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The map label for this gene is prfA

Identifier: 21672454

GI number: 21672454

Start: 185288

End: 186373

Strand: Direct

Name: prfA

Synonym: BUsg165

Alternate gene names: 21672454

Gene position: 185288-186373 (Clockwise)

Preceding gene: 21672451

Following gene: 21672455

Centisome position: 28.89

GC content: 28.08

Gene sequence:

>1086_bases
ATGAATTCGTCTATTTTTAATAAATTAAAATCCTTAAGAAATCGTTATGAAGAAATTGAAATTATGCTTTCTCAAAAAGA
TACTATATCAAATCAGGAAAAATTTAAGAATCTATCTAAAGAATATTTACAAATTTCAGAAATTGTTAAAAATTTTATTA
AATGGGAAAAACTAGAAACTGATATTAAAAATATTAATTTATTATTTAATGATGTAGAAATGCATGACATAGCTCAAGAA
GAGCTTTTTATAGTAAAAAAACAAAAAAAAAAATTAGAAGAAAAAATTAAAATATTATTACTTCCTGTAGATCCTAATGA
TCAGCATAGTTGTTTTATTGAAATTAGAGCAGCAACAGGAGGAGACGAATCTTCAATTTTTGCTGGCGAATTATTTAGAA
TGTATACGCGTTATGCTGAAAATTATATGTGGAAAACAGAAATAATGAGTTCAAGTGAAAATGAAAGAGGAGGATTTAAA
GAAATAATAGCTAAAGTTACAGGAAAAGGTGCATGTGGTCGTTTAAAATTTGAATCTGGAGGGCATCGTGTTCAAAGAGT
ACCTGAAACAGAATCTCAAGGAAGAATTCATACATCAACTTGTACTGTAGCTATTATGCCTGTGAGACCTAAAACTAAAA
AAGAAGAAATTAAAGTTTCTGATTTAAAAATTGATACCTTTCGTTCCTCTGGTGCAGGTGGTCAACATGTAAATACTACT
GATTCTGCAATTCGCATCACTCATATTCCTAGTGGAAATGTAGTAGAATGTCAAGATGAAAGATCACAACATAAAAATAA
AGCTAAAGCTTTATCTATTTTATCTGCTCGTGTATACGCTGATAAATTAGCAAAAAGTCAAAAAGAAAATTCGTCTATGA
GACGTATTTTATTAGGAACTGGTGAAAGATCAGATAGAAATAGAACATATAATTTCGCTCAGAACAGAATTACAGATCAT
AGAATTAATCTTACTATATACAAATTAAATGAAGTTTTACAGGGAAAATTAGACTTACTTATTGATCCAATAATGCAAGA
GTATCAAGCAGATATGCTTTCTTCTTTATCTGAATTTAAATTATGA

Upstream 100 bases:

>100_bases
TTTTTATATTTGCATATGTATAATTAATGCTGTGAAATTTTTTATTCTTTTAACTTAACAAAGTTGTTTGTAAGATAAAT
TATAAATAGGATAATATTTT

Downstream 100 bases:

>100_bases
ATATAAACTATTGGTTAAAAAAAGCAATTAAAAAACTATCTCATTGTGAGAATCCTCGATATGAAGCTGAGATTTTATTA
AGTCATGTTTTAAAATGTAC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MNSSIFNKLKSLRNRYEEIEIMLSQKDTISNQEKFKNLSKEYLQISEIVKNFIKWEKLETDIKNINLLFNDVEMHDIAQE
ELFIVKKQKKKLEEKIKILLLPVDPNDQHSCFIEIRAATGGDESSIFAGELFRMYTRYAENYMWKTEIMSSSENERGGFK
EIIAKVTGKGACGRLKFESGGHRVQRVPETESQGRIHTSTCTVAIMPVRPKTKKEEIKVSDLKIDTFRSSGAGGQHVNTT
DSAIRITHIPSGNVVECQDERSQHKNKAKALSILSARVYADKLAKSQKENSSMRRILLGTGERSDRNRTYNFAQNRITDH
RINLTIYKLNEVLQGKLDLLIDPIMQEYQADMLSSLSEFKL

Sequences:

