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The map label for this gene is peh-1 [H]

Identifier: 21241432

GI number: 21241432

Start: 783450

End: 784841

Strand: Direct

Name: peh-1 [H]

Synonym: XAC0661

Alternate gene names: 21241432

Gene position: 783450-784841 (Clockwise)

Preceding gene: 21241431

Following gene: 21241433

Centisome position: 15.14

GC content: 64.37

Gene sequence:

>1392_bases
ATGAAACTGCTTACCGCTGCCATCGGCGGCGTTTGTCTGACGCTTGCTGCGCAAGCCGGCGCCGTTACGATCGCTACCGG
CGATACGCGCGCTGTCGTGCAACCGGCGATTCCCGCCACTTGTCAGCCGTCCTTGAAGGCCAGCCAGACGCCGACCGGGC
GGCAATTCGACGCCGCGCTGGAAAGCGCGCCCCCCGATACCAAGGCCATCCAGGCCGCGCTCAACGCCTGCAAGAACGGA
AGCGTGCTGTTGACCAGCGGCAGTGGCAACGCGTTTCTGACCGGGCCGTTGTCCATCCCGGCCAACGTCACCCTGGTCAT
CGACCAGGGCGTAACCTTGTACGGCTCGCGCAACCCGGCCGACTACGGCAGCGGTTGCGGCACCGCCGGTTCCAAGAGCG
GTGCTTGCCTGCCGTTGATCAGCGTCAAGGGCAACAACGCAGGCGTGATGGGCATCCGCACCGGCGGGCGTCAGGGCACC
ATCGATGGGCGCGGCGACCAGACCATGCTGGGCAAGAGCACCACCTGGTGGGAGTTCGGCGAAAACGCCAAGAAGGCCAA
TCAGGTGCAGAACAGCCCGGACCTAATCAAGGTGCAGAATTCCAGCGAGTTCACGCTCTACGACGTCAACCTGATCAATG
CGGCGTACTTCCATTTCTTCTCGCATATCGTCAATGGCCTCACCATCTGGGGCGTGCGGGTGAGATCGCCGGCCACCAGC
CCCAATACCGACGGCCTGGATCTGGACAGCGTGGTCAACGCCACCATCCACGACAGCGATGTGATGGGCGGCGACGACTG
CGTGGCGATCAAGACCATCGCCTCCAGGTCGGCGAACATCACCGTGCGCAACTCGCGCTGCTACGGCACCCACGGCATCT
CGATCGGAAGCGAAGTGATGTCCGGCGTCAGCAATGTGCTGGTCGACAATAATGCGCTGGTGTCCACCGACGATCTGGGC
AACCGCAGCACCGACAACAGCGGCCTGCGCATCAAGACCAGCCTCGCCAAGGGTGGTCCGGTCAGTCTGATTACCTATCG
CAACACCTGCCTGTACGGCGTCAGCAGCCCGGTGGTGATCAACCCGTTCTACGCCGCCGCAACCAGCGGTACCAAACCGA
CCTTCAGCCAGATCGTGGTCGATGGGCTCAAAACCGGCAACGACGCCGGCGGCAAGGGCTGGATCCTGAAAGGCTTCGAT
GCGCAGACACCGCTGGATCTGGTGTTGGCCAACGTGGCGCACAGCGACGCGTCGATCAGCGCCAGCAACGCACAGATCGG
GCTGAGCAACAGCGCCGTCACGCCGTCGGGGACTGGCGTCACCACCGGCACCGTGCAGGTCGGCGGTGTGGTGCCCACCT
GCTCGGGCGCACCGGGCTTCCCCGCGCTGTGA

Upstream 100 bases:

>100_bases
CCACGCTCCGTAGAGTCCGCCACGCCGCTGCAGCGATGGCGGTGCGCACCGCGTGGCCTGCACGGGCACGCGCACTTTAC
TCTTTCGGGTGATCTCTTCT

Downstream 100 bases:

>100_bases
TCTCGCTGGCGCGGCCGTCACTCGCCGCGCCAGACCCGCTGGTGCCTCGCCCGCGAACGTACGTCTTTCAGCATGGCAGA
TAGGGCTGCGGCCCCATACA

Product: endopolygalacturonase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKLLTAAIGGVCLTLAAQAGAVTIATGDTRAVVQPAIPATCQPSLKASQTPTGRQFDAALESAPPDTKAIQAALNACKNG
SVLLTSGSGNAFLTGPLSIPANVTLVIDQGVTLYGSRNPADYGSGCGTAGSKSGACLPLISVKGNNAGVMGIRTGGRQGT
IDGRGDQTMLGKSTTWWEFGENAKKANQVQNSPDLIKVQNSSEFTLYDVNLINAAYFHFFSHIVNGLTIWGVRVRSPATS
PNTDGLDLDSVVNATIHDSDVMGGDDCVAIKTIASRSANITVRNSRCYGTHGISIGSEVMSGVSNVLVDNNALVSTDDLG
NRSTDNSGLRIKTSLAKGGPVSLITYRNTCLYGVSSPVVINPFYAAATSGTKPTFSQIVVDGLKTGNDAGGKGWILKGFD
AQTPLDLVLANVAHSDASISASNAQIGLSNSAVTPSGTGVTTGTVQVGGVVPTCSGAPGFPAL

Sequences:

>Translated_463_residues
MKLLTAAIGGVCLTLAAQAGAVTIATGDTRAVVQPAIPATCQPSLKASQTPTGRQFDAALESAPPDTKAIQAALNACKNG
SVLLTSGSGNAFLTGPLSIPANVTLVIDQGVTLYGSRNPADYGSGCGTAGSKSGACLPLISVKGNNAGVMGIRTGGRQGT
IDGRGDQTMLGKSTTWWEFGENAKKANQVQNSPDLIKVQNSSEFTLYDVNLINAAYFHFFSHIVNGLTIWGVRVRSPATS
PNTDGLDLDSVVNATIHDSDVMGGDDCVAIKTIASRSANITVRNSRCYGTHGISIGSEVMSGVSNVLVDNNALVSTDDLG
NRSTDNSGLRIKTSLAKGGPVSLITYRNTCLYGVSSPVVINPFYAAATSGTKPTFSQIVVDGLKTGNDAGGKGWILKGFD
AQTPLDLVLANVAHSDASISASNAQIGLSNSAVTPSGTGVTTGTVQVGGVVPTCSGAPGFPAL
>Mature_463_residues
MKLLTAAIGGVCLTLAAQAGAVTIATGDTRAVVQPAIPATCQPSLKASQTPTGRQFDAALESAPPDTKAIQAALNACKNG
SVLLTSGSGNAFLTGPLSIPANVTLVIDQGVTLYGSRNPADYGSGCGTAGSKSGACLPLISVKGNNAGVMGIRTGGRQGT
IDGRGDQTMLGKSTTWWEFGENAKKANQVQNSPDLIKVQNSSEFTLYDVNLINAAYFHFFSHIVNGLTIWGVRVRSPATS
PNTDGLDLDSVVNATIHDSDVMGGDDCVAIKTIASRSANITVRNSRCYGTHGISIGSEVMSGVSNVLVDNNALVSTDDLG
NRSTDNSGLRIKTSLAKGGPVSLITYRNTCLYGVSSPVVINPFYAAATSGTKPTFSQIVVDGLKTGNDAGGKGWILKGFD
AQTPLDLVLANVAHSDASISASNAQIGLSNSAVTPSGTGVTTGTVQVGGVVPTCSGAPGFPAL

Specific function: Involved in maceration and soft-rotting of plant tissue [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 28 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322613, Length=244, Percent_Identity=27.8688524590164, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 47138; Mature: 47138

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLTAAIGGVCLTLAAQAGAVTIATGDTRAVVQPAIPATCQPSLKASQTPTGRQFDAAL
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
ESAPPDTKAIQAALNACKNGSVLLTSGSGNAFLTGPLSIPANVTLVIDQGVTLYGSRNPA
HCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCEEEEEEECCEEEEECCCCC
DYGSGCGTAGSKSGACLPLISVKGNNAGVMGIRTGGRQGTIDGRGDQTMLGKSTTWWEFG
CCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCHHCC
ENAKKANQVQNSPDLIKVQNSSEFTLYDVNLINAAYFHFFSHIVNGLTIWGVRVRSPATS
CCHHHHHHCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCEEEEEEEEECCCCC
PNTDGLDLDSVVNATIHDSDVMGGDDCVAIKTIASRSANITVRNSRCYGTHGISIGSEVM
CCCCCCCHHHHHCCEEECCCCCCCCCEEEEEEECCCCCEEEEECCEEECCCCCCCHHHHH
SGVSNVLVDNNALVSTDDLGNRSTDNSGLRIKTSLAKGGPVSLITYRNTCLYGVSSPVVI
HHHHHHEECCCCEEECCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECEEEECCCCCEEE
NPFYAAATSGTKPTFSQIVVDGLKTGNDAGGKGWILKGFDAQTPLDLVLANVAHSDASIS
CCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCEE
ASNAQIGLSNSAVTPSGTGVTTGTVQVGGVVPTCSGAPGFPAL
CCCCEEECCCCCCCCCCCCEEEEEEEECCEEEECCCCCCCCCC
>Mature Secondary Structure
MKLLTAAIGGVCLTLAAQAGAVTIATGDTRAVVQPAIPATCQPSLKASQTPTGRQFDAAL
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
ESAPPDTKAIQAALNACKNGSVLLTSGSGNAFLTGPLSIPANVTLVIDQGVTLYGSRNPA
HCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCEEEEEEECCEEEEECCCCC
DYGSGCGTAGSKSGACLPLISVKGNNAGVMGIRTGGRQGTIDGRGDQTMLGKSTTWWEFG
CCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCHHCC
ENAKKANQVQNSPDLIKVQNSSEFTLYDVNLINAAYFHFFSHIVNGLTIWGVRVRSPATS
CCHHHHHHCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCEEEEEEEEECCCCC
PNTDGLDLDSVVNATIHDSDVMGGDDCVAIKTIASRSANITVRNSRCYGTHGISIGSEVM
CCCCCCCHHHHHCCEEECCCCCCCCCEEEEEEECCCCCEEEEECCEEECCCCCCCHHHHH
SGVSNVLVDNNALVSTDDLGNRSTDNSGLRIKTSLAKGGPVSLITYRNTCLYGVSSPVVI
HHHHHHEECCCCEEECCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECEEEECCCCCEEE
NPFYAAATSGTKPTFSQIVVDGLKTGNDAGGKGWILKGFDAQTPLDLVLANVAHSDASIS
CCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCEE
ASNAQIGLSNSAVTPSGTGVTTGTVQVGGVVPTCSGAPGFPAL
CCCCEEECCCCCCCCCCCCEEEEEEEECCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2215211; 8074530; 8407675 [H]