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The map label for this gene is lacA [C]

Identifier: 21226712

GI number: 21226712

Start: 740969

End: 741532

Strand: Reverse

Name: lacA [C]

Synonym: MM_0610

Alternate gene names: 21226712

Gene position: 741532-740969 (Counterclockwise)

Preceding gene: 21226713

Following gene: 21226709

Centisome position: 18.1

GC content: 45.57

Gene sequence:

>564_bases
ATGGGGGGCATTACCATAACTGCCGTAATATCAAACAGAGATAAAGTAACTTTTGGTGAAGTGGCTGATCATTACCACAG
CAATAAACTCATTTTTGGGCTGAAGTACTTTAAGAACTGGGTTCTGGAACGCGTTGCTGCGTGGTTCCCGATTCCTTCGT
GGAGAGCTAAATTTCATAAAATGAGAGGCGTGAATCTCGGGAAAAACGTGTATGTAGGCTACGATGTGGTTTTTGACAGG
CTTCACCCTGAAATGATCACAGTGGGAGATTACTCTGAAATAGGAGACCGCTGCATTCTCTCTGCCCATTCCAGAGGCAG
TCTGACCACAAGGCAGGCATATCCGAGGACCATAGAGCCCATAAAAATAGGGTGTGGAGTTTCTGTAAATCCAGGCTGCA
TAATTACTCAGGGGGTCGAGATCGGGGATAACTCAATAATCGGTATAGGTTCCGTGGTTACTCGTGACATTTCTCCAAAC
AGCCTTGCCCTTGGTTATCCGGCTCGAGTTATCAAAAAACTTGACGGTGTGGGTGAGCTCCAGACCAGTTTTAATGATAC
TTAA

Upstream 100 bases:

>100_bases
GAATCCGGTAGGAAATGTGCCGGTCCAGGGCTGACAATGGGAAATATATTTATCAGTAGAAAAGTAATGAATATAAGTAC
CAGGCGTTTTTGACCGCAAA

Downstream 100 bases:

>100_bases
TTTATTTTTTATTACAACGGGAGCGCAGAGGGTCACGAATACCCCTTCCTTTAGGTAGGGGATGAAGTGAACCCTCGCCT
CTTCGTTTTTCTCTTTAAAT

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MGGITITAVISNRDKVTFGEVADHYHSNKLIFGLKYFKNWVLERVAAWFPIPSWRAKFHKMRGVNLGKNVYVGYDVVFDR
LHPEMITVGDYSEIGDRCILSAHSRGSLTTRQAYPRTIEPIKIGCGVSVNPGCIITQGVEIGDNSIIGIGSVVTRDISPN
SLALGYPARVIKKLDGVGELQTSFNDT

Sequences:

>Translated_187_residues
MGGITITAVISNRDKVTFGEVADHYHSNKLIFGLKYFKNWVLERVAAWFPIPSWRAKFHKMRGVNLGKNVYVGYDVVFDR
LHPEMITVGDYSEIGDRCILSAHSRGSLTTRQAYPRTIEPIKIGCGVSVNPGCIITQGVEIGDNSIIGIGSVVTRDISPN
SLALGYPARVIKKLDGVGELQTSFNDT
>Mature_186_residues
GGITITAVISNRDKVTFGEVADHYHSNKLIFGLKYFKNWVLERVAAWFPIPSWRAKFHKMRGVNLGKNVYVGYDVVFDRL
HPEMITVGDYSEIGDRCILSAHSRGSLTTRQAYPRTIEPIKIGCGVSVNPGCIITQGVEIGDNSIIGIGSVVTRDISPNS
LALGYPARVIKKLDGVGELQTSFNDT

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=137, Percent_Identity=32.1167883211679, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 20585; Mature: 20454

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGITITAVISNRDKVTFGEVADHYHSNKLIFGLKYFKNWVLERVAAWFPIPSWRAKFHK
CCCEEEEEEECCCCCEEHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHH
MRGVNLGKNVYVGYDVVFDRLHPEMITVGDYSEIGDRCILSAHSRGSLTTRQAYPRTIEP
HCCCCCCCEEEEEHHHHHHHCCCCEEEECCHHHHCCEEEEECCCCCCEEECCCCCCCCCC
IKIGCGVSVNPGCIITQGVEIGDNSIIGIGSVVTRDISPNSLALGYPARVIKKLDGVGEL
EEECCCCCCCCCEEEECCEEECCCCEEEECHHEECCCCCCCEEECCHHHHHHHHCCCCHH
QTSFNDT
HHCCCCC
>Mature Secondary Structure 
GGITITAVISNRDKVTFGEVADHYHSNKLIFGLKYFKNWVLERVAAWFPIPSWRAKFHK
CCEEEEEEECCCCCEEHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHH
MRGVNLGKNVYVGYDVVFDRLHPEMITVGDYSEIGDRCILSAHSRGSLTTRQAYPRTIEP
HCCCCCCCEEEEEHHHHHHHCCCCEEEECCHHHHCCEEEEECCCCCCEEECCCCCCCCCC
IKIGCGVSVNPGCIITQGVEIGDNSIIGIGSVVTRDISPNSLALGYPARVIKKLDGVGEL
EEECCCCCCCCCEEEECCEEECCCCEEEECHHEECCCCCCCEEECCHHHHHHHHCCCCHH
QTSFNDT
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA