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The map label for this gene is lepA

Identifier: 209695945

GI number: 209695945

Start: 2709013

End: 2710806

Strand: Reverse

Name: lepA

Synonym: VSAL_I2527

Alternate gene names: 209695945

Gene position: 2710806-2709013 (Counterclockwise)

Preceding gene: 209695946

Following gene: 209695944

Centisome position: 81.52

GC content: 40.41

Gene sequence:

>1794_bases
ATGAAGCATATTCGTAATTTTTCTATTATTGCCCATATCGATCACGGTAAATCAACACTATCTGACCGCCTTATCCAAGT
TTGTGGTGGCCTGTCAGACCGTGAAATGGCAGCACAAGTTCTTGATTCAATGGATCTAGAACGTGAACGTGGTATCACTA
TTAAAGCCCAGAGTGTAACTCTAAACTACACGGCGAACGATGGTGAAACTTATCAACTAAACTTTATCGATACACCTGGA
CACGTTGATTTCTCATACGAAGTATCACGTTCACTTGCGGCTTGTGAAGGCGCATTATTAGTGGTTGATGCAGGTCAGGG
TGTAGAAGCTCAAACTCTAGCAAACTGTTATACCGCTCTTGAAATGGATTTAGAAGTCGTTCCAATCTTGAATAAGATTG
ACTTACCAGCGGCTGATCCTGATCGTGTAGCAGAAGAAATTGAAGACATCGTTGGTATTGAAGCGATGGAAGCGACACGT
TGTTCAGCAAAGACTGGGTTAGGTGTTGATGCCGTTCTTGAAACGATTGTTAAATGCATTCCAGCACCGGAAGGCAATCC
AGAAGGTCCAACCCAAGCGTTGATCATTGACTCATGGTTTGATAATTACTTAGGTGTTGTTTCTTTAGTTCGAGTTAAAC
ATGGCTCATTGAAGAAAAATGACAAAATTAAAGTAATGAGCACTGGCCAAGCATGGAATGTTGACCGCATTGGTATCTTC
ACTCCAAAACAAGTGGATACTGATGGATTAAACACAGGTGAAGTAGGCTGGGTTGTTTGTGGTATTAAAGATATTTTAGG
CGCACCAGTGGGTGATACATTAACTCACGCTAAAGGTGGCTGTGAAGAGCGCTTACCTGGTTTCCAAAAAGTAAAACCAC
AGGTATATGCTGGTTTATTCCCTATATCATCGGATGATTATGAAAACTTCCGTGATGCATTGGGTAAATTAAGCTTAAAT
GATGCGTCTCTTTTCTATGAACCAGAAAGTTCTTCAGCACTTGGTTTTGGTTTCCGTTGTGGCTTCCTTGGTATGCTACA
CATGGAAATCATTCAAGAGCGTTTAGAGCGTGAATACGATCTTGACCTTATTACAACTGCACCAACGGTAGTGTACGAAG
TTGTGTTGAATAATGGTGATTTACTGTATGTAGATAGTCCAGCAAAACTGCCAGCAGTGAACGACTTAGACGAAATACGT
GAACCAATTGCGCGTTGTAATATTTTGGTGCCAGCGGAATACTTAGGCAATGTAATTAGCCTATGTATTGAAAAGCGTGG
TACTCAAGTTGATATGATTTATCATGGCAACCAAGTGGCATTAACTTATGATATTCCAATGTCTGAAGTGGTGTTAGATT
TCTTCGACCGTTTGAAGTCAACCTCTCGTGGTTATGCATCATTAGATTACAACTTCCAACGTTATGAACTTTCTAACATG
GTTCGTGTTGACGTATTGATTAATGCTGAAAGAGTCGATGCTCTTGCTATCATTACTCACCATGATAATGCACAAGGTCG
TGGTCGTTTGCTGGTTGAGAAGATGAAAGAATTCATCCCTCGCCAAATGTTTGATATTGCAATTCAAGCAGCAATCGGCG
GGCATATTATTGCACGTTCAACGGTTAAGCAATTACGTAAAAATGTAATCGCAAAATGTTACGGTGGTGATATTAGCCGT
AAGAAAAAACTGTTGAAGAAGCAAAAAGACGGTAAGAAACGCATGAAGCAGATTGGTAATGTTGAATTACCTCAAGAAGC
TTTCCTTGCGATTCTTCATGTAGGTAAAGATTAA

Upstream 100 bases:

>100_bases
CCGCAGGATAAGATAGCGACTTGCGAGGAAATTGGGTAGAATCCCGTTACTTGATCTCACGATCCAATCCCATTAACTAA
TTGAGTTTGTCACGTCATTC

Downstream 100 bases:

>100_bases
TTTATTACTTCTGATTATTCAGAGTGAAGTAAATTAAACTTTATTACAGCTCAAAGAAAGCGATGATCAATGGTCTTCGC
TTTCTTTGTTATGCAACACT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMAAQVLDSMDLERERGITIKAQSVTLNYTANDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALEMDLEVVPILNKIDLPAADPDRVAEEIEDIVGIEAMEATR
CSAKTGLGVDAVLETIVKCIPAPEGNPEGPTQALIIDSWFDNYLGVVSLVRVKHGSLKKNDKIKVMSTGQAWNVDRIGIF
TPKQVDTDGLNTGEVGWVVCGIKDILGAPVGDTLTHAKGGCEERLPGFQKVKPQVYAGLFPISSDDYENFRDALGKLSLN
DASLFYEPESSSALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPTVVYEVVLNNGDLLYVDSPAKLPAVNDLDEIR
EPIARCNILVPAEYLGNVISLCIEKRGTQVDMIYHGNQVALTYDIPMSEVVLDFFDRLKSTSRGYASLDYNFQRYELSNM
VRVDVLINAERVDALAIITHHDNAQGRGRLLVEKMKEFIPRQMFDIAIQAAIGGHIIARSTVKQLRKNVIAKCYGGDISR
KKKLLKKQKDGKKRMKQIGNVELPQEAFLAILHVGKD

Sequences:

>Translated_597_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMAAQVLDSMDLERERGITIKAQSVTLNYTANDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALEMDLEVVPILNKIDLPAADPDRVAEEIEDIVGIEAMEATR
CSAKTGLGVDAVLETIVKCIPAPEGNPEGPTQALIIDSWFDNYLGVVSLVRVKHGSLKKNDKIKVMSTGQAWNVDRIGIF
TPKQVDTDGLNTGEVGWVVCGIKDILGAPVGDTLTHAKGGCEERLPGFQKVKPQVYAGLFPISSDDYENFRDALGKLSLN
DASLFYEPESSSALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPTVVYEVVLNNGDLLYVDSPAKLPAVNDLDEIR
EPIARCNILVPAEYLGNVISLCIEKRGTQVDMIYHGNQVALTYDIPMSEVVLDFFDRLKSTSRGYASLDYNFQRYELSNM
VRVDVLINAERVDALAIITHHDNAQGRGRLLVEKMKEFIPRQMFDIAIQAAIGGHIIARSTVKQLRKNVIAKCYGGDISR
KKKLLKKQKDGKKRMKQIGNVELPQEAFLAILHVGKD
>Mature_597_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMAAQVLDSMDLERERGITIKAQSVTLNYTANDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALEMDLEVVPILNKIDLPAADPDRVAEEIEDIVGIEAMEATR
CSAKTGLGVDAVLETIVKCIPAPEGNPEGPTQALIIDSWFDNYLGVVSLVRVKHGSLKKNDKIKVMSTGQAWNVDRIGIF
TPKQVDTDGLNTGEVGWVVCGIKDILGAPVGDTLTHAKGGCEERLPGFQKVKPQVYAGLFPISSDDYENFRDALGKLSLN
DASLFYEPESSSALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPTVVYEVVLNNGDLLYVDSPAKLPAVNDLDEIR
EPIARCNILVPAEYLGNVISLCIEKRGTQVDMIYHGNQVALTYDIPMSEVVLDFFDRLKSTSRGYASLDYNFQRYELSNM
VRVDVLINAERVDALAIITHHDNAQGRGRLLVEKMKEFIPRQMFDIAIQAAIGGHIIARSTVKQLRKNVIAKCYGGDISR
KKKLLKKQKDGKKRMKQIGNVELPQEAFLAILHVGKD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=47.2545757071547, Blast_Score=588, Evalue=1e-168,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=45.5223880597015, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI25306283, Length=149, Percent_Identity=42.9530201342282, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI19923640, Length=149, Percent_Identity=42.2818791946309, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI25306287, Length=149, Percent_Identity=42.2818791946309, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=40.9722222222222, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI18390331, Length=150, Percent_Identity=36, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=44.1441441441441, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=44.1441441441441, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=44.1441441441441, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI53729339, Length=239, Percent_Identity=27.6150627615063, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI53729337, Length=239, Percent_Identity=27.6150627615063, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI217272894, Length=167, Percent_Identity=33.5329341317365, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI217272892, Length=167, Percent_Identity=33.5329341317365, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1788922, Length=597, Percent_Identity=83.4170854271357, Blast_Score=1005, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=510, Percent_Identity=28.0392156862745, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI1789738, Length=153, Percent_Identity=35.2941176470588, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=32.0512820512821, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789559, Length=224, Percent_Identity=31.6964285714286, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790412, Length=332, Percent_Identity=25.9036144578313, Blast_Score=62, Evalue=9e-11,
Organism=Escherichia coli, GI1789737, Length=332, Percent_Identity=25.9036144578313, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=40.327868852459, Blast_Score=479, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17556745, Length=157, Percent_Identity=34.3949044585987, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=34.9315068493151, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=170, Percent_Identity=35.2941176470588, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=37.7777777777778, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=37.7777777777778, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.1724137931034, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=237, Percent_Identity=28.6919831223629, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=610, Percent_Identity=45.0819672131148, Blast_Score=523, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6323098, Length=183, Percent_Identity=34.9726775956284, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=145, Percent_Identity=42.0689655172414, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=145, Percent_Identity=42.0689655172414, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=38.2608695652174, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=39.3103448275862, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=340, Percent_Identity=26.4705882352941, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=171, Percent_Identity=29.2397660818713, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319594, Length=171, Percent_Identity=29.2397660818713, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=43.4276206322795, Blast_Score=526, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24582462, Length=154, Percent_Identity=38.3116883116883, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=44.6043165467626, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=39.5973154362416, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585709, Length=162, Percent_Identity=33.9506172839506, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24585711, Length=162, Percent_Identity=33.9506172839506, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24585713, Length=162, Percent_Identity=33.9506172839506, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=35.3383458646617, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28572034, Length=223, Percent_Identity=29.1479820627803, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281363316, Length=329, Percent_Identity=25.2279635258359, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17864358, Length=329, Percent_Identity=25.2279635258359, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_ALISL (B6EKN1)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263875.1
- GeneID:   6988742
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2527
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 65906; Mature: 65906

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMAAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LNYTANDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAL
EEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPILNKIDLPAADPDRVAEEIEDIVGIEAMEATRCSAKTGLGVDAVLETIVKCI
HCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHC
PAPEGNPEGPTQALIIDSWFDNYLGVVSLVRVKHGSLKKNDKIKVMSTGQAWNVDRIGIF
CCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEC
TPKQVDTDGLNTGEVGWVVCGIKDILGAPVGDTLTHAKGGCEERLPGFQKVKPQVYAGLF
CCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCCHHHHCCCCHHHHCCCHHHCCCHHHEEEE
PISSDDYENFRDALGKLSLNDASLFYEPESSSALGFGFRCGFLGMLHMEIIQERLEREYD
CCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVVLNNGDLLYVDSPAKLPAVNDLDEIREPIARCNILVPAEYLGNVIS
CEEEECCHHEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHCEEEECHHHHHHHHH
LCIEKRGTQVDMIYHGNQVALTYDIPMSEVVLDFFDRLKSTSRGYASLDYNFQRYELSNM
HHHHCCCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCE
VRVDVLINAERVDALAIITHHDNAQGRGRLLVEKMKEFIPRQMFDIAIQAAIGGHIIARS
EEEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
TVKQLRKNVIAKCYGGDISRKKKLLKKQKDGKKRMKQIGNVELPQEAFLAILHVGKD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMAAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LNYTANDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAL
EEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPILNKIDLPAADPDRVAEEIEDIVGIEAMEATRCSAKTGLGVDAVLETIVKCI
HCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHC
PAPEGNPEGPTQALIIDSWFDNYLGVVSLVRVKHGSLKKNDKIKVMSTGQAWNVDRIGIF
CCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEC
TPKQVDTDGLNTGEVGWVVCGIKDILGAPVGDTLTHAKGGCEERLPGFQKVKPQVYAGLF
CCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCCHHHHCCCCHHHHCCCHHHCCCHHHEEEE
PISSDDYENFRDALGKLSLNDASLFYEPESSSALGFGFRCGFLGMLHMEIIQERLEREYD
CCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVVLNNGDLLYVDSPAKLPAVNDLDEIREPIARCNILVPAEYLGNVIS
CEEEECCHHEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHCEEEECHHHHHHHHH
LCIEKRGTQVDMIYHGNQVALTYDIPMSEVVLDFFDRLKSTSRGYASLDYNFQRYELSNM
HHHHCCCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCE
VRVDVLINAERVDALAIITHHDNAQGRGRLLVEKMKEFIPRQMFDIAIQAAIGGHIIARS
EEEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
TVKQLRKNVIAKCYGGDISRKKKLLKKQKDGKKRMKQIGNVELPQEAFLAILHVGKD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA