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The map label for this gene is prfC [H]

Identifier: 209694205

GI number: 209694205

Start: 675187

End: 676767

Strand: Direct

Name: prfC [H]

Synonym: VSAL_I0613

Alternate gene names: 209694205

Gene position: 675187-676767 (Clockwise)

Preceding gene: 209694204

Following gene: 209694206

Centisome position: 20.31

GC content: 42.88

Gene sequence:

>1581_bases
ATGTCTTTTTTACAAGAAGTGGGTAAACGTAGAACGTTTGCCATCATTTCTCACCCGGATGCGGGTAAAACAACGATTAC
TGAAAAAGTATTATTATTCGGAAACGCGATTCAAAAAGCGGGTACGGTAAAAGGTCGTGGTTCAAACCAGCACGCAAAAT
CAGACTGGATGGAAATGGAAAAAGAACGTGGTATTTCGGTAACTACGTCAGTAATGCAATTTCCATTTAACGGTTGCCTA
GTTAACCTTCTTGATACTCCTGGGCACGAAGACTTCTCTGAAGATACCTACCGTACATTAACGGCGGTTGATTCTTGCTT
AATGGTTATCGATGCCGCGAAAGGTGTCGAAGATCGTACTCGTAAATTGATGGAAGTAACGCGTTTACGTGATACACCAA
TCGTCACGTTCATGAACAAAATGGACCGTGAAGTACGAGACCCAATGGAAGTGCTTGATGAAGTAGAAAGCGAGCTAGGT
ATGGCTTGTGCTCCTATTTCATGGCCGATTGGTTGTGGTAAAGAATTTAAAGGTGTTTATCACATTCACCGTGATGAAAC
GATCTTGTATCAATCTGGCCATGGCCATGAGATCCAAGAAGTGCGTACGATCAAAGGCCTTGATAACCCAGAGCTTGATG
TTGCAATCGGTAATGAACTTGCGCTCAGTGTTCGAGAAGAGTTAGAGCTGGTTATTGGTGCTGCTCATGAATTTGATTTA
GAGCTATTCCTTAAAGGAGAGCTAACGCCAGTCTACTTTGGTACGGCACTAGGTAACTTCGGTGTTGATCATATGCTTGA
AGGCTTGACGGAATGGGCTCCTGCGCCGCAAACCCGTGAAGCGACAGAGCGTCCAGTTGAAGCGACAGAAGAAAAATTCT
CTGGCTTTGTGTTTAAAATCCAAGCAAACATGGATCCTAAACACCGTGACCGTATCGCCTTTATGCGAATCGTGTCTGGT
ACTTATACTCAAGGCATGAAAATGAACCACGTTCGTACAGGTAAAAACATCAGTATTTCTGATGCGGTAACCTTTATGGC
GGGTGATCGTGCTCGTGCTGAAGTAGCCTATGCGGGTGATATCATTGGCTTGCATAACCACGGTACTATTCAAATTGGTG
ATACCTTTACTCAAGGTGAAAATTTGAAATTCTCAGGGATCCCTAACTTTGCCCCTGAACTGTTCCGTCGTATTCGTCTT
CGTGATCCACTAAAGCAAAAACAATTGCTGAAAGGACTTGTGCAATTATCTGAAGAAGGTGCTGTACAGGTTTTTCGTCC
ATTACAAAACAATGATCTGATCGTTGGTGCGGTTGGTGTTCTTCAGTTTGATGTGGTTGTTTCTCGTTTGAAATCTGAAT
ATAACGTAGAAGCTATTTATGAAGGCATCAGTGTTGCAACCGCTCGTTGGGTTGAATGTTCTGATGGCAAAATGCAAGAT
GAATTCCAGCGTAAGAATCAAACTAACCTAGCATTAGATGGTGGCAATAACTTAACGTATATCGCCCCAACGATGGTTAA
TTTGAATTTAGCGATAGAACGCTTTCCTGATGTTCAATTTAGAGCAACGCGTGAACACTAA

Upstream 100 bases:

>100_bases
CGAAGGTTATTTTAATTCGATGCTTTAGATAATGAACGTCTTTGAGTATAATCTCGATTAATTACATCCTTTCTGAAATC
TTACAGGATTATCAACGCTT

Downstream 100 bases:

>100_bases
TTTATTAAAGTGAATATAATTAAACGGAGCCTATGTAGGCTCCGTTTTTGTATCTATAGTATTGCTGTTTAAAGGGAGAT
GGTGATTATGATTTGGAAGA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSFLQEVGKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEMEKERGISVTTSVMQFPFNGCL
VNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKMDREVRDPMEVLDEVESELG
MACAPISWPIGCGKEFKGVYHIHRDETILYQSGHGHEIQEVRTIKGLDNPELDVAIGNELALSVREELELVIGAAHEFDL
ELFLKGELTPVYFGTALGNFGVDHMLEGLTEWAPAPQTREATERPVEATEEKFSGFVFKIQANMDPKHRDRIAFMRIVSG
TYTQGMKMNHVRTGKNISISDAVTFMAGDRARAEVAYAGDIIGLHNHGTIQIGDTFTQGENLKFSGIPNFAPELFRRIRL
RDPLKQKQLLKGLVQLSEEGAVQVFRPLQNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYEGISVATARWVECSDGKMQD
EFQRKNQTNLALDGGNNLTYIAPTMVNLNLAIERFPDVQFRATREH

Sequences:

>Translated_526_residues
MSFLQEVGKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEMEKERGISVTTSVMQFPFNGCL
VNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKMDREVRDPMEVLDEVESELG
MACAPISWPIGCGKEFKGVYHIHRDETILYQSGHGHEIQEVRTIKGLDNPELDVAIGNELALSVREELELVIGAAHEFDL
ELFLKGELTPVYFGTALGNFGVDHMLEGLTEWAPAPQTREATERPVEATEEKFSGFVFKIQANMDPKHRDRIAFMRIVSG
TYTQGMKMNHVRTGKNISISDAVTFMAGDRARAEVAYAGDIIGLHNHGTIQIGDTFTQGENLKFSGIPNFAPELFRRIRL
RDPLKQKQLLKGLVQLSEEGAVQVFRPLQNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYEGISVATARWVECSDGKMQD
EFQRKNQTNLALDGGNNLTYIAPTMVNLNLAIERFPDVQFRATREH
>Mature_525_residues
SFLQEVGKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEMEKERGISVTTSVMQFPFNGCLV
NLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRTRKLMEVTRLRDTPIVTFMNKMDREVRDPMEVLDEVESELGM
ACAPISWPIGCGKEFKGVYHIHRDETILYQSGHGHEIQEVRTIKGLDNPELDVAIGNELALSVREELELVIGAAHEFDLE
LFLKGELTPVYFGTALGNFGVDHMLEGLTEWAPAPQTREATERPVEATEEKFSGFVFKIQANMDPKHRDRIAFMRIVSGT
YTQGMKMNHVRTGKNISISDAVTFMAGDRARAEVAYAGDIIGLHNHGTIQIGDTFTQGENLKFSGIPNFAPELFRRIRLR
DPLKQKQLLKGLVQLSEEGAVQVFRPLQNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYEGISVATARWVECSDGKMQDE
FQRKNQTNLALDGGNNLTYIAPTMVNLNLAIERFPDVQFRATREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=523, Percent_Identity=26.0038240917782, Blast_Score=163, Evalue=5e-40,
Organism=Homo sapiens, GI19923640, Length=516, Percent_Identity=26.1627906976744, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI25306283, Length=400, Percent_Identity=28.25, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI25306287, Length=488, Percent_Identity=25.2049180327869, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI157426893, Length=173, Percent_Identity=30.635838150289, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=29.9270072992701, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=28.1879194630872, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790835, Length=524, Percent_Identity=79.7709923664122, Blast_Score=902, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=504, Percent_Identity=26.7857142857143, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI48994988, Length=194, Percent_Identity=31.9587628865979, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=32.6923076923077, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=543, Percent_Identity=26.3351749539595, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=179, Percent_Identity=33.5195530726257, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17557151, Length=134, Percent_Identity=32.089552238806, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=29.1925465838509, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=29.3233082706767, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=29.3233082706767, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=478, Percent_Identity=26.3598326359833, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6322359, Length=417, Percent_Identity=25.8992805755396, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=27.2151898734177, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=27.2151898734177, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=24.5033112582781, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24582462, Length=482, Percent_Identity=26.5560165975104, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI221458488, Length=522, Percent_Identity=23.7547892720307, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=28.7581699346405, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=28.7581699346405, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=28.7581699346405, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58855; Mature: 58724

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLQEVGKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEME
CCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
KERGISVTTSVMQFPFNGCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRT
HHCCCEEEHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
RKLMEVTRLRDTPIVTFMNKMDREVRDPMEVLDEVESELGMACAPISWPIGCGKEFKGVY
HHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCEE
HIHRDETILYQSGHGHEIQEVRTIKGLDNPELDVAIGNELALSVREELELVIGAAHEFDL
EEECCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCE
ELFLKGELTPVYFGTALGNFGVDHMLEGLTEWAPAPQTREATERPVEATEEKFSGFVFKI
EEEEECCCCEEEEEHHHCCCCHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHCCEEEEE
QANMDPKHRDRIAFMRIVSGTYTQGMKMNHVRTGKNISISDAVTFMAGDRARAEVAYAGD
ECCCCCCHHHHHHHHHHHCCCCCCCEEECEEECCCCEEHHHHEEEEECCCCCEEEEEECC
IIGLHNHGTIQIGDTFTQGENLKFSGIPNFAPELFRRIRLRDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
AVQVFRPLQNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYEGISVATARWVECSDGKMQD
CEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHEEEEECCCCCCHH
EFQRKNQTNLALDGGNNLTYIAPTMVNLNLAIERFPDVQFRATREH
HHHHCCCCEEEEECCCCEEEEEEEEEEEEEEEECCCCCEEEECCCC
>Mature Secondary Structure 
SFLQEVGKRRTFAIISHPDAGKTTITEKVLLFGNAIQKAGTVKGRGSNQHAKSDWMEME
CHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
KERGISVTTSVMQFPFNGCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDAAKGVEDRT
HHCCCEEEHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
RKLMEVTRLRDTPIVTFMNKMDREVRDPMEVLDEVESELGMACAPISWPIGCGKEFKGVY
HHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCEE
HIHRDETILYQSGHGHEIQEVRTIKGLDNPELDVAIGNELALSVREELELVIGAAHEFDL
EEECCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCE
ELFLKGELTPVYFGTALGNFGVDHMLEGLTEWAPAPQTREATERPVEATEEKFSGFVFKI
EEEEECCCCEEEEEHHHCCCCHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHCCEEEEE
QANMDPKHRDRIAFMRIVSGTYTQGMKMNHVRTGKNISISDAVTFMAGDRARAEVAYAGD
ECCCCCCHHHHHHHHHHHCCCCCCCEEECEEECCCCEEHHHHEEEEECCCCCEEEEEECC
IIGLHNHGTIQIGDTFTQGENLKFSGIPNFAPELFRRIRLRDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
AVQVFRPLQNNDLIVGAVGVLQFDVVVSRLKSEYNVEAIYEGISVATARWVECSDGKMQD
CEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHEEEEECCCCCCHH
EFQRKNQTNLALDGGNNLTYIAPTMVNLNLAIERFPDVQFRATREH
HHHHCCCCEEEEECCCCEEEEEEEEEEEEEEEECCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA