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The map label for this gene is murE

Identifier: 21241545

GI number: 21241545

Start: 918427

End: 919914

Strand: Direct

Name: murE

Synonym: XAC0775

Alternate gene names: 21241545

Gene position: 918427-919914 (Clockwise)

Preceding gene: 21241544

Following gene: 21241546

Centisome position: 17.75

GC content: 68.01

Gene sequence:

>1488_bases
GTGAGCCGCTCCATGGCGCTCTCGCAGTTGCTGCCGGACGTGGCGTTGACGCACGACGTGCAGGTGTCCGGGCTGGTGAT
GGATAGCCGTGCCGTGCGTCCGGGCGATGCCTTCGTGGCAATCGCCGGTTTCGGTGCGCATGGTCTGGGCTTTGTCGAAC
AGGCGCGCGCCAATGGCGCAGCGGCGGTGTTGTTCGAGCCGCCGGCTCCCGATGGACTGCCGGTCCCGGCCGATGCGATT
GCCGTGCCCGGTTTGCGTGCGCGCCTCGGCGTGATGGCCGATCAATTCCATGGCCGGCCGTCGCAGGCGATGCGTATGGT
CGGCGTCACCGGTACCAACGGCAAGACCTCCACCGTGCAGTTGCTGGCGCAGGCATTGACGCTGCTCGGTACGCCCACCG
GCACGCTTGGCACCTTGGGCGTCGGTCTGTATGGCGCGGCCGTGCCGACCGGTTTCACCACGCCGCTGGTGCTGCCCACG
CATGCCTTGCTGGCGCAGTTGCGCGATGACGGTGCGCAGGCCGTGGCAATGGAAGTCAGCTCGCACGCGCTCGATCAGGG
CCGCGTGGATGCGGTGCATTTCGACGTGGCGGTGTTCACCAATCTCACCCGCGACCATCTCGATTACCACGGCGACATGG
CGCAGTACGGTGCGGCGAAGGCCAAGCTGTTCGCCCGCGCGGGGTTGAAAGCGGCCGTGGTGAACCTGGACGATGCGTTC
GGCCACACACTGTTCGCTGCGCTCGATCCCGCGCTGCGCGCGATCGGTGTGAGCTCGCGCGCGCATGCCGACGCCAGCGT
GAGCGCGCAGGCACTGCAGCTGGACCACAACGGCATCAATTTCGCGCTCAATATCCGGGGCCAGGTGCATCCGGTGCATT
CGCCGCTGCTGGGCCGTTTCAACGTGGACAACCTGCTGGCCGTGGCCGGCGCGTTGTGGGCGATGGAGTTTGCGCCTGCG
CAGATCGCCAGCGTGCTCGGCCAGTTGCAGCCCATCCATGGCCGCATGAACCGCCTTGGTGGCGCACATGGCGCACCGTT
GGTGGTGGTCGATTACGCACACACGCCCGATGCGCTGGAGCAGGCATTGAGCAGCCTGCGCTCGCATGCGCAGGACCGCC
TGATCTGCGTGTTCGGCTGCGGTGGCGAGCGCGACACCGGCAAGCGCCCGCAGATGGCGGCGATCGCCGAGCTCAAGGCC
GACGTGGCGATCGTGACCGACGACAACCCGCGGGGCGAGGACGGCGATGCGATCGTGGCCGATATCCTGCGCGGCTTCGC
GCGACCGGATGCGGCCATCGTGCAGCGCGACCGCGCTGCTGCGATCCATCAGGCCATCGGCATGGCCAGCCCATCGGACA
TCGTGTTGATCGCCGGCAAGGGCCACGAGCCGTATCAGGAAGTTGCCGGCGTGCGGCATGCCTTCGACGACGCCCTCGTG
GCGGCGCAGGCGCTGCTGCCACGGACCGTATTGGGAGTGCGCCCATGA

Upstream 100 bases:

>100_bases
CGGACCCGGTGGATGAAGTCTCTGCCGGCATCCCGACCGCACTGGCGCCTGCGCCGGCTGCCGCACCAGCGCAGCCCGCG
CCGTTGCAGGAGAGCCGCCA

Downstream 100 bases:

>100_bases
AGCTCACGCCCCTGTCGTTGATTGCCCATTGGGCCGGCGGCGAGCTGCACGGCGACGACGCCATGATCGATGCGGTCGGC
AACGACACCCGCACGCTCGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSRSMALSQLLPDVALTHDVQVSGLVMDSRAVRPGDAFVAIAGFGAHGLGFVEQARANGAAAVLFEPPAPDGLPVPADAI
AVPGLRARLGVMADQFHGRPSQAMRMVGVTGTNGKTSTVQLLAQALTLLGTPTGTLGTLGVGLYGAAVPTGFTTPLVLPT
HALLAQLRDDGAQAVAMEVSSHALDQGRVDAVHFDVAVFTNLTRDHLDYHGDMAQYGAAKAKLFARAGLKAAVVNLDDAF
GHTLFAALDPALRAIGVSSRAHADASVSAQALQLDHNGINFALNIRGQVHPVHSPLLGRFNVDNLLAVAGALWAMEFAPA
QIASVLGQLQPIHGRMNRLGGAHGAPLVVVDYAHTPDALEQALSSLRSHAQDRLICVFGCGGERDTGKRPQMAAIAELKA
DVAIVTDDNPRGEDGDAIVADILRGFARPDAAIVQRDRAAAIHQAIGMASPSDIVLIAGKGHEPYQEVAGVRHAFDDALV
AAQALLPRTVLGVRP

Sequences:

>Translated_495_residues
MSRSMALSQLLPDVALTHDVQVSGLVMDSRAVRPGDAFVAIAGFGAHGLGFVEQARANGAAAVLFEPPAPDGLPVPADAI
AVPGLRARLGVMADQFHGRPSQAMRMVGVTGTNGKTSTVQLLAQALTLLGTPTGTLGTLGVGLYGAAVPTGFTTPLVLPT
HALLAQLRDDGAQAVAMEVSSHALDQGRVDAVHFDVAVFTNLTRDHLDYHGDMAQYGAAKAKLFARAGLKAAVVNLDDAF
GHTLFAALDPALRAIGVSSRAHADASVSAQALQLDHNGINFALNIRGQVHPVHSPLLGRFNVDNLLAVAGALWAMEFAPA
QIASVLGQLQPIHGRMNRLGGAHGAPLVVVDYAHTPDALEQALSSLRSHAQDRLICVFGCGGERDTGKRPQMAAIAELKA
DVAIVTDDNPRGEDGDAIVADILRGFARPDAAIVQRDRAAAIHQAIGMASPSDIVLIAGKGHEPYQEVAGVRHAFDDALV
AAQALLPRTVLGVRP
>Mature_494_residues
SRSMALSQLLPDVALTHDVQVSGLVMDSRAVRPGDAFVAIAGFGAHGLGFVEQARANGAAAVLFEPPAPDGLPVPADAIA
VPGLRARLGVMADQFHGRPSQAMRMVGVTGTNGKTSTVQLLAQALTLLGTPTGTLGTLGVGLYGAAVPTGFTTPLVLPTH
ALLAQLRDDGAQAVAMEVSSHALDQGRVDAVHFDVAVFTNLTRDHLDYHGDMAQYGAAKAKLFARAGLKAAVVNLDDAFG
HTLFAALDPALRAIGVSSRAHADASVSAQALQLDHNGINFALNIRGQVHPVHSPLLGRFNVDNLLAVAGALWAMEFAPAQ
IASVLGQLQPIHGRMNRLGGAHGAPLVVVDYAHTPDALEQALSSLRSHAQDRLICVFGCGGERDTGKRPQMAAIAELKAD
VAIVTDDNPRGEDGDAIVADILRGFARPDAAIVQRDRAAAIHQAIGMASPSDIVLIAGKGHEPYQEVAGVRHAFDDALVA
AQALLPRTVLGVRP

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=475, Percent_Identity=44.421052631579, Blast_Score=308, Evalue=5e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_XANAC (Q8PPB2)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641127.1
- ProteinModelPortal:   Q8PPB2
- SMR:   Q8PPB2
- GeneID:   1154846
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0775
- NMPDR:   fig|190486.1.peg.771
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- ProtClustDB:   PRK00139
- BioCyc:   XAXO190486:XAC0775-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 51425; Mature: 51294

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: BINDING 29-29 BINDING 180-180 BINDING 186-186 BINDING 188-188 BINDING 384-384 BINDING 459-459 BINDING 463-463

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSMALSQLLPDVALTHDVQVSGLVMDSRAVRPGDAFVAIAGFGAHGLGFVEQARANGA
CCCHHHHHHHCCHHHHCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCC
AAVLFEPPAPDGLPVPADAIAVPGLRARLGVMADQFHGRPSQAMRMVGVTGTNGKTSTVQ
EEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCHHHHEEEEEEECCCCCHHHHH
LLAQALTLLGTPTGTLGTLGVGLYGAAVPTGFTTPLVLPTHALLAQLRDDGAQAVAMEVS
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHH
SHALDQGRVDAVHFDVAVFTNLTRDHLDYHGDMAQYGAAKAKLFARAGLKAAVVNLDDAF
HHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHCCCEEEEEECCHHH
GHTLFAALDPALRAIGVSSRAHADASVSAQALQLDHNGINFALNIRGQVHPVHSPLLGRF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCEEEECCCCCCCCC
NVDNLLAVAGALWAMEFAPAQIASVLGQLQPIHGRMNRLGGAHGAPLVVVDYAHTPDALE
CHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHH
QALSSLRSHAQDRLICVFGCGGERDTGKRPQMAAIAELKADVAIVTDDNPRGEDGDAIVA
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCHHHH
DILRGFARPDAAIVQRDRAAAIHQAIGMASPSDIVLIAGKGHEPYQEVAGVRHAFDDALV
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
AAQALLPRTVLGVRP
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRSMALSQLLPDVALTHDVQVSGLVMDSRAVRPGDAFVAIAGFGAHGLGFVEQARANGA
CCHHHHHHHCCHHHHCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCC
AAVLFEPPAPDGLPVPADAIAVPGLRARLGVMADQFHGRPSQAMRMVGVTGTNGKTSTVQ
EEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCHHHHEEEEEEECCCCCHHHHH
LLAQALTLLGTPTGTLGTLGVGLYGAAVPTGFTTPLVLPTHALLAQLRDDGAQAVAMEVS
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHH
SHALDQGRVDAVHFDVAVFTNLTRDHLDYHGDMAQYGAAKAKLFARAGLKAAVVNLDDAF
HHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHCCCEEEEEECCHHH
GHTLFAALDPALRAIGVSSRAHADASVSAQALQLDHNGINFALNIRGQVHPVHSPLLGRF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCEEEECCCCCCCCC
NVDNLLAVAGALWAMEFAPAQIASVLGQLQPIHGRMNRLGGAHGAPLVVVDYAHTPDALE
CHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHH
QALSSLRSHAQDRLICVFGCGGERDTGKRPQMAAIAELKADVAIVTDDNPRGEDGDAIVA
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCHHHH
DILRGFARPDAAIVQRDRAAAIHQAIGMASPSDIVLIAGKGHEPYQEVAGVRHAFDDALV
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
AAQALLPRTVLGVRP
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217