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The map label for this gene is rumA

Identifier: 209695937

GI number: 209695937

Start: 2700070

End: 2701386

Strand: Reverse

Name: rumA

Synonym: VSAL_I2519

Alternate gene names: 209695937

Gene position: 2701386-2700070 (Counterclockwise)

Preceding gene: 209695939

Following gene: 209695936

Centisome position: 81.24

GC content: 38.42

Gene sequence:

>1317_bases
ATGGCGCAATTCTTCAAACCGAAAAAGAAAGCTTCGGTAAACACAAAACACCTATCTGTAGATGTGGTTCGATTAGACCA
CAATAGTGCGGGTATTGCTTTTGTGGATAAAAAACCGGTGTTTATCGAAGGCGCCTTACCTGAAGAACAAGCGATAATTC
AATTCATTGAGCAAAAGAAACAATATTCTCGTGCTAAATTGATTAAATTGACAAAAAAAAGCAGTAAACGCCAAGCTCCA
ATTTGTCAGCATTATGACGATTGTGGTGGTTGTAATCTTCAACATTTACAGCATGCAGAGCAAATCATTGCTAAAAATGA
CAAGCTGCAAGAGTTAATGAAAAAACAAGGTGTTGATGCTTGTGATGTTGCTGAACCCATTCTTGCGAATGAGCATGATT
ACCGACGCCGAGCTCGTATTAGTTTAATCATGAATAAACAAACGAATCAATTGGATTTTGGTTTTAGAAAAAAACAGAGC
AAAGAAATTGTTAATGTTCGTCATTGTCCCGTTTTAGTAAAAGAACTCGATCAGCATTTAGAATCGCTATACACCTTACT
TAATCAGCTTAAAGGTAAGCGACATTTAGGTCATGTTGAATTAGTTCAAGCAGATAACGGAAGTGTATTGCTCATTCGCC
ATGTCGCTGCATTTAATGAAAAAGATCAACAAGCTTTACTTCATTATTGTGAAGAACGAGATTTGATCCTATACCTAATG
CCTGAGTCAGATGTATTGAATCACGTATATGGCGAAGAACCGTATTATGTCATTGATGAGGTGAAGATTTATTTTACGCC
TAAAGATTTCATTCAAGTTAATGCCTCAATTAATGACAGCATGGTAAATCAAGCATTAACTTGGTTGGATCTCAATGCGG
ACGATTGTGTACTCGATTTATTCTGTGGCTTAGGTAATTTCAGTTTGCCATTGGCACAGAAAGTAAAAACAGTCGTAGGT
ATTGAAGGCGTTGAAGAAATGGTTAAGCGCGCTCAATCTAACGCAAAACGAAATGAATTAGATAATGTGACGTTTTATCA
AGCAAATTTAGAAGAGCCGATTGATCAGCAAGTGTGGGCAAGCACAAAGTTTACAAAGATTTTACTGGATCCTGCCAGAG
CAGGGGCTGCAGGAGTGATGCAAACTGTGGCAAAATTGAAGCCTGAAACCGTCGTTTATGTATCGTGCAACCCAGCAACA
CTTGCACGCGATAGCCAATTGCTTATTCAACGCGGTTACAAGCTTACTAGGCTAGGTATGCTTGATATGTTCCCACATAC
GGGACATTTAGAATCTATGGCATTGTTTGAGCGATAA

Upstream 100 bases:

>100_bases
TTGTTCAATCTATAAACCTGAAGCTTAGGCACAAATGAGGACTGCATCTTCAAATCGTTTTGGTATAATAGTGCTAATTT
TTGTAAATCAGGCAGTAATC

Downstream 100 bases:

>100_bases
CTGGCAGAAATCGTCAGGTAAGCGAAATGGAGCCAAGCAGAATCATTATAAAATTGGATTATAGGAAACGGACATGGTCG
CAGTTCGCGGAGCACATCTT

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MAQFFKPKKKASVNTKHLSVDVVRLDHNSAGIAFVDKKPVFIEGALPEEQAIIQFIEQKKQYSRAKLIKLTKKSSKRQAP
ICQHYDDCGGCNLQHLQHAEQIIAKNDKLQELMKKQGVDACDVAEPILANEHDYRRRARISLIMNKQTNQLDFGFRKKQS
KEIVNVRHCPVLVKELDQHLESLYTLLNQLKGKRHLGHVELVQADNGSVLLIRHVAAFNEKDQQALLHYCEERDLILYLM
PESDVLNHVYGEEPYYVIDEVKIYFTPKDFIQVNASINDSMVNQALTWLDLNADDCVLDLFCGLGNFSLPLAQKVKTVVG
IEGVEEMVKRAQSNAKRNELDNVTFYQANLEEPIDQQVWASTKFTKILLDPARAGAAGVMQTVAKLKPETVVYVSCNPAT
LARDSQLLIQRGYKLTRLGMLDMFPHTGHLESMALFER

Sequences:

>Translated_438_residues
MAQFFKPKKKASVNTKHLSVDVVRLDHNSAGIAFVDKKPVFIEGALPEEQAIIQFIEQKKQYSRAKLIKLTKKSSKRQAP
ICQHYDDCGGCNLQHLQHAEQIIAKNDKLQELMKKQGVDACDVAEPILANEHDYRRRARISLIMNKQTNQLDFGFRKKQS
KEIVNVRHCPVLVKELDQHLESLYTLLNQLKGKRHLGHVELVQADNGSVLLIRHVAAFNEKDQQALLHYCEERDLILYLM
PESDVLNHVYGEEPYYVIDEVKIYFTPKDFIQVNASINDSMVNQALTWLDLNADDCVLDLFCGLGNFSLPLAQKVKTVVG
IEGVEEMVKRAQSNAKRNELDNVTFYQANLEEPIDQQVWASTKFTKILLDPARAGAAGVMQTVAKLKPETVVYVSCNPAT
LARDSQLLIQRGYKLTRLGMLDMFPHTGHLESMALFER
>Mature_437_residues
AQFFKPKKKASVNTKHLSVDVVRLDHNSAGIAFVDKKPVFIEGALPEEQAIIQFIEQKKQYSRAKLIKLTKKSSKRQAPI
CQHYDDCGGCNLQHLQHAEQIIAKNDKLQELMKKQGVDACDVAEPILANEHDYRRRARISLIMNKQTNQLDFGFRKKQSK
EIVNVRHCPVLVKELDQHLESLYTLLNQLKGKRHLGHVELVQADNGSVLLIRHVAAFNEKDQQALLHYCEERDLILYLMP
ESDVLNHVYGEEPYYVIDEVKIYFTPKDFIQVNASINDSMVNQALTWLDLNADDCVLDLFCGLGNFSLPLAQKVKTVVGI
EGVEEMVKRAQSNAKRNELDNVTFYQANLEEPIDQQVWASTKFTKILLDPARAGAAGVMQTVAKLKPETVVYVSCNPATL
ARDSQLLIQRGYKLTRLGMLDMFPHTGHLESMALFER

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=207, Percent_Identity=34.7826086956522, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI51173878, Length=207, Percent_Identity=34.7826086956522, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI269784680, Length=211, Percent_Identity=28.436018957346, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI269784676, Length=211, Percent_Identity=28.436018957346, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI20149688, Length=211, Percent_Identity=28.436018957346, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI269784678, Length=211, Percent_Identity=28.436018957346, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789148, Length=438, Percent_Identity=48.8584474885845, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1787083, Length=395, Percent_Identity=22.5316455696203, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790403, Length=180, Percent_Identity=32.7777777777778, Blast_Score=89, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24666579, Length=203, Percent_Identity=28.0788177339901, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_ALISL (B6EKM3)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263867.1
- ProteinModelPortal:   B6EKM3
- GeneID:   6989086
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2519
- HOGENOM:   HBG690163
- OMA:   VVFVEGA
- ProtClustDB:   PRK13168
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 49810; Mature: 49679

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 396-396 BINDING 272-272 BINDING 301-301 BINDING 306-306 BINDING 322-322 BINDING 349-349 BINDING 370-370

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFFKPKKKASVNTKHLSVDVVRLDHNSAGIAFVDKKPVFIEGALPEEQAIIQFIEQKK
CCCCCCCCHHCCCCCEEEEEEEEEEECCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHH
QYSRAKLIKLTKKSSKRQAPICQHYDDCGGCNLQHLQHAEQIIAKNDKLQELMKKQGVDA
HHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCH
CDVAEPILANEHDYRRRARISLIMNKQTNQLDFGFRKKQSKEIVNVRHCPVLVKELDQHL
HHHCCHHHCCCHHHHHHHEEEEEECCCCCCCCCCHHHHCCHHHHCHHHHHHHHHHHHHHH
ESLYTLLNQLKGKRHLGHVELVQADNGSVLLIRHVAAFNEKDQQALLHYCEERDLILYLM
HHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEHHHCCCCHHHHHHHHHCCCCEEEEEC
PESDVLNHVYGEEPYYVIDEVKIYFTPKDFIQVNASINDSMVNQALTWLDLNADDCVLDL
CCHHHHHHHCCCCCEEEEEEEEEEECCHHHEEEECCCCHHHHHHHHHEEECCCCHHHHHH
FCGLGNFSLPLAQKVKTVVGIEGVEEMVKRAQSNAKRNELDNVTFYQANLEEPIDQQVWA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCCCHHHHHHC
STKFTKILLDPARAGAAGVMQTVAKLKPETVVYVSCNPATLARDSQLLIQRGYKLTRLGM
CCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHHCCCCEEEECH
LDMFPHTGHLESMALFER
HHCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
AQFFKPKKKASVNTKHLSVDVVRLDHNSAGIAFVDKKPVFIEGALPEEQAIIQFIEQKK
CCCCCCCHHCCCCCEEEEEEEEEEECCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHH
QYSRAKLIKLTKKSSKRQAPICQHYDDCGGCNLQHLQHAEQIIAKNDKLQELMKKQGVDA
HHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCH
CDVAEPILANEHDYRRRARISLIMNKQTNQLDFGFRKKQSKEIVNVRHCPVLVKELDQHL
HHHCCHHHCCCHHHHHHHEEEEEECCCCCCCCCCHHHHCCHHHHCHHHHHHHHHHHHHHH
ESLYTLLNQLKGKRHLGHVELVQADNGSVLLIRHVAAFNEKDQQALLHYCEERDLILYLM
HHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEHHHCCCCHHHHHHHHHCCCCEEEEEC
PESDVLNHVYGEEPYYVIDEVKIYFTPKDFIQVNASINDSMVNQALTWLDLNADDCVLDL
CCHHHHHHHCCCCCEEEEEEEEEEECCHHHEEEECCCCHHHHHHHHHEEECCCCHHHHHH
FCGLGNFSLPLAQKVKTVVGIEGVEEMVKRAQSNAKRNELDNVTFYQANLEEPIDQQVWA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCCCHHHHHHC
STKFTKILLDPARAGAAGVMQTVAKLKPETVVYVSCNPATLARDSQLLIQRGYKLTRLGM
CCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHHCCCCEEEECH
LDMFPHTGHLESMALFER
HHCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA