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The map label for this gene is adh [H]

Identifier: 21240975

GI number: 21240975

Start: 247700

End: 248710

Strand: Direct

Name: adh [H]

Synonym: XAC0201

Alternate gene names: 21240975

Gene position: 247700-248710 (Clockwise)

Preceding gene: 21240974

Following gene: 21240978

Centisome position: 4.79

GC content: 67.46

Gene sequence:

>1011_bases
TTGAAAGCATTCCAGATTGGCAATCAAACCGGCCTTGAGAGCCTGGTTGCGGTCGAGATGCCCGAACCAGTTGCAGGAGC
CGGTGAGGTCATCGTCGCTCCGCGACTGGTCGGGCTGATCAACCGCGACCTGCAGATCCTGAAAGGCACCTACGGCGGCA
AGCTTGCGCCTACGCGCGTTCCGGTTGCCGAGGGTGTGGGCATCGTGACCTGCGTCGGTGCCGGCGTCGGCCAGGTCGTG
CCAGGCGACCGTGTCGTCAGTGGGCACTTTCCAACCTGGCTGGATGGCAGGTTCCGCGCCGAGATGTTCGCCCACGACCT
GGGCGTGACCCATGACGGCTGGCTGGCCGAAAAGGTGTCGCTGCCCGCCAGCGCCCTGATCAAGGTGCCTGCGTCGCTGG
AGGACGCGGATGTCGCTGCGCTGGCATCGGCCGCGGTGACCGCCTGGCACGCCCTGGTGGAAGTGGCCAGGATCAAGGCC
GGCGACCTGGTGCTGTGTCTGGGAACCGGTGGCGTCTCGATGGCCGCGCTCCAGCTGGCCAAGCTCCACGGCGCCCGCGT
CGCCATCACCTCGTCCAGCGACGACAAGCTCGAGCTCGCACGCCGCCTGGGCGCCGACATCACCGTCAACTACCGCACCC
ACCCCGACTGGGCCGAGGCGGTGATGCAGCTGTCCGGCGGCGGCGCGGACGTCGTCATCGAGACCGGCGGGCAGGACACC
TTGGGCCAGTCGATCAAGGCGGCGGCCGCGAACGCGACCATCGTGGTGGTCGGCGTCACCCCGGGCGCGGCGTCGCCAAT
TCCCGATTACGTGGCGTTGATCCGCAAGAACGTGACCATCCGCGGGATCGCCAACGGAAGCCGGGCGATGTTCGTGGACC
TGCTGCGGGCGATCGAGCAGCACCGCATCAAGACCCTGCACAGCAAGACGTTCCGGTTCGACGAGGCGCCCCAGGCCTTG
GACTATTTCGCCAGGGCGCAACACGTGGGCAAGGTCCTCATCCGGTTGTGA

Upstream 100 bases:

>100_bases
ACCCATCGCTGCGCTGAGTGATCGCGCCGATGGCGGCGGTCGCTCCATCCTCCATGACGCCAGCCACGTTCGGCCGGCGC
CGCAGCACAGGCAATCCCCC

Downstream 100 bases:

>100_bases
TGCGGGGCGGCGGCGGCGCGTGCGAGGCCGTTGCCGGCCCAATGCTGCGGCCGCGCTAGCTGCCGGCCTCGATCATCTGG
TCGAGCACGTACACCGGTTT

Product: alcohol dehydrogenase

Products: Glycerol; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKAFQIGNQTGLESLVAVEMPEPVAGAGEVIVAPRLVGLINRDLQILKGTYGGKLAPTRVPVAEGVGIVTCVGAGVGQVV
PGDRVVSGHFPTWLDGRFRAEMFAHDLGVTHDGWLAEKVSLPASALIKVPASLEDADVAALASAAVTAWHALVEVARIKA
GDLVLCLGTGGVSMAALQLAKLHGARVAITSSSDDKLELARRLGADITVNYRTHPDWAEAVMQLSGGGADVVIETGGQDT
LGQSIKAAAANATIVVVGVTPGAASPIPDYVALIRKNVTIRGIANGSRAMFVDLLRAIEQHRIKTLHSKTFRFDEAPQAL
DYFARAQHVGKVLIRL

Sequences:

>Translated_336_residues
MKAFQIGNQTGLESLVAVEMPEPVAGAGEVIVAPRLVGLINRDLQILKGTYGGKLAPTRVPVAEGVGIVTCVGAGVGQVV
PGDRVVSGHFPTWLDGRFRAEMFAHDLGVTHDGWLAEKVSLPASALIKVPASLEDADVAALASAAVTAWHALVEVARIKA
GDLVLCLGTGGVSMAALQLAKLHGARVAITSSSDDKLELARRLGADITVNYRTHPDWAEAVMQLSGGGADVVIETGGQDT
LGQSIKAAAANATIVVVGVTPGAASPIPDYVALIRKNVTIRGIANGSRAMFVDLLRAIEQHRIKTLHSKTFRFDEAPQAL
DYFARAQHVGKVLIRL
>Mature_336_residues
MKAFQIGNQTGLESLVAVEMPEPVAGAGEVIVAPRLVGLINRDLQILKGTYGGKLAPTRVPVAEGVGIVTCVGAGVGQVV
PGDRVVSGHFPTWLDGRFRAEMFAHDLGVTHDGWLAEKVSLPASALIKVPASLEDADVAALASAAVTAWHALVEVARIKA
GDLVLCLGTGGVSMAALQLAKLHGARVAITSSSDDKLELARRLGADITVNYRTHPDWAEAVMQLSGGGADVVIETGGQDT
LGQSIKAAAANATIVVVGVTPGAASPIPDYVALIRKNVTIRGIANGSRAMFVDLLRAIEQHRIKTLHSKTFRFDEAPQAL
DYFARAQHVGKVLIRL

Specific function: Preferred Specificity Is Towards 1-Propanol. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=351, Percent_Identity=25.3561253561254, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI22538444, Length=351, Percent_Identity=25.3561253561254, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI18379349, Length=326, Percent_Identity=25.4601226993865, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI28557745, Length=349, Percent_Identity=24.9283667621776, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI87081918, Length=281, Percent_Identity=29.5373665480427, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790045, Length=337, Percent_Identity=27.0029673590504, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790485, Length=260, Percent_Identity=27.3076923076923, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787863, Length=352, Percent_Identity=26.1363636363636, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=270, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324486, Length=332, Percent_Identity=26.5060240963855, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=332, Percent_Identity=25.9036144578313, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323729, Length=332, Percent_Identity=25.3012048192771, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6319621, Length=331, Percent_Identity=24.1691842900302, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319520, Length=278, Percent_Identity=24.4604316546763, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.1 [C]

Molecular weight: Translated: 35144; Mature: 35144

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFQIGNQTGLESLVAVEMPEPVAGAGEVIVAPRLVGLINRDLQILKGTYGGKLAPTRV
CCCEECCCCCCCHHEEEEECCCCCCCCCCEEEHHHHHHHHHCCHHEEECCCCCCCCCCCC
PVAEGVGIVTCVGAGVGQVVPGDRVVSGHFPTWLDGRFRAEMFAHDLGVTHDGWLAEKVS
CHHHCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHC
LPASALIKVPASLEDADVAALASAAVTAWHALVEVARIKAGDLVLCLGTGGVSMAALQLA
CCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
KLHGARVAITSSSDDKLELARRLGADITVNYRTHPDWAEAVMQLSGGGADVVIETGGQDT
HHCCCEEEEECCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCHH
LGQSIKAAAANATIVVVGVTPGAASPIPDYVALIRKNVTIRGIANGSRAMFVDLLRAIEQ
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHH
HRIKTLHSKTFRFDEAPQALDYFARAQHVGKVLIRL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKAFQIGNQTGLESLVAVEMPEPVAGAGEVIVAPRLVGLINRDLQILKGTYGGKLAPTRV
CCCEECCCCCCCHHEEEEECCCCCCCCCCEEEHHHHHHHHHCCHHEEECCCCCCCCCCCC
PVAEGVGIVTCVGAGVGQVVPGDRVVSGHFPTWLDGRFRAEMFAHDLGVTHDGWLAEKVS
CHHHCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHC
LPASALIKVPASLEDADVAALASAAVTAWHALVEVARIKAGDLVLCLGTGGVSMAALQLA
CCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
KLHGARVAITSSSDDKLELARRLGADITVNYRTHPDWAEAVMQLSGGGADVVIETGGQDT
HHCCCEEEEECCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCHH
LGQSIKAAAANATIVVVGVTPGAASPIPDYVALIRKNVTIRGIANGSRAMFVDLLRAIEQ
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHH
HRIKTLHSKTFRFDEAPQALDYFARAQHVGKVLIRL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.004 {benzyl} 0.005 {cyclohe

Substrates: D-Glyceraldehyde; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: D-Glyceraldehyde + H+ + Nicotinamide adenine dinucleotide - reduced <==> Glycerol + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]