Click here to switch to the map view.

The map label for this gene is hemK

Identifier: 21672455

GI number: 21672455

Start: 186370

End: 187197

Strand: Direct

Name: hemK

Synonym: BUsg166

Alternate gene names: 21672455

Gene position: 186370-187197 (Clockwise)

Preceding gene: 21672454

Following gene: 21672456

Centisome position: 29.05

GC content: 24.76

Gene sequence:

>828_bases
ATGAATATAAACTATTGGTTAAAAAAAGCAATTAAAAAACTATCTCATTGTGAGAATCCTCGATATGAAGCTGAGATTTT
ATTAAGTCATGTTTTAAAATGTACACGTATTGCAATTATAATTAATCAAGAAATTGATTTAAGTAAAGAGCAATATCAAA
AATTAAACAATTTTATTTATAGAAGATCTATAGGTGAACCTATAGCCTATATAATAGGAAAAAAAGAATTTTGGTCTTTA
TCTCTTTGTGTGTCGTATAAAACACTTATTCCTAGACCAGATACTGAAATTTTAGTAGAAAAAATACTGTCTAAAGTTAA
TAAAAATTTCAGATCAATCCTTGATTTAGGAACAGGTTCCGGAGCGATCGCTTTAGCTTTAGCTAGTGTTTGTTCTCATT
GGAATATTATTGGTGTTGATAATTCATATTCAGCACTTAAAATAGCTAAGATAAACGGATTGAAATTAAATTTAAAAAAT
GTAGAATTTTTTTATAGTAACTGGTTTTCTCATATAAATGAGAAATTTCATATTATTGTCAGTAATCCTCCTTATATCGG
TATAAAAGAAATACAGTCACTTAAAAAAGATATTTTTTATGAACCATTTAATGCATTAATTTCAAAAAAAGATGGATTAC
TAGATATTGAATTAATTATACAAAAAGCAAGTCAATATTTATTTGACAAGGGTTGGTTATTTATTGAACATGGATGGAAA
CAAAAATTAAAAGTGCAGTATTTTTTTAAAAAATATAATTTTTTTTGTATACAATCTTTTAAAGATTATGGAGGTAATGA
TCGAATTACTTTTGGTCAAAAAAAATGA

Upstream 100 bases:

>100_bases
AATTAAATGAAGTTTTACAGGGAAAATTAGACTTACTTATTGATCCAATAATGCAAGAGTATCAAGCAGATATGCTTTCT
TCTTTATCTGAATTTAAATT

Downstream 100 bases:

>100_bases
TAAAATCATTAAAATACCATATTGAATAAAATAATTTAATATTTTTTTATATAAAAAAAATAAAAAATATAATTTTACAT
TATGATTAAATATATATTAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MNINYWLKKAIKKLSHCENPRYEAEILLSHVLKCTRIAIIINQEIDLSKEQYQKLNNFIYRRSIGEPIAYIIGKKEFWSL
SLCVSYKTLIPRPDTEILVEKILSKVNKNFRSILDLGTGSGAIALALASVCSHWNIIGVDNSYSALKIAKINGLKLNLKN
VEFFYSNWFSHINEKFHIIVSNPPYIGIKEIQSLKKDIFYEPFNALISKKDGLLDIELIIQKASQYLFDKGWLFIEHGWK
QKLKVQYFFKKYNFFCIQSFKDYGGNDRITFGQKK

Sequences:

>Translated_275_residues
MNINYWLKKAIKKLSHCENPRYEAEILLSHVLKCTRIAIIINQEIDLSKEQYQKLNNFIYRRSIGEPIAYIIGKKEFWSL
SLCVSYKTLIPRPDTEILVEKILSKVNKNFRSILDLGTGSGAIALALASVCSHWNIIGVDNSYSALKIAKINGLKLNLKN
VEFFYSNWFSHINEKFHIIVSNPPYIGIKEIQSLKKDIFYEPFNALISKKDGLLDIELIIQKASQYLFDKGWLFIEHGWK
QKLKVQYFFKKYNFFCIQSFKDYGGNDRITFGQKK
>Mature_275_residues
MNINYWLKKAIKKLSHCENPRYEAEILLSHVLKCTRIAIIINQEIDLSKEQYQKLNNFIYRRSIGEPIAYIIGKKEFWSL
SLCVSYKTLIPRPDTEILVEKILSKVNKNFRSILDLGTGSGAIALALASVCSHWNIIGVDNSYSALKIAKINGLKLNLKN
VEFFYSNWFSHINEKFHIIVSNPPYIGIKEIQSLKKDIFYEPFNALISKKDGLLDIELIIQKASQYLFDKGWLFIEHGWK
QKLKVQYFFKKYNFFCIQSFKDYGGNDRITFGQKK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family

Homologues:

Organism=Homo sapiens, GI7705409, Length=260, Percent_Identity=29.6153846153846, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1787463, Length=274, Percent_Identity=43.4306569343066, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI87082085, Length=194, Percent_Identity=32.4742268041237, Blast_Score=89, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24582226, Length=250, Percent_Identity=27.2, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEMK_BUCAP (Q8K9W9)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660522.1
- ProteinModelPortal:   Q8K9W9
- SMR:   Q8K9W9
- EnsemblBacteria:   EBBUCT00000000337
- GeneID:   1005363
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg166
- GeneTree:   EBGT00050000007761
- HOGENOM:   HBG732041
- OMA:   DPRPETE
- ProtClustDB:   PRK09328
- BioCyc:   BAPH198804:BUSG166-MONOMER
- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- TIGRFAMs:   TIGR00536
- TIGRFAMs:   TIGR03534

Pfam domain/function: PF05175 MTS

EC number: 2.1.1.-

Molecular weight: Translated: 32052; Mature: 32052

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS00092 N6_MTASE; PS00107 PROTEIN_KINASE_ATP

Important sites: BINDING 140-140 BINDING 168-168 BINDING 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNINYWLKKAIKKLSHCENPRYEAEILLSHVLKCTRIAIIINQEIDLSKEQYQKLNNFIY
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHH
RRSIGEPIAYIIGKKEFWSLSLCVSYKTLIPRPDTEILVEKILSKVNKNFRSILDLGTGS
HHHCCCCHHHHCCCCHHEEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GAIALALASVCSHWNIIGVDNSYSALKIAKINGLKLNLKNVEFFYSNWFSHINEKFHIIV
CHHHHHHHHHHHCCEEEECCCCCCEEEEEEECCEEEEECHHHHHHHHHHHHCCCEEEEEE
SNPPYIGIKEIQSLKKDIFYEPFNALISKKDGLLDIELIIQKASQYLFDKGWLFIEHGWK
CCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCC
QKLKVQYFFKKYNFFCIQSFKDYGGNDRITFGQKK
HHHHHHHHHHHHCCEEEEEHHHCCCCCCEECCCCC
>Mature Secondary Structure
MNINYWLKKAIKKLSHCENPRYEAEILLSHVLKCTRIAIIINQEIDLSKEQYQKLNNFIY
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHH
RRSIGEPIAYIIGKKEFWSLSLCVSYKTLIPRPDTEILVEKILSKVNKNFRSILDLGTGS
HHHCCCCHHHHCCCCHHEEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GAIALALASVCSHWNIIGVDNSYSALKIAKINGLKLNLKNVEFFYSNWFSHINEKFHIIV
CHHHHHHHHHHHCCEEEECCCCCCEEEEEEECCEEEEECHHHHHHHHHHHHCCCEEEEEE
SNPPYIGIKEIQSLKKDIFYEPFNALISKKDGLLDIELIIQKASQYLFDKGWLFIEHGWK
CCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCC
QKLKVQYFFKKYNFFCIQSFKDYGGNDRITFGQKK
HHHHHHHHHHHHCCEEEEEHHHCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438