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The map label for this gene is thyA [H]

Identifier: 209694165

GI number: 209694165

Start: 630234

End: 631091

Strand: Direct

Name: thyA [H]

Synonym: VSAL_I0573

Alternate gene names: 209694165

Gene position: 630234-631091 (Clockwise)

Preceding gene: 209694164

Following gene: 209694167

Centisome position: 18.95

GC content: 39.51

Gene sequence:

>858_bases
ATGACCGTGAAGCAGTATTTAGACTTATGCCAACGTATCGTTAGTGAAGGGACTTGGATTGAGAACGAGCGCACCGGAAA
GCGTTGTTTGACGGTAATTAATGCCGATTTAACTTACGATGTTGCCAATGGTGAATTTCCTTTAGTGACAACTCGCAAGA
GTTTCTGGAAATCTGCGGTTGCCGAAATGATTGGTTATATTCGTGGTTATGATAATGCCGAAGATTTTCGTCAGATTGGT
ACTAAAACATGGGATGCCAATGCGAACTTAAATAATGCTTGGTTAAATAACCCTCACCGTAAAGGTGAAGATGACATGGG
ACGTGTATATGGCGTTCAAGGTCGTTCATGGGCAAAACCGAATGGTGAGTTCGTTGATCAACTAAAAAAGATCATTGATG
ATTTAAGTCAAGGCATTGATGACCGGGGTGAAATTTTAAATTTCTATAATCCGGGTGAGTTTGATATAGGGTGTTTGCGC
CCGTGCATGTACTCACACCACTTTTCACTATTAGGTGACACATTATACTTAAATAGTACTCAACGCTCTTGTGATGTCCC
TCTAGGCTTAAATTTCAATATGGTTCAGGTGTATTTTCTACTGGCTATCGTTGCTCAAATTACAGGACATAAAGCCGGTA
AAGCGTACCATAAGATTGTGAACGGTCACATTTACGAAGATCAACTTGAATTGATGAAAGACGTTCAGTTAAAGCGTGAA
CCGTTGAAAGCGCCGGTCTTTAAGATTAACCCTAAGATTACTTCTTTAGAAGATTTAGAAACCTGGGTCACAATGGATGA
TTTTGAAGTGATTGGCTATGAAAGTCATGAGGTAATAAAATACCCATTTTCAGTATAA

Upstream 100 bases:

>100_bases
CTCAAAACAAAAAGCAAAATAAGCTAGAATAGCGATAATAACGATTTTTAGTAATGGCCTCTTCGTTAGAGGCCATTTTT
TTAAGCCAATTTTAGAGAGC

Downstream 100 bases:

>100_bases
TAGGTACTGAAAAATAAAAAGCCCCAGAGTGATTACCACTCTGGGGCTTTTTTATTTATCTAATCAGTTTACACGGTTAA
CGCTAAAACACTGCCAGGAA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTVKQYLDLCQRIVSEGTWIENERTGKRCLTVINADLTYDVANGEFPLVTTRKSFWKSAVAEMIGYIRGYDNAEDFRQIG
TKTWDANANLNNAWLNNPHRKGEDDMGRVYGVQGRSWAKPNGEFVDQLKKIIDDLSQGIDDRGEILNFYNPGEFDIGCLR
PCMYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYFLLAIVAQITGHKAGKAYHKIVNGHIYEDQLELMKDVQLKRE
PLKAPVFKINPKITSLEDLETWVTMDDFEVIGYESHEVIKYPFSV

Sequences:

>Translated_285_residues
MTVKQYLDLCQRIVSEGTWIENERTGKRCLTVINADLTYDVANGEFPLVTTRKSFWKSAVAEMIGYIRGYDNAEDFRQIG
TKTWDANANLNNAWLNNPHRKGEDDMGRVYGVQGRSWAKPNGEFVDQLKKIIDDLSQGIDDRGEILNFYNPGEFDIGCLR
PCMYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYFLLAIVAQITGHKAGKAYHKIVNGHIYEDQLELMKDVQLKRE
PLKAPVFKINPKITSLEDLETWVTMDDFEVIGYESHEVIKYPFSV
>Mature_284_residues
TVKQYLDLCQRIVSEGTWIENERTGKRCLTVINADLTYDVANGEFPLVTTRKSFWKSAVAEMIGYIRGYDNAEDFRQIGT
KTWDANANLNNAWLNNPHRKGEDDMGRVYGVQGRSWAKPNGEFVDQLKKIIDDLSQGIDDRGEILNFYNPGEFDIGCLRP
CMYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYFLLAIVAQITGHKAGKAYHKIVNGHIYEDQLELMKDVQLKREP
LKAPVFKINPKITSLEDLETWVTMDDFEVIGYESHEVIKYPFSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=289, Percent_Identity=33.2179930795848, Blast_Score=158, Evalue=7e-39,
Organism=Escherichia coli, GI1789191, Length=283, Percent_Identity=31.8021201413428, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71993377, Length=276, Percent_Identity=28.2608695652174, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324648, Length=303, Percent_Identity=26.0726072607261, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17137556, Length=293, Percent_Identity=27.9863481228669, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 32646; Mature: 32515

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKQYLDLCQRIVSEGTWIENERTGKRCLTVINADLTYDVANGEFPLVTTRKSFWKSAV
CCHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEEECCEEEEECCCCCCEEECHHHHHHHHH
AEMIGYIRGYDNAEDFRQIGTKTWDANANLNNAWLNNPHRKGEDDMGRVYGVQGRSWAKP
HHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCHHHEEECCCCCCCCC
NGEFVDQLKKIIDDLSQGIDDRGEILNFYNPGEFDIGCLRPCMYSHHFSLLGDTLYLNST
CHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCC
QRSCDVPLGLNFNMVQVYFLLAIVAQITGHKAGKAYHKIVNGHIYEDQLELMKDVQLKRE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHC
PLKAPVFKINPKITSLEDLETWVTMDDFEVIGYESHEVIKYPFSV
CCCCCEEEECCCCCCHHHHHHHEEECCEEEECCCCCCEEECCCCC
>Mature Secondary Structure 
TVKQYLDLCQRIVSEGTWIENERTGKRCLTVINADLTYDVANGEFPLVTTRKSFWKSAV
CHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEEECCEEEEECCCCCCEEECHHHHHHHHH
AEMIGYIRGYDNAEDFRQIGTKTWDANANLNNAWLNNPHRKGEDDMGRVYGVQGRSWAKP
HHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCHHHEEECCCCCCCCC
NGEFVDQLKKIIDDLSQGIDDRGEILNFYNPGEFDIGCLRPCMYSHHFSLLGDTLYLNST
CHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCC
QRSCDVPLGLNFNMVQVYFLLAIVAQITGHKAGKAYHKIVNGHIYEDQLELMKDVQLKRE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHC
PLKAPVFKINPKITSLEDLETWVTMDDFEVIGYESHEVIKYPFSV
CCCCCEEEECCCCCCHHHHHHHEEECCEEEECCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA