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The map label for this gene is mls [H]

Identifier: 21241030

GI number: 21241030

Start: 304727

End: 306343

Strand: Direct

Name: mls [H]

Synonym: XAC0256

Alternate gene names: 21241030

Gene position: 304727-306343 (Clockwise)

Preceding gene: 21241025

Following gene: 21241031

Centisome position: 5.89

GC content: 66.36

Gene sequence:

>1617_bases
ATGGCTGCCACCGCGTTTGCTCCCCGCCCCAACGACCACGCCACGCCCGGCATTGCGCTGACCACCCAGATCGCCGGCCA
GGCGGAGCTGCTGCCGGCCGCTGCGCTTGCCTTGCTGGTCTCGTTGCACCGGGCGATCGAGCCCGGCCGCCAACAGCGTC
TGGCCCAGCGGCGCCAGCGCCAGGCCGCCTTCGACGCCGGTCAGCTGCCAGACTTCCGTACCGACACCCAGGCCATCCGT
ACCGGCGACTGGCGCGTGGCCCCTCTGCCGGTGGCGCTGCAGGACCGTCGCGTCGAAATCACCGGCCCAACCGACCCGAA
GATGGTCATCAACGCGCTGAACTCCGGCGCCAAGGTGTTCATGGCCGACTTCGAAGATTCCACCGCGCCGACCTGGCGCA
ACCTGCTGGCCGGCCAGCGCACCCTGGTTGCGGCGGTGCGCGGCGATCTCAGCTTCGATGCGCCCAACGGCAAGCATTAC
GCCCTGCGCCCGGAGGCCGAACGTGCGGTGCTGATCGTGCGTCCGCGTGGTTGGCACCTGGACGAAAAGCACGTGCTGAT
CGACGGCCAGCCGCTGGCCGGCGGCCTGTTCGATGCGGCGTTATTTGCCTTCCACAATGGCCGCACGTTGCTGGCCAAGG
ACCGCGGCCCGTACCTGTATCTGCCCAAGCTGCAGAGCATGGAAGAAGCCGCACTGTGGGACACCGCGCTGGCGCATATC
GAAGCGATGCTGGGCCTGCCGCATGGGCAGATCAAGGTCACCGTGCTGATCGAAACGCTGCCGGCGGTGTTTGAGATGGA
CGAAATTCTCTACGCGCTGCGCGAGCGCATCGTTGGGCTCAATTGCGGGCGTTGGGACTACATTTTTTCCTACCTGAAAA
CCTTTCGTGCGCACCGCGACCGCGTGCTGCCCGAACGCGGCCAGGTCACCATGACCCAGCCATTCTTGAGGGCGTATTCG
GAGCTGTTGATCAAGACCTGCCACCGCCGCGGCGCGCATGCGATGGGCGGCATGGCCGCGCAGATTCCGATCAACCACGA
TGAAGCCGCCAACGAACAGGCCATGGCCCGCGTGCGCGCCGACAAGCTGCGCGAAGTGAGCGCCGGCCACGATGGCACCT
GGGTGGCGCATCCGGCGCTGATTCCGGTGGCGATGAAACTGTTCGACGAACACATGCCCACCGCGCATCAGCAGCATGTG
TTGCGCAACGACGTGCAGGTGACGCGCGACCTGCTGATCGCGCCCTCGCCGGGCAATGTCACCCGCGCCGGCTTCGAAGG
CAATGTCGAAGTATGCGTGCGCTATCTGGCGGCCTGGCTGGACGGCAATGGCTGCGTGCCGATCCACCACCTGATGGAAG
ACGCCGCCACGGCCGAAATCAGCCGCGCGCAGCTGTGGCAATGGTTGCACCACGGTCAGCACCTGGATGATGGCACAGCC
ATCGATCAACACCTGCTGCAGGCGACCCTGCGCGCACTGCCCGCACGGTTGGGAACCGCCACCGCGCTGCCCGGCGCTGC
ACGGATCGATGAAGCGATCGCCTTGCTCGAAGAGTTGAGCGGCGCCGACGAACTGGCGGACTTCCTTACTGTACCGGCGT
ACCGCCTGATCGACTGA

