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The map label for this gene is ksgA

Identifier: 21672424

GI number: 21672424

Start: 148228

End: 149052

Strand: Direct

Name: ksgA

Synonym: BUsg134

Alternate gene names: 21672424

Gene position: 148228-149052 (Clockwise)

Preceding gene: 21672423

Following gene: 21672425

Centisome position: 23.11

GC content: 21.58

Gene sequence:

>825_bases
ATGAAAAAAAAAATAAAAAAACATTTGCCTCTTAAAAGATTTAGTCAAAATTTTCTTATAAATCAAAATTTAATTAAAAA
AATAGTTAAGTTTATAAATCCTCAATTAAAGCAAACACTAGTTGAAATTGGTCCAGGTTTAGGTGCATTAACTAAACCTA
TATGCAATATAGTAGATGAGTTGATTGTTATTGAAATTGATTTAAATTTATTAAATTTTTTAAAAAAATATTCTTTTTAT
TCAAAGTTAATAGTTTTTTGTCAAGATGCTTTAATCTTTGATTATTTAAATTTGTTTTATAAAAAGAATAAATTAATTCG
CATTTTTGGAAATTTACCATATCATATTTCTACATCTTTGCTTTTTTGTTTTTTTGAAAAAAATAAAATAATTCAAGATA
TGAATTTTATGCTACAAAAAGAGGTTGCTGAACGTTTAATTGCTTTTCCGGGAACTAAATCATATGGTCGTTTAAGTATT
ATAGCGCAGTATTATTGTAATATTAAAATCATATTTAACGTTGCATCTGAAAATTTTCGACCTATTCCTAAGATTGATTC
TACATTTGTCAATTTAGTTCCTCATAAAAAATCTCCTTATTTTACTCATGATATCAAAGTTCTTAGTTATATAACAAATT
TAGCTTTTCAAAAAAGAAGAAAAATATTGCGTCATAGTTTAGGGAAGATATTTTCTGAAAAAATTTTTTTAAAATTAAAT
GTTGATCCAAAATTAAGAGCAGAAAATCTTTCTATACTGCAGTATTGCCAACTATCTAATTATATAATTGAAAATGATAT
TTTAAAAAATAAAAATTTTAATTAA

Upstream 100 bases:

>100_bases
AAATCATCTTATATTAAGATTTTTAAAAATTAATTAAATTTAAAATTAACTTTTTAGTAAAAAAAGTTTAATTCTTATAT
CAAGTAATTACTATATTCTA

Downstream 100 bases:

>100_bases
TTATTTTCTTTTAAAAAAGTGAAAAATATATGAGCATTTATTTTATCAGTGATATTCATGGTTGTTATAAAGAATTCAAG
TTACTTTTAAAAAAATCTTT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKKKIKKHLPLKRFSQNFLINQNLIKKIVKFINPQLKQTLVEIGPGLGALTKPICNIVDELIVIEIDLNLLNFLKKYSFY
SKLIVFCQDALIFDYLNLFYKKNKLIRIFGNLPYHISTSLLFCFFEKNKIIQDMNFMLQKEVAERLIAFPGTKSYGRLSI
IAQYYCNIKIIFNVASENFRPIPKIDSTFVNLVPHKKSPYFTHDIKVLSYITNLAFQKRRKILRHSLGKIFSEKIFLKLN
VDPKLRAENLSILQYCQLSNYIIENDILKNKNFN

Sequences:

>Translated_274_residues
MKKKIKKHLPLKRFSQNFLINQNLIKKIVKFINPQLKQTLVEIGPGLGALTKPICNIVDELIVIEIDLNLLNFLKKYSFY
SKLIVFCQDALIFDYLNLFYKKNKLIRIFGNLPYHISTSLLFCFFEKNKIIQDMNFMLQKEVAERLIAFPGTKSYGRLSI
IAQYYCNIKIIFNVASENFRPIPKIDSTFVNLVPHKKSPYFTHDIKVLSYITNLAFQKRRKILRHSLGKIFSEKIFLKLN
VDPKLRAENLSILQYCQLSNYIIENDILKNKNFN
>Mature_274_residues
MKKKIKKHLPLKRFSQNFLINQNLIKKIVKFINPQLKQTLVEIGPGLGALTKPICNIVDELIVIEIDLNLLNFLKKYSFY
SKLIVFCQDALIFDYLNLFYKKNKLIRIFGNLPYHISTSLLFCFFEKNKIIQDMNFMLQKEVAERLIAFPGTKSYGRLSI
IAQYYCNIKIIFNVASENFRPIPKIDSTFVNLVPHKKSPYFTHDIKVLSYITNLAFQKRRKILRHSLGKIFSEKIFLKLN
VDPKLRAENLSILQYCQLSNYIIENDILKNKNFN

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=277, Percent_Identity=32.8519855595668, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI7657198, Length=189, Percent_Identity=31.2169312169312, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1786236, Length=265, Percent_Identity=49.4339622641509, Blast_Score=280, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI25141369, Length=281, Percent_Identity=26.3345195729537, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25146882, Length=189, Percent_Identity=30.1587301587302, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324989, Length=199, Percent_Identity=29.6482412060301, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357273, Length=279, Percent_Identity=27.9569892473118, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21358017, Length=212, Percent_Identity=32.5471698113208, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_BUCAP (Q8KA00)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660491.1
- ProteinModelPortal:   Q8KA00
- SMR:   Q8KA00
- EnsemblBacteria:   EBBUCT00000000469
- GeneID:   1005951
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg134
- GeneTree:   EBGT00050000007888
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- ProtClustDB:   PRK00274
- BioCyc:   BAPH198804:BUSG134-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 32223; Mature: 32223

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 17-17 BINDING 19-19 BINDING 44-44 BINDING 65-65 BINDING 89-89 BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKIKKHLPLKRFSQNFLINQNLIKKIVKFINPQLKQTLVEIGPGLGALTKPICNIVDE
CCCCHHHCCCHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHH
LIVIEIDLNLLNFLKKYSFYSKLIVFCQDALIFDYLNLFYKKNKLIRIFGNLPYHISTSL
HEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
LFCFFEKNKIIQDMNFMLQKEVAERLIAFPGTKSYGRLSIIAQYYCNIKIIFNVASENFR
HEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCC
PIPKIDSTFVNLVPHKKSPYFTHDIKVLSYITNLAFQKRRKILRHSLGKIFSEKIFLKLN
CCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
VDPKLRAENLSILQYCQLSNYIIENDILKNKNFN
CCCCCCCCCHHHHHHHHHHCHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKKIKKHLPLKRFSQNFLINQNLIKKIVKFINPQLKQTLVEIGPGLGALTKPICNIVDE
CCCCHHHCCCHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHH
LIVIEIDLNLLNFLKKYSFYSKLIVFCQDALIFDYLNLFYKKNKLIRIFGNLPYHISTSL
HEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
LFCFFEKNKIIQDMNFMLQKEVAERLIAFPGTKSYGRLSIIAQYYCNIKIIFNVASENFR
HEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCC
PIPKIDSTFVNLVPHKKSPYFTHDIKVLSYITNLAFQKRRKILRHSLGKIFSEKIFLKLN
CCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
VDPKLRAENLSILQYCQLSNYIIENDILKNKNFN
CCCCCCCCCHHHHHHHHHHCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438