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The map label for this gene is fdnI [H]

Identifier: 209694972

GI number: 209694972

Start: 1573822

End: 1574841

Strand: Direct

Name: fdnI [H]

Synonym: VSAL_I1455

Alternate gene names: 209694972

Gene position: 1573822-1574841 (Clockwise)

Preceding gene: 209694971

Following gene: 209694973

Centisome position: 47.33

GC content: 43.04

Gene sequence:

>1020_bases
ATGTTGACACAATTAAAACGTCTTTCTCTTTCGTTATTGCTGCCGTTATTGGCAGCATTAACGCTTTCATTTTCTGTGCC
TAGCATGGCAAATGAAGGCACGACGAATAAGAGTGCAGAGAAAGAAATGACCCAACTTGGTGGCGCTGATTTTTGGCGTC
AAGTAAGAGCGGGCGAAGAAGGCTATACAACCTCACAGTCACCAGAGCATGGTGTACTTATCAGTACTGCTGGCGAAACA
TGGTATGTATTGAAAGAGAAGTGGATGTCACCATTAGGTGCATTAGCTATCTTTGGTAGTTTAGCGATGGTGGCACTGGC
GTACTTTACGATTGGTCCCCTAAAGTTAAGCAAACCAAAAACAGGACGTCGAATTCAGCGTTGGAGCAAAATGGACCGTG
CATTGCACTGGAGCATGGCGTTCACGTTTTTAACGCTCGCGTTCAGTGGTTTATGTTTGGTCTATGGTAAACACTTTTTA
AAGCCAGTATTACCGACTGATATTTGGGGCATGATCATTTATGCCGCGAAGCAGTACCACAACTACATGGGTCCAATTTT
TGGCATCCTATTAATGACTGTTTTAGTGAAATGGTGGAGAAAATCCATTGTAAACAAGACAGACATTCAATGGTTCATGA
AAATGGGCGGCATGGTTGGTAAGCATAAAGGCTCGCATCCATCAGCAGGTTTCTCAAACGGTGGTGAAAAAGCGATCTTC
TGGCTTCTTATCTGGTTTGGTGCTATCGCAGCGATCAGTGGTTTTGTATTAGATTTCCCAATTTTCGGTCAATTACGCCG
TGATATGGAATTGTCTAACTTGATCCATATGTTTGCAGCATTGATCTTGATTTGTGGTTTCATCTTCCACATCTATATCG
GTCTATTCGGTATGGAAGCGGGACTAGAAGGCATGGTGACAGGCGATGTTGACGAAACATGGGCTAAAGAACACCACGAT
TTATGGTATGAAGAAGTAAAAGACCTACCAGAGAATCAGCCAAAAGACAAAAACAGCTAA

Upstream 100 bases:

>100_bases
CGCCAACGTGTTGTAGAACGTGGTGCGAAAGATGCAGGTTGGACAAATGGTGAAGACTTATCGTTTGATGCGACTAAGAG
CTAATCCAGTGGAGAGAGTG

Downstream 100 bases:

>100_bases
TAGCGAGTTAATATTCGTATAGCGAAAATTGGTAAAACCAAAAGCCGAGCAGGGTAACTTGCTCGGCTTTTTTGTATCTA
GTGATTGGGAAAAAGAGAAA

Product: formate dehydrogenase

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MLTQLKRLSLSLLLPLLAALTLSFSVPSMANEGTTNKSAEKEMTQLGGADFWRQVRAGEEGYTTSQSPEHGVLISTAGET
WYVLKEKWMSPLGALAIFGSLAMVALAYFTIGPLKLSKPKTGRRIQRWSKMDRALHWSMAFTFLTLAFSGLCLVYGKHFL
KPVLPTDIWGMIIYAAKQYHNYMGPIFGILLMTVLVKWWRKSIVNKTDIQWFMKMGGMVGKHKGSHPSAGFSNGGEKAIF
WLLIWFGAIAAISGFVLDFPIFGQLRRDMELSNLIHMFAALILICGFIFHIYIGLFGMEAGLEGMVTGDVDETWAKEHHD
LWYEEVKDLPENQPKDKNS

Sequences:

>Translated_339_residues
MLTQLKRLSLSLLLPLLAALTLSFSVPSMANEGTTNKSAEKEMTQLGGADFWRQVRAGEEGYTTSQSPEHGVLISTAGET
WYVLKEKWMSPLGALAIFGSLAMVALAYFTIGPLKLSKPKTGRRIQRWSKMDRALHWSMAFTFLTLAFSGLCLVYGKHFL
KPVLPTDIWGMIIYAAKQYHNYMGPIFGILLMTVLVKWWRKSIVNKTDIQWFMKMGGMVGKHKGSHPSAGFSNGGEKAIF
WLLIWFGAIAAISGFVLDFPIFGQLRRDMELSNLIHMFAALILICGFIFHIYIGLFGMEAGLEGMVTGDVDETWAKEHHD
LWYEEVKDLPENQPKDKNS
>Mature_339_residues
MLTQLKRLSLSLLLPLLAALTLSFSVPSMANEGTTNKSAEKEMTQLGGADFWRQVRAGEEGYTTSQSPEHGVLISTAGET
WYVLKEKWMSPLGALAIFGSLAMVALAYFTIGPLKLSKPKTGRRIQRWSKMDRALHWSMAFTFLTLAFSGLCLVYGKHFL
KPVLPTDIWGMIIYAAKQYHNYMGPIFGILLMTVLVKWWRKSIVNKTDIQWFMKMGGMVGKHKGSHPSAGFSNGGEKAIF
WLLIWFGAIAAISGFVLDFPIFGQLRRDMELSNLIHMFAALILICGFIFHIYIGLFGMEAGLEGMVTGDVDETWAKEHHD
LWYEEVKDLPENQPKDKNS

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=217, Percent_Identity=28.110599078341, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1787750, Length=222, Percent_Identity=27.027027027027, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 38097; Mature: 38097

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQLKRLSLSLLLPLLAALTLSFSVPSMANEGTTNKSAEKEMTQLGGADFWRQVRAGEE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCC
GYTTSQSPEHGVLISTAGETWYVLKEKWMSPLGALAIFGSLAMVALAYFTIGPLKLSKPK
CCCCCCCCCCCEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TGRRIQRWSKMDRALHWSMAFTFLTLAFSGLCLVYGKHFLKPVLPTDIWGMIIYAAKQYH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NYMGPIFGILLMTVLVKWWRKSIVNKTDIQWFMKMGGMVGKHKGSHPSAGFSNGGEKAIF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCHHHHH
WLLIWFGAIAAISGFVLDFPIFGQLRRDMELSNLIHMFAALILICGFIFHIYIGLFGMEA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLEGMVTGDVDETWAKEHHDLWYEEVKDLPENQPKDKNS
CCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLTQLKRLSLSLLLPLLAALTLSFSVPSMANEGTTNKSAEKEMTQLGGADFWRQVRAGEE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCC
GYTTSQSPEHGVLISTAGETWYVLKEKWMSPLGALAIFGSLAMVALAYFTIGPLKLSKPK
CCCCCCCCCCCEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TGRRIQRWSKMDRALHWSMAFTFLTLAFSGLCLVYGKHFLKPVLPTDIWGMIIYAAKQYH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NYMGPIFGILLMTVLVKWWRKSIVNKTDIQWFMKMGGMVGKHKGSHPSAGFSNGGEKAIF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCHHHHH
WLLIWFGAIAAISGFVLDFPIFGQLRRDMELSNLIHMFAALILICGFIFHIYIGLFGMEA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLEGMVTGDVDETWAKEHHDLWYEEVKDLPENQPKDKNS
CCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]