Click here to switch to the map view.

The map label for this gene is eno

Identifier: 209695932

GI number: 209695932

Start: 2692343

End: 2693641

Strand: Reverse

Name: eno

Synonym: VSAL_I2514

Alternate gene names: 209695932

Gene position: 2693641-2692343 (Counterclockwise)

Preceding gene: 209695933

Following gene: 209695931

Centisome position: 81.01

GC content: 44.42

Gene sequence:

>1299_bases
ATGTCTAAGATCGTTAAAGTTCTAGGTCGTGAAATCATCGATTCTCGTGGTAACCCAACAGTTGAAGCTGAAGTTCATCT
AGAAAGTGGTTTTGTTGGTATGGCTGCTGCTCCATCTGGCGCATCTACTGGTTCTCGTGAAGCTCTTGAGCTACGTGACG
GCGACAAATCTCGTTTCCTAGGTAAAGGTGTTCTTAAAGCTATCGCAGCTGTAAATGGCCCAATCGCAGAAGCTCTAATC
GGCAAAGACGCGAAAAACCAAGCTGAAATCGACCAAATCATGATCGATTTAGATGGCACAGAAAACAAAGCAAAATTTGG
CGCTAACGCAATCCTTGCTGTTTCTCTAGCGAATGCTAAAGCTGCTGCAGCTGCTAAATCTATGCCTCTTTACGCTCACA
TTGCTGAACTAAACGGCACTCCTGGCGTATTCTCTATGCCTCTACCAATGATGAACATCATCAACGGTGGCGAGCACGCT
GATAACAACGTTGATATCCAAGAATTCATGATTCAACCTGTTGGCGCTAAAACGCTTAAAGAAGCGGTACGCATGGGCGC
AGAAGTTTTCCACAACCTAGCTAAAGTTCTTAAGTCTAAAGGCTACAGCACAGCGGTTGGTGATGAAGGCGGTTTCGCTC
CTAACCTTAAATCTAACGCTGAAGCGCTAGAAGTTATCGCAGAAGCTGTTGCAGCTGCTGGTTACGAGCTAGGCAAAGAC
GTTACTCTTGCTATGGACTGTGCTGCTTCTGAGTTCTACAACAAAGAAGACGGTATCTACGACCTTAAAGGCGAAGGTAA
GAAATTTACGGCTGAAGAATTCAACCATTACCTAGCTGGTCTTGTTGAGCAATTCCCAATCGTTTCTATCGAAGATGGTC
TAGACGAGTCTGATTGGGCTGGCTTCAAGCACCAAACTGAACTTCTTGGTGACAAGATTCAACTAGTTGGTGACGATCTA
TTCGTTACAAACACTAAGATTCTTGCTCGTGGTATCGAAGAAGGCATCACTAACTCAATTCTTATCAAGTTCAACCAAAT
CGGTTCACTTACAGAGACTCTAGCTGCAATTAAGATGGCTAAAGATGCTGGTTTCACAGCAGTTATCTCTCACCGTTCTG
GCGAAACTGAAGATGCAACAATTGCTGATCTAGCAGTTGGTACAGCGGCAGGTCAAATCAAAACTGGTTCTATGAGCCGT
TCTGACCGTGTTGCTAAGTACAACCAACTAATCCGTATCGAAGAAGCTCTAGGTGAACTAGCGCCATTCAACGGTCTTAA
AGAAGTTAAAGGTCAATAA

Upstream 100 bases:

>100_bases
GCCCGCGGTGAATTCGAAAAGTAATAGGATTTACGGGTAGCTGTCCAATGTTGTGGCAGCTATTTTTAGCTTTTAAATTA
AAGAAAATAGAGGAAACACA

Downstream 100 bases:

>100_bases
TCTCAATTTTTGAGAATATGACTTAATTCTTTAATCGTTCGATTAGAATTTTGATACAAAAACCGTCTCATTTGAGGCGG
TTTTTTTTATTTCAGCTATA

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSKIVKVLGREIIDSRGNPTVEAEVHLESGFVGMAAAPSGASTGSREALELRDGDKSRFLGKGVLKAIAAVNGPIAEALI
GKDAKNQAEIDQIMIDLDGTENKAKFGANAILAVSLANAKAAAAAKSMPLYAHIAELNGTPGVFSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTLKEAVRMGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYELGKD
VTLAMDCAASEFYNKEDGIYDLKGEGKKFTAEEFNHYLAGLVEQFPIVSIEDGLDESDWAGFKHQTELLGDKIQLVGDDL
FVTNTKILARGIEEGITNSILIKFNQIGSLTETLAAIKMAKDAGFTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRVAKYNQLIRIEEALGELAPFNGLKEVKGQ

Sequences:

>Translated_432_residues
MSKIVKVLGREIIDSRGNPTVEAEVHLESGFVGMAAAPSGASTGSREALELRDGDKSRFLGKGVLKAIAAVNGPIAEALI
GKDAKNQAEIDQIMIDLDGTENKAKFGANAILAVSLANAKAAAAAKSMPLYAHIAELNGTPGVFSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTLKEAVRMGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYELGKD
VTLAMDCAASEFYNKEDGIYDLKGEGKKFTAEEFNHYLAGLVEQFPIVSIEDGLDESDWAGFKHQTELLGDKIQLVGDDL
FVTNTKILARGIEEGITNSILIKFNQIGSLTETLAAIKMAKDAGFTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRVAKYNQLIRIEEALGELAPFNGLKEVKGQ
>Mature_431_residues
SKIVKVLGREIIDSRGNPTVEAEVHLESGFVGMAAAPSGASTGSREALELRDGDKSRFLGKGVLKAIAAVNGPIAEALIG
KDAKNQAEIDQIMIDLDGTENKAKFGANAILAVSLANAKAAAAAKSMPLYAHIAELNGTPGVFSMPLPMMNIINGGEHAD
NNVDIQEFMIQPVGAKTLKEAVRMGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYELGKDV
TLAMDCAASEFYNKEDGIYDLKGEGKKFTAEEFNHYLAGLVEQFPIVSIEDGLDESDWAGFKHQTELLGDKIQLVGDDLF
VTNTKILARGIEEGITNSILIKFNQIGSLTETLAAIKMAKDAGFTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRS
DRVAKYNQLIRIEEALGELAPFNGLKEVKGQ

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI301897477, Length=432, Percent_Identity=53.9351851851852, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI301897469, Length=432, Percent_Identity=53.9351851851852, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=53.2407407407407, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=51.8518518518518, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI301897479, Length=430, Percent_Identity=49.7674418604651, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI169201331, Length=339, Percent_Identity=28.6135693215339, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI169201757, Length=339, Percent_Identity=28.6135693215339, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI239744207, Length=339, Percent_Identity=28.6135693215339, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1789141, Length=433, Percent_Identity=86.8360277136259, Blast_Score=738, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71995829, Length=435, Percent_Identity=52.4137931034483, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17536383, Length=435, Percent_Identity=52.4137931034483, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32563855, Length=194, Percent_Identity=46.3917525773196, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=49.1916859122402, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323985, Length=436, Percent_Identity=47.7064220183486, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324974, Length=436, Percent_Identity=47.7064220183486, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324969, Length=436, Percent_Identity=47.7064220183486, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=48.2678983833718, Blast_Score=357, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24580918, Length=439, Percent_Identity=51.4806378132118, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580916, Length=439, Percent_Identity=51.4806378132118, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580920, Length=439, Percent_Identity=51.4806378132118, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580914, Length=439, Percent_Identity=51.4806378132118, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=51.8518518518518, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=51.8518518518518, Blast_Score=392, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_ALISL (B6EKL8)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263862.1
- ProteinModelPortal:   B6EKL8
- GeneID:   6988567
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2514
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45656; Mature: 45525

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 209-209 ACT_SITE 343-343 BINDING 159-159 BINDING 168-168 BINDING 291-291 BINDING 318-318 BINDING 343-343 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIVKVLGREIIDSRGNPTVEAEVHLESGFVGMAAAPSGASTGSREALELRDGDKSRFL
CHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAIAAVNGPIAEALIGKDAKNQAEIDQIMIDLDGTENKAKFGANAILAVSLANAK
HHHHHHHHHHCCCHHHHHHHCCCCCCHHHHEEEEEECCCCCCCHHHCCCEEEEEEECCCH
AAAAAKSMPLYAHIAELNGTPGVFSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLK
HHHHHHCCCCEEEHHHCCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EAVRMGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYELGKD
HHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
VTLAMDCAASEFYNKEDGIYDLKGEGKKFTAEEFNHYLAGLVEQFPIVSIEDGLDESDWA
EEEEEHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCC
GFKHQTELLGDKIQLVGDDLFVTNTKILARGIEEGITNSILIKFNQIGSLTETLAAIKMA
CCCHHHHHHCCCEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEHHHCHHHHHHHHHHHH
KDAGFTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEALGEL
HCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHH
APFNGLKEVKGQ
CCCCCCHHHCCC
>Mature Secondary Structure 
SKIVKVLGREIIDSRGNPTVEAEVHLESGFVGMAAAPSGASTGSREALELRDGDKSRFL
HHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAIAAVNGPIAEALIGKDAKNQAEIDQIMIDLDGTENKAKFGANAILAVSLANAK
HHHHHHHHHHCCCHHHHHHHCCCCCCHHHHEEEEEECCCCCCCHHHCCCEEEEEEECCCH
AAAAAKSMPLYAHIAELNGTPGVFSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLK
HHHHHHCCCCEEEHHHCCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EAVRMGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYELGKD
HHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
VTLAMDCAASEFYNKEDGIYDLKGEGKKFTAEEFNHYLAGLVEQFPIVSIEDGLDESDWA
EEEEEHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCC
GFKHQTELLGDKIQLVGDDLFVTNTKILARGIEEGITNSILIKFNQIGSLTETLAAIKMA
CCCHHHHHHCCCEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEHHHCHHHHHHHHHHHH
KDAGFTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEALGEL
HCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHH
APFNGLKEVKGQ
CCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA