| Definition | Methanosarcina mazei Go1 chromosome, complete genome. |
|---|---|
| Accession | NC_003901 |
| Length | 4,096,345 |
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The map label for this gene is nifH2 [H]
Identifier: 21226616
GI number: 21226616
Start: 639362
End: 640159
Strand: Reverse
Name: nifH2 [H]
Synonym: MM_0514
Alternate gene names: 21226616
Gene position: 640159-639362 (Counterclockwise)
Preceding gene: 21226617
Following gene: 21226615
Centisome position: 15.63
GC content: 48.87
Gene sequence:
>798_bases TTGAAAAAACAAAAGATTATTGCAATTTACGGAAAAGGCGGCATAGGCAAGTCGAGCACAGCTTCAAATGTTGCAGCCGC CTGTGCGGAAGAAGGCAAGAAAGTTATGATCATAGGCTGTGACCCCAAAAGCGATTCTTCGATAACCCTTCTCAGAGGGC GGAGAATTCCGACAATTCTTGACCTCCTCCGCGAAGGCGTGGATGTTAAGGAAAAAGACATTGTCTTTGAAGGGTATGCA GGCGTCAAATGCGTGGAAGCCGGAGGCCCGGAGCCTGGAATAGGCTGTGCAGGACGCGGAATTATAGTCGCAATCCAGAA ATTAAAGAGCGTCACCGGAGACCTTCTGAAAGAACAGGACCTCATAATCTACGATGTACCCGGGGATATCGTCTGCGGCG GGTTTGTTGCCCCTGTCAGGAAAGGCTTTGTAAACGAGGCTTATGTCCTGACCTCGGGAGAGTACATGCCCCTTTATGCA GCAAACAATATCTGTAAAGGGCTTTCCAAAATCGGGATGCCTCTCAGCGGAGTTATCTGCAACTCAAGAAACGCAAGCAG GGAAGAGGAAATTGTCAGAAAGTTTTCCGAAGAAATCGGCAGCCAGTTAATGGCTTTCATTCCAAAGAGGCAGGCAGTAC AGGACTGGGAGAGGGAAGGCTATTCCGTTATGGAGAAAGCTCCTGACTCCGATATTGCCGAAGTCTACCGCCAGCTTGGA AAAGCAATCCTGGAAAATGAAAAAAAGGTTATGGCAGACCACCTGGGCGATGAGCGGCTGAGGGAAATGACAAAGTAA
Upstream 100 bases:
>100_bases AAGGAAGCCTGAAATGGGAAAGAAAGACCGGATAAAAAACCAGAGTCTATGATAAATCAGGATAAAGCCGGAAAATATTC AATAGCGGGAGGAAGATCCT
Downstream 100 bases:
>100_bases ACCGGAAGTTCTGGAACGCGAACTGGAAATAATTGAAAATAAGCGAAACATGAAAAAATAAATTCTTAAAAGCAGCATAA CTGCAAAACTATCCGTAAAA
Product: nitrogenase iron protein 2
Products: NA
Alternate protein names: Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase [H]
Number of amino acids: Translated: 265; Mature: 265
Protein sequence:
>265_residues MKKQKIIAIYGKGGIGKSSTASNVAAACAEEGKKVMIIGCDPKSDSSITLLRGRRIPTILDLLREGVDVKEKDIVFEGYA GVKCVEAGGPEPGIGCAGRGIIVAIQKLKSVTGDLLKEQDLIIYDVPGDIVCGGFVAPVRKGFVNEAYVLTSGEYMPLYA ANNICKGLSKIGMPLSGVICNSRNASREEEIVRKFSEEIGSQLMAFIPKRQAVQDWEREGYSVMEKAPDSDIAEVYRQLG KAILENEKKVMADHLGDERLREMTK
Sequences:
>Translated_265_residues MKKQKIIAIYGKGGIGKSSTASNVAAACAEEGKKVMIIGCDPKSDSSITLLRGRRIPTILDLLREGVDVKEKDIVFEGYA GVKCVEAGGPEPGIGCAGRGIIVAIQKLKSVTGDLLKEQDLIIYDVPGDIVCGGFVAPVRKGFVNEAYVLTSGEYMPLYA ANNICKGLSKIGMPLSGVICNSRNASREEEIVRKFSEEIGSQLMAFIPKRQAVQDWEREGYSVMEKAPDSDIAEVYRQLG KAILENEKKVMADHLGDERLREMTK >Mature_265_residues MKKQKIIAIYGKGGIGKSSTASNVAAACAEEGKKVMIIGCDPKSDSSITLLRGRRIPTILDLLREGVDVKEKDIVFEGYA GVKCVEAGGPEPGIGCAGRGIIVAIQKLKSVTGDLLKEQDLIIYDVPGDIVCGGFVAPVRKGFVNEAYVLTSGEYMPLYA ANNICKGLSKIGMPLSGVICNSRNASREEEIVRKFSEEIGSQLMAFIPKRQAVQDWEREGYSVMEKAPDSDIAEVYRQLG KAILENEKKVMADHLGDERLREMTK
Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]
COG id: COG1348
COG function: function code P; Nitrogenase subunit NifH (ATPase)
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the NifH/BchL/ChlL family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000392 - InterPro: IPR005977 [H]
Pfam domain/function: PF00142 Fer4_NifH [H]
EC number: =1.18.6.1 [H]
Molecular weight: Translated: 28760; Mature: 28760
Theoretical pI: Translated: 7.68; Mature: 7.68
Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00746 NIFH_FRXC_1 ; PS51026 NIFH_FRXC_3
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.6 %Cys (Translated Protein) 3.0 %Met (Translated Protein) 5.7 %Cys+Met (Translated Protein) 2.6 %Cys (Mature Protein) 3.0 %Met (Mature Protein) 5.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKKQKIIAIYGKGGIGKSSTASNVAAACAEEGKKVMIIGCDPKSDSSITLLRGRRIPTIL CCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCCHHHH DLLREGVDVKEKDIVFEGYAGVKCVEAGGPEPGIGCAGRGIIVAIQKLKSVTGDLLKEQD HHHHCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC LIIYDVPGDIVCGGFVAPVRKGFVNEAYVLTSGEYMPLYAANNICKGLSKIGMPLSGVIC EEEEECCCCEEECCHHHHHHHCCCCCEEEEECCCEEEEHHHHHHHHHHHHHCCCCEEEEE NSRNASREEEIVRKFSEEIGSQLMAFIPKRQAVQDWEREGYSVMEKAPDSDIAEVYRQLG CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHH KAILENEKKVMADHLGDERLREMTK HHHHHCHHHHHHHHCCHHHHHHHCC >Mature Secondary Structure MKKQKIIAIYGKGGIGKSSTASNVAAACAEEGKKVMIIGCDPKSDSSITLLRGRRIPTIL CCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCCHHHH DLLREGVDVKEKDIVFEGYAGVKCVEAGGPEPGIGCAGRGIIVAIQKLKSVTGDLLKEQD HHHHCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC LIIYDVPGDIVCGGFVAPVRKGFVNEAYVLTSGEYMPLYAANNICKGLSKIGMPLSGVIC EEEEECCCCEEECCHHHHHHHCCCCCEEEEECCCEEEEHHHHHHHHHHHHHCCCCEEEEE NSRNASREEEIVRKFSEEIGSQLMAFIPKRQAVQDWEREGYSVMEKAPDSDIAEVYRQLG CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHH KAILENEKKVMADHLGDERLREMTK HHHHHCHHHHHHHHCCHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: NA