Click here to switch to the map view.

The map label for this gene is ptsP [H]

Identifier: 209694162

GI number: 209694162

Start: 626228

End: 628489

Strand: Direct

Name: ptsP [H]

Synonym: VSAL_I0570

Alternate gene names: 209694162

Gene position: 626228-628489 (Clockwise)

Preceding gene: 209694161

Following gene: 209694163

Centisome position: 18.83

GC content: 39.61

Gene sequence:

>2262_bases
ATGCTTACTCAACTCAGGGATATTGTTGACCAAGTCGCTAAAGCGGAATCACTGGATGATGCTTTAGCGTTATTGGTTAA
AAGTACGCGCCATTCAATGCGCACTGAATGTTGCTCAGTTTATCTTATGAGTAATGACAGCCTGCGCTTAGAATTAATGG
CAACAGAAGGCCTGAAGGCCAAACCTAACACGGTTTCCTTAAGTATTGATGAAGGCCTTGTGGGTTTAGTTGCGCGCAGC
TGTGAGCCTCTTAACTTAGCAAATGCCTCTGTTCACCCCTCATACAAATACATACCTAGTATTGCTGAATATAAATTTAA
TTCTTTTTTGGCGACACCGATTATTTATCGACGTCAAAATCTTGGGGTTCTGGTTGTTCAGCAAAAAGAAAAACGTCAAT
TCACCGAAGTAGAAGAATCCTTTATTGTGACTCTGTCGGCGCAATTGGCTGTGGTTCTTGCTCACGCTAAAGCACAAGAG
AGCTGGTCAAGCCAACTTCAACTAACGTCTGTTAATCAAAAGCAATTTAAAGGATTGTCAGCATCGACTGGAGTGGCTAT
CGCCCCTTTGTGGTTTGATGATGCTCAACCTAACGTTGAAGCGGTTTTACCGAGTTCTTGTATTGATATAGAAAAAGAAC
AAGATCGACTTGGTAGTGCTATTGAGTTTGCATTAAAAGATTTTCGTCGAGCAAAAAAGAGATTTGAACAAGAGTTAAAT
ACAGAAACCTTAGCTATTTTTGATCTGTTTACCCACTTATTGAATGATCCGATGTTGAAGGGGGATCTTAAAAAACAGAT
CAACCAAGGAGATTCTGCTGAGTGGGCATTACGTCAGGTTATTGAGTCTTATGCCGCGCGTTTTGGACGCATGAGCGACT
CTTATTTAAGTGAAAGAGCGAATGATGTTCGAGAATTAGGGCAGCGTTTACTTTATTTCTTAAATTATGAGAATAAATTT
GATCTCGAACTCTCGGAACCAGTCATCTTAGTAACGACCCAGCTAACAGCGTCGATGCTTGCCAGTATTCCTAGAGAAAA
ATTAAAGGCGGTTATCTCATTAGAAGGCGCGGCGAACTCTCACGCGGCAATTCTATCAAGAGCGCTTGGTATTCCTGCGA
TCATGGGGGTTGAGTTTAATCCTGCGCATTATCATCAAAAGCTTGCCATCGTTGATGGCTATAGTGGCATGCTATