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The map label for this gene is pyrG

Identifier: 209695933

GI number: 209695933

Start: 2693718

End: 2695358

Strand: Reverse

Name: pyrG

Synonym: VSAL_I2515

Alternate gene names: 209695933

Gene position: 2695358-2693718 (Counterclockwise)

Preceding gene: 209695934

Following gene: 209695932

Centisome position: 81.06

GC content: 42.54

Gene sequence:

>1641_bases
ATGACGACGAATTACATTTTTGTAACTGGCGGGGTTGTATCCTCTCTAGGTAAAGGTATTGCAGCAGCATCATTAGCAGC
TATTCTTGAAGCTCGTGGCTTAAAAGTCACTATGATGAAGCTTGACCCATACATCAACGTTGATCCAGGCACAATGAGCC
CAACTCAACACGGTGAAGTATTCGTCACGGAAGACGGCGCAGAAACTGATCTAGATTTAGGTCACTACGAGCGCTTCATT
CGCACCAAAATGACTAAACGTAATAACTTTACGGCTGGTCGAGTTTACGCTGATGTTCTACGTAAAGAACGTCGCGGTGA
CTACTTAGGTGCAACTATTCAAGTTATCCCTCATATTACGAATGCGATTAAAGAACGTGTTATTTCTGGCGCTGCAGGCC
ATGATATCGCACTCGTTGAAGTTGGTGGCACAGTTGGTGATATTGAATCTCTGCCATTCATGGAAGCGATTCGTCAGCTT
GCTGTTGAGCTAGGTCGTGAACACACAATGTTTATGCACTTAACACTTGTTCCTTACTTGGTTGCAGCAGGCGAGCTTAA
AACTAAGCCGACTCAACACTCAGTAAAAGAGCTACTGTCTATTGGTATCCAACCAGATATCTTAGTTTGTCGTTCAGATC
GTGTTATTCCAGCAAACGAACGTAAGAAAATTGCTCTTTTCTGTAATGTACAAGAAAAAGCAGTTATCTCAATGAAAGAC
GTAGATTCAATCTACAAAATCCCACAATTGATCCGAGCGCAAGGTACTGATGATTTAGTGTGTCAACGCTTTGGTATTAC
CGCTCCTGCCGCTGATCTTTCTGAGTGGGAACAAGTAATTTATGAAGAAGCGAACCCGACTGGTGAAGTTACTATCGGTA
TGGTTGGTAAATACATTGAATTACCAGATGCTTACAAGTCAGTAAACGAAGCATTAAAACATGCGGGCTTGAAAAATCGC
TTGAGCGTCACTATTAAATATATAGATTCACAAGATGTTGAATCTAAAGGTACTGAAATCCTAGAAGGTCTAGATGCAAT
TCTAGTTCCTGGTGGTTTTGGTGACCGTGGTATTGAAGGTAAGATCCTTGCTGCTCAATACGCTCGTGAAAATGCAGTAC
CATATTTAGGAATTTGTCTGGGTATGCAAGTTGCGCTAATTGATTACGCGCGTAACGTTGCTAATATGGAAGGGGCTCAC
TCTTCTGAATTCTCTAAAGACACGAAATTCCCTGTTGTTGGTCTAATCACAGAGTGGATTGATGGCGACGGTAATGTTGA
AGAGCGTACTGAGAAATCAGATCTAGGCGGTACAATGCGTCTTGGTTCTCAACTATGTCATCTTGCTAAAGGCTCTAAAG
CTCGTGAGCTTTACGGTAACGCAACAGTTGAAGAGCGTCATCGTCACCGCTATGAAGTAAATAATAATTTACTTCCGCAG
CTTGAAAAAGCCGGTCTTAAGATCTCTGGCTTATCTGCTGATAAGAAGCTTGTGGAGATAATTGAAATCCCTAACCACCC
ATGGTTTGTGGCTGCTCAATTCCACCCTGAATTCACATCAACACCTCGTGATGGACACCCATTATTTGAAGGTTTTGTTA
AAGCAGCAGGCGAAAATGCCCGCGGTGAATTCGAAAAGTAA

Upstream 100 bases:

>100_bases
GATACTTTTCACGTTGTGGCCATAAAGCGGTTCTGGTATATTCATTTCCCGTCCAGATAAAATCCATTTTATTCCTCAAT
ACACCAATTTCAGGTAACGT

Downstream 100 bases:

>100_bases
TAGGATTTACGGGTAGCTGTCCAATGTTGTGGCAGCTATTTTTAGCTTTTAAATTAAAGAAAATAGAGGAAACACAATGT
CTAAGATCGTTAAAGTTCTA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHITNAIKERVISGAAGHDIALVEVGGTVGDIESLPFMEAIRQL
AVELGREHTMFMHLTLVPYLVAAGELKTKPTQHSVKELLSIGIQPDILVCRSDRVIPANERKKIALFCNVQEKAVISMKD
VDSIYKIPQLIRAQGTDDLVCQRFGITAPAADLSEWEQVIYEEANPTGEVTIGMVGKYIELPDAYKSVNEALKHAGLKNR
LSVTIKYIDSQDVESKGTEILEGLDAILVPGGFGDRGIEGKILAAQYARENAVPYLGICLGMQVALIDYARNVANMEGAH
SSEFSKDTKFPVVGLITEWIDGDGNVEERTEKSDLGGTMRLGSQLCHLAKGSKARELYGNATVEERHRHRYEVNNNLLPQ
LEKAGLKISGLSADKKLVEIIEIPNHPWFVAAQFHPEFTSTPRDGHPLFEGFVKAAGENARGEFEK

Sequences:

>Translated_546_residues
MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHITNAIKERVISGAAGHDIALVEVGGTVGDIESLPFMEAIRQL
AVELGREHTMFMHLTLVPYLVAAGELKTKPTQHSVKELLSIGIQPDILVCRSDRVIPANERKKIALFCNVQEKAVISMKD
VDSIYKIPQLIRAQGTDDLVCQRFGITAPAADLSEWEQVIYEEANPTGEVTIGMVGKYIELPDAYKSVNEALKHAGLKNR
LSVTIKYIDSQDVESKGTEILEGLDAILVPGGFGDRGIEGKILAAQYARENAVPYLGICLGMQVALIDYARNVANMEGAH
SSEFSKDTKFPVVGLITEWIDGDGNVEERTEKSDLGGTMRLGSQLCHLAKGSKARELYGNATVEERHRHRYEVNNNLLPQ
LEKAGLKISGLSADKKLVEIIEIPNHPWFVAAQFHPEFTSTPRDGHPLFEGFVKAAGENARGEFEK
>Mature_545_residues
TTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIR
TKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHITNAIKERVISGAAGHDIALVEVGGTVGDIESLPFMEAIRQLA
VELGREHTMFMHLTLVPYLVAAGELKTKPTQHSVKELLSIGIQPDILVCRSDRVIPANERKKIALFCNVQEKAVISMKDV
DSIYKIPQLIRAQGTDDLVCQRFGITAPAADLSEWEQVIYEEANPTGEVTIGMVGKYIELPDAYKSVNEALKHAGLKNRL
SVTIKYIDSQDVESKGTEILEGLDAILVPGGFGDRGIEGKILAAQYARENAVPYLGICLGMQVALIDYARNVANMEGAHS
SEFSKDTKFPVVGLITEWIDGDGNVEERTEKSDLGGTMRLGSQLCHLAKGSKARELYGNATVEERHRHRYEVNNNLLPQL
EKAGLKISGLSADKKLVEIIEIPNHPWFVAAQFHPEFTSTPRDGHPLFEGFVKAAGENARGEFEK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=557, Percent_Identity=43.9856373429084, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI28559085, Length=568, Percent_Identity=44.0140845070423, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI28559083, Length=568, Percent_Identity=44.0140845070423, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI221316689, Length=568, Percent_Identity=44.0140845070423, Blast_Score=466, Evalue=1e-131,
Organism=Escherichia coli, GI1789142, Length=538, Percent_Identity=79.9256505576208, Blast_Score=893, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=617, Percent_Identity=38.0875202593193, Blast_Score=422, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6319432, Length=570, Percent_Identity=41.4035087719298, Blast_Score=445, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322563, Length=565, Percent_Identity=41.0619469026549, Blast_Score=439, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24664469, Length=556, Percent_Identity=43.5251798561151, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI21357815, Length=502, Percent_Identity=42.0318725099602, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_ALISL (B6EKL9)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263863.1
- ProteinModelPortal:   B6EKL9
- GeneID:   6988442
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2515
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59899; Mature: 59768

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 379-379 ACT_SITE 515-515 ACT_SITE 517-517

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEV
CCCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NAIKERVISGAAGHDIALVEVGGTVGDIESLPFMEAIRQLAVELGREHTMFMHLTLVPYL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHH
VAAGELKTKPTQHSVKELLSIGIQPDILVCRSDRVIPANERKKIALFCNVQEKAVISMKD
HHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEEECCHHHEEEHHH
VDSIYKIPQLIRAQGTDDLVCQRFGITAPAADLSEWEQVIYEEANPTGEVTIGMVGKYIE
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEC
LPDAYKSVNEALKHAGLKNRLSVTIKYIDSQDVESKGTEILEGLDAILVPGGFGDRGIEG
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCC
KILAAQYARENAVPYLGICLGMQVALIDYARNVANMEGAHSSEFSKDTKFPVVGLITEWI
CEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
DGDGNVEERTEKSDLGGTMRLGSQLCHLAKGSKARELYGNATVEERHRHRYEVNNNLLPQ
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCH
LEKAGLKISGLSADKKLVEIIEIPNHPWFVAAQFHPEFTSTPRDGHPLFEGFVKAAGENA
HHHCCCEEECCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCC
RGEFEK
CCCCCC
>Mature Secondary Structure 
TTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEV
CCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NAIKERVISGAAGHDIALVEVGGTVGDIESLPFMEAIRQLAVELGREHTMFMHLTLVPYL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHH
VAAGELKTKPTQHSVKELLSIGIQPDILVCRSDRVIPANERKKIALFCNVQEKAVISMKD
HHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEEECCHHHEEEHHH
VDSIYKIPQLIRAQGTDDLVCQRFGITAPAADLSEWEQVIYEEANPTGEVTIGMVGKYIE
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEC
LPDAYKSVNEALKHAGLKNRLSVTIKYIDSQDVESKGTEILEGLDAILVPGGFGDRGIEG
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCC
KILAAQYARENAVPYLGICLGMQVALIDYARNVANMEGAHSSEFSKDTKFPVVGLITEWI
CEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
DGDGNVEERTEKSDLGGTMRLGSQLCHLAKGSKARELYGNATVEERHRHRYEVNNNLLPQ
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCH
LEKAGLKISGLSADKKLVEIIEIPNHPWFVAAQFHPEFTSTPRDGHPLFEGFVKAAGENA
HHHCCCEEECCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCC
RGEFEK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA