCCAATTTTCCATTTTGTACTATTAAGCCAAATATAGGTATTGTTTCAGTCCCTGATAATCGCATTAATAATC
TTTCTAAAATTATTTTACCTAAAAAAATAACAAATGCTTTTATAGAATTTGTAGATATTGCTGGTTTAGTGAAAGGTGCG
TCTAAAGGAGAAGGTTTAGGTAATCAATTTTTAAGTAACATACGTGATACTCATGCAATTGCTCATGTTGTTCGCTGTTT
CAAAGATGATAATGTTAGTCATATTTATAATCAACTACAACCTAAAATAGATGTTGATATAATTAATTCTGAACTTATAT
TAGCAGATTTTGAAACTTGTGAAAAATCTATATTAAAATTGCAAAAAAAACTAAATTTTAATAAAAAAGAGATAGAAGAA
AAATTAGTTGTATTAAGTAAGTGCCTTGATCATTTAAAAAAATTTTTAATGTTAAAAACACTTGAGTTAGATGAAAATGA
AAAGAAAATTATTAGTTATTTACGATTTTTAACTTTAAAACCCACAATGTATATTGCTAATATTAATGAACAAAAAGAGT
CATGTTTTTTTTTAAATGAGTTAGAAAAAATGGCTAAAAAAGAAGGTTCTTCAGTCATTCCAATTAATTCGAATCTAGAA
TTAGATTTAATAAAAATGAATGAAGATGAACAAAAATATTTTATGAAATCTTTTAATATAACGAATTTAGGATTAAATAA
TATTATTAAATCTGGTTATAAAATACTTAATTTAATAACATTTTTTACCGCCGGAATAAAAGAGGTTCGTGCTTGGGCTA
TACCTAATGGCAGTACGAGTCTTCAAGCTGCTGATAAAATACATAGTGATTTTAGACGAGGTTTTATTCGGGCACAAATC
ATCAATTATTTAGATTTTATAAAATATAAAAGTGAGACGAAGGTTAAAGAAGCAGGGAAGTATCGAAGCGAAGGAAAATT
TTATCAAATTCAAGATGGCGACATTATTAATTTTTTATTTAATGTTTAA

Upstream 100 bases:

>100_bases
TTTACATTATTAAATTTGATACAATAGTCAGATATAAAAATTTTTTTATATTTTTTAAATCAATTTTGTGTAATTGTTCA
TTTTTTAAGGCGTATAAAAT

Downstream 100 bases:

>100_bases
AAAAAGTTATTTTTTATTAGAGAGGAAAATTTAATTTATCCTCTCTAATTTATATGATTATTTTTCTAATAAAAATTCTT
TTAATTTATTAAAATCAGGA

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MGFKCGIIGLPNVGKSTLFNVLTKGNSAVANFPFCTIKPNIGIVSVPDNRINNLSKIILPKKITNAFIEFVDIAGLVKGA
SKGEGLGNQFLSNIRDTHAIAHVVRCFKDDNVSHIYNQLQPKIDVDIINSELILADFETCEKSILKLQKKLNFNKKEIEE
KLVVLSKCLDHLKKFLMLKTLELDENEKKIISYLRFLTLKPTMYIANINEQKESCFFLNELEKMAKKEGSSVIPINSNLE
LDLIKMNEDEQKYFMKSFNITNLGLNNIIKSGYKILNLITFFTAGIKEVRAWAIPNGSTSLQAADKIHSDFRRGFIRAQI
INYLDFIKYKSETKVKEAGKYRSEGKFYQIQDGDIINFLFNV

Sequences:

>Translated_362_residues
MGFKCGIIGLPNVGKSTLFNVLTKGNSAVANFPFCTIKPNIGIVSVPDNRINNLSKIILPKKITNAFIEFVDIAGLVKGA
SKGEGLGNQFLSNIRDTHAIAHVVRCFKDDNVSHIYNQLQPKIDVDIINSELILADFETCEKSILKLQKKLNFNKKEIEE
KLVVLSKCLDHLKKFLMLKTLELDENEKKIISYLRFLTLKPTMYIANINEQKESCFFLNELEKMAKKEGSSVIPINSNLE
LDLIKMNEDEQKYFMKSFNITNLGLNNIIKSGYKILNLITFFTAGIKEVRAWAIPNGSTSLQAADKIHSDFRRGFIRAQI
INYLDFIKYKSETKVKEAGKYRSEGKFYQIQDGDIINFLFNV
>Mature_361_residues
GFKCGIIGLPNVGKSTLFNVLTKGNSAVANFPFCTIKPNIGIVSVPDNRINNLSKIILPKKITNAFIEFVDIAGLVKGAS
KGEGLGNQFLSNIRDTHAIAHVVRCFKDDNVSHIYNQLQPKIDVDIINSELILADFETCEKSILKLQKKLNFNKKEIEEK
LVVLSKCLDHLKKFLMLKTLELDENEKKIISYLRFLTLKPTMYIANINEQKESCFFLNELEKMAKKEGSSVIPINSNLEL
DLIKMNEDEQKYFMKSFNITNLGLNNIIKSGYKILNLITFFTAGIKEVRAWAIPNGSTSLQAADKIHSDFRRGFIRAQII
NYLDFIKYKSETKVKEAGKYRSEGKFYQIQDGDIINFLFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI58761500, Length=370, Percent_Identity=41.6216216216216, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI58761502, Length=186, Percent_Identity=40.8602150537634, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI24308117, Length=192, Percent_Identity=28.125, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1787454, Length=363, Percent_Identity=56.198347107438, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1789574, Length=180, Percent_Identity=25.5555555555556, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17509631, Length=369, Percent_Identity=40.9214092140921, Blast_Score=274, Evalue=7e-74,
Organism=Saccharomyces cerevisiae, GI6319499, Length=372, Percent_Identity=37.3655913978495, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321773, Length=381, Percent_Identity=35.6955380577428, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321649, Length=115, Percent_Identity=39.1304347826087, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24640873, Length=370, Percent_Identity=38.9189189189189, Blast_Score=257, Evalue=9e-69,
Organism=Drosophila melanogaster, GI24640877, Length=370, Percent_Identity=38.9189189189189, Blast_Score=257, Evalue=9e-69,
Organism=Drosophila melanogaster, GI24640875, Length=370, Percent_Identity=38.9189189189189, Blast_Score=257, Evalue=9e-69,
Organism=Drosophila melanogaster, GI24640879, Length=329, Percent_Identity=36.1702127659575, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24585318, Length=152, Percent_Identity=32.2368421052632, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ENGD_BUCAP (Q8K9V2)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660539.1
- ProteinModelPortal:   Q8K9V2
- SMR:   Q8K9V2
- EnsemblBacteria:   EBBUCT00000000281
- GeneID:   1005382
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg185
- GeneTree:   EBGT00050000007775
- HOGENOM:   HBG586249
- OMA:   TANENEA
- ProtClustDB:   PRK09601
- BioCyc:   BAPH198804:BUSG185-MONOMER
- GO:   GO:0005622
- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192
- Gene3D:   G3DSA:3.10.20.30
- PANTHER:   PTHR23305:SF4
- PIRSF:   PIRSF006641
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00092

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C; SSF81271 TGS-like

EC number: NA

Molecular weight: Translated: 41125; Mature: 40993

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKCGIIGLPNVGKSTLFNVLTKGNSAVANFPFCTIKPNIGIVSVPDNRINNLSKIILP
CCEEECEEECCCCCHHHHHHHHHCCCCCEECCCEEEECCCCCEEECCCHHHHHHHHHHCC
KKITNAFIEFVDIAGLVKGASKGEGLGNQFLSNIRDTHAIAHVVRCFKDDNVSHIYNQLQ
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
PKIDVDIINSELILADFETCEKSILKLQKKLNFNKKEIEEKLVVLSKCLDHLKKFLMLKT
CCEEEEEECCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LELDENEKKIISYLRFLTLKPTMYIANINEQKESCFFLNELEKMAKKEGSSVIPINSNLE
HCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCE
LDLIKMNEDEQKYFMKSFNITNLGLNNIIKSGYKILNLITFFTAGIKEVRAWAIPNGSTS
EEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
LQAADKIHSDFRRGFIRAQIINYLDFIKYKSETKVKEAGKYRSEGKFYQIQDGDIINFLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEECCCCEEHHEE
NV
CC
>Mature Secondary Structure 
GFKCGIIGLPNVGKSTLFNVLTKGNSAVANFPFCTIKPNIGIVSVPDNRINNLSKIILP
CEEECEEECCCCCHHHHHHHHHCCCCCEECCCEEEECCCCCEEECCCHHHHHHHHHHCC
KKITNAFIEFVDIAGLVKGASKGEGLGNQFLSNIRDTHAIAHVVRCFKDDNVSHIYNQLQ
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
PKIDVDIINSELILADFETCEKSILKLQKKLNFNKKEIEEKLVVLSKCLDHLKKFLMLKT
CCEEEEEECCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LELDENEKKIISYLRFLTLKPTMYIANINEQKESCFFLNELEKMAKKEGSSVIPINSNLE
HCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCE
LDLIKMNEDEQKYFMKSFNITNLGLNNIIKSGYKILNLITFFTAGIKEVRAWAIPNGSTS
EEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
LQAADKIHSDFRRGFIRAQIINYLDFIKYKSETKVKEAGKYRSEGKFYQIQDGDIINFLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEECCCCEEHHEE
NV
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438