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The map label for this gene is aksF [H]

Identifier: 21226744

GI number: 21226744

Start: 769271

End: 770263

Strand: Reverse

Name: aksF [H]

Synonym: MM_0642

Alternate gene names: 21226744

Gene position: 770263-769271 (Counterclockwise)

Preceding gene: 21226745

Following gene: 21226743

Centisome position: 18.8

GC content: 47.43

Gene sequence:

>993_bases
ATGAGACTCGCGGTAATCGAAGGTGACGGTATCGGCAGGGAAGTGATTCCCGCAGCTGTTGAGGTCCTGGACGCCTTCGG
GCTTGAATTCGAAAAAGTGCCTCTGGAAATCGGCTATGCAAGGTGGGAAAGAACAGGCACTGCAATGTCAGAAGAAGATA
TGGAAATTATAAAGAGCTGCAATGCTGTGCTTTTCGGAGCAGTCACCACGGTACCTGACCCAGGTTATAAAAGTGTTCTC
CTGACTATCAGGAAAGAGCTTGACCTTTACGCCAATGTCAGGCCTGTAAAACCCCTGTCTGGCGTTGTGGGAGTTTCCGG
AAGAAGCGATTTTGATTTCATTATCGTAAGGGAAAATACTGAAGGGCTTTATTCCGGGATCGAGGAGATAGGACATGATC
TTTCCTGGACAAAAAGGGTGATAACCCGAAAGGGTTCTGAGAGGATTTCGGAATACGCCTGCGAGCTTGCAAAAAAAAGG
TCCGGCAAATTAACCATTGTCCACAAGTCCAATGTCCTGAAGTCAGACAAACTTTTCCTTGATGTCTGCAGGGAAACTGC
CAGCTCTTATGGAGTTGAGTATGGAGACATGCTGGTTGACTCCATGGCTTACAGCCTTATAATGCACCCCGAAAAGTATG
ATGTTGTGGTAACAACAAACCTTTTCGGAGATATCCTGAGTGATATGTGTGCAGCCCTTGTGGGAAGCCTTGGGCTTGTC
CCTAGCGCCAATATAGGGAAAAAGTACGCCTTCTTTGAACCCGTGCACGGGAGTGCCCCTGATATTGCGGGAAAAGGAAT
TTCAAACCCTCTTGCAGCAATTCTCTGCGTAAAAATGCTGCTCGAATGGATGGGCAAACCCGAAGCCCGACTAATTGATG
CTGCAATAGCTGACCTCCTTCAGAAAAAAATAATTACTCCTGACCTGGGAGGCAGGGCGAGCACTGCAGAAGTGGGTAAT
GCGGTTGCAAAGTATATAATGCAGAACCTTTAA

Upstream 100 bases:

>100_bases
TAAAAATCCTTGCCGAACATATGGACTGGATCTGAAAATTACCTTAGAATCTTATAAAGTTACGCCAGATTCATATACCT
CAAGCCATAGATGCAGTACC

Downstream 100 bases:

>100_bases
CTCTGAATAATGCAGAAACTTAATTCAGAATGATGCAGAGCCTTAATTCAGGATAATGCAGAACTTTAACTCAGAATCCG
AAGCCTCGAAAATTTACAGG

Product: isocitrate dehydrogenase [NADP]

Products: NA

Alternate protein names: HICDH; Isohomocitrate dehydrogenase; IHDH; NAD-dependent threo-isohomocitrate dehydrogenase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MRLAVIEGDGIGREVIPAAVEVLDAFGLEFEKVPLEIGYARWERTGTAMSEEDMEIIKSCNAVLFGAVTTVPDPGYKSVL
LTIRKELDLYANVRPVKPLSGVVGVSGRSDFDFIIVRENTEGLYSGIEEIGHDLSWTKRVITRKGSERISEYACELAKKR
SGKLTIVHKSNVLKSDKLFLDVCRETASSYGVEYGDMLVDSMAYSLIMHPEKYDVVVTTNLFGDILSDMCAALVGSLGLV
PSANIGKKYAFFEPVHGSAPDIAGKGISNPLAAILCVKMLLEWMGKPEARLIDAAIADLLQKKIITPDLGGRASTAEVGN
AVAKYIMQNL

Sequences:

>Translated_330_residues
MRLAVIEGDGIGREVIPAAVEVLDAFGLEFEKVPLEIGYARWERTGTAMSEEDMEIIKSCNAVLFGAVTTVPDPGYKSVL
LTIRKELDLYANVRPVKPLSGVVGVSGRSDFDFIIVRENTEGLYSGIEEIGHDLSWTKRVITRKGSERISEYACELAKKR
SGKLTIVHKSNVLKSDKLFLDVCRETASSYGVEYGDMLVDSMAYSLIMHPEKYDVVVTTNLFGDILSDMCAALVGSLGLV
PSANIGKKYAFFEPVHGSAPDIAGKGISNPLAAILCVKMLLEWMGKPEARLIDAAIADLLQKKIITPDLGGRASTAEVGN
AVAKYIMQNL
>Mature_330_residues
MRLAVIEGDGIGREVIPAAVEVLDAFGLEFEKVPLEIGYARWERTGTAMSEEDMEIIKSCNAVLFGAVTTVPDPGYKSVL
LTIRKELDLYANVRPVKPLSGVVGVSGRSDFDFIIVRENTEGLYSGIEEIGHDLSWTKRVITRKGSERISEYACELAKKR
SGKLTIVHKSNVLKSDKLFLDVCRETASSYGVEYGDMLVDSMAYSLIMHPEKYDVVVTTNLFGDILSDMCAALVGSLGLV
PSANIGKKYAFFEPVHGSAPDIAGKGISNPLAAILCVKMLLEWMGKPEARLIDAAIADLLQKKIITPDLGGRASTAEVGN
AVAKYIMQNL

Specific function: Catalyzes the NAD-dependent oxidation and decarboxylation of (2R,3S)-homoisocitrate ((1R,2S)-1- hydroxybutane-1,2,4-tricarboxylate), (2R,3S)-homo(2)-isocitrate and (2R,3S)-homo(3)-isocitrate, into 2-oxoadipate, 2-oxopimelate, and 2-oxosuberate, respective

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=341, Percent_Identity=38.1231671554252, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI4758582, Length=340, Percent_Identity=36.4705882352941, Blast_Score=177, Evalue=8e-45,
Organism=Homo sapiens, GI28178838, Length=296, Percent_Identity=38.1756756756757, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI28178816, Length=336, Percent_Identity=32.7380952380952, Blast_Score=154, Evalue=7e-38,
Organism=Homo sapiens, GI28178821, Length=340, Percent_Identity=32.0588235294118, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI28178819, Length=195, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1788101, Length=358, Percent_Identity=39.6648044692737, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI87081683, Length=361, Percent_Identity=36.5650969529086, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1787381, Length=364, Percent_Identity=29.9450549450549, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71986051, Length=321, Percent_Identity=37.3831775700935, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17550882, Length=336, Percent_Identity=33.6309523809524, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI25144293, Length=343, Percent_Identity=34.1107871720117, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17505779, Length=339, Percent_Identity=34.2182890855457, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6322097, Length=344, Percent_Identity=44.7674418604651, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324709, Length=369, Percent_Identity=34.9593495934959, Blast_Score=176, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6319830, Length=360, Percent_Identity=36.3888888888889, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6324291, Length=340, Percent_Identity=34.4117647058824, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24643270, Length=327, Percent_Identity=38.5321100917431, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24643268, Length=327, Percent_Identity=38.5321100917431, Blast_Score=218, Evalue=5e-57,
Organism=Drosophila melanogaster, GI24661184, Length=337, Percent_Identity=37.3887240356083, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI161078635, Length=332, Percent_Identity=31.9277108433735, Blast_Score=147, Evalue=8e-36,
Organism=Drosophila melanogaster, GI161078633, Length=332, Percent_Identity=31.9277108433735, Blast_Score=147, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24650122, Length=332, Percent_Identity=31.9277108433735, Blast_Score=147, Evalue=9e-36,
Organism=Drosophila melanogaster, GI161078637, Length=332, Percent_Identity=31.9277108433735, Blast_Score=147, Evalue=9e-36,
Organism=Drosophila melanogaster, GI161078639, Length=330, Percent_Identity=32.1212121212121, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI281362242, Length=332, Percent_Identity=32.2289156626506, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24648872, Length=332, Percent_Identity=32.2289156626506, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI20130355, Length=333, Percent_Identity=28.2282282282282, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.87 [H]

Molecular weight: Translated: 35867; Mature: 35867

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAVIEGDGIGREVIPAAVEVLDAFGLEFEKVPLEIGYARWERTGTAMSEEDMEIIKSC
CEEEEEECCCCCHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHC
NAVLFGAVTTVPDPGYKSVLLTIRKELDLYANVRPVKPLSGVVGVSGRSDFDFIIVRENT
CHHEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCEEEEECCC
EGLYSGIEEIGHDLSWTKRVITRKGSERISEYACELAKKRSGKLTIVHKSNVLKSDKLFL
CHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
DVCRETASSYGVEYGDMLVDSMAYSLIMHPEKYDVVVTTNLFGDILSDMCAALVGSLGLV
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCC
PSANIGKKYAFFEPVHGSAPDIAGKGISNPLAAILCVKMLLEWMGKPEARLIDAAIADLL
CCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
QKKIITPDLGGRASTAEVGNAVAKYIMQNL
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLAVIEGDGIGREVIPAAVEVLDAFGLEFEKVPLEIGYARWERTGTAMSEEDMEIIKSC
CEEEEEECCCCCHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHC
NAVLFGAVTTVPDPGYKSVLLTIRKELDLYANVRPVKPLSGVVGVSGRSDFDFIIVRENT
CHHEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCEEEEECCC
EGLYSGIEEIGHDLSWTKRVITRKGSERISEYACELAKKRSGKLTIVHKSNVLKSDKLFL
CHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
DVCRETASSYGVEYGDMLVDSMAYSLIMHPEKYDVVVTTNLFGDILSDMCAALVGSLGLV
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCC
PSANIGKKYAFFEPVHGSAPDIAGKGISNPLAAILCVKMLLEWMGKPEARLIDAAIADLL
CCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
QKKIITPDLGGRASTAEVGNAVAKYIMQNL
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 10940051 [H]