Click here to switch to the map view.

The map label for this gene is glnA [H]

Identifier: 21240978

GI number: 21240978

Start: 250876

End: 252285

Strand: Direct

Name: glnA [H]

Synonym: XAC0204

Alternate gene names: 21240978

Gene position: 250876-252285 (Clockwise)

Preceding gene: 21240975

Following gene: 21240979

Centisome position: 4.85

GC content: 62.27

Gene sequence:

>1410_bases
ATGTCCGTGGAAAATGTTGAAAAGCTGATCAAGGACAACAAGGTCGAGTTCGTCGACCTGCGCTTCGTCGATATGCGTGG
TGTACAGCAGCACATCACCTTTCCGGTCAATATCATCGAGCCGGCGCTGTTTGAAGAAGGCAAGATGTTCGACGGCAGTT
CGATCGCGGGCTGGAAGGGCATCAACGAGTCGGACATGGTGTTGCTGCCGGACGCGGGCACCGCGTATCTGGATCCGTTC
TTCGCCGATCCGACCATCGTGCTGACCTGCGACATCCTCGACCCGGCCACCATGCAGAGCTATGAGCGCGATCCGCGCGG
CATCGCCAAGCGCGCCGAGGCCTATCTGAAATCCTCCGGCACCGCCGACCAGGCGTTCTTCGGCCCGGAGCCGGAATTCT
TCATCTTCGATTCGGTGCGCTTTGCCAACGACATGGGCCACACCTTCTTCCAGGTGGGTTCGGAAGAAGCGGCCTGGAAC
ACCGGCGAAAAGTACGACGGCGGCAACAGCGGTTACCGCCCGGGCGTGAAGGGCGGCTACTTCCCGGTTCCGCCGACCGA
CAGCCTGCACGACCTGCGCGCGGAAATGATCAAGACGCTGGAACAGGTCGGCATCGAAACCGAAGTGCACCACCACGAAG
TGGCCACCGCCGGCCAGTGCGAGATCGGCACCAAGTTCAGCTCGCTGGTGCAGAAGGCCGACGAACTGCTGACGATGAAG
TACATCATCAAGAACGTCGCCTACCGCAACGGCAAGACCGCCACCTTCATGCCCAAGCCCATCGTGGGCGACAACGGCTC
GGGCATGCACGTGCACCAGTCGCTGGCCAAGGGCGGCGTCAACCTGTTCACCGGCGACGGCTACGGCGGCCTGTCGCAGC
TGGCGCTGTGGTACATCGGCGGCATCTTCAAGCATGCCCGCGCGATCAACGCCTTCGCCAACTCCGGCACCAACAGCTAC
AAGCGTCTGGTCCCGGGCTTCGAGGCGCCGGTGATGCTGGCCTATTCGGCGCGTAACCGTTCGGCGTCGTGCCGCATTCC
CTGGGTCACCAATCCGAAGGCGCGCCGCATCGAAATGCGCTTCCCCGATCCGTTGCAGTCCGGCTACCTGACCTTCACCG
CGCTGATGATGGCCGGCCTGGACGGCATCAAGAACCAGATCGATCCGGGCGCACCGAGCGACAAGGACCTGTACGACCTG
CCGCCGGAAGAAGAGAAGCTGATCCCGCAGGTTTGCTCCAGCCTGGACCAGGCGCTGGAAGCGCTGGACAAGGACCGCGA
GTTCCTCAAGGCCGGCGGCGTGATGAGCGACGACTTCATCGACGGCTATATCGCGCTGAAGATGCAGGAAGTCACCAAGT
TCCGTGCTGCGACCCACCCGTTGGAGTACCAGCTGTACTACGGCAACTGA

Upstream 100 bases:

>100_bases
TGTAAGCAAAATCAAGGGCTTGTGAAGTGCCAGGGCTTGGCACGGTCCCTGCTGTAGTCCCGCCCACGAGCCACTTAACT
CCTGCCTGAGGTTTTTTCCG

Downstream 100 bases:

>100_bases
GTTCCACCGCGGTGATGGCGGAGGCCCCCCTCCCACCTCCGCCATCGCCGCACACTTGTTCGAGCGATCCGTGGCCCCGG
CCGTGGATCGCAGGGGTATT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSVENVEKLIKDNKVEFVDLRFVDMRGVQQHITFPVNIIEPALFEEGKMFDGSSIAGWKGINESDMVLLPDAGTAYLDPF
FADPTIVLTCDILDPATMQSYERDPRGIAKRAEAYLKSSGTADQAFFGPEPEFFIFDSVRFANDMGHTFFQVGSEEAAWN
TGEKYDGGNSGYRPGVKGGYFPVPPTDSLHDLRAEMIKTLEQVGIETEVHHHEVATAGQCEIGTKFSSLVQKADELLTMK
YIIKNVAYRNGKTATFMPKPIVGDNGSGMHVHQSLAKGGVNLFTGDGYGGLSQLALWYIGGIFKHARAINAFANSGTNSY
KRLVPGFEAPVMLAYSARNRSASCRIPWVTNPKARRIEMRFPDPLQSGYLTFTALMMAGLDGIKNQIDPGAPSDKDLYDL
PPEEEKLIPQVCSSLDQALEALDKDREFLKAGGVMSDDFIDGYIALKMQEVTKFRAATHPLEYQLYYGN

Sequences:

>Translated_469_residues
MSVENVEKLIKDNKVEFVDLRFVDMRGVQQHITFPVNIIEPALFEEGKMFDGSSIAGWKGINESDMVLLPDAGTAYLDPF
FADPTIVLTCDILDPATMQSYERDPRGIAKRAEAYLKSSGTADQAFFGPEPEFFIFDSVRFANDMGHTFFQVGSEEAAWN
TGEKYDGGNSGYRPGVKGGYFPVPPTDSLHDLRAEMIKTLEQVGIETEVHHHEVATAGQCEIGTKFSSLVQKADELLTMK
YIIKNVAYRNGKTATFMPKPIVGDNGSGMHVHQSLAKGGVNLFTGDGYGGLSQLALWYIGGIFKHARAINAFANSGTNSY
KRLVPGFEAPVMLAYSARNRSASCRIPWVTNPKARRIEMRFPDPLQSGYLTFTALMMAGLDGIKNQIDPGAPSDKDLYDL
PPEEEKLIPQVCSSLDQALEALDKDREFLKAGGVMSDDFIDGYIALKMQEVTKFRAATHPLEYQLYYGN
>Mature_468_residues
SVENVEKLIKDNKVEFVDLRFVDMRGVQQHITFPVNIIEPALFEEGKMFDGSSIAGWKGINESDMVLLPDAGTAYLDPFF
ADPTIVLTCDILDPATMQSYERDPRGIAKRAEAYLKSSGTADQAFFGPEPEFFIFDSVRFANDMGHTFFQVGSEEAAWNT
GEKYDGGNSGYRPGVKGGYFPVPPTDSLHDLRAEMIKTLEQVGIETEVHHHEVATAGQCEIGTKFSSLVQKADELLTMKY
IIKNVAYRNGKTATFMPKPIVGDNGSGMHVHQSLAKGGVNLFTGDGYGGLSQLALWYIGGIFKHARAINAFANSGTNSYK
RLVPGFEAPVMLAYSARNRSASCRIPWVTNPKARRIEMRFPDPLQSGYLTFTALMMAGLDGIKNQIDPGAPSDKDLYDLP
PEEEKLIPQVCSSLDQALEALDKDREFLKAGGVMSDDFIDGYIALKMQEVTKFRAATHPLEYQLYYGN

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=467, Percent_Identity=63.3832976445396, Blast_Score=648, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=391, Percent_Identity=23.5294117647059, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51846; Mature: 51715

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVENVEKLIKDNKVEFVDLRFVDMRGVQQHITFPVNIIEPALFEEGKMFDGSSIAGWKG
CCHHHHHHHHHCCCEEEEEEEEECHHCHHHHCCCCHHHHCHHHHCCCCCCCCCCCCCCCC
INESDMVLLPDAGTAYLDPFFADPTIVLTCDILDPATMQSYERDPRGIAKRAEAYLKSSG
CCCCCEEEECCCCCHHCCCCCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHCCC
TADQAFFGPEPEFFIFDSVRFANDMGHTFFQVGSEEAAWNTGEKYDGGNSGYRPGVKGGY
CCCCCCCCCCCCEEEEECHHHHHHHCCHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCC
FPVPPTDSLHDLRAEMIKTLEQVGIETEVHHHEVATAGQCEIGTKFSSLVQKADELLTMK
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
YIIKNVAYRNGKTATFMPKPIVGDNGSGMHVHQSLAKGGVNLFTGDGYGGLSQLALWYIG
HHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHH
GIFKHARAINAFANSGTNSYKRLVPGFEAPVMLAYSARNRSASCRIPWVTNPKARRIEMR
HHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCCCEEECCCCCCCCCEEEEC
FPDPLQSGYLTFTALMMAGLDGIKNQIDPGAPSDKDLYDLPPEEEKLIPQVCSSLDQALE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ALDKDREFLKAGGVMSDDFIDGYIALKMQEVTKFRAATHPLEYQLYYGN
HHHHHHHHHHHCCCCCCHHCCCEEEEEHHHHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure 
SVENVEKLIKDNKVEFVDLRFVDMRGVQQHITFPVNIIEPALFEEGKMFDGSSIAGWKG
CHHHHHHHHHCCCEEEEEEEEECHHCHHHHCCCCHHHHCHHHHCCCCCCCCCCCCCCCC
INESDMVLLPDAGTAYLDPFFADPTIVLTCDILDPATMQSYERDPRGIAKRAEAYLKSSG
CCCCCEEEECCCCCHHCCCCCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHCCC
TADQAFFGPEPEFFIFDSVRFANDMGHTFFQVGSEEAAWNTGEKYDGGNSGYRPGVKGGY
CCCCCCCCCCCCEEEEECHHHHHHHCCHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCC
FPVPPTDSLHDLRAEMIKTLEQVGIETEVHHHEVATAGQCEIGTKFSSLVQKADELLTMK
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
YIIKNVAYRNGKTATFMPKPIVGDNGSGMHVHQSLAKGGVNLFTGDGYGGLSQLALWYIG
HHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHH
GIFKHARAINAFANSGTNSYKRLVPGFEAPVMLAYSARNRSASCRIPWVTNPKARRIEMR
HHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCCCEEECCCCCCCCCEEEEC
FPDPLQSGYLTFTALMMAGLDGIKNQIDPGAPSDKDLYDLPPEEEKLIPQVCSSLDQALE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ALDKDREFLKAGGVMSDDFIDGYIALKMQEVTKFRAATHPLEYQLYYGN
HHHHHHHHHHHCCCCCCHHCCCEEEEEHHHHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]