Upstream 100 bases:

>100_bases
TTTAATATAAGCCAGACTTATTTACAGCATTGATAAAACTGCTTTGTCAAATAGTTGTATCGGGCGCACGGTGCAGGTCC
GGATGCACAAGGACCTCCAC

Downstream 100 bases:

>100_bases
TCGCCAATGCGGCGACGCCTCGCCACCGGCGTCGCGCAGCTTCCACTTCAACGCAGTCAACGCCCGTTGCGCACGCACGC
GCAACGACGGCCTCGCCATG

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MAATAFAPRPNDHATPGIALTTQIAGQAELLPAAALALLVSLHRAIEPGRQQRLAQRRQRQAAFDAGQLPDFRTDTQAIR
TGDWRVAPLPVALQDRRVEITGPTDPKMVINALNSGAKVFMADFEDSTAPTWRNLLAGQRTLVAAVRGDLSFDAPNGKHY
ALRPEAERAVLIVRPRGWHLDEKHVLIDGQPLAGGLFDAALFAFHNGRTLLAKDRGPYLYLPKLQSMEEAALWDTALAHI
EAMLGLPHGQIKVTVLIETLPAVFEMDEILYALRERIVGLNCGRWDYIFSYLKTFRAHRDRVLPERGQVTMTQPFLRAYS
ELLIKTCHRRGAHAMGGMAAQIPINHDEAANEQAMARVRADKLREVSAGHDGTWVAHPALIPVAMKLFDEHMPTAHQQHV
LRNDVQVTRDLLIAPSPGNVTRAGFEGNVEVCVRYLAAWLDGNGCVPIHHLMEDAATAEISRAQLWQWLHHGQHLDDGTA
IDQHLLQATLRALPARLGTATALPGAARIDEAIALLEELSGADELADFLTVPAYRLID

Sequences:

>Translated_538_residues
MAATAFAPRPNDHATPGIALTTQIAGQAELLPAAALALLVSLHRAIEPGRQQRLAQRRQRQAAFDAGQLPDFRTDTQAIR
TGDWRVAPLPVALQDRRVEITGPTDPKMVINALNSGAKVFMADFEDSTAPTWRNLLAGQRTLVAAVRGDLSFDAPNGKHY
ALRPEAERAVLIVRPRGWHLDEKHVLIDGQPLAGGLFDAALFAFHNGRTLLAKDRGPYLYLPKLQSMEEAALWDTALAHI
EAMLGLPHGQIKVTVLIETLPAVFEMDEILYALRERIVGLNCGRWDYIFSYLKTFRAHRDRVLPERGQVTMTQPFLRAYS
ELLIKTCHRRGAHAMGGMAAQIPINHDEAANEQAMARVRADKLREVSAGHDGTWVAHPALIPVAMKLFDEHMPTAHQQHV
LRNDVQVTRDLLIAPSPGNVTRAGFEGNVEVCVRYLAAWLDGNGCVPIHHLMEDAATAEISRAQLWQWLHHGQHLDDGTA
IDQHLLQATLRALPARLGTATALPGAARIDEAIALLEELSGADELADFLTVPAYRLID
>Mature_537_residues
AATAFAPRPNDHATPGIALTTQIAGQAELLPAAALALLVSLHRAIEPGRQQRLAQRRQRQAAFDAGQLPDFRTDTQAIRT
GDWRVAPLPVALQDRRVEITGPTDPKMVINALNSGAKVFMADFEDSTAPTWRNLLAGQRTLVAAVRGDLSFDAPNGKHYA
LRPEAERAVLIVRPRGWHLDEKHVLIDGQPLAGGLFDAALFAFHNGRTLLAKDRGPYLYLPKLQSMEEAALWDTALAHIE
AMLGLPHGQIKVTVLIETLPAVFEMDEILYALRERIVGLNCGRWDYIFSYLKTFRAHRDRVLPERGQVTMTQPFLRAYSE
LLIKTCHRRGAHAMGGMAAQIPINHDEAANEQAMARVRADKLREVSAGHDGTWVAHPALIPVAMKLFDEHMPTAHQQHVL
RNDVQVTRDLLIAPSPGNVTRAGFEGNVEVCVRYLAAWLDGNGCVPIHHLMEDAATAEISRAQLWQWLHHGQHLDDGTAI
DQHLLQATLRALPARLGTATALPGAARIDEAIALLEELSGADELADFLTVPAYRLID

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=517, Percent_Identity=52.0309477756286, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI1789348, Length=475, Percent_Identity=24, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17561814, Length=526, Percent_Identity=53.8022813688213, Blast_Score=543, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI71982926, Length=420, Percent_Identity=54.047619047619, Blast_Score=446, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6324212, Length=513, Percent_Identity=45.4191033138402, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322222, Length=514, Percent_Identity=46.1089494163424, Blast_Score=453, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 59273; Mature: 59141

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATAFAPRPNDHATPGIALTTQIAGQAELLPAAALALLVSLHRAIEPGRQQRLAQRRQR
CCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
QAAFDAGQLPDFRTDTQAIRTGDWRVAPLPVALQDRRVEITGPTDPKMVINALNSGAKVF
HHHCCCCCCCCCCCCHHHHCCCCEEEECCCEEECCCEEEEECCCCHHHHHHHHCCCCEEE
MADFEDSTAPTWRNLLAGQRTLVAAVRGDLSFDAPNGKHYALRPEAERAVLIVRPRGWHL
EEECCCCCCHHHHHHHCCCHHHHHHHHCCCEECCCCCCEEEECCCCCCEEEEECCCCCCC
DEKHVLIDGQPLAGGLFDAALFAFHNGRTLLAKDRGPYLYLPKLQSMEEAALWDTALAHI
CCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCHHHHHHHHHHHHHH
EAMLGLPHGQIKVTVLIETLPAVFEMDEILYALRERIVGLNCGRWDYIFSYLKTFRAHRD
HHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RVLPERGQVTMTQPFLRAYSELLIKTCHRRGAHAMGGMAAQIPINHDEAANEQAMARVRA
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCHHCCHHHHHHHHH
DKLREVSAGHDGTWVAHPALIPVAMKLFDEHMPTAHQQHVLRNDVQVTRDLLIAPSPGNV
HHHHHHCCCCCCCEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCC
TRAGFEGNVEVCVRYLAAWLDGNGCVPIHHLMEDAATAEISRAQLWQWLHHGQHLDDGTA
EECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
IDQHLLQATLRALPARLGTATALPGAARIDEAIALLEELSGADELADFLTVPAYRLID
HHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
AATAFAPRPNDHATPGIALTTQIAGQAELLPAAALALLVSLHRAIEPGRQQRLAQRRQR
CCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
QAAFDAGQLPDFRTDTQAIRTGDWRVAPLPVALQDRRVEITGPTDPKMVINALNSGAKVF
HHHCCCCCCCCCCCCHHHHCCCCEEEECCCEEECCCEEEEECCCCHHHHHHHHCCCCEEE
MADFEDSTAPTWRNLLAGQRTLVAAVRGDLSFDAPNGKHYALRPEAERAVLIVRPRGWHL
EEECCCCCCHHHHHHHCCCHHHHHHHHCCCEECCCCCCEEEECCCCCCEEEEECCCCCCC
DEKHVLIDGQPLAGGLFDAALFAFHNGRTLLAKDRGPYLYLPKLQSMEEAALWDTALAHI
CCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCHHHHHHHHHHHHHH
EAMLGLPHGQIKVTVLIETLPAVFEMDEILYALRERIVGLNCGRWDYIFSYLKTFRAHRD
HHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RVLPERGQVTMTQPFLRAYSELLIKTCHRRGAHAMGGMAAQIPINHDEAANEQAMARVRA
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCHHCCHHHHHHHHH
DKLREVSAGHDGTWVAHPALIPVAMKLFDEHMPTAHQQHVLRNDVQVTRDLLIAPSPGNV
HHHHHHCCCCCCCEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCC
TRAGFEGNVEVCVRYLAAWLDGNGCVPIHHLMEDAATAEISRAQLWQWLHHGQHLDDGTA
EECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
IDQHLLQATLRALPARLGTATALPGAARIDEAIALLEELSGADELADFLTVPAYRLID
HHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA