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The map label for this gene is relA [H]

Identifier: 209695936

GI number: 209695936

Start: 2697759

End: 2699996

Strand: Reverse

Name: relA [H]

Synonym: VSAL_I2518

Alternate gene names: 209695936

Gene position: 2699996-2697759 (Counterclockwise)

Preceding gene: 209695937

Following gene: 209695935

Centisome position: 81.2

GC content: 41.78

Gene sequence:

>2238_bases
ATGGTCGCAGTTCGCGGAGCACATCTTAAGGAAAATGAAACGTTTGAGTTAGCTGAATGGATAATCAGCCTTGAACAAGA
TAAGAGCACCTCTAAAAAACTGACAGAGGTGTATCAACAATGCCAAGAGTTAGTGGATTCCCATGAAAATGGTCCACTCT
TGTTGTGGCGTGGTCGAGAGATGATAGAAATCTTGATCACCCTAAGCATGGATAAGCCGACTTTAATTGCAGCTCAGTTA
TTTCCTTTAGTTTCATCTGGTGTGTTAGACAGAGAAACGTTAACTGAAAACTACAATAAAGAAATAGAAAAACTGATTGA
TGGCGTTGAAGAGATGGACGCGATTGGTCAGTTGAATGCGTCTGTTGGGGAAGAGTCAGCCTCTTCCCAAGTCGATAATA
TTCGTCGAATGTTATTGGCTATGGTTGATGATTTTCGTTGTGTGATCATTAAATTAGCTGAGCGTATTTGTAATTTACGT
GAAGTGAAAAATGAACCTGAAGACGTTCGCCGAGCAGCGGCTAAAGAATGTAATAATCTTTATGCTCCTTTAGCGAACCG
TTTAGGTATTGGTCAGTTGAAATGGGAAATTGAAGATTACTCTTTCCGTTATCAGCATCCTGATACCTATAAGAAAATAG
CAAAACAATTGTCTGAGCGACGTATTGTTCGTGAACAATATATCCATGATTTTGTCACGGGGTTAAGTGATGAAATGGCG
GGTTATAACATCAATGCCGAAGTCAGTGGGCGTCCAAAGCACATCTACAGTATCTGGCGTAAAATGCAGAAGAAGAGCTT
GGAATTTGATGAATTGTTTGATGTTCGAGCGGTTCGAATTATTGCGGATAAACTGCAAGATTGTTATGCCGCATTAGGGG
TTGTACACACGAAGTTTAAGCACTTACCAAGTGAATTTGACGACTACGTTGCAAACCCAAAACCGAATGGCTATCAATCT
ATTCATACGGTTGTTTTAGGCCCTGAAGGGAAAACCATTGAAATCCAGATCCGAACAAAGGATATGCACGAAGACTCAGA
ACTAGGGGTTGCTGCGCACTGGAAATACAAAGAAGGCTCATCAAACAGCAGTCGTACTGGTTATGATGAGAAGATTACGT
GGCTACGTAAACTGCTTGATTGGCAAGAAGAGATGTCAGATTCTGGTGAAATGCTGGATGAATTACGAAGTCAAGTGTTT
GATGATCGTGTTTATGCCTTTACCCCTCGTGGTGATGTTGTTGATCTACCAATGGGCGCGACACCTTTAGATTTTGCGTA
TCATATTCACTCAGAAGTAGGGCACCGATGTATTGGTGCGAAAGTGGGTGGACGAATCGTGCCATTTACCCATAACCTTG
CAATGGGTGACCAAGTTGAAATTATTACCCAAAAAGAACCGAACCCTTCTCGAGATTGGTTAAACCCAACCTTGGGTTTT
GTGAACTCTGGCCGTGCTCGTGCAAAGATCAATGCCTGGTTTAGAAAACAGAGTCGTGAAAAAAACCTTGATGCAGGTAA
AGAGATTTTAGAAGTTGAATTAGCAAAAATTGGCGCAACGTTAAAAGATGCAGAGCAATACGCACTTAAGCGCTTTAATG
TGAATTCAACGGATGAGTTGTTTGCTGGTGTTGGTAGTGGCGACTTACGTATTAACCAAGTGATTAATCACATTAATGCG
TTAGTGAATAAACCAACCGCTGAGCAAGAAGATCAAAAAGCGTTAGAGCAATTGCAAGAATCTGAAGCGAAGGCACAAGC
ACAAGCTCAGGCGCAGAATAAAAAACGCAATGATGCAGTGGTGGTTGAAGGTGTCGATAATTTAATGACGCACCTAGCAC
GCTGTTGTCAGCCGATCCCTGGTGATCCTATCGCGGGGTACATAACTCAAGGTCGTGGTATTTCAGTTCACCGCAGTGAT
TGTGATCAGCTTGATGAATTACGTCATCATGCTGCAGAACGAATTATCGACACGGTGTGGGGGAGTGGTTTTGTTGGCTC
TTATATATTGACGTTAAGAGTCACAGCCATGGATCGTTCTGGTTTATTGAAGGATCTTACCAGTCTGTTTGCGAATGAAA
AAGTAAAAGTGTCTGGCATGAAGAGCCGTAATGATTACAAAAAGCAGCTGACTATTATGGATTTTGATCTTGAAGTGTTC
AGTGCTGACATCCTTAGTCGATTAATTACTCGTGTTGAACAAGTAAAAGATGTCATGGAAGCCTCTCGATTATCATAG

Upstream 100 bases:

>100_bases
GAATCTATGGCATTGTTTGAGCGATAACTGGCAGAAATCGTCAGGTAAGCGAAATGGAGCCAAGCAGAATCATTATAAAA
TTGGATTATAGGAAACGGAC

Downstream 100 bases:

>100_bases
TAAAACAGAGAAAATAAATTGATGTAGATTTTCTTTAAGTTATTGATAATTAAAACAGATAAAAAATAGAGCCGAATATT
AGCGATGATATTCGGCTTTT

Product: GTP pyrophosphokinase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 745; Mature: 745

Protein sequence:

>745_residues
MVAVRGAHLKENETFELAEWIISLEQDKSTSKKLTEVYQQCQELVDSHENGPLLLWRGREMIEILITLSMDKPTLIAAQL
FPLVSSGVLDRETLTENYNKEIEKLIDGVEEMDAIGQLNASVGEESASSQVDNIRRMLLAMVDDFRCVIIKLAERICNLR
EVKNEPEDVRRAAAKECNNLYAPLANRLGIGQLKWEIEDYSFRYQHPDTYKKIAKQLSERRIVREQYIHDFVTGLSDEMA
GYNINAEVSGRPKHIYSIWRKMQKKSLEFDELFDVRAVRIIADKLQDCYAALGVVHTKFKHLPSEFDDYVANPKPNGYQS
IHTVVLGPEGKTIEIQIRTKDMHEDSELGVAAHWKYKEGSSNSSRTGYDEKITWLRKLLDWQEEMSDSGEMLDELRSQVF
DDRVYAFTPRGDVVDLPMGATPLDFAYHIHSEVGHRCIGAKVGGRIVPFTHNLAMGDQVEIITQKEPNPSRDWLNPTLGF
VNSGRARAKINAWFRKQSREKNLDAGKEILEVELAKIGATLKDAEQYALKRFNVNSTDELFAGVGSGDLRINQVINHINA
LVNKPTAEQEDQKALEQLQESEAKAQAQAQAQNKKRNDAVVVEGVDNLMTHLARCCQPIPGDPIAGYITQGRGISVHRSD
CDQLDELRHHAAERIIDTVWGSGFVGSYILTLRVTAMDRSGLLKDLTSLFANEKVKVSGMKSRNDYKKQLTIMDFDLEVF
SADILSRLITRVEQVKDVMEASRLS

Sequences:

>Translated_745_residues
MVAVRGAHLKENETFELAEWIISLEQDKSTSKKLTEVYQQCQELVDSHENGPLLLWRGREMIEILITLSMDKPTLIAAQL
FPLVSSGVLDRETLTENYNKEIEKLIDGVEEMDAIGQLNASVGEESASSQVDNIRRMLLAMVDDFRCVIIKLAERICNLR
EVKNEPEDVRRAAAKECNNLYAPLANRLGIGQLKWEIEDYSFRYQHPDTYKKIAKQLSERRIVREQYIHDFVTGLSDEMA
GYNINAEVSGRPKHIYSIWRKMQKKSLEFDELFDVRAVRIIADKLQDCYAALGVVHTKFKHLPSEFDDYVANPKPNGYQS
IHTVVLGPEGKTIEIQIRTKDMHEDSELGVAAHWKYKEGSSNSSRTGYDEKITWLRKLLDWQEEMSDSGEMLDELRSQVF
DDRVYAFTPRGDVVDLPMGATPLDFAYHIHSEVGHRCIGAKVGGRIVPFTHNLAMGDQVEIITQKEPNPSRDWLNPTLGF
VNSGRARAKINAWFRKQSREKNLDAGKEILEVELAKIGATLKDAEQYALKRFNVNSTDELFAGVGSGDLRINQVINHINA
LVNKPTAEQEDQKALEQLQESEAKAQAQAQAQNKKRNDAVVVEGVDNLMTHLARCCQPIPGDPIAGYITQGRGISVHRSD
CDQLDELRHHAAERIIDTVWGSGFVGSYILTLRVTAMDRSGLLKDLTSLFANEKVKVSGMKSRNDYKKQLTIMDFDLEVF
SADILSRLITRVEQVKDVMEASRLS
>Mature_745_residues
MVAVRGAHLKENETFELAEWIISLEQDKSTSKKLTEVYQQCQELVDSHENGPLLLWRGREMIEILITLSMDKPTLIAAQL
FPLVSSGVLDRETLTENYNKEIEKLIDGVEEMDAIGQLNASVGEESASSQVDNIRRMLLAMVDDFRCVIIKLAERICNLR
EVKNEPEDVRRAAAKECNNLYAPLANRLGIGQLKWEIEDYSFRYQHPDTYKKIAKQLSERRIVREQYIHDFVTGLSDEMA
GYNINAEVSGRPKHIYSIWRKMQKKSLEFDELFDVRAVRIIADKLQDCYAALGVVHTKFKHLPSEFDDYVANPKPNGYQS
IHTVVLGPEGKTIEIQIRTKDMHEDSELGVAAHWKYKEGSSNSSRTGYDEKITWLRKLLDWQEEMSDSGEMLDELRSQVF
DDRVYAFTPRGDVVDLPMGATPLDFAYHIHSEVGHRCIGAKVGGRIVPFTHNLAMGDQVEIITQKEPNPSRDWLNPTLGF
VNSGRARAKINAWFRKQSREKNLDAGKEILEVELAKIGATLKDAEQYALKRFNVNSTDELFAGVGSGDLRINQVINHINA
LVNKPTAEQEDQKALEQLQESEAKAQAQAQAQNKKRNDAVVVEGVDNLMTHLARCCQPIPGDPIAGYITQGRGISVHRSD
CDQLDELRHHAAERIIDTVWGSGFVGSYILTLRVTAMDRSGLLKDLTSLFANEKVKVSGMKSRNDYKKQLTIMDFDLEVF
SADILSRLITRVEQVKDVMEASRLS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1789147, Length=746, Percent_Identity=62.7345844504021, Blast_Score=991, Evalue=0.0,
Organism=Escherichia coli, GI1790082, Length=692, Percent_Identity=31.7919075144509, Blast_Score=387, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 84396; Mature: 84396

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVRGAHLKENETFELAEWIISLEQDKSTSKKLTEVYQQCQELVDSHENGPLLLWRGRE
CCEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHH
MIEILITLSMDKPTLIAAQLFPLVSSGVLDRETLTENYNKEIEKLIDGVEEMDAIGQLNA
HHHHHHEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVGEESASSQVDNIRRMLLAMVDDFRCVIIKLAERICNLREVKNEPEDVRRAAAKECNNL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YAPLANRLGIGQLKWEIEDYSFRYQHPDTYKKIAKQLSERRIVREQYIHDFVTGLSDEMA
HHHHHHCCCCCEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
GYNINAEVSGRPKHIYSIWRKMQKKSLEFDELFDVRAVRIIADKLQDCYAALGVVHTKFK
CCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLPSEFDDYVANPKPNGYQSIHTVVLGPEGKTIEIQIRTKDMHEDSELGVAAHWKYKEGS
HCCHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEEEECCCC
SNSSRTGYDEKITWLRKLLDWQEEMSDSGEMLDELRSQVFDDRVYAFTPRGDVVDLPMGA
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCC
TPLDFAYHIHSEVGHRCIGAKVGGRIVPFTHNLAMGDQVEIITQKEPNPSRDWLNPTLGF
CCHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCCCEEEEECCCCCCCHHHCCCHHHH
VNSGRARAKINAWFRKQSREKNLDAGKEILEVELAKIGATLKDAEQYALKRFNVNSTDEL
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHH
FAGVGSGDLRINQVINHINALVNKPTAEQEDQKALEQLQESEAKAQAQAQAQNKKRNDAV
HHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VVEGVDNLMTHLARCCQPIPGDPIAGYITQGRGISVHRSDCDQLDELRHHAAERIIDTVW
EEECHHHHHHHHHHHCCCCCCCCCCHHEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHC
GSGFVGSYILTLRVTAMDRSGLLKDLTSLFANEKVKVSGMKSRNDYKKQLTIMDFDLEVF
CCCHHHHEEEHHEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCHHHH
SADILSRLITRVEQVKDVMEASRLS
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVAVRGAHLKENETFELAEWIISLEQDKSTSKKLTEVYQQCQELVDSHENGPLLLWRGRE
CCEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHH
MIEILITLSMDKPTLIAAQLFPLVSSGVLDRETLTENYNKEIEKLIDGVEEMDAIGQLNA
HHHHHHEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVGEESASSQVDNIRRMLLAMVDDFRCVIIKLAERICNLREVKNEPEDVRRAAAKECNNL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YAPLANRLGIGQLKWEIEDYSFRYQHPDTYKKIAKQLSERRIVREQYIHDFVTGLSDEMA
HHHHHHCCCCCEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
GYNINAEVSGRPKHIYSIWRKMQKKSLEFDELFDVRAVRIIADKLQDCYAALGVVHTKFK
CCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLPSEFDDYVANPKPNGYQSIHTVVLGPEGKTIEIQIRTKDMHEDSELGVAAHWKYKEGS
HCCHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEEEECCCC
SNSSRTGYDEKITWLRKLLDWQEEMSDSGEMLDELRSQVFDDRVYAFTPRGDVVDLPMGA
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCC
TPLDFAYHIHSEVGHRCIGAKVGGRIVPFTHNLAMGDQVEIITQKEPNPSRDWLNPTLGF
CCHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCCCEEEEECCCCCCCHHHCCCHHHH
VNSGRARAKINAWFRKQSREKNLDAGKEILEVELAKIGATLKDAEQYALKRFNVNSTDEL
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHH
FAGVGSGDLRINQVINHINALVNKPTAEQEDQKALEQLQESEAKAQAQAQAQNKKRNDAV
HHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VVEGVDNLMTHLARCCQPIPGDPIAGYITQGRGISVHRSDCDQLDELRHHAAERIIDTVW
EEECHHHHHHHHHHHCCCCCCCCCCHHEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHC
GSGFVGSYILTLRVTAMDRSGLLKDLTSLFANEKVKVSGMKSRNDYKKQLTIMDFDLEVF
CCCHHHHEEEHHEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCHHHH
SADILSRLITRVEQVKDVMEASRLS
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7928955 [H]