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The map label for this gene is mtaC [H]

Identifier: 21226276

GI number: 21226276

Start: 238003

End: 238785

Strand: Direct

Name: mtaC [H]

Synonym: MM_0174

Alternate gene names: 21226276

Gene position: 238003-238785 (Clockwise)

Preceding gene: 21226275

Following gene: 21226277

Centisome position: 5.81

GC content: 43.3

Gene sequence:

>783_bases
ATGGAGGTTAAATATTTGATAGAAATAGATCCCAGTATGATTCTGGTGCGTTATAATGTAAAAATGGAAAAGCAAATGAC
ACCAGAAGACGCTGCAGAAGAACTTTACCCAAAAGATGAATTAATTTATCCGATAGCAAAGGCCATTTTTGAAGGCGAAG
AAGATGACGTTGTAGAAGGACTGGAAAAGGCCATTAGCTCTGGAAAGGACCCAATCTCCCTAATAGATGACGCATTGATG
GTTGGTATGGGGGTAGTCACCAGACTTTACGATGAAGGAATTATTTTCCTGCCAAACGTTATGATGTCTGCTGACGCTAT
GCTGGAAGGTATCGACCATGTTAAGCAGAAGGCAGGAAAGGCACCCACAGTAAAAGGAAAAGTAGTATGCCATGTTGCAG
AAGGTGACGTCCATGATATCGGAAAGAATATTGTGGCTGCACTACTGAGAGCAGCTGGCTATGACGTTGTAGACCTTGGA
AGAGATGTTCCTGTAGATGAGGTCATCGCAACTGTTGAAAAAGAAAATCCCATGATGCTAACAGGTACTGCTCTGATGAC
CACGACCATGTATGCATTCAAGGAAGTTAATGATAAGCTTATGGAGAAAGGCTACAGGATCCCATTTGCATGTGGTGGAG
GTGCTGTGAATCAGGATTTCGTGTCCCAGTATGAGCTTGGGGTTTATGGTGAAGAAGCAGCCGATGCCGCTAAAATAGCC
GATTTCATCAAGGCAAATGGGGACAACGTTGTGAAACTAAGGGAGAAATTCCATAGACACTGA

Upstream 100 bases:

>100_bases
GAATTAGAGGGTATCTCGGTATTTTATAGGTCTTACCAGGAACTTTTTTAGCCCGACCTTTACCCCTACTTAAACCAGGG
ATTGCTCCTCAAGAGCTTAA

Downstream 100 bases:

>100_bases
GGAGGTGGAGTAGATGGCAGTAAACAGATGTACTAAAATGTCTTATGCAAGCGCAGATGAAATGATCTTTGGAAAAGCCG
TCAAGCCCGTCAAAGCAGGG

Product: methanol corrinoid protein

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MEVKYLIEIDPSMILVRYNVKMEKQMTPEDAAEELYPKDELIYPIAKAIFEGEEDDVVEGLEKAISSGKDPISLIDDALM
VGMGVVTRLYDEGIIFLPNVMMSADAMLEGIDHVKQKAGKAPTVKGKVVCHVAEGDVHDIGKNIVAALLRAAGYDVVDLG
RDVPVDEVIATVEKENPMMLTGTALMTTTMYAFKEVNDKLMEKGYRIPFACGGGAVNQDFVSQYELGVYGEEAADAAKIA
DFIKANGDNVVKLREKFHRH

Sequences:

>Translated_260_residues
MEVKYLIEIDPSMILVRYNVKMEKQMTPEDAAEELYPKDELIYPIAKAIFEGEEDDVVEGLEKAISSGKDPISLIDDALM
VGMGVVTRLYDEGIIFLPNVMMSADAMLEGIDHVKQKAGKAPTVKGKVVCHVAEGDVHDIGKNIVAALLRAAGYDVVDLG
RDVPVDEVIATVEKENPMMLTGTALMTTTMYAFKEVNDKLMEKGYRIPFACGGGAVNQDFVSQYELGVYGEEAADAAKIA
DFIKANGDNVVKLREKFHRH
>Mature_260_residues
MEVKYLIEIDPSMILVRYNVKMEKQMTPEDAAEELYPKDELIYPIAKAIFEGEEDDVVEGLEKAISSGKDPISLIDDALM
VGMGVVTRLYDEGIIFLPNVMMSADAMLEGIDHVKQKAGKAPTVKGKVVCHVAEGDVHDIGKNIVAALLRAAGYDVVDLG
RDVPVDEVIATVEKENPMMLTGTALMTTTMYAFKEVNDKLMEKGYRIPFACGGGAVNQDFVSQYELGVYGEEAADAAKIA
DFIKANGDNVVKLREKFHRH

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=188, Percent_Identity=28.7234042553192, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1790450, Length=181, Percent_Identity=33.1491712707182, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17535405, Length=163, Percent_Identity=31.2883435582822, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 28549; Mature: 28549

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVKYLIEIDPSMILVRYNVKMEKQMTPEDAAEELYPKDELIYPIAKAIFEGEEDDVVEG
CCEEEEEEECCCEEEEEECCEECCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
LEKAISSGKDPISLIDDALMVGMGVVTRLYDEGIIFLPNVMMSADAMLEGIDHVKQKAGK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHCC
APTVKGKVVCHVAEGDVHDIGKNIVAALLRAAGYDVVDLGRDVPVDEVIATVEKENPMML
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHCCCCEEE
TGTALMTTTMYAFKEVNDKLMEKGYRIPFACGGGAVNQDFVSQYELGVYGEEAADAAKIA
EHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHH
DFIKANGDNVVKLREKFHRH
HHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MEVKYLIEIDPSMILVRYNVKMEKQMTPEDAAEELYPKDELIYPIAKAIFEGEEDDVVEG
CCEEEEEEECCCEEEEEECCEECCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
LEKAISSGKDPISLIDDALMVGMGVVTRLYDEGIIFLPNVMMSADAMLEGIDHVKQKAGK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHCC
APTVKGKVVCHVAEGDVHDIGKNIVAALLRAAGYDVVDLGRDVPVDEVIATVEKENPMML
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHCCCCEEE
TGTALMTTTMYAFKEVNDKLMEKGYRIPFACGGGAVNQDFVSQYELGVYGEEAADAAKIA
EHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHH
DFIKANGDNVVKLREKFHRH
HHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]