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The map label for this gene is murF [H]

Identifier: 21241546

GI number: 21241546

Start: 919911

End: 921422

Strand: Direct

Name: murF [H]

Synonym: XAC0776

Alternate gene names: 21241546

Gene position: 919911-921422 (Clockwise)

Preceding gene: 21241545

Following gene: 21241547

Centisome position: 17.77

GC content: 68.58

Gene sequence:

>1512_bases
ATGAAGCTCACGCCCCTGTCGTTGATTGCCCATTGGGCCGGCGGCGAGCTGCACGGCGACGACGCCATGATCGATGCGGT
CGGCAACGACACCCGCACGCTCGCCGACGGCAGCCTGTATGTGGCGCTGCGCGGCGAGCGCTTCGATGGGCACGACTTCG
CCGCCGAGGCGGTGGCGCGTGGTGCCAGCGGTGTGCTGGTCGAACGCCTGCTGCCGGCGCTGAGCGTGCCGCAGGTGGTG
GTGGACAACACCGAGCTGGCGTTGGCGCGCATCGCCGCCGGCATGCAGCGCGACCGCGCCACGCGTGTGCTTGCGCTGAC
CGGCTCCAACGGCAAGACCAGCGTCAAGGCGCTGCTGCTGTCGATCCTGACCGAAGCCGGGCGCGTGGATGGCACCACCG
TGTACGCCACGCCGGGCAACCGCAACAACGAGATCGGCCTGCCGCTGGCGGTGCTTGCGGCGCCCGACGATGCGGACGTT
GCGATCTACGAAATGGGCGCGGGCAAGCCGGGCGATATCGCCTATCTCACCGAGATTGCGCAGCCGCATGTGGCACTGGT
CAACAACATCGCGCCCGCGCATCTGGAACGCCTGGGCAGCTTGCTCGGCGTCGCACAGACCAAGGGCGCCATCTACGCCG
CATTGCCCGCCGGGGGTGTGGCGGTGATCAACGCCGACGACGCATTCGGCATGTGGTTCGAGCAGCAGTTGCAGAGCCAA
CCGGTGCAGGGACGCCGCGTGTTGCGCTTTGGCGTGCAGGCCAGTGCCGAGATCACTGCGCGGCAACTGCGTCTGCACCC
TGGCGGTGCGCAGTTCACCCTGGTGACGCCGCAGGGCGAGGCACAGATTGCGCTGCCGTTGCCGGGCCGCCACAACGTGC
TCAACGCACTGGCGGCAACCGGCCTTGCACTCGGTGCCGGCATCGCCTTGCCGGTGATCGCGGCCGGTCTGGCGCAGGCG
CGCGCGGTGGCCGGCCGGCAGATCGCACAGACCTTGCCCAATGGCGCGGTACTGATCGACGACAGCTACAACGCCAACCC
GGGCTCGCTGGATGCGGCCATCGACGCCTTGGCCGCGGCACAGGGCGAAGGCTGGCTGGTGCTGGGCGACATGCGCGAAC
TCGGTGCCGAAGGCGTCGCATTGCATGCCGCGGCGGGACGTCGCGCGCGTGCGGCCAAGCTGCAGCGGCTGTATGCGCTG
GGCGAACTCAGCGCCGCCGCGGCGCAGGCATTCGGCGAAGGTGGCCGCGTGTTCGCCACGCATGCCGAGTTGATCGACGC
CTTGCAGGCGGATCTGGTCGGAGCCGCCAGCCAGCAACAGCAAATGCAGCAACACGCATCAGGAAATACGCAGGAACATG
CAGTGAATCAAGAACAGCAATCGCCGTCTGTGGCCGCTCTTTCGGGTGCCGCCTCCATCACGATCCTGGTGAAGGGCTCG
CGCGGTAGCGCGATGGACCGCGTCGTCACCGCGCTGCTCGCATCGGCGGAGGGCGCATCCCATGCTGCTTGA

Upstream 100 bases:

>100_bases
ACGAGCCGTATCAGGAAGTTGCCGGCGTGCGGCATGCCTTCGACGACGCCCTCGTGGCGGCGCAGGCGCTGCTGCCACGG
ACCGTATTGGGAGTGCGCCC

Downstream 100 bases:

>100_bases
GTTGGCCCGTTGGCTGCAGCAACTGGAGAGCCTGTTCGGGCTGTTCAACTATCTGACCTTCCGCGGCATCTTGGCTGCGC
TGACGGCGCTGTTCCTGTCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MKLTPLSLIAHWAGGELHGDDAMIDAVGNDTRTLADGSLYVALRGERFDGHDFAAEAVARGASGVLVERLLPALSVPQVV
VDNTELALARIAAGMQRDRATRVLALTGSNGKTSVKALLLSILTEAGRVDGTTVYATPGNRNNEIGLPLAVLAAPDDADV
AIYEMGAGKPGDIAYLTEIAQPHVALVNNIAPAHLERLGSLLGVAQTKGAIYAALPAGGVAVINADDAFGMWFEQQLQSQ
PVQGRRVLRFGVQASAEITARQLRLHPGGAQFTLVTPQGEAQIALPLPGRHNVLNALAATGLALGAGIALPVIAAGLAQA
RAVAGRQIAQTLPNGAVLIDDSYNANPGSLDAAIDALAAAQGEGWLVLGDMRELGAEGVALHAAAGRRARAAKLQRLYAL
GELSAAAAQAFGEGGRVFATHAELIDALQADLVGAASQQQQMQQHASGNTQEHAVNQEQQSPSVAALSGAASITILVKGS
RGSAMDRVVTALLASAEGASHAA

Sequences:

>Translated_503_residues
MKLTPLSLIAHWAGGELHGDDAMIDAVGNDTRTLADGSLYVALRGERFDGHDFAAEAVARGASGVLVERLLPALSVPQVV
VDNTELALARIAAGMQRDRATRVLALTGSNGKTSVKALLLSILTEAGRVDGTTVYATPGNRNNEIGLPLAVLAAPDDADV
AIYEMGAGKPGDIAYLTEIAQPHVALVNNIAPAHLERLGSLLGVAQTKGAIYAALPAGGVAVINADDAFGMWFEQQLQSQ
PVQGRRVLRFGVQASAEITARQLRLHPGGAQFTLVTPQGEAQIALPLPGRHNVLNALAATGLALGAGIALPVIAAGLAQA
RAVAGRQIAQTLPNGAVLIDDSYNANPGSLDAAIDALAAAQGEGWLVLGDMRELGAEGVALHAAAGRRARAAKLQRLYAL
GELSAAAAQAFGEGGRVFATHAELIDALQADLVGAASQQQQMQQHASGNTQEHAVNQEQQSPSVAALSGAASITILVKGS
RGSAMDRVVTALLASAEGASHAA
>Mature_503_residues
MKLTPLSLIAHWAGGELHGDDAMIDAVGNDTRTLADGSLYVALRGERFDGHDFAAEAVARGASGVLVERLLPALSVPQVV
VDNTELALARIAAGMQRDRATRVLALTGSNGKTSVKALLLSILTEAGRVDGTTVYATPGNRNNEIGLPLAVLAAPDDADV
AIYEMGAGKPGDIAYLTEIAQPHVALVNNIAPAHLERLGSLLGVAQTKGAIYAALPAGGVAVINADDAFGMWFEQQLQSQ
PVQGRRVLRFGVQASAEITARQLRLHPGGAQFTLVTPQGEAQIALPLPGRHNVLNALAATGLALGAGIALPVIAAGLAQA
RAVAGRQIAQTLPNGAVLIDDSYNANPGSLDAAIDALAAAQGEGWLVLGDMRELGAEGVALHAAAGRRARAAKLQRLYAL
GELSAAAAQAFGEGGRVFATHAELIDALQADLVGAASQQQQMQQHASGNTQEHAVNQEQQSPSVAALSGAASITILVKGS
RGSAMDRVVTALLASAEGASHAA

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=488, Percent_Identity=42.827868852459, Blast_Score=306, Evalue=3e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51471; Mature: 51471

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTPLSLIAHWAGGELHGDDAMIDAVGNDTRTLADGSLYVALRGERFDGHDFAAEAVAR
CCCCHHHHHHHHCCCEECCCCCEEEECCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHC
GASGVLVERLLPALSVPQVVVDNTELALARIAAGMQRDRATRVLALTGSNGKTSVKALLL
CCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
SILTEAGRVDGTTVYATPGNRNNEIGLPLAVLAAPDDADVAIYEMGAGKPGDIAYLTEIA
HHHHHCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHHHC
QPHVALVNNIAPAHLERLGSLLGVAQTKGAIYAALPAGGVAVINADDAFGMWFEQQLQSQ
CCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHCC
PVQGRRVLRFGVQASAEITARQLRLHPGGAQFTLVTPQGEAQIALPLPGRHNVLNALAAT
CCCCCHHHHCCCCCCHHEEEEEEEECCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHH
GLALGAGIALPVIAAGLAQARAVAGRQIAQTLPNGAVLIDDSYNANPGSLDAAIDALAAA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHC
QGEGWLVLGDMRELGAEGVALHAAAGRRARAAKLQRLYALGELSAAAAQAFGEGGRVFAT
CCCCEEEECCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
HAELIDALQADLVGAASQQQQMQQHASGNTQEHAVNQEQQSPSVAALSGAASITILVKGS
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHCCHHHCCCCEEEECCCEEEEEEEECC
RGSAMDRVVTALLASAEGASHAA
CCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLTPLSLIAHWAGGELHGDDAMIDAVGNDTRTLADGSLYVALRGERFDGHDFAAEAVAR
CCCCHHHHHHHHCCCEECCCCCEEEECCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHC
GASGVLVERLLPALSVPQVVVDNTELALARIAAGMQRDRATRVLALTGSNGKTSVKALLL
CCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
SILTEAGRVDGTTVYATPGNRNNEIGLPLAVLAAPDDADVAIYEMGAGKPGDIAYLTEIA
HHHHHCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHHHC
QPHVALVNNIAPAHLERLGSLLGVAQTKGAIYAALPAGGVAVINADDAFGMWFEQQLQSQ
CCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHCC
PVQGRRVLRFGVQASAEITARQLRLHPGGAQFTLVTPQGEAQIALPLPGRHNVLNALAAT
CCCCCHHHHCCCCCCHHEEEEEEEECCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHH
GLALGAGIALPVIAAGLAQARAVAGRQIAQTLPNGAVLIDDSYNANPGSLDAAIDALAAA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHC
QGEGWLVLGDMRELGAEGVALHAAAGRRARAAKLQRLYALGELSAAAAQAFGEGGRVFAT
CCCCEEEECCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
HAELIDALQADLVGAASQQQQMQQHASGNTQEHAVNQEQQSPSVAALSGAASITILVKGS
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHCCHHHCCCCEEEECCCEEEEEEEECC
RGSAMDRVVTALLASAEGASHAA
CCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]