>Translated_361_residues
MNSSIFNKLKSLRNRYEEIEIMLSQKDTISNQEKFKNLSKEYLQISEIVKNFIKWEKLETDIKNINLLFNDVEMHDIAQE
ELFIVKKQKKKLEEKIKILLLPVDPNDQHSCFIEIRAATGGDESSIFAGELFRMYTRYAENYMWKTEIMSSSENERGGFK
EIIAKVTGKGACGRLKFESGGHRVQRVPETESQGRIHTSTCTVAIMPVRPKTKKEEIKVSDLKIDTFRSSGAGGQHVNTT
DSAIRITHIPSGNVVECQDERSQHKNKAKALSILSARVYADKLAKSQKENSSMRRILLGTGERSDRNRTYNFAQNRITDH
RINLTIYKLNEVLQGKLDLLIDPIMQEYQADMLSSLSEFKL
>Mature_361_residues
MNSSIFNKLKSLRNRYEEIEIMLSQKDTISNQEKFKNLSKEYLQISEIVKNFIKWEKLETDIKNINLLFNDVEMHDIAQE
ELFIVKKQKKKLEEKIKILLLPVDPNDQHSCFIEIRAATGGDESSIFAGELFRMYTRYAENYMWKTEIMSSSENERGGFK
EIIAKVTGKGACGRLKFESGGHRVQRVPETESQGRIHTSTCTVAIMPVRPKTKKEEIKVSDLKIDTFRSSGAGGQHVNTT
DSAIRITHIPSGNVVECQDERSQHKNKAKALSILSARVYADKLAKSQKENSSMRRILLGTGERSDRNRTYNFAQNRITDH
RINLTIYKLNEVLQGKLDLLIDPIMQEYQADMLSSLSEFKL

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=290, Percent_Identity=43.7931034482759, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI34577120, Length=363, Percent_Identity=34.7107438016529, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI166795305, Length=195, Percent_Identity=42.5641025641026, Blast_Score=165, Evalue=8e-41,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=60.8938547486033, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI2367172, Length=342, Percent_Identity=34.2105263157895, Blast_Score=187, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=306, Percent_Identity=30.718954248366, Blast_Score=164, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6321295, Length=324, Percent_Identity=39.8148148148148, Blast_Score=227, Evalue=2e-60,
Organism=Drosophila melanogaster, GI19921226, Length=364, Percent_Identity=35.7142857142857, Blast_Score=205, Evalue=4e-53,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_BUCAP (Q8K9X0)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660521.1
- ProteinModelPortal:   Q8K9X0
- SMR:   Q8K9X0
- EnsemblBacteria:   EBBUCT00000000434
- GeneID:   1005362
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg165
- GeneTree:   EBGT00050000007801
- HOGENOM:   HBG629764
- OMA:   NTNEGEH
- ProtClustDB:   PRK00591
- BioCyc:   BAPH198804:BUSG165-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41460; Mature: 41460

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSIFNKLKSLRNRYEEIEIMLSQKDTISNQEKFKNLSKEYLQISEIVKNFIKWEKLET
CCHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIKNINLLFNDVEMHDIAQEELFIVKKQKKKLEEKIKILLLPVDPNDQHSCFIEIRAATG
HHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCEEEEEEEECCC
GDESSIFAGELFRMYTRYAENYMWKTEIMSSSENERGGFKEIIAKVTGKGACGRLKFESG
CCCCCHHHHHHHHHHHHHHHCCEEHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEECCC
GHRVQRVPETESQGRIHTSTCTVAIMPVRPKTKKEEIKVSDLKIDTFRSSGAGGQHVNTT
CCCEEECCCCCCCCEEEEEEEEEEEEECCCCCCHHCEEEEEEEEEECCCCCCCCCCCCCC
DSAIRITHIPSGNVVECQDERSQHKNKAKALSILSARVYADKLAKSQKENSSMRRILLGT
CCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEC
GERSDRNRTYNFAQNRITDHRINLTIYKLNEVLQGKLDLLIDPIMQEYQADMLSSLSEFK
CCCCCCCCCHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
L
C
>Mature Secondary Structure
MNSSIFNKLKSLRNRYEEIEIMLSQKDTISNQEKFKNLSKEYLQISEIVKNFIKWEKLET
CCHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIKNINLLFNDVEMHDIAQEELFIVKKQKKKLEEKIKILLLPVDPNDQHSCFIEIRAATG
HHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCEEEEEEEECCC
GDESSIFAGELFRMYTRYAENYMWKTEIMSSSENERGGFKEIIAKVTGKGACGRLKFESG
CCCCCHHHHHHHHHHHHHHHCCEEHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEECCC
GHRVQRVPETESQGRIHTSTCTVAIMPVRPKTKKEEIKVSDLKIDTFRSSGAGGQHVNTT
CCCEEECCCCCCCCEEEEEEEEEEEEECCCCCCHHCEEEEEEEEEECCCCCCCCCCCCCC
DSAIRITHIPSGNVVECQDERSQHKNKAKALSILSARVYADKLAKSQKENSSMRRILLGT
CCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEC
GERSDRNRTYNFAQNRITDHRINLTIYKLNEVLQGKLDLLIDPIMQEYQADMLSSLSEFK
CCCCCCCCCHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438