Click here to switch to the map view.

The map label for this gene is ilvB [H]

Identifier: 21226772

GI number: 21226772

Start: 803011

End: 804705

Strand: Reverse

Name: ilvB [H]

Synonym: MM_0670

Alternate gene names: 21226772

Gene position: 804705-803011 (Counterclockwise)

Preceding gene: 21226773

Following gene: 21226771

Centisome position: 19.64

GC content: 49.44

Gene sequence:

>1695_bases
ATGACAACCGGGTCAACAGACAAAGTCAACGGCGCAAAGGCTCTTATAAAGTGCCTCGAAAAAGAAGGAGTTGACACTCT
CTTCGGATACCCCGGAGGGCAGATCATTCCTTTCTACAATGAACTCTATGATTCAGATCTTCGCCACATACTTGTACGCC
ACGAACAGGCAGCTGCTCATGCTGCGGACGGGTACGCCCGTGCTACTGGAAAAACCGGGGTCTGCGTATCCACTTCAGGT
CCCGGAGCAACCAACCTGGTAACAGGTATTGCTACTGCATACATGGATTCTGTGCCGATTGTAGCATTAACCGGCCAGGT
GCCGAGGTCCCTTATCGGAAACGATGCTTTCCAGGAAGCGGATATTACAGGCATTACCATGCCGATTACCAAGCACAACT
ATCTCGTACAGGATCCGCAAGAAATCCCGAGGATTGTAAAAGAAGCCTTCCACATAGCGTCTACCGGAAGGCCTGGCCCG
GTATTAATTGATCTCCCGAAGGATGTACAGAATATAGAGATTGACTTACACTATCCTGAGAGGGTAGAGCTCAGGGGCTA
TAAGCCTACTTATAAAGGAAACACTCAGCAGATTAAACGAGCCGCTGAAGAGATTGCAAACTCCTGCAGGCCTATAATCT
ACGCAGGTGGCGGAGTGATAAGTTCCAATGCAAGTGCAGAGCTTGTTGAGTTTGCCGAAACTATCAAGGCTCCCGTCACA
ACCACACTTATGGGTATAAGTTCTATCCCGACTGAACATCCCCTGTATGTCGGAATGCTGGGGATGCACGGGTGTAAGTA
CGCAAACTATGCGATTCAGGAATCCGACCTGATTATTGCTGTGGGAGCAAGATTTGACGACCGTGTTACCGGCAAACTCG
AATCCTTTGCCCCCAACGCCAGAGTTATTCATATTGATGTTGACCCTGCAGAGATTTCCAAGAATGTAAAGGTACATATC
CCCATTGTCGGGGATGCAAAACAGGTTCTCAAATCCCTGATCAGATATGTCCAGTGCTGCAGGTCAGCAGAATGGATTGA
AAAGGTAAACCTGTGGAAGAAAGAATATCCTCTCAACTACAGGGAGTGCAGGGATACTATAATGCCCCAATTTGTTATCG
AACAGATCTCCGAAGTCTGCAGGGATGCAATTATAGTTACCGAGGTCGGACAGCACCAGATGTGGGCTGCCCAGTTCTTC
AAATACAGCAAACCCAGGACTTTCCTCACTTCTGGCGGACTCGGCACAATGGGATACGGGTTCCCGGCAGCCATGGGGGC
CAAGGTGGGCAGGCCGGACAAAACCGTTATCAATATCGCAGGGGACGGCTCTTTCCAGATGAACTCCCAGGAGCTTGCAA
CTGTGGTCCAGAACGATATCCCTGTTGTTTCCGTGATCCTGAACAACGGCTATCTGGGCATGGTCAGGCAGTGGCAGGAA
CTCTTCTATGACAGGAGGTATTCCTACACATTCATTAAAGGCAGTGTTGACTTTGTAAAGCTTGCTGAAGCCTATGGAGC
CCTTGGACTTCGTGCAGAGAGGCCGTCTGAAGTCAGGCCTGCAATCGAAGAGGCTGTTAATTCCGGAAGGCCGACAGTTG
TTGAAGTTATTGTCGAGTGCGAGGCAAATGTTTATCCAATGGTTCCGGCAGGGGCTGCAATTAATGAGATCATTGACCTG
GAGGAACACAGATGA

Upstream 100 bases:

>100_bases
AATATAGTATTTCTTATTACACACCCTGACTGGAAAGGCTCACCTGTAATGTGAGCGCCCAGCCGGACAAAATCTCAAGA
AATAAGTAAGGAGATAAATA

Downstream 100 bases:

>100_bases
AACATACACTTGCAGTCCTTGTGGAAAATAAGTCGGGTGTGCTCTCCAGAGTTGCTTCTCTCTTTTCAAGGCGTGGATAC
AATATCGACAGCCTTGCAGT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MTTGSTDKVNGAKALIKCLEKEGVDTLFGYPGGQIIPFYNELYDSDLRHILVRHEQAAAHAADGYARATGKTGVCVSTSG
PGATNLVTGIATAYMDSVPIVALTGQVPRSLIGNDAFQEADITGITMPITKHNYLVQDPQEIPRIVKEAFHIASTGRPGP
VLIDLPKDVQNIEIDLHYPERVELRGYKPTYKGNTQQIKRAAEEIANSCRPIIYAGGGVISSNASAELVEFAETIKAPVT
TTLMGISSIPTEHPLYVGMLGMHGCKYANYAIQESDLIIAVGARFDDRVTGKLESFAPNARVIHIDVDPAEISKNVKVHI
PIVGDAKQVLKSLIRYVQCCRSAEWIEKVNLWKKEYPLNYRECRDTIMPQFVIEQISEVCRDAIIVTEVGQHQMWAAQFF
KYSKPRTFLTSGGLGTMGYGFPAAMGAKVGRPDKTVINIAGDGSFQMNSQELATVVQNDIPVVSVILNNGYLGMVRQWQE
LFYDRRYSYTFIKGSVDFVKLAEAYGALGLRAERPSEVRPAIEEAVNSGRPTVVEVIVECEANVYPMVPAGAAINEIIDL
EEHR

Sequences:

>Translated_564_residues
MTTGSTDKVNGAKALIKCLEKEGVDTLFGYPGGQIIPFYNELYDSDLRHILVRHEQAAAHAADGYARATGKTGVCVSTSG
PGATNLVTGIATAYMDSVPIVALTGQVPRSLIGNDAFQEADITGITMPITKHNYLVQDPQEIPRIVKEAFHIASTGRPGP
VLIDLPKDVQNIEIDLHYPERVELRGYKPTYKGNTQQIKRAAEEIANSCRPIIYAGGGVISSNASAELVEFAETIKAPVT
TTLMGISSIPTEHPLYVGMLGMHGCKYANYAIQESDLIIAVGARFDDRVTGKLESFAPNARVIHIDVDPAEISKNVKVHI
PIVGDAKQVLKSLIRYVQCCRSAEWIEKVNLWKKEYPLNYRECRDTIMPQFVIEQISEVCRDAIIVTEVGQHQMWAAQFF
KYSKPRTFLTSGGLGTMGYGFPAAMGAKVGRPDKTVINIAGDGSFQMNSQELATVVQNDIPVVSVILNNGYLGMVRQWQE
LFYDRRYSYTFIKGSVDFVKLAEAYGALGLRAERPSEVRPAIEEAVNSGRPTVVEVIVECEANVYPMVPAGAAINEIIDL
EEHR
>Mature_563_residues
TTGSTDKVNGAKALIKCLEKEGVDTLFGYPGGQIIPFYNELYDSDLRHILVRHEQAAAHAADGYARATGKTGVCVSTSGP
GATNLVTGIATAYMDSVPIVALTGQVPRSLIGNDAFQEADITGITMPITKHNYLVQDPQEIPRIVKEAFHIASTGRPGPV
LIDLPKDVQNIEIDLHYPERVELRGYKPTYKGNTQQIKRAAEEIANSCRPIIYAGGGVISSNASAELVEFAETIKAPVTT
TLMGISSIPTEHPLYVGMLGMHGCKYANYAIQESDLIIAVGARFDDRVTGKLESFAPNARVIHIDVDPAEISKNVKVHIP
IVGDAKQVLKSLIRYVQCCRSAEWIEKVNLWKKEYPLNYRECRDTIMPQFVIEQISEVCRDAIIVTEVGQHQMWAAQFFK
YSKPRTFLTSGGLGTMGYGFPAAMGAKVGRPDKTVINIAGDGSFQMNSQELATVVQNDIPVVSVILNNGYLGMVRQWQEL
FYDRRYSYTFIKGSVDFVKLAEAYGALGLRAERPSEVRPAIEEAVNSGRPTVVEVIVECEANVYPMVPAGAAINEIIDLE
EHR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=569, Percent_Identity=24.9560632688928, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI21361361, Length=577, Percent_Identity=25.3032928942808, Blast_Score=159, Evalue=8e-39,
Organism=Escherichia coli, GI87081685, Length=565, Percent_Identity=46.1946902654867, Blast_Score=543, Evalue=1e-155,
Organism=Escherichia coli, GI1790104, Length=559, Percent_Identity=44.3649373881932, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1786717, Length=580, Percent_Identity=37.0689655172414, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1787096, Length=560, Percent_Identity=29.6428571428571, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1788716, Length=559, Percent_Identity=25.7602862254025, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17531301, Length=569, Percent_Identity=26.3620386643234, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17531299, Length=569, Percent_Identity=26.3620386643234, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17542570, Length=601, Percent_Identity=23.1281198003328, Blast_Score=138, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=45.53264604811, Blast_Score=491, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6321524, Length=524, Percent_Identity=26.5267175572519, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320816, Length=571, Percent_Identity=25.3940455341506, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=537, Percent_Identity=24.7672253258845, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323073, Length=530, Percent_Identity=25.0943396226415, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6320123, Length=491, Percent_Identity=24.0325865580448, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922626, Length=487, Percent_Identity=26.0780287474333, Blast_Score=159, Evalue=6e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61859; Mature: 61727

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGSTDKVNGAKALIKCLEKEGVDTLFGYPGGQIIPFYNELYDSDLRHILVRHEQAAAH
CCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHH
AADGYARATGKTGVCVSTSGPGATNLVTGIATAYMDSVPIVALTGQVPRSLIGNDAFQEA
HCCCHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCC
DITGITMPITKHNYLVQDPQEIPRIVKEAFHIASTGRPGPVLIDLPKDVQNIEIDLHYPE
CCCEEEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCC
RVELRGYKPTYKGNTQQIKRAAEEIANSCRPIIYAGGGVISSNASAELVEFAETIKAPVT
EEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCHHHHHHHHHHHCCCHH
TTLMGISSIPTEHPLYVGMLGMHGCKYANYAIQESDLIIAVGARFDDRVTGKLESFAPNA
HHHHHHHCCCCCCCEEEEEHHCCCCCCCCCEEECCCEEEEECCCCCCCHHCCHHHCCCCC
RVIHIDVDPAEISKNVKVHIPIVGDAKQVLKSLIRYVQCCRSAEWIEKVNLWKKEYPLNY
EEEEEECCHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCH
RECRDTIMPQFVIEQISEVCRDAIIVTEVGQHQMWAAQFFKYSKPRTFLTSGGLGTMGYG
HHHHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCCCCC
FPAAMGAKVGRPDKTVINIAGDGSFQMNSQELATVVQNDIPVVSVILNNGYLGMVRQWQE
CHHHHCCCCCCCCCEEEEEECCCCEEECHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
LFYDRRYSYTFIKGSVDFVKLAEAYGALGLRAERPSEVRPAIEEAVNSGRPTVVEVIVEC
HHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEC
EANVYPMVPAGAAINEIIDLEEHR
CCCEEECCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TTGSTDKVNGAKALIKCLEKEGVDTLFGYPGGQIIPFYNELYDSDLRHILVRHEQAAAH
CCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHH
AADGYARATGKTGVCVSTSGPGATNLVTGIATAYMDSVPIVALTGQVPRSLIGNDAFQEA
HCCCHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCC
DITGITMPITKHNYLVQDPQEIPRIVKEAFHIASTGRPGPVLIDLPKDVQNIEIDLHYPE
CCCEEEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCC
RVELRGYKPTYKGNTQQIKRAAEEIANSCRPIIYAGGGVISSNASAELVEFAETIKAPVT
EEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCHHHHHHHHHHHCCCHH
TTLMGISSIPTEHPLYVGMLGMHGCKYANYAIQESDLIIAVGARFDDRVTGKLESFAPNA
HHHHHHHCCCCCCCEEEEEHHCCCCCCCCCEEECCCEEEEECCCCCCCHHCCHHHCCCCC
RVIHIDVDPAEISKNVKVHIPIVGDAKQVLKSLIRYVQCCRSAEWIEKVNLWKKEYPLNY
EEEEEECCHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCH
RECRDTIMPQFVIEQISEVCRDAIIVTEVGQHQMWAAQFFKYSKPRTFLTSGGLGTMGYG
HHHHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCCCCC
FPAAMGAKVGRPDKTVINIAGDGSFQMNSQELATVVQNDIPVVSVILNNGYLGMVRQWQE
CHHHHCCCCCCCCCEEEEEECCCCEEECHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
LFYDRRYSYTFIKGSVDFVKLAEAYGALGLRAERPSEVRPAIEEAVNSGRPTVVEVIVEC
HHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEC
EANVYPMVPAGAAINEIIDLEEHR
CCCEEECCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]