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The map label for this gene is folC

Identifier: 21672451

GI number: 21672451

Start: 181237

End: 182493

Strand: Direct

Name: folC

Synonym: BUsg161

Alternate gene names: 21672451

Gene position: 181237-182493 (Clockwise)

Preceding gene: 21672450

Following gene: 21672454

Centisome position: 28.25

GC content: 26.09

Gene sequence:

>1257_bases
ATGCACAAAAAAAAATATACCTTTTCTATGTGGATGAAATATTTAGAAAAATTTGATAAAAAAGATAGAAAAAATCTTTT
TGAATTAAAACTTATTGCAAAAAAATTAGGTTTATTAAACTTAAAATCCTTTTTTTTTACTGTTGGTGGCACTAATGGAA
AAGGAACAACCTGTGCAATGTTAGAAAAATTACTTTTGGATTCAGGTTATCAAGTAGGATTATATACCTCCCCCCATCTT
ATAAATTATTCTGAAAGAATTAAAGTTAATGGATTATATCTTAGTGAAAAAGATCACATTTTTTCTTTTTTAATTATAGA
TGCTGAAAAAGGTAATGTTTCTTTAACTTACTTTGAATTTATTACTCTTTCAGCCTTATTTCTATTCAGTCAATATTCTT
TGGATATAATAATATTAGAAGTAGGTTTAGGAGGACGTTTAGATGCAACTAATATTATAGATTCAGACTTATCTGTTATC
ACTAATATAGGGATTGATCATACTTCTTGTTTAGGAACAGATCGAATTAGTATCGGTCGTGAAAAATCTGGGATTTTTAG
AAAAGGCAAAATTGCAGTTATTGGAGAAAAAAACATCCCAATTTCTGTTGACGAAATAGCTAAAGAAAAAAAAACAATTT
TAAAAAAAATTGATGTAGATTGGTTTTGGACAAGAACAAAAATTGATTCATGGAATTTTATTCATTCAAATATTGAATTA
TATAATCTTCCAGTTTCTCGAATACCTTTGTCTAACACTGCTATTGCATTAGCATCTTTATTCTATTCTGGTTTAGAAGT
TAATTTAAAAAAATTAAAAAGTAGTATTTCTAAAGTGCAATTATCTGGTAGATTTCAAACAGTTTTTAATTCTCCTCGAA
TCATTCTCGATGTTGCTCATAATGTTCATGCGGCCTTATATCTATCAGAAAAAATTGATGAAATAGATACAGAAGGACAA
ATATATGCAGTTTTTGGAATCTTAAAAGATAAAGATGTTGCAGGAGTTGTTCAAGTATTACAAAAAAAAATTAACTACTG
GTATGTTGTAAACTTAAAAACAAATCGTAGTGCTAGTATAAACTATTTAAAGAAAAAGTTATCCCTTAATAACGCTCTTT
TTTTTAATAATATTAATGAAAGTTGGCAAGCGATTAAAAAAGTAATAACTAAAAAAGATATTATTTTAGTCTTTGGTTCT
TTTTTTACTGTCTCTGAATTTATGTCTATAAAAGATAGAAGATTAACATTGTATTAA

Upstream 100 bases:

>100_bases
AAAAATATAAAATTTTTTATTAGTAGTGAAAAACATTATTTTTTATGTCATTTTTTATTTTTAGTAAATATATAAAAATT
TTTTTTAATAAATTGAAAAT

Downstream 100 bases:

>100_bases
AAATATAATTTTGGAAAAAAATATGATTTTTATAGATTATGTTTTTCTTTTAATTATTTTTAGTTCTTTTTTTTTAGGAT
TATTTCGTGGTTTTATTCAA

Product: dihydrofolate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MHKKKYTFSMWMKYLEKFDKKDRKNLFELKLIAKKLGLLNLKSFFFTVGGTNGKGTTCAMLEKLLLDSGYQVGLYTSPHL
INYSERIKVNGLYLSEKDHIFSFLIIDAEKGNVSLTYFEFITLSALFLFSQYSLDIIILEVGLGGRLDATNIIDSDLSVI
TNIGIDHTSCLGTDRISIGREKSGIFRKGKIAVIGEKNIPISVDEIAKEKKTILKKIDVDWFWTRTKIDSWNFIHSNIEL
YNLPVSRIPLSNTAIALASLFYSGLEVNLKKLKSSISKVQLSGRFQTVFNSPRIILDVAHNVHAALYLSEKIDEIDTEGQ
IYAVFGILKDKDVAGVVQVLQKKINYWYVVNLKTNRSASINYLKKKLSLNNALFFNNINESWQAIKKVITKKDIILVFGS
FFTVSEFMSIKDRRLTLY

Sequences:

>Translated_418_residues
MHKKKYTFSMWMKYLEKFDKKDRKNLFELKLIAKKLGLLNLKSFFFTVGGTNGKGTTCAMLEKLLLDSGYQVGLYTSPHL
INYSERIKVNGLYLSEKDHIFSFLIIDAEKGNVSLTYFEFITLSALFLFSQYSLDIIILEVGLGGRLDATNIIDSDLSVI
TNIGIDHTSCLGTDRISIGREKSGIFRKGKIAVIGEKNIPISVDEIAKEKKTILKKIDVDWFWTRTKIDSWNFIHSNIEL
YNLPVSRIPLSNTAIALASLFYSGLEVNLKKLKSSISKVQLSGRFQTVFNSPRIILDVAHNVHAALYLSEKIDEIDTEGQ
IYAVFGILKDKDVAGVVQVLQKKINYWYVVNLKTNRSASINYLKKKLSLNNALFFNNINESWQAIKKVITKKDIILVFGS
FFTVSEFMSIKDRRLTLY
>Mature_418_residues
MHKKKYTFSMWMKYLEKFDKKDRKNLFELKLIAKKLGLLNLKSFFFTVGGTNGKGTTCAMLEKLLLDSGYQVGLYTSPHL
INYSERIKVNGLYLSEKDHIFSFLIIDAEKGNVSLTYFEFITLSALFLFSQYSLDIIILEVGLGGRLDATNIIDSDLSVI
TNIGIDHTSCLGTDRISIGREKSGIFRKGKIAVIGEKNIPISVDEIAKEKKTILKKIDVDWFWTRTKIDSWNFIHSNIEL
YNLPVSRIPLSNTAIALASLFYSGLEVNLKKLKSSISKVQLSGRFQTVFNSPRIILDVAHNVHAALYLSEKIDEIDTEGQ
IYAVFGILKDKDVAGVVQVLQKKINYWYVVNLKTNRSASINYLKKKLSLNNALFFNNINESWQAIKKVITKKDIILVFGS
FFTVSEFMSIKDRRLTLY

Specific function: Conversion of folates to polyglutamate derivatives

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family

Homologues:

Organism=Homo sapiens, GI66932984, Length=198, Percent_Identity=34.3434343434343, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI66932990, Length=198, Percent_Identity=34.3434343434343, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI1788654, Length=409, Percent_Identity=45.4767726161369, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71984923, Length=171, Percent_Identity=37.4269005847953, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17553150, Length=171, Percent_Identity=37.4269005847953, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17553148, Length=171, Percent_Identity=37.4269005847953, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323760, Length=320, Percent_Identity=28.4375, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324815, Length=232, Percent_Identity=33.1896551724138, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24641571, Length=400, Percent_Identity=25.5, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24581568, Length=143, Percent_Identity=35.6643356643357, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLC_BUCAP (Q8K9X3)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660518.1
- ProteinModelPortal:   Q8K9X3
- SMR:   Q8K9X3
- EnsemblBacteria:   EBBUCT00000000088
- GeneID:   1005359
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg161
- GeneTree:   EBGT00050000008195
- HOGENOM:   HBG744947
- OMA:   SIHPTEI
- ProtClustDB:   CLSK315624
- BioCyc:   BAPH198804:BUSG161-MONOMER
- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- PANTHER:   PTHR11136
- TIGRFAMs:   TIGR01499

Pfam domain/function: PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.17; =6.3.2.12

Molecular weight: Translated: 47631; Mature: 47631

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKKKYTFSMWMKYLEKFDKKDRKNLFELKLIAKKLGLLNLKSFFFTVGGTNGKGTTCAM
CCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHH
LEKLLLDSGYQVGLYTSPHLINYSERIKVNGLYLSEKDHIFSFLIIDAEKGNVSLTYFEF
HHHHHHCCCCEEEEEECCCEECCCCEEEEEEEEECCCCCEEEEEEEEECCCCEEEEHHHH
ITLSALFLFSQYSLDIIILEVGLGGRLDATNIIDSDLSVITNIGIDHTSCLGTDRISIGR
HHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHCCCHHHHHHCCCCCCCCCCCCCCCCCC
EKSGIFRKGKIAVIGEKNIPISVDEIAKEKKTILKKIDVDWFWTRTKIDSWNFIHSNIEL
CCCCCEECCCEEEEECCCCCEEHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCEEE
YNLPVSRIPLSNTAIALASLFYSGLEVNLKKLKSSISKVQLSGRFQTVFNSPRIILDVAH
EECCHHHCCCCHHHHHHHHHHHCCHHEEHHHHHHHHHHEEECCCEEEHHCCCEEEEEECC
NVHAALYLSEKIDEIDTEGQIYAVFGILKDKDVAGVVQVLQKKINYWYVVNLKTNRSASI
CCEEEEEEHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCEEEEEEEECCCCCHH
NYLKKKLSLNNALFFNNINESWQAIKKVITKKDIILVFGSFFTVSEFMSIKDRRLTLY
HHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MHKKKYTFSMWMKYLEKFDKKDRKNLFELKLIAKKLGLLNLKSFFFTVGGTNGKGTTCAM
CCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHH
LEKLLLDSGYQVGLYTSPHLINYSERIKVNGLYLSEKDHIFSFLIIDAEKGNVSLTYFEF
HHHHHHCCCCEEEEEECCCEECCCCEEEEEEEEECCCCCEEEEEEEEECCCCEEEEHHHH
ITLSALFLFSQYSLDIIILEVGLGGRLDATNIIDSDLSVITNIGIDHTSCLGTDRISIGR
HHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHCCCHHHHHHCCCCCCCCCCCCCCCCCC
EKSGIFRKGKIAVIGEKNIPISVDEIAKEKKTILKKIDVDWFWTRTKIDSWNFIHSNIEL
CCCCCEECCCEEEEECCCCCEEHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCEEE
YNLPVSRIPLSNTAIALASLFYSGLEVNLKKLKSSISKVQLSGRFQTVFNSPRIILDVAH
EECCHHHCCCCHHHHHHHHHHHCCHHEEHHHHHHHHHHEEECCCEEEHHCCCEEEEEECC
NVHAALYLSEKIDEIDTEGQIYAVFGILKDKDVAGVVQVLQKKINYWYVVNLKTNRSASI
CCEEEEEEHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCEEEEEEEECCCCCHH
NYLKKKLSLNNALFFNNINESWQAIKKVITKKDIILVFGSFFTVSEFMSIKDRRLTLY
HHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438