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The map label for this gene is bcr [H]

Identifier: 209695470

GI number: 209695470

Start: 2161267

End: 2162472

Strand: Direct

Name: bcr [H]

Synonym: VSAL_I2019

Alternate gene names: 209695470

Gene position: 2161267-2162472 (Clockwise)

Preceding gene: 209695469

Following gene: 209695471

Centisome position: 65.0

GC content: 42.45

Gene sequence:

>1206_bases
ATGTCATCCTCAGCTAGCGATCTTTCTGAAGCCCCTAAGTTAAGTCTTTTATTAGTCATCATTTTGGGTGCTATTTCAGC
GCTAACACCACTTGCTATTGATATGTATGTTCCTGCAATGCCGAGCATTGCGGCGGATCTTGGTGTATCTGCGGGTTCAG
TTCAGATGACATTAACAGCTTATACGGCCGGCTTTGCGATTGCACAACTGATTCATGGGCCACTGGCTGACAGTTATGGT
CGCCGTCCGGTATTAATTATTGGTACGGTGCTCTTTGCCATTTGTGCGGTGATTGGTGCACAGGTTGATGGTATTGAATC
ATTAATGTATATCCGTGTTTTACAAGGGATTGCAGGAGCGGCATCAGCGGTGGTTATTCAAGCTATTGTTCGTGACATGT
TTAATAAAGAAGATTTTGCCCGCATGATGTCTTTCATCACGTTGGTCATGACAATTGCCCCACTGGTCGCGCCGATGATT
GGTGGACACATGGCGGTATGGTTCGGTTGGCGTTCAATTTTTTGGTTACTGGCTATTTTTGCCGTGTTAACTATTTTTGC
AATTCTTTGGCGGATCCCTGAAACGTTGGCAGAAGAGAATCGCTCTCCTTTTAGATTAATGACGTCGTTTAGAAATTACC
GTTCATTATTTAAAAACCCAGTGTCACTTGGTTTGATTTTCTCTGGTGCTTTTTCTTTTGCTGGGATGTTTTCATTTTTA
ACGGCAGGCTCATTTGTTTACATCGATATTTATGGTGTGAGCGTGTCTAATTTTGGTTATTTATTTGGTTTAAACGTTGT
TTTCTTAATTGTTATGACCGCGATTAACGGTCGTATGGTTAAAAAAATGGGTTCCCATAATATGATTAAGTTCGGCCTTT
CTATTCAACTGTTTGCTGGCATTCTGATGATGGTCGGGCAACTATTAAACTGGGGTCTATGGGGAACGGTTATTCCTGTT
GTATTGTTTGTGGGTTGTATCTCGACGATTGGCAGTAATACGATGGGCTTATTGTTAAGCCACTACCCTAAAATGGCGGG
AACCGCATCATCATTAGCGGGCACATTGAGATTTGGTACGGGGTCACTTGTTGGCGCTTGTATTGCAATGTTTCCTGCTG
ATTCTGCTTGGCCTATGGTGAGTTCCATGGCAGCATGTTCTATCTTATCTTTTGGTTGTTATTTTGTTTTTGGACGTAAA
GCGTAA

Upstream 100 bases:

>100_bases
ATTCTTGCAAAGAGAACGAATATAAAAAGCATGTTGTAATCGGGCGTGATAACATGAAAAATCCTTTCTTGTTCCCTATT
TTTTAGTTGGTCTAAACCCT

Downstream 100 bases:

>100_bases
ATGAGTAATGAGACAAACACAGTGCACTATTATCAAGAAATTCAAAAAGTAATTAATGAAGCTCTTACTGACTTACAAGA
AAGTAAGGACAGTGGACGCT

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSSSASDLSEAPKLSLLLVIILGAISALTPLAIDMYVPAMPSIAADLGVSAGSVQMTLTAYTAGFAIAQLIHGPLADSYG
RRPVLIIGTVLFAICAVIGAQVDGIESLMYIRVLQGIAGAASAVVIQAIVRDMFNKEDFARMMSFITLVMTIAPLVAPMI
GGHMAVWFGWRSIFWLLAIFAVLTIFAILWRIPETLAEENRSPFRLMTSFRNYRSLFKNPVSLGLIFSGAFSFAGMFSFL
TAGSFVYIDIYGVSVSNFGYLFGLNVVFLIVMTAINGRMVKKMGSHNMIKFGLSIQLFAGILMMVGQLLNWGLWGTVIPV
VLFVGCISTIGSNTMGLLLSHYPKMAGTASSLAGTLRFGTGSLVGACIAMFPADSAWPMVSSMAACSILSFGCYFVFGRK
A

Sequences:

>Translated_401_residues
MSSSASDLSEAPKLSLLLVIILGAISALTPLAIDMYVPAMPSIAADLGVSAGSVQMTLTAYTAGFAIAQLIHGPLADSYG
RRPVLIIGTVLFAICAVIGAQVDGIESLMYIRVLQGIAGAASAVVIQAIVRDMFNKEDFARMMSFITLVMTIAPLVAPMI
GGHMAVWFGWRSIFWLLAIFAVLTIFAILWRIPETLAEENRSPFRLMTSFRNYRSLFKNPVSLGLIFSGAFSFAGMFSFL
TAGSFVYIDIYGVSVSNFGYLFGLNVVFLIVMTAINGRMVKKMGSHNMIKFGLSIQLFAGILMMVGQLLNWGLWGTVIPV
VLFVGCISTIGSNTMGLLLSHYPKMAGTASSLAGTLRFGTGSLVGACIAMFPADSAWPMVSSMAACSILSFGCYFVFGRK
A
>Mature_400_residues
SSSASDLSEAPKLSLLLVIILGAISALTPLAIDMYVPAMPSIAADLGVSAGSVQMTLTAYTAGFAIAQLIHGPLADSYGR
RPVLIIGTVLFAICAVIGAQVDGIESLMYIRVLQGIAGAASAVVIQAIVRDMFNKEDFARMMSFITLVMTIAPLVAPMIG
GHMAVWFGWRSIFWLLAIFAVLTIFAILWRIPETLAEENRSPFRLMTSFRNYRSLFKNPVSLGLIFSGAFSFAGMFSFLT
AGSFVYIDIYGVSVSNFGYLFGLNVVFLIVMTAINGRMVKKMGSHNMIKFGLSIQLFAGILMMVGQLLNWGLWGTVIPVV
LFVGCISTIGSNTMGLLLSHYPKMAGTASSLAGTLRFGTGSLVGACIAMFPADSAWPMVSSMAACSILSFGCYFVFGRKA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=373, Percent_Identity=50.6702412868633, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI48994889, Length=348, Percent_Identity=29.3103448275862, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87082312, Length=350, Percent_Identity=25.4285714285714, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790146, Length=242, Percent_Identity=28.9256198347107, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790794, Length=288, Percent_Identity=26.3888888888889, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787065, Length=186, Percent_Identity=29.5698924731183, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI2367379, Length=206, Percent_Identity=27.6699029126214, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081738, Length=175, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787880, Length=286, Percent_Identity=24.1258741258741, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790195, Length=136, Percent_Identity=27.2058823529412, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319480, Length=274, Percent_Identity=24.0875912408759, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=215, Percent_Identity=24.1860465116279, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319517, Length=189, Percent_Identity=24.8677248677249, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42899; Mature: 42768

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure 
SSSASDLSEAPKLSLLLVIILGAISALTPLAIDMYVPAMPSIAADLGVSAGSVQMTLTA
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEHHH
YTAGFAIAQLIHGPLADSYGRRPVLIIGTVLFAICAVIGAQVDGIESLMYIRVLQGIAGA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ASAVVIQAIVRDMFNKEDFARMMSFITLVMTIAPLVAPMIGGHMAVWFGWRSIFWLLAIF
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AVLTIFAILWRIPETLAEENRSPFRLMTSFRNYRSLFKNPVSLGLIFSGAFSFAGMFSFL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TAGSFVYIDIYGVSVSNFGYLFGLNVVFLIVMTAINGRMVKKMGSHNMIKFGLSIQLFAG
HCCCEEEEEEEEECHHCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECHHHHHHHH
ILMMVGQLLNWGLWGTVIPVVLFVGCISTIGSNTMGLLLSHYPKMAGTASSLAGTLRFGT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHCCCHHHHHHHHHCCC
GSLVGACIAMFPADSAWPMVSSMAACSILSFGCYFVFGRKA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]