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The map label for this gene is cimA [H]

Identifier: 21226773

GI number: 21226773

Start: 805009

End: 806460

Strand: Reverse

Name: cimA [H]

Synonym: MM_0671

Alternate gene names: 21226773

Gene position: 806460-805009 (Counterclockwise)

Preceding gene: 21226774

Following gene: 21226772

Centisome position: 19.69

GC content: 48.97

Gene sequence:

>1452_bases
TTGAGAGATGGTGAACAAACACCTGGCGTAGCCCTGACGAAAGAGAAAAAGCTCTTAATTGCAAGAGCCCTTGATGAGAT
GAAAATCAATGTAATCGAGGCAGGCTCTGCAATTACTTCCGCAGGCGAAAGGGAATCAGTCAGGGCTGTAGCGAATGCAG
GGCTCAGTGCCGAAATCTGCAGCTACTGCAGGATTGTAAAAACCGACGTTGACCATGCTCTTGAGTGCGATGTTGACTCT
ATCCACCTTGTCGTTCCGGTTTCTGACCTGCATATCAGGACGAAAATCAAAAAAGACCGGGATACAGTAAGGCAGATTGC
AGCCGATGTAACCGAGTATGCCAAAGAACACGGTCTGATTGTGGAACTTAGCGGTGAGGATTCTTCGAGGGCAGACCTTG
GCTTCTTAAAAGCCGTTTACTCGGACGGAATAGACGCCGGAGCTGACAGATTATGCTTCTGCGATACGGTAGGTCTTCTG
GTGCCCGAAAAAACCACTGAAATCTTCCGTGACCTGTCTTCTTCCCTGAAAGCCCCTATCAGCATTCACTGTCACAACGA
CTTTGGTTTAGCAACTGCAAATACGGTCGCAGCGCTCGCGGCAGGAGCAAAACAGGCTCATGTAACAATTAACGGTTTAG
GTGAAAGAGCAGGAAATGCCTCTCTTGAAGAAGTCGTAATGTGCCTTGAGTGGCTGTACAAATATGACACCGGCATTAAG
CACGAGCAGATATACCGGACTTCGAGGCTTGTGAGCCGCCTTACCGGAATTCCGGTTTCTCCCAATAAAGCCCTTGTGGG
AGGAAATGCCTTCACCCATGAAGCAGGCATTCATGTGCACGGGCTTCTGGCTGACAAGGCAACCTATGAGCCAATGAGCC
CTGAATATATTGGACGGCAGCGCCAGATCGTACTTGGAAAACACGCGGGAAGAAGCTCTATTACCCTTGCTTTAAAGGAA
ATGGGGCTTGAAGCTGATGATGCCCAGACTGAAGAGATTTTCAACAGGGTCAAGCAGATGGGCGACCAGGGTAAACATGT
TACAGATGCTGACCTGCAGATCATCGCAGAAACCGTGCTTGATATCTACAAAGAACCTCTGGTAAAGTTAGAAGAATTCA
CCATAGTATCCGGAAACCGGGTGACTCCAACAGCTTCCATAAAGCTCAATGTAAAAGACAAAGAGATCGTACAGGCCGGA
ATCGGAAACGGACCTGTGGATGCGGTTATCAATGCTATCCGGAGAGCTGTAAGTTCCTGTGCTGAAGACGTCATCCTTGA
AGAGTACCATGTAGACGCGGTTACCGGCGGAACCGATGCCCTTGTAGAGGTAAGGGTAAAGCTTTCCAAGGAAGGAAAGT
CAATCACCGCAAGTGGAGCCAGGACGGACATTATCATGGCTTCTGTAGAAGCCGTAATGAACGGACTTAACCGCCTTGTC
CGGGCAGAATAA

Upstream 100 bases:

>100_bases
GTAATACTATATTTCCTGTTCTGCAGAACCAATCTATCCAAATAAAAACCAATACTGAGGGATTATATTATTTGAAAACA
TCCGTTTTCTGGATACTACT

Downstream 100 bases:

>100_bases
GTCACAAAAAAGCATGGAACTTCAAAGGTTTTTCAGAGGACTTTTTGTCCTCTCTTTTTTTCTGTTTGAAGAAATTTTTG
AAATCGGGGTCTCTGGTACT

Product: (R)-citramalate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MRDGEQTPGVALTKEKKLLIARALDEMKINVIEAGSAITSAGERESVRAVANAGLSAEICSYCRIVKTDVDHALECDVDS
IHLVVPVSDLHIRTKIKKDRDTVRQIAADVTEYAKEHGLIVELSGEDSSRADLGFLKAVYSDGIDAGADRLCFCDTVGLL
VPEKTTEIFRDLSSSLKAPISIHCHNDFGLATANTVAALAAGAKQAHVTINGLGERAGNASLEEVVMCLEWLYKYDTGIK
HEQIYRTSRLVSRLTGIPVSPNKALVGGNAFTHEAGIHVHGLLADKATYEPMSPEYIGRQRQIVLGKHAGRSSITLALKE
MGLEADDAQTEEIFNRVKQMGDQGKHVTDADLQIIAETVLDIYKEPLVKLEEFTIVSGNRVTPTASIKLNVKDKEIVQAG
IGNGPVDAVINAIRRAVSSCAEDVILEEYHVDAVTGGTDALVEVRVKLSKEGKSITASGARTDIIMASVEAVMNGLNRLV
RAE

Sequences:

>Translated_483_residues
MRDGEQTPGVALTKEKKLLIARALDEMKINVIEAGSAITSAGERESVRAVANAGLSAEICSYCRIVKTDVDHALECDVDS
IHLVVPVSDLHIRTKIKKDRDTVRQIAADVTEYAKEHGLIVELSGEDSSRADLGFLKAVYSDGIDAGADRLCFCDTVGLL
VPEKTTEIFRDLSSSLKAPISIHCHNDFGLATANTVAALAAGAKQAHVTINGLGERAGNASLEEVVMCLEWLYKYDTGIK
HEQIYRTSRLVSRLTGIPVSPNKALVGGNAFTHEAGIHVHGLLADKATYEPMSPEYIGRQRQIVLGKHAGRSSITLALKE
MGLEADDAQTEEIFNRVKQMGDQGKHVTDADLQIIAETVLDIYKEPLVKLEEFTIVSGNRVTPTASIKLNVKDKEIVQAG
IGNGPVDAVINAIRRAVSSCAEDVILEEYHVDAVTGGTDALVEVRVKLSKEGKSITASGARTDIIMASVEAVMNGLNRLV
RAE
>Mature_483_residues
MRDGEQTPGVALTKEKKLLIARALDEMKINVIEAGSAITSAGERESVRAVANAGLSAEICSYCRIVKTDVDHALECDVDS
IHLVVPVSDLHIRTKIKKDRDTVRQIAADVTEYAKEHGLIVELSGEDSSRADLGFLKAVYSDGIDAGADRLCFCDTVGLL
VPEKTTEIFRDLSSSLKAPISIHCHNDFGLATANTVAALAAGAKQAHVTINGLGERAGNASLEEVVMCLEWLYKYDTGIK
HEQIYRTSRLVSRLTGIPVSPNKALVGGNAFTHEAGIHVHGLLADKATYEPMSPEYIGRQRQIVLGKHAGRSSITLALKE
MGLEADDAQTEEIFNRVKQMGDQGKHVTDADLQIIAETVLDIYKEPLVKLEEFTIVSGNRVTPTASIKLNVKDKEIVQAG
IGNGPVDAVINAIRRAVSSCAEDVILEEYHVDAVTGGTDALVEVRVKLSKEGKSITASGARTDIIMASVEAVMNGLNRLV
RAE

Specific function: Catalyzes the condensation of pyruvate and acetyl- coenzyme A to form (R)-citramalate [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=494, Percent_Identity=35.4251012145749, Blast_Score=284, Evalue=7e-78,
Organism=Saccharomyces cerevisiae, GI6320019, Length=345, Percent_Identity=32.1739130434783, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6320071, Length=349, Percent_Identity=32.378223495702, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6324682, Length=466, Percent_Identity=21.8884120171674, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.1.182 [H]

Molecular weight: Translated: 52041; Mature: 52041

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDGEQTPGVALTKEKKLLIARALDEMKINVIEAGSAITSAGERESVRAVANAGLSAEIC
CCCCCCCCCEEEECCHHHHHHHHHHHHEEEEEECCCHHHCCCCHHHHHHHHHCCCCHHHH
SYCRIVKTDVDHALECDVDSIHLVVPVSDLHIRTKIKKDRDTVRQIAADVTEYAKEHGLI
HHHHHHHHCCCHHHCCCCCCEEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEE
VELSGEDSSRADLGFLKAVYSDGIDAGADRLCFCDTVGLLVPEKTTEIFRDLSSSLKAPI
EEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECHHCCCCCCHHHHHHHHHHHHCCCCE
SIHCHNDFGLATANTVAALAAGAKQAHVTINGLGERAGNASLEEVVMCLEWLYKYDTGIK
EEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCC
HEQIYRTSRLVSRLTGIPVSPNKALVGGNAFTHEAGIHVHGLLADKATYEPMSPEYIGRQ
HHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEECCCCEEEEEEECCCCCCCCCHHHCCCC
RQIVLGKHAGRSSITLALKEMGLEADDAQTEEIFNRVKQMGDQGKHVTDADLQIIAETVL
CEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
DIYKEPLVKLEEFTIVSGNRVTPTASIKLNVKDKEIVQAGIGNGPVDAVINAIRRAVSSC
HHHHHHHHEEHHEEEECCCEECCEEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AEDVILEEYHVDAVTGGTDALVEVRVKLSKEGKSITASGARTDIIMASVEAVMNGLNRLV
HHHHHHHHHEEEEECCCCHHEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
RAE
CCC
>Mature Secondary Structure
MRDGEQTPGVALTKEKKLLIARALDEMKINVIEAGSAITSAGERESVRAVANAGLSAEIC
CCCCCCCCCEEEECCHHHHHHHHHHHHEEEEEECCCHHHCCCCHHHHHHHHHCCCCHHHH
SYCRIVKTDVDHALECDVDSIHLVVPVSDLHIRTKIKKDRDTVRQIAADVTEYAKEHGLI
HHHHHHHHCCCHHHCCCCCCEEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEE
VELSGEDSSRADLGFLKAVYSDGIDAGADRLCFCDTVGLLVPEKTTEIFRDLSSSLKAPI
EEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECHHCCCCCCHHHHHHHHHHHHCCCCE
SIHCHNDFGLATANTVAALAAGAKQAHVTINGLGERAGNASLEEVVMCLEWLYKYDTGIK
EEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCC
HEQIYRTSRLVSRLTGIPVSPNKALVGGNAFTHEAGIHVHGLLADKATYEPMSPEYIGRQ
HHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEECCCCEEEEEEECCCCCCCCCHHHCCCC
RQIVLGKHAGRSSITLALKEMGLEADDAQTEEIFNRVKQMGDQGKHVTDADLQIIAETVL
CEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
DIYKEPLVKLEEFTIVSGNRVTPTASIKLNVKDKEIVQAGIGNGPVDAVINAIRRAVSSC
HHHHHHHHEEHHEEEECCCEECCEEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AEDVILEEYHVDAVTGGTDALVEVRVKLSKEGKSITASGARTDIIMASVEAVMNGLNRLV
HHHHHHHHHEEEEECCCCHHEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
RAE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]