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The map label for this gene is truD

Identifier: 209695928

GI number: 209695928

Start: 2689617

End: 2690663

Strand: Reverse

Name: truD

Synonym: VSAL_I2510

Alternate gene names: 209695928

Gene position: 2690663-2689617 (Counterclockwise)

Preceding gene: 209695929

Following gene: 209695927

Centisome position: 80.92

GC content: 39.92

Gene sequence:

>1047_bases
ATGTCAGACATCATGTCTACTTTTTCATGGTTATACGGAAAGCCAGTTGCAACTGGTAAATTAAAGCAGCTACCTGAACA
CTTTATTGTAAAAGAAGTATTAGGGTATGAGTTCACTGGTAAAGGTGAACACTTAATGGTTAAAATTCGTAAAACTGGAG
AAAACACGAAATACGTAGCGAATGAATTCGCTAAGTTTTGTGGTGTTAAATCAAAAGACATCAGCTGGGCTGGTTTGAAA
GATCGCCATGCGGTGACTGAACAGTGGTTAAGCGTTCATCTTCCAAAAAGTGATCACTTGAATTTTGTTCTATTTGAAGT
GGCACACCCTGGTGTTGAAATTTTAGAAATGACGCGTCATCATAAAAAATTACGTCCAGGCGATCTTCTCGGAAATAGCT
TTCAGTTAATTGCGACTGAAGTGACTGATATGGATGATGTACTGGCTCGTTTAGAGAAGGTAAAAGTAACGGGTGTACCA
AACTATTTTGGTGCTCAACGCTTTGGACATGAAGGCAATAACGTAACAGAAGCGCGTCGTTGGGGGCGTGAAAATGTCCG
TACTCGTGATAATACGAAACGCAGTTTTTATCTATCAGCAGCGCGTTCGTGGATTTTTAACCATATTATTTCTCAAAGAA
TAACAGAGGGTTACTTCTCTCTACCTGTTGATGGTGATATTCTACTTGATCAAAACGATCGTACGGTTAATGAGAATGTA
ACGTCAGAAGACTGCATTCAAAAAATTAAAAAGGGTGAATTATCAATCAGTGCAGCACTTGCTGGAGATAATCAACTGCC
AACGACAGAGAAAGCATTAATGTTTGAGCAACCACAACTGGATGCTGAACCAGACTTAATGGCCTTGATCCGTGGTAACC
GTATGCGTCATGAACGCCGTAGCATTGAATTACATCCAGAGAACCTACATTGGTCTGTGGAAGGCGATCAACTTACCTTA
AACTTTTCACTAACATCGGGCAGTTTTGCTACGGTTATCGTTCGTGAATTGTTACAAGAAATTGAAGTTGAACGTACATA
CGATTAG

Upstream 100 bases:

>100_bases
CCACGTCCGAGGGTTTAGGCTTTACTGGTAGAAAAGAAGGCATCGCTTGTGAAGCTGTGGTGCTGTTAGTTAAAAAATCT
TATTAATTAGAGACAAACTT

Downstream 100 bases:

>100_bases
AACGACTTAAGAAATGAAGTGGAGAACTGAGTGAAGAAATTGCATATATTGCTAAGTAATGATGATGGGGTATTTGCTGA
AGGGATGAATACTCTTGCTT

Product: tRNA pseudouridine synthase D

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSDIMSTFSWLYGKPVATGKLKQLPEHFIVKEVLGYEFTGKGEHLMVKIRKTGENTKYVANEFAKFCGVKSKDISWAGLK
DRHAVTEQWLSVHLPKSDHLNFVLFEVAHPGVEILEMTRHHKKLRPGDLLGNSFQLIATEVTDMDDVLARLEKVKVTGVP
NYFGAQRFGHEGNNVTEARRWGRENVRTRDNTKRSFYLSAARSWIFNHIISQRITEGYFSLPVDGDILLDQNDRTVNENV
TSEDCIQKIKKGELSISAALAGDNQLPTTEKALMFEQPQLDAEPDLMALIRGNRMRHERRSIELHPENLHWSVEGDQLTL
NFSLTSGSFATVIVRELLQEIEVERTYD

Sequences:

>Translated_348_residues
MSDIMSTFSWLYGKPVATGKLKQLPEHFIVKEVLGYEFTGKGEHLMVKIRKTGENTKYVANEFAKFCGVKSKDISWAGLK
DRHAVTEQWLSVHLPKSDHLNFVLFEVAHPGVEILEMTRHHKKLRPGDLLGNSFQLIATEVTDMDDVLARLEKVKVTGVP
NYFGAQRFGHEGNNVTEARRWGRENVRTRDNTKRSFYLSAARSWIFNHIISQRITEGYFSLPVDGDILLDQNDRTVNENV
TSEDCIQKIKKGELSISAALAGDNQLPTTEKALMFEQPQLDAEPDLMALIRGNRMRHERRSIELHPENLHWSVEGDQLTL
NFSLTSGSFATVIVRELLQEIEVERTYD
>Mature_347_residues
SDIMSTFSWLYGKPVATGKLKQLPEHFIVKEVLGYEFTGKGEHLMVKIRKTGENTKYVANEFAKFCGVKSKDISWAGLKD
RHAVTEQWLSVHLPKSDHLNFVLFEVAHPGVEILEMTRHHKKLRPGDLLGNSFQLIATEVTDMDDVLARLEKVKVTGVPN
YFGAQRFGHEGNNVTEARRWGRENVRTRDNTKRSFYLSAARSWIFNHIISQRITEGYFSLPVDGDILLDQNDRTVNENVT
SEDCIQKIKKGELSISAALAGDNQLPTTEKALMFEQPQLDAEPDLMALIRGNRMRHERRSIELHPENLHWSVEGDQLTLN
FSLTSGSFATVIVRELLQEIEVERTYD

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Escherichia coli, GI1789102, Length=334, Percent_Identity=43.4131736526946, Blast_Score=271, Evalue=4e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_ALISL (B6EKL4)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263858.1
- ProteinModelPortal:   B6EKL4
- GeneID:   6989270
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2510
- HOGENOM:   HBG541221
- OMA:   ALTRQWF
- ProtClustDB:   PRK00984
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326
- TIGRFAMs:   TIGR00094

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 39725; Mature: 39593

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 81-81

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIMSTFSWLYGKPVATGKLKQLPEHFIVKEVLGYEFTGKGEHLMVKIRKTGENTKYVA
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHH
NEFAKFCGVKSKDISWAGLKDRHAVTEQWLSVHLPKSDHLNFVLFEVAHPGVEILEMTRH
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCCEEEEEECCCCHHHHHHHHH
HKKLRPGDLLGNSFQLIATEVTDMDDVLARLEKVKVTGVPNYFGAQRFGHEGNNVTEARR
HHCCCCCHHCCCCEEEEEEHHCCHHHHHHHHHHEEEECCCCHHHHHHCCCCCCCHHHHHH
WGRENVRTRDNTKRSFYLSAARSWIFNHIISQRITEGYFSLPVDGDILLDQNDRTVNENV
CCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEECCCCCCCCCCC
TSEDCIQKIKKGELSISAALAGDNQLPTTEKALMFEQPQLDAEPDLMALIRGNRMRHERR
CHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHEEECCCCCCCCCHHHHHCCCHHHHHHC
SIELHPENLHWSVEGDQLTLNFSLTSGSFATVIVRELLQEIEVERTYD
CEEECCCCEEEEEECCEEEEEEEECCCCHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure 
SDIMSTFSWLYGKPVATGKLKQLPEHFIVKEVLGYEFTGKGEHLMVKIRKTGENTKYVA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHH
NEFAKFCGVKSKDISWAGLKDRHAVTEQWLSVHLPKSDHLNFVLFEVAHPGVEILEMTRH
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCCEEEEEECCCCHHHHHHHHH
HKKLRPGDLLGNSFQLIATEVTDMDDVLARLEKVKVTGVPNYFGAQRFGHEGNNVTEARR
HHCCCCCHHCCCCEEEEEEHHCCHHHHHHHHHHEEEECCCCHHHHHHCCCCCCCHHHHHH
WGRENVRTRDNTKRSFYLSAARSWIFNHIISQRITEGYFSLPVDGDILLDQNDRTVNENV
CCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEECCCCCCCCCCC
TSEDCIQKIKKGELSISAALAGDNQLPTTEKALMFEQPQLDAEPDLMALIRGNRMRHERR
CHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHEEECCCCCCCCCHHHHHCCCHHHHHHC
SIELHPENLHWSVEGDQLTLNFSLTSGSFATVIVRELLQEIEVERTYD
CEEECCCCEEEEEECCEEEEEEEECCCCHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA