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The map label for this gene is leuD1

Identifier: 21226747

GI number: 21226747

Start: 771981

End: 772490

Strand: Reverse

Name: leuD1

Synonym: MM_0645

Alternate gene names: 21226747

Gene position: 772490-771981 (Counterclockwise)

Preceding gene: 21226748

Following gene: 21226746

Centisome position: 18.86

GC content: 45.49

Gene sequence:

>510_bases
GTGATGGAAAACCCTATTAAAGGCCGGGTCTGGAAATTCGGAAACGATATAGATACTGATGTAATTATTCCGGGAAAATA
CCTGAGGACAAAGGATATGCAGGTCTTTGCGGCTCATGCAATGGAAGGTATTGACCCCGGGTTCTCAAAGAAAGCAAAGC
CCGGAGATATCATTGTCGCAGGGGACAATTTCGGGTGCGGGTCATCAAGAGAACAGGCTCCTCTGGCTTTGAAGCACGCC
GGAATAGCCTGTATCGTCGCAAAATCTTTTGCAAGGATCTTTTTCAGGAATGCAATTAATATTGGTCTGCCTCTAATGGA
AGCCGACATTGAATGTGAGGAAGGGGACCAAATTGAAGTTGACCTGCTTAAAGGTGAAGTTAAAGTTTCCGGAAAAGGTG
TTTTCAGGGGAAACAAACTTCCTGACTTCCTTCTCGATATGCTCACTGACGGCGGGCTTGTTGCCCATAGAAAAAAAGTC
CGGGATCAGGAGAAAGAAGAATCAGCTTAA

Upstream 100 bases:

>100_bases
ACATCTGCTTTCTTCCAGTCTTTAATTTCCTTTATTATTTTTTCCTTTTTATATCGCTATCTAGATATACTATTAAAAAA
ACCTATTTTAAAGGTGACGT

Downstream 100 bases:

>100_bases
TGGACAATTAAATAAATCAGTTTAACGGACGATTATAGCAAAATTAATGAAATATGCTTTGTTGCCTGGTCTGTAGATTG
ATCGGGCCAGCAGGGTAACA

Product: 3-isopropylmalate dehydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MMENPIKGRVWKFGNDIDTDVIIPGKYLRTKDMQVFAAHAMEGIDPGFSKKAKPGDIIVAGDNFGCGSSREQAPLALKHA
GIACIVAKSFARIFFRNAINIGLPLMEADIECEEGDQIEVDLLKGEVKVSGKGVFRGNKLPDFLLDMLTDGGLVAHRKKV
RDQEKEESA

Sequences:

>Translated_169_residues
MMENPIKGRVWKFGNDIDTDVIIPGKYLRTKDMQVFAAHAMEGIDPGFSKKAKPGDIIVAGDNFGCGSSREQAPLALKHA
GIACIVAKSFARIFFRNAINIGLPLMEADIECEEGDQIEVDLLKGEVKVSGKGVFRGNKLPDFLLDMLTDGGLVAHRKKV
RDQEKEESA
>Mature_169_residues
MMENPIKGRVWKFGNDIDTDVIIPGKYLRTKDMQVFAAHAMEGIDPGFSKKAKPGDIIVAGDNFGCGSSREQAPLALKHA
GIACIVAKSFARIFFRNAINIGLPLMEADIECEEGDQIEVDLLKGEVKVSGKGVFRGNKLPDFLLDMLTDGGLVAHRKKV
RDQEKEESA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=144, Percent_Identity=34.7222222222222, Blast_Score=72, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=96, Percent_Identity=47.9166666666667, Blast_Score=83, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=103, Percent_Identity=38.8349514563107, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD1_METMA (Q8PZ49)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_632669.1
- ProteinModelPortal:   Q8PZ49
- SMR:   Q8PZ49
- GeneID:   1478987
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_0645
- NMPDR:   fig|192952.1.peg.645
- HOGENOM:   HBG304838
- OMA:   LASHCMA
- ProtClustDB:   CLSK568816
- BioCyc:   MMAZ192952:MM0645-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 18490; Mature: 18490

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMENPIKGRVWKFGNDIDTDVIIPGKYLRTKDMQVFAAHAMEGIDPGFSKKAKPGDIIVA
CCCCCCCCCEEECCCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEE
GDNFGCGSSREQAPLALKHAGIACIVAKSFARIFFRNAINIGLPLMEADIECEEGDQIEV
CCCCCCCCCCCCCCEEEHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEE
DLLKGEVKVSGKGVFRGNKLPDFLLDMLTDGGLVAHRKKVRDQEKEESA
EEEECEEEECCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMENPIKGRVWKFGNDIDTDVIIPGKYLRTKDMQVFAAHAMEGIDPGFSKKAKPGDIIVA
CCCCCCCCCEEECCCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEE
GDNFGCGSSREQAPLALKHAGIACIVAKSFARIFFRNAINIGLPLMEADIECEEGDQIEV
CCCCCCCCCCCCCCEEEHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEE
DLLKGEVKVSGKGVFRGNKLPDFLLDMLTDGGLVAHRKKVRDQEKEESA
EEEECEEEECCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824