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The map label for this gene is hlyU [H]

Identifier: 209694168

GI number: 209694168

Start: 633619

End: 633930

Strand: Direct

Name: hlyU [H]

Synonym: VSAL_I0576

Alternate gene names: 209694168

Gene position: 633619-633930 (Clockwise)

Preceding gene: 209694167

Following gene: 209694170

Centisome position: 19.06

GC content: 33.97

Gene sequence:

>312_bases
ATGGCAGGTATATTTATGGATTTACAACAAATGGAGAAAAACGCTCCGCAAGCAGTCACACTACTAAAAGCAATGGCTAA
TGAACGTCGATTATTTATTCTTTGTTTATTGCTGGAAGGTGAACTTTCAGTAGGTCAAATAGCAGAACAGTTGTCATTAA
GTCAATCTGCATTGTCTCAACATCTTGGTTGGTTGCGTAAAGATGCGTTGGTTTCGACAAGAAAAGAGTCTCAAACTGTG
TTTTATTCATTAAGCAGTCATGAAGTTAAATCAGTAATCCAATTATTACATAATTTATATTGTTCAAAATAA

Upstream 100 bases:

>100_bases
CGGCAGTAAAAAGTATTTGTGAAGCTGATTTTTCACAGTTATTTGATTTTAATTTGCAAAAATAGAATGCTTTAAGCTAA
ATTAGCAGGAACTAAATTAG

Downstream 100 bases:

>100_bases
AACAGAATTAACGTAGATAAGGAAGATGAAGAGGTGACACTCTTTGTTTTCTAAATAACAGACATAAAAAAACCGACCTA
GGTCGGTTTTTTCTTATTTC

Product: transcriptional activator HlyU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 103; Mature: 102

Protein sequence:

>103_residues
MAGIFMDLQQMEKNAPQAVTLLKAMANERRLFILCLLLEGELSVGQIAEQLSLSQSALSQHLGWLRKDALVSTRKESQTV
FYSLSSHEVKSVIQLLHNLYCSK

Sequences:

>Translated_103_residues
MAGIFMDLQQMEKNAPQAVTLLKAMANERRLFILCLLLEGELSVGQIAEQLSLSQSALSQHLGWLRKDALVSTRKESQTV
FYSLSSHEVKSVIQLLHNLYCSK
>Mature_102_residues
AGIFMDLQQMEKNAPQAVTLLKAMANERRLFILCLLLEGELSVGQIAEQLSLSQSALSQHLGWLRKDALVSTRKESQTVF
YSLSSHEVKSVIQLLHNLYCSK

Specific function: Up-regulates the expression of the hemolysin gene, hlyA, and may promote expression of other virulence determinants in vivo. It may have both positive and negative regulator activities [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=98, Percent_Identity=37.7551020408163, Blast_Score=77, Evalue=2e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 11578; Mature: 11447

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGIFMDLQQMEKNAPQAVTLLKAMANERRLFILCLLLEGELSVGQIAEQLSLSQSALSQ
CCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHEEHHCCCCCHHHHHHHHHHHHHHHHH
HLGWLRKDALVSTRKESQTVFYSLSSHEVKSVIQLLHNLYCSK
HHHHHHHHHHHHHCCCCCHHEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGIFMDLQQMEKNAPQAVTLLKAMANERRLFILCLLLEGELSVGQIAEQLSLSQSALSQ
CCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHEEHHCCCCCHHHHHHHHHHHHHHHHH
HLGWLRKDALVSTRKESQTVFYSLSSHEVKSVIQLLHNLYCSK
HHHHHHHHHHHHHCCCCCHHEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8231807; 9457888; 10952301 [H]