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The map label for this gene is hns [H]

Identifier: 209695586

GI number: 209695586

Start: 2302858

End: 2303271

Strand: Direct

Name: hns [H]

Synonym: VSAL_I2145

Alternate gene names: 209695586

Gene position: 2302858-2303271 (Clockwise)

Preceding gene: 209695576

Following gene: 209695589

Centisome position: 69.26

GC content: 42.51

Gene sequence:

>414_bases
ATGTCTGATACTATTAAAACATTACTAAACCTACGTAGCCTACGTAGCCTACGCGCGTTATCTCGTGAAATGACATTAGA
GCAACTAGAAGAAGCGCTTGAAAAGCTTCAAGCAGTTGTTGCTGAACGTCAAGAAACAGAAGCTGAAGATCGCGCTCTAC
TTGCTGAAAAACAAGCGAAGCTTGAAGAATTCCGTCAAATGATGATTGAGAGCGGTATCGCTCCTGAAGATCTACTAGAC
ACAATGTCTTCTACTACAGTTAAAACAAAGCAGAAACGTGCTCCACGTCCAGCTAAGTACAAATTCTTAGACCACGCTGG
CGAAGAAAAAACATGGACAGGCCAAGGCCGTACTCCATCAGCACTTCAAGTACACCTTGATTCTGGTAAATCATTAGAAG
AGTTCGCTATCTAA

Upstream 100 bases:

>100_bases
TTATTATCATTAATAATTAATTATTTCCTTATCCCATAGTTTGATATATTATTTAATGGTTATATAAAAATTACATTATC
AAATTAATATGGAATCAAAC

Downstream 100 bases:

>100_bases
ATTGTTCTTATAGAACAATGCGATTTCGATAAAAAGCCATTCTTTTGAATGGCTTTTTTTATTCTAAATATCTATTATTT
AGAATACGTATGCTATACCA

Product: DNA binding protein, global metabolism regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MSDTIKTLLNLRSLRSLRALSREMTLEQLEEALEKLQAVVAERQETEAEDRALLAEKQAKLEEFRQMMIESGIAPEDLLD
TMSSTTVKTKQKRAPRPAKYKFLDHAGEEKTWTGQGRTPSALQVHLDSGKSLEEFAI

Sequences:

>Translated_137_residues
MSDTIKTLLNLRSLRSLRALSREMTLEQLEEALEKLQAVVAERQETEAEDRALLAEKQAKLEEFRQMMIESGIAPEDLLD
TMSSTTVKTKQKRAPRPAKYKFLDHAGEEKTWTGQGRTPSALQVHLDSGKSLEEFAI
>Mature_136_residues
SDTIKTLLNLRSLRSLRALSREMTLEQLEEALEKLQAVVAERQETEAEDRALLAEKQAKLEEFRQMMIESGIAPEDLLDT
MSSTTVKTKQKRAPRPAKYKFLDHAGEEKTWTGQGRTPSALQVHLDSGKSLEEFAI

Specific function: Binds tightly to ds-DNA, increases its thermal stability and inhibits transcription [H]

COG id: COG2916

COG function: function code R; DNA-binding protein H-NS

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the histone-like protein H-NS family [H]

Homologues:

Organism=Escherichia coli, GI1787489, Length=138, Percent_Identity=50, Blast_Score=120, Evalue=3e-29,
Organism=Escherichia coli, GI1789023, Length=130, Percent_Identity=47.6923076923077, Blast_Score=114, Evalue=2e-27,

Paralogues:

None

Copy number: 820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1780 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 1020 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 4632 Molecules/Cell In: Gr

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001801 [H]

Pfam domain/function: PF00816 Histone_HNS [H]

EC number: NA

Molecular weight: Translated: 15516; Mature: 15385

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTIKTLLNLRSLRSLRALSREMTLEQLEEALEKLQAVVAERQETEAEDRALLAEKQAK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEFRQMMIESGIAPEDLLDTMSSTTVKTKQKRAPRPAKYKFLDHAGEEKTWTGQGRTPS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCC
ALQVHLDSGKSLEEFAI
CEEEECCCCCCHHHHCC
>Mature Secondary Structure 
SDTIKTLLNLRSLRSLRALSREMTLEQLEEALEKLQAVVAERQETEAEDRALLAEKQAK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEFRQMMIESGIAPEDLLDTMSSTTVKTKQKRAPRPAKYKFLDHAGEEKTWTGQGRTPS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCC
ALQVHLDSGKSLEEFAI
CEEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]