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The map label for this gene is azoB [H]

Identifier: 21226252

GI number: 21226252

Start: 185898

End: 186767

Strand: Direct

Name: azoB [H]

Synonym: MM_0150

Alternate gene names: 21226252

Gene position: 185898-186767 (Clockwise)

Preceding gene: 21226251

Following gene: 21226255

Centisome position: 4.54

GC content: 44.14

Gene sequence:

>870_bases
ATGATATTGTTAATTGGTGCAACAGGAAATATAGGCCTGCCAATAGTTAAAAGTCTCCAAAAGAAAGGTGCAGAGGTTCG
TCTACTTGTACACAATGAGAAATCAAGTGCAAAAATCCAAGCATTGGGTCAAATGGAAATTTTTATAGGAGATTTTCGTA
ACGACTCTGATCTGAGGGAATCCATGAAAGGTTGCAGCTCTGTATTCCATGTCGTCCCTCCTTTCACAAAGGATGAAGCT
GAGATTGGTTATCGTGTGATTGAGAATGCACGTCATGCTGGCGTTGAACATATCGTCTTCAATTCTGTTCTTCATCCACA
GCTGCGCAAGATGGAACATCATGCTCAGAAACTCTTGGTGGAAGAGGCAGTGATCGAATCCGGCTTGGCTTTCAACATCA
TTCAGCCTGCAATGTTGATGCAGAATCTTCTGATGGTATGGAGAACAATAAGAGAAAATGGTATATATCCGGTCATTTCC
TCTCCAGATAAAAAAATGTCTCTTATCGATTCTGAGGATATCGGAGAAGCAGTTGCCAATATCTTGACAGATCCTTCATT
GCGTAATGCGACCTTTGAGCTGGCAGGATCTGATCTGCTTACATACAAAGAGATGGCTTTGATTATCAGTGAGGAGCTGA
ATCAGCCGGTAAAAGTCGTGCCGGTGGATGACAAAGGCAGGGAGGAGCTAGCACGGAAACAGGGCATGTCCTCCTATGCT
ATCCATGCATTCCTTAAAATGGCAAAGCACTACGACGAACATGGATTTTCCGGTAGCAACCCGCTGGTGCTCACGTCAAT
CTTGAAGCGGCCCCCGAACAGTTATCGTGATTTCATCAGGCGCTTAATTGCGGAAGATGGCGGTATTTAA

Upstream 100 bases:

>100_bases
CTTAATCAAATGGATTAAAGTTGTAACAGCAATTATTTTTTCGATTGAGGGCATTGTTTATATGAAATAAAAGATCCAGA
TACGAATTCGGAGTGGAAAT

Downstream 100 bases:

>100_bases
AAACCTGCCCGTCTATCCGTTAATTTAATTCCATTCAGTTGCTCAGTGGATCAAGGGAGATCATCCCGCTCACCTTACCT
GTCTGCTGGATGAGGGAAGC

Product: putative nucleoside-diphosphate-sugar epimerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MILLIGATGNIGLPIVKSLQKKGAEVRLLVHNEKSSAKIQALGQMEIFIGDFRNDSDLRESMKGCSSVFHVVPPFTKDEA
EIGYRVIENARHAGVEHIVFNSVLHPQLRKMEHHAQKLLVEEAVIESGLAFNIIQPAMLMQNLLMVWRTIRENGIYPVIS
SPDKKMSLIDSEDIGEAVANILTDPSLRNATFELAGSDLLTYKEMALIISEELNQPVKVVPVDDKGREELARKQGMSSYA
IHAFLKMAKHYDEHGFSGSNPLVLTSILKRPPNSYRDFIRRLIAEDGGI

Sequences:

>Translated_289_residues
MILLIGATGNIGLPIVKSLQKKGAEVRLLVHNEKSSAKIQALGQMEIFIGDFRNDSDLRESMKGCSSVFHVVPPFTKDEA
EIGYRVIENARHAGVEHIVFNSVLHPQLRKMEHHAQKLLVEEAVIESGLAFNIIQPAMLMQNLLMVWRTIRENGIYPVIS
SPDKKMSLIDSEDIGEAVANILTDPSLRNATFELAGSDLLTYKEMALIISEELNQPVKVVPVDDKGREELARKQGMSSYA
IHAFLKMAKHYDEHGFSGSNPLVLTSILKRPPNSYRDFIRRLIAEDGGI
>Mature_289_residues
MILLIGATGNIGLPIVKSLQKKGAEVRLLVHNEKSSAKIQALGQMEIFIGDFRNDSDLRESMKGCSSVFHVVPPFTKDEA
EIGYRVIENARHAGVEHIVFNSVLHPQLRKMEHHAQKLLVEEAVIESGLAFNIIQPAMLMQNLLMVWRTIRENGIYPVIS
SPDKKMSLIDSEDIGEAVANILTDPSLRNATFELAGSDLLTYKEMALIISEELNQPVKVVPVDDKGREELARKQGMSSYA
IHAFLKMAKHYDEHGFSGSNPLVLTSILKRPPNSYRDFIRRLIAEDGGI

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790656, Length=221, Percent_Identity=23.0769230769231, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 32223; Mature: 32223

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLIGATGNIGLPIVKSLQKKGAEVRLLVHNEKSSAKIQALGQMEIFIGDFRNDSDLRE
CEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCEEEEEECCCCCHHHHH
SMKGCSSVFHVVPPFTKDEAEIGYRVIENARHAGVEHIVFNSVLHPQLRKMEHHAQKLLV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EEAVIESGLAFNIIQPAMLMQNLLMVWRTIRENGIYPVISSPDKKMSLIDSEDIGEAVAN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHCCCHHHHHHHHH
ILTDPSLRNATFELAGSDLLTYKEMALIISEELNQPVKVVPVDDKGREELARKQGMSSYA
HHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCHHHH
IHAFLKMAKHYDEHGFSGSNPLVLTSILKRPPNSYRDFIRRLIAEDGGI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILLIGATGNIGLPIVKSLQKKGAEVRLLVHNEKSSAKIQALGQMEIFIGDFRNDSDLRE
CEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCEEEEEECCCCCHHHHH
SMKGCSSVFHVVPPFTKDEAEIGYRVIENARHAGVEHIVFNSVLHPQLRKMEHHAQKLLV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EEAVIESGLAFNIIQPAMLMQNLLMVWRTIRENGIYPVISSPDKKMSLIDSEDIGEAVAN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHCCCHHHHHHHHH
ILTDPSLRNATFELAGSDLLTYKEMALIISEELNQPVKVVPVDDKGREELARKQGMSSYA
HHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCHHHH
IHAFLKMAKHYDEHGFSGSNPLVLTSILKRPPNSYRDFIRRLIAEDGGI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]