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The map label for this gene is aceA [H]

Identifier: 209695862

GI number: 209695862

Start: 2610139

End: 2611467

Strand: Reverse

Name: aceA [H]

Synonym: VSAL_I2438

Alternate gene names: 209695862

Gene position: 2611467-2610139 (Counterclockwise)

Preceding gene: 209695863

Following gene: 209695860

Centisome position: 78.54

GC content: 43.34

Gene sequence:

>1329_bases
ATGACTACATTTAAACCGCAAACTCGCCAACAGCAAATTGATGCGTTAAAAAAAGATTGGGCTGAAAATCCTCGTTGGAA
TGGGGTAACTCGTCCATACACAGCGGAAGAAGTAATTAACCTTCGTGGATCTTTTGCACCAGAGAATACGATTGCTCAAA
AAGGAGCCGATAAGCTGTGGGATCTCGTTAATGGGTCGTCTAAAAAAGGGTATGTGAATTGCCTCGGTGCGTTAACTGGT
GGACAAGCGGTCCAACAAGCGAAAGCTGGGATTGAAGCAATTTATCTTTCTGGTTGGCAGGTGGCAGCAGATAATAATAC
GGCATCAACGATGTACCCTGATCAATCGTTGTATCCAGTAGATTCAGTACCTTCGGTAGTTAAGCGTATTAATAATTCTT
TTCGTCGAGCGGATCAAATTCAGTGGTCAACAGGCACAACACCAGAAGAAGGCATTGATTATTTCCTTCCCATTGTTGCC
GATGCAGAAGCGGGTTTTGGAGGCATTCTAAATGGCTATGAATTGATGCGTAATATGATTGAATCTGGTGCGGCAGGCGT
TCACTTTGAAGATCAGTTAGCGTCGGTGAAAAAATGTGGTCATATGGGGGGCAAAGTATTGGTTCCAACTCAAGAGGCGG
TTCAAAAATTAGTGGCGGCTCGATTGGCGGCTGATGTTGCAGGTACAACAACGTTAGTTATCGCAAGAACCGATGCAAAT
GCCGCCGATTTGTTAACCTCAGATTGCGATGCTTATGATCAAGAGTTTATTGAAGGTGAACGTACTGCTGAAGGGTTCTA
TAAAGTAACGGCAGGGATTGATCAGGCAATTTCTCGTGGTCTTGCTTATTCTCCTTATGCTGATTTAGTGTGGTGTGAAA
CAGCAACGCCATGTTTAGAAGAAGCGCGTAAATTTGCTGAAGCGATTCATGCCGAATATCCAGACCAATTATTAGCGTAT
AACTGTTCGCCATCGTTTAACTGGGAGAAAAATCTTGATTCAGAAACGATTGCGAAGTTCCAACAAGAACTGGCTGATAT
GGGATACAAATACCAATTCATAACACTTGCTGGCATTCATAGCATGTGGTTCAACATGTTTGAATTAGCGCATTCTTATG
CTCAGGGTGAAGGCATGCGTCATTATGTTGAAATGGTTCAGCGTAAAGAATTTGAATCTGCGTCTAAGGGCTATACGTTT
GTCGCGCATCAGCAAGAAGTAGGTACTGGGTACTTCGATAAGATGACAAACACGATTCAAGGCGGTAATTCATCAGTGAC
CGCATTAACGGGCTCAACGGAAGAAGAGCAGTTTAAAACGAATGCCTAA

Upstream 100 bases:

>100_bases
TTCAAGCAGCAAAACTGATGGAGCAATTAACCACCAGCGATCAACTAACGGACTTTTTAACACTCACTGGCTATGACTAT
TTATAAAAGAGGGAACTATT

Downstream 100 bases:

>100_bases
ACCTAGATAGAACCCCATAACATTTTTTGCGGTATACATAATAAGAGATAAGCGTATTACCACGTTTGAGGGAGAATGGA
GTCTCCCTTATCTTTTTTAT

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTTFKPQTRQQQIDALKKDWAENPRWNGVTRPYTAEEVINLRGSFAPENTIAQKGADKLWDLVNGSSKKGYVNCLGALTG
GQAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPVDSVPSVVKRINNSFRRADQIQWSTGTTPEEGIDYFLPIVA
DAEAGFGGILNGYELMRNMIESGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTTTLVIARTDAN
AADLLTSDCDAYDQEFIEGERTAEGFYKVTAGIDQAISRGLAYSPYADLVWCETATPCLEEARKFAEAIHAEYPDQLLAY
NCSPSFNWEKNLDSETIAKFQQELADMGYKYQFITLAGIHSMWFNMFELAHSYAQGEGMRHYVEMVQRKEFESASKGYTF
VAHQQEVGTGYFDKMTNTIQGGNSSVTALTGSTEEEQFKTNA

Sequences:

>Translated_442_residues
MTTFKPQTRQQQIDALKKDWAENPRWNGVTRPYTAEEVINLRGSFAPENTIAQKGADKLWDLVNGSSKKGYVNCLGALTG
GQAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPVDSVPSVVKRINNSFRRADQIQWSTGTTPEEGIDYFLPIVA
DAEAGFGGILNGYELMRNMIESGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTTTLVIARTDAN
AADLLTSDCDAYDQEFIEGERTAEGFYKVTAGIDQAISRGLAYSPYADLVWCETATPCLEEARKFAEAIHAEYPDQLLAY
NCSPSFNWEKNLDSETIAKFQQELADMGYKYQFITLAGIHSMWFNMFELAHSYAQGEGMRHYVEMVQRKEFESASKGYTF
VAHQQEVGTGYFDKMTNTIQGGNSSVTALTGSTEEEQFKTNA
>Mature_441_residues
TTFKPQTRQQQIDALKKDWAENPRWNGVTRPYTAEEVINLRGSFAPENTIAQKGADKLWDLVNGSSKKGYVNCLGALTGG
QAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPVDSVPSVVKRINNSFRRADQIQWSTGTTPEEGIDYFLPIVAD
AEAGFGGILNGYELMRNMIESGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTTTLVIARTDANA
ADLLTSDCDAYDQEFIEGERTAEGFYKVTAGIDQAISRGLAYSPYADLVWCETATPCLEEARKFAEAIHAEYPDQLLAYN
CSPSFNWEKNLDSETIAKFQQELADMGYKYQFITLAGIHSMWFNMFELAHSYAQGEGMRHYVEMVQRKEFESASKGYTFV
AHQQEVGTGYFDKMTNTIQGGNSSVTALTGSTEEEQFKTNA

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=434, Percent_Identity=75.8064516129032, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=267, Percent_Identity=28.0898876404494, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17561814, Length=420, Percent_Identity=55, Blast_Score=469, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71982926, Length=420, Percent_Identity=55, Blast_Score=469, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6320908, Length=290, Percent_Identity=36.2068965517241, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6325263, Length=251, Percent_Identity=36.6533864541833, Blast_Score=161, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 48623; Mature: 48491

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFKPQTRQQQIDALKKDWAENPRWNGVTRPYTAEEVINLRGSFAPENTIAQKGADKLW
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
DLVNGSSKKGYVNCLGALTGGQAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPV
HHHCCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCC
DSVPSVVKRINNSFRRADQIQWSTGTTPEEGIDYFLPIVADAEAGFGGILNGYELMRNMI
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHEEECCCCCCCHHHHHHHHHHHHH
ESGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTTTLVIARTDAN
HCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
AADLLTSDCDAYDQEFIEGERTAEGFYKVTAGIDQAISRGLAYSPYADLVWCETATPCLE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHH
EARKFAEAIHAEYPDQLLAYNCSPSFNWEKNLDSETIAKFQQELADMGYKYQFITLAGIH
HHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHH
SMWFNMFELAHSYAQGEGMRHYVEMVQRKEFESASKGYTFVAHQQEVGTGYFDKMTNTIQ
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHHHHHHC
GGNSSVTALTGSTEEEQFKTNA
CCCCEEEEEECCCCHHHHCCCC
>Mature Secondary Structure 
TTFKPQTRQQQIDALKKDWAENPRWNGVTRPYTAEEVINLRGSFAPENTIAQKGADKLW
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
DLVNGSSKKGYVNCLGALTGGQAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPV
HHHCCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCC
DSVPSVVKRINNSFRRADQIQWSTGTTPEEGIDYFLPIVADAEAGFGGILNGYELMRNMI
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHEEECCCCCCCHHHHHHHHHHHHH
ESGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTTTLVIARTDAN
HCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
AADLLTSDCDAYDQEFIEGERTAEGFYKVTAGIDQAISRGLAYSPYADLVWCETATPCLE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHH
EARKFAEAIHAEYPDQLLAYNCSPSFNWEKNLDSETIAKFQQELADMGYKYQFITLAGIH
HHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHH
SMWFNMFELAHSYAQGEGMRHYVEMVQRKEFESASKGYTFVAHQQEVGTGYFDKMTNTIQ
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHHHHHHC
GGNSSVTALTGSTEEEQFKTNA
CCCCEEEEEECCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]