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The map label for this gene is ispF

Identifier: 209695929

GI number: 209695929

Start: 2690678

End: 2691157

Strand: Reverse

Name: ispF

Synonym: VSAL_I2511

Alternate gene names: 209695929

Gene position: 2691157-2690678 (Counterclockwise)

Preceding gene: 209695930

Following gene: 209695928

Centisome position: 80.93

GC content: 42.92

Gene sequence:

>480_bases
ATGAGAATTGGACACGGATTTGATGTACATAAATTTGGTGGAGAAGGCCCAGTTATTATTGGTGGAGTCAGCGTTCCTTA
TGAGCAAGGGTTAATTGCTCATTCAGATGGTGATGTTGCACTGCATGCATTGTGTGATGCTTTGCTTGGTGCCATTGCGG
AAGGAGATATTGGTCGTCATTTCCCTGATACTGATGATAAATGGAAAGGCGCCGATAGCAGAGCTTTGCTTAGAGATGTA
TATCGTCGAGTTAAAGAAAAAGGCTTAGAGCTAGGGAATGCGGATGTCACAATCATTGCACAAGCCCCTAAGATGGCGCC
TCATATTAAACATATGTGCGCTGCTATTGCTGAAGATTTAGAAACAAGCTTGGATAATGTGAACGTCAAAGCGACCACGT
CCGAGGGTTTAGGCTTTACTGGTAGAAAAGAAGGCATCGCTTGTGAAGCTGTGGTGCTGTTAGTTAAAAAATCTTATTAA

Upstream 100 bases:

>100_bases
AACTCATACAAGGTCGGGCCGATAACTTAAAAATAACTCAACCTGAAGATCTTGATTTAGCAGAGTTCTACCTTCAAAAA
ATGAAAAAGGAAATCAACGA

Downstream 100 bases:

>100_bases
TTAGAGACAAACTTATGTCAGACATCATGTCTACTTTTTCATGGTTATACGGAAAGCCAGTTGCAACTGGTAAATTAAAG
CAGCTACCTGAACACTTTAT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MRIGHGFDVHKFGGEGPVIIGGVSVPYEQGLIAHSDGDVALHALCDALLGAIAEGDIGRHFPDTDDKWKGADSRALLRDV
YRRVKEKGLELGNADVTIIAQAPKMAPHIKHMCAAIAEDLETSLDNVNVKATTSEGLGFTGRKEGIACEAVVLLVKKSY

Sequences:

>Translated_159_residues
MRIGHGFDVHKFGGEGPVIIGGVSVPYEQGLIAHSDGDVALHALCDALLGAIAEGDIGRHFPDTDDKWKGADSRALLRDV
YRRVKEKGLELGNADVTIIAQAPKMAPHIKHMCAAIAEDLETSLDNVNVKATTSEGLGFTGRKEGIACEAVVLLVKKSY
>Mature_159_residues
MRIGHGFDVHKFGGEGPVIIGGVSVPYEQGLIAHSDGDVALHALCDALLGAIAEGDIGRHFPDTDDKWKGADSRALLRDV
YRRVKEKGLELGNADVTIIAQAPKMAPHIKHMCAAIAEDLETSLDNVNVKATTSEGLGFTGRKEGIACEAVVLLVKKSY

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=158, Percent_Identity=73.4177215189873, Blast_Score=212, Evalue=8e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_ALISL (B6EKL5)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263859.1
- GeneID:   6989338
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2511
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16931; Mature: 16931

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGHGFDVHKFGGEGPVIIGGVSVPYEQGLIAHSDGDVALHALCDALLGAIAEGDIGRH
CCCCCCCCHHEECCCCCEEEECCCCCHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCC
FPDTDDKWKGADSRALLRDVYRRVKEKGLELGNADVTIIAQAPKMAPHIKHMCAAIAEDL
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
ETSLDNVNVKATTSEGLGFTGRKEGIACEAVVLLVKKSY
HHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRIGHGFDVHKFGGEGPVIIGGVSVPYEQGLIAHSDGDVALHALCDALLGAIAEGDIGRH
CCCCCCCCHHEECCCCCEEEECCCCCHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCC
FPDTDDKWKGADSRALLRDVYRRVKEKGLELGNADVTIIAQAPKMAPHIKHMCAAIAEDL
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
ETSLDNVNVKATTSEGLGFTGRKEGIACEAVVLLVKKSY
HHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA