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The map label for this gene is ispD

Identifier: 209695930

GI number: 209695930

Start: 2691154

End: 2691873

Strand: Reverse

Name: ispD

Synonym: VSAL_I2512

Alternate gene names: 209695930

Gene position: 2691873-2691154 (Counterclockwise)

Preceding gene: 209695931

Following gene: 209695929

Centisome position: 80.95

GC content: 41.53

Gene sequence:

>720_bases
ATGTCTTCATTGTCTCCTTTATTTATTACCGTAATCGTGCCTGCTGCAGGTGTTGGCAGCCGAATGAAAGCCGATCGCCC
AAAACAATATTTAGTACTTGATGGTAAAACAGTCATTGAACATACCATTGAGAACTTATTGGCCTTTCCTATGGTCAATA
CTGTCATTGTTGCAATCAGCGATGATGACCCATATTTTGATGCGTTATCTATTGCTACCGATAAGAGAGTTATTCGAGTC
TCTGGTGGGGAAGAGCGTGCTGATTCTGTTCTTTCTGGTCTAGAATACGTACAAAAAAACACATTAAGTCAATGGGTGAT
GGTTCATGATGCGGCACGCCCTTGCCTTCGCCATAGCGACATTGCTAAGTTAATTGAAGCCGTGATCCCGGATCATCTGG
GTGGTATATTAGCCTCGCCAGTTCGAGATACGATGAAGCAGGCAAATTCAGATTTAAGCATCAGTACAACGATTGATCGC
AGTGTGTTGTGGCATGCATTAACACCGCAACTGTTCACTACCGAGTTATTGTATACCGCATTAAAAACAGGTTTAGAACA
GGGCTTATCGATTACGGATGAGTCTTCTGCTATCGAATTTATGGGATATCAGCCTAAACTCATACAAGGTCGGGCCGATA
ACTTAAAAATAACTCAACCTGAAGATCTTGATTTAGCAGAGTTCTACCTTCAAAAAATGAAAAAGGAAATCAACGAATGA

Upstream 100 bases:

>100_bases
TTCAGCGGCAATAGAAGAGCGAGCTAGGCATGAACTCGGTATGATTAAAAAAGGTGAGACCTTTTATCGCATTATTGATG
AAAATAGTGAGGAATAAAAA

Downstream 100 bases:

>100_bases
GAATTGGACACGGATTTGATGTACATAAATTTGGTGGAGAAGGCCCAGTTATTATTGGTGGAGTCAGCGTTCCTTATGAG
CAAGGGTTAATTGCTCATTC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSSLSPLFITVIVPAAGVGSRMKADRPKQYLVLDGKTVIEHTIENLLAFPMVNTVIVAISDDDPYFDALSIATDKRVIRV
SGGEERADSVLSGLEYVQKNTLSQWVMVHDAARPCLRHSDIAKLIEAVIPDHLGGILASPVRDTMKQANSDLSISTTIDR
SVLWHALTPQLFTTELLYTALKTGLEQGLSITDESSAIEFMGYQPKLIQGRADNLKITQPEDLDLAEFYLQKMKKEINE

Sequences:

>Translated_239_residues
MSSLSPLFITVIVPAAGVGSRMKADRPKQYLVLDGKTVIEHTIENLLAFPMVNTVIVAISDDDPYFDALSIATDKRVIRV
SGGEERADSVLSGLEYVQKNTLSQWVMVHDAARPCLRHSDIAKLIEAVIPDHLGGILASPVRDTMKQANSDLSISTTIDR
SVLWHALTPQLFTTELLYTALKTGLEQGLSITDESSAIEFMGYQPKLIQGRADNLKITQPEDLDLAEFYLQKMKKEINE
>Mature_238_residues
SSLSPLFITVIVPAAGVGSRMKADRPKQYLVLDGKTVIEHTIENLLAFPMVNTVIVAISDDDPYFDALSIATDKRVIRVS
GGEERADSVLSGLEYVQKNTLSQWVMVHDAARPCLRHSDIAKLIEAVIPDHLGGILASPVRDTMKQANSDLSISTTIDRS
VLWHALTPQLFTTELLYTALKTGLEQGLSITDESSAIEFMGYQPKLIQGRADNLKITQPEDLDLAEFYLQKMKKEINE

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=243, Percent_Identity=23.0452674897119, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1789104, Length=233, Percent_Identity=50.2145922746781, Blast_Score=243, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_ALISL (B6EKL6)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263860.1
- GeneID:   6988727
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2512
- HOGENOM:   HBG672839
- OMA:   SKVIVVC
- ProtClustDB:   PRK00155
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 26413; Mature: 26281

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLSPLFITVIVPAAGVGSRMKADRPKQYLVLDGKTVIEHTIENLLAFPMVNTVIVAIS
CCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHEEEEEEC
DDDPYFDALSIATDKRVIRVSGGEERADSVLSGLEYVQKNTLSQWVMVHDAARPCLRHSD
CCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKLIEAVIPDHLGGILASPVRDTMKQANSDLSISTTIDRSVLWHALTPQLFTTELLYTA
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCHHHHHHHHHHHH
LKTGLEQGLSITDESSAIEFMGYQPKLIQGRADNLKITQPEDLDLAEFYLQKMKKEINE
HHHHHHHCCCCCCCCCHHHHCCCCCCEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSLSPLFITVIVPAAGVGSRMKADRPKQYLVLDGKTVIEHTIENLLAFPMVNTVIVAIS
CCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHEEEEEEC
DDDPYFDALSIATDKRVIRVSGGEERADSVLSGLEYVQKNTLSQWVMVHDAARPCLRHSD
CCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKLIEAVIPDHLGGILASPVRDTMKQANSDLSISTTIDRSVLWHALTPQLFTTELLYTA
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCHHHHHHHHHHHH
LKTGLEQGLSITDESSAIEFMGYQPKLIQGRADNLKITQPEDLDLAEFYLQKMKKEINE
HHHHHHHCCCCCCCCCHHHHCCCCCCEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA