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The map label for this gene is asnB [H]

Identifier: 209694406

GI number: 209694406

Start: 919744

End: 921411

Strand: Reverse

Name: asnB [H]

Synonym: VSAL_I0827

Alternate gene names: 209694406

Gene position: 921411-919744 (Counterclockwise)

Preceding gene: 209694407

Following gene: 209694404

Centisome position: 27.71

GC content: 38.55

Gene sequence:

>1668_bases
ATGTGCTCAATTTTTGGAATTTTAGATATAAAAAGTGACCCTACGCCACTTCGCAAAGTTGCGCTTGAGATGTCAAAAAA
ACTACGTCACAGAGGACCGGATTGGTCTGGAATTTATTCATCAGATAAAGCAATTTTAGCACATGAACGTCTTGCTATTG
TCGGTTTGAACAGTGGTGCTCAACCTTTATACAGCAAAGATAAGCAACACATCCTCGCTGTAAATGGTGAAATCTATAAC
CATAAAGAGCTTCGTGCAAAATACGCCGATGATTATGATTTCCAAACAGATTCTGACTGTGAAGTTATTTTAGCTCTGTA
TCGTGATAAAGGCGCTGATTTATTAGAAGACCTAAACGGAATTTTTGCTTTTATTCTTTATGATGAAGAAAAAGACGAAT
ACTTAATTGGTCGTGACCACATTGGTATCATTCCTATGTATCACGGCTATGATGAGCATGGTAACTATTATATTGCTTCT
GAAATGAAAGCGCTTGTTCCTGTATGTAAATCAATGAGTGAATTTCCTCCGGGTCACTTCTATTCTTCAAAAGATGCGGA
ACCAACTCGTTATTATGTACGAGATTGGATGGAATATGATGCAGTAAAAGATAACCCAACTAGCAAAGACGATCTTAAAC
AAGCATTAGAAGATGCCGTTAAACGTCAATTAATGACAGATGTACCTTATGGTGTCCTTCTATCTGGTGGTCTTGATTCA
TCAATTACTTCAGCGGTTGCAAAGAAATTCGCAGCTATGCGTATTGAAGACAACGAAAAATCTGAAGCATGGTGGCCGCA
ACTGCACTCATTCGCCGTTGGCTTAGAAGGTGCACCTGATTTAAAAGCAGCTCGTGAAGTTGCTAACCAAATAGGTACTG
TCCATCATGAAATGACTTATACCATTCAAGAAGGCCTTGACGCTATTCGTGATGTTATTTATCACATTGAAACATATGAT
GTAACAACAATTCGAGCTTCTACGCCAATGTATTTATTGGGTCGTAAAATCAAAGCAATGGGCATCAAAATGGTACTTTC
AGGTGAAGGTGCTGATGAAATCTTTGGTGGTTACTTATATTTCCATAAAGCACCAAATAAAAAAGAATTCCATGAAGAAA
CAGTACGTAAGCTGCTTGCTTTAAATATGTTTGATTGTGCCCGTGCCAATAAATCACTCGCCGCTTGGGGAGTTGAAGGC
CGCGTTCCTTTCTTAGACAAAGAATTTATTGATGTTGCTATGAGATTGAATCCACAAGATAAGATGTGTGGTAACGGAAA
AATGGAAAAACACATTCTTCGTGAATGTTTTGAAGATGACTTACCAGAAGCCATTGCTTGGAGACAAAAAGAACAATTCT
CTGATGGCGTAGGTTATGGTTGGATTGATTCTTTACGTGAAACTGCTGAAGTAAAAGTAACAGATCAACAAATGGAATCA
GCAAAATTTCGCTTTCCATACAATACTCCATTAACCAAAGAAGCTTATGTATATCGTGAGATCTTTGAAGAACTGTTTCC
TCTTGAAGATGCAGCTAAATGCGTACCTGGCGGACCTTCTATCGCTTGTTCTTCTGCAAAGGCAATTGAGTGGGATGAAT
CATTTAAAAACTGTGCAGACCCATCAGGACGAGCCGTTCAAGCCATACATAACGAAGCTTATAGCTAA

Upstream 100 bases:

>100_bases
ATTTTATTTGTATTTCTGGGTTGTTTTTTTTAGTTATTATGTCAAATTGTATTCAAGACAATAATTTACGATAAAAATTA
AGAAAAAAAGAGGGAATTTT

Downstream 100 bases:

>100_bases
CTAAAATAAGCATTCATAAAAAAAGCGCTCTTAGCGCTTTTTTTATTTAGAAACCCACATTAATATTTTCTATTTTTATT
TCTACTTTTTTATTTATTAA

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MCSIFGILDIKSDPTPLRKVALEMSKKLRHRGPDWSGIYSSDKAILAHERLAIVGLNSGAQPLYSKDKQHILAVNGEIYN
HKELRAKYADDYDFQTDSDCEVILALYRDKGADLLEDLNGIFAFILYDEEKDEYLIGRDHIGIIPMYHGYDEHGNYYIAS
EMKALVPVCKSMSEFPPGHFYSSKDAEPTRYYVRDWMEYDAVKDNPTSKDDLKQALEDAVKRQLMTDVPYGVLLSGGLDS
SITSAVAKKFAAMRIEDNEKSEAWWPQLHSFAVGLEGAPDLKAAREVANQIGTVHHEMTYTIQEGLDAIRDVIYHIETYD
VTTIRASTPMYLLGRKIKAMGIKMVLSGEGADEIFGGYLYFHKAPNKKEFHEETVRKLLALNMFDCARANKSLAAWGVEG
RVPFLDKEFIDVAMRLNPQDKMCGNGKMEKHILRECFEDDLPEAIAWRQKEQFSDGVGYGWIDSLRETAEVKVTDQQMES
AKFRFPYNTPLTKEAYVYREIFEELFPLEDAAKCVPGGPSIACSSAKAIEWDESFKNCADPSGRAVQAIHNEAYS

Sequences:

>Translated_555_residues
MCSIFGILDIKSDPTPLRKVALEMSKKLRHRGPDWSGIYSSDKAILAHERLAIVGLNSGAQPLYSKDKQHILAVNGEIYN
HKELRAKYADDYDFQTDSDCEVILALYRDKGADLLEDLNGIFAFILYDEEKDEYLIGRDHIGIIPMYHGYDEHGNYYIAS
EMKALVPVCKSMSEFPPGHFYSSKDAEPTRYYVRDWMEYDAVKDNPTSKDDLKQALEDAVKRQLMTDVPYGVLLSGGLDS
SITSAVAKKFAAMRIEDNEKSEAWWPQLHSFAVGLEGAPDLKAAREVANQIGTVHHEMTYTIQEGLDAIRDVIYHIETYD
VTTIRASTPMYLLGRKIKAMGIKMVLSGEGADEIFGGYLYFHKAPNKKEFHEETVRKLLALNMFDCARANKSLAAWGVEG
RVPFLDKEFIDVAMRLNPQDKMCGNGKMEKHILRECFEDDLPEAIAWRQKEQFSDGVGYGWIDSLRETAEVKVTDQQMES
AKFRFPYNTPLTKEAYVYREIFEELFPLEDAAKCVPGGPSIACSSAKAIEWDESFKNCADPSGRAVQAIHNEAYS
>Mature_555_residues
MCSIFGILDIKSDPTPLRKVALEMSKKLRHRGPDWSGIYSSDKAILAHERLAIVGLNSGAQPLYSKDKQHILAVNGEIYN
HKELRAKYADDYDFQTDSDCEVILALYRDKGADLLEDLNGIFAFILYDEEKDEYLIGRDHIGIIPMYHGYDEHGNYYIAS
EMKALVPVCKSMSEFPPGHFYSSKDAEPTRYYVRDWMEYDAVKDNPTSKDDLKQALEDAVKRQLMTDVPYGVLLSGGLDS
SITSAVAKKFAAMRIEDNEKSEAWWPQLHSFAVGLEGAPDLKAAREVANQIGTVHHEMTYTIQEGLDAIRDVIYHIETYD
VTTIRASTPMYLLGRKIKAMGIKMVLSGEGADEIFGGYLYFHKAPNKKEFHEETVRKLLALNMFDCARANKSLAAWGVEG
RVPFLDKEFIDVAMRLNPQDKMCGNGKMEKHILRECFEDDLPEAIAWRQKEQFSDGVGYGWIDSLRETAEVKVTDQQMES
AKFRFPYNTPLTKEAYVYREIFEELFPLEDAAKCVPGGPSIACSSAKAIEWDESFKNCADPSGRAVQAIHNEAYS

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=518, Percent_Identity=39.1891891891892, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI168229252, Length=518, Percent_Identity=39.1891891891892, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI168229250, Length=518, Percent_Identity=39.1891891891892, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI296010848, Length=557, Percent_Identity=37.8815080789946, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI296010852, Length=489, Percent_Identity=38.4458077709611, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI296010850, Length=489, Percent_Identity=38.4458077709611, Blast_Score=293, Evalue=3e-79,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=75.4512635379061, Blast_Score=912, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=571, Percent_Identity=42.7320490367776, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71993933, Length=559, Percent_Identity=42.9338103756708, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17560178, Length=523, Percent_Identity=36.3288718929254, Blast_Score=291, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI25147560, Length=341, Percent_Identity=35.7771260997067, Blast_Score=196, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6321563, Length=545, Percent_Identity=51.3761467889908, Blast_Score=541, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6325403, Length=585, Percent_Identity=48.3760683760684, Blast_Score=533, Evalue=1e-152,
Organism=Drosophila melanogaster, GI45553209, Length=580, Percent_Identity=47.2413793103448, Blast_Score=488, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62793; Mature: 62793

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFGILDIKSDPTPLRKVALEMSKKLRHRGPDWSGIYSSDKAILAHERLAIVGLNSGA
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEECCCCC
QPLYSKDKQHILAVNGEIYNHKELRAKYADDYDFQTDSDCEVILALYRDKGADLLEDLNG
CCCCCCCCCEEEEECCCEECCHHHHHHHCCCCCCCCCCCHHHHHEEHHCCCHHHHHHCCC
IFAFILYDEEKDEYLIGRDHIGIIPMYHGYDEHGNYYIASEMKALVPVCKSMSEFPPGHF
EEEEEEEECCCCCEEECCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
YSSKDAEPTRYYVRDWMEYDAVKDNPTSKDDLKQALEDAVKRQLMTDVPYGVLLSGGLDS
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
SITSAVAKKFAAMRIEDNEKSEAWWPQLHSFAVGLEGAPDLKAAREVANQIGTVHHEMTY
HHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TIQEGLDAIRDVIYHIETYDVTTIRASTPMYLLGRKIKAMGIKMVLSGEGADEIFGGYLY
HHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNKKEFHEETVRKLLALNMFDCARANKSLAAWGVEGRVPFLDKEFIDVAMRLNPQD
EECCCCHHHHHHHHHHHHHHHCHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCC
KMCGNGKMEKHILRECFEDDLPEAIAWRQKEQFSDGVGYGWIDSLRETAEVKVTDQQMES
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEHHHHHC
AKFRFPYNTPLTKEAYVYREIFEELFPLEDAAKCVPGGPSIACSSAKAIEWDESFKNCAD
CEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEECCCCCCCHHHHHHHCCC
PSGRAVQAIHNEAYS
CCCHHHHHHHHHCCC
>Mature Secondary Structure
MCSIFGILDIKSDPTPLRKVALEMSKKLRHRGPDWSGIYSSDKAILAHERLAIVGLNSGA
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEECCCCC
QPLYSKDKQHILAVNGEIYNHKELRAKYADDYDFQTDSDCEVILALYRDKGADLLEDLNG
CCCCCCCCCEEEEECCCEECCHHHHHHHCCCCCCCCCCCHHHHHEEHHCCCHHHHHHCCC
IFAFILYDEEKDEYLIGRDHIGIIPMYHGYDEHGNYYIASEMKALVPVCKSMSEFPPGHF
EEEEEEEECCCCCEEECCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
YSSKDAEPTRYYVRDWMEYDAVKDNPTSKDDLKQALEDAVKRQLMTDVPYGVLLSGGLDS
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
SITSAVAKKFAAMRIEDNEKSEAWWPQLHSFAVGLEGAPDLKAAREVANQIGTVHHEMTY
HHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TIQEGLDAIRDVIYHIETYDVTTIRASTPMYLLGRKIKAMGIKMVLSGEGADEIFGGYLY
HHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNKKEFHEETVRKLLALNMFDCARANKSLAAWGVEGRVPFLDKEFIDVAMRLNPQD
EECCCCHHHHHHHHHHHHHHHCHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCC
KMCGNGKMEKHILRECFEDDLPEAIAWRQKEQFSDGVGYGWIDSLRETAEVKVTDQQMES
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEHHHHHC
AKFRFPYNTPLTKEAYVYREIFEELFPLEDAAKCVPGGPSIACSSAKAIEWDESFKNCAD
CEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEECCCCCCCHHHHHHHCCC
PSGRAVQAIHNEAYS
CCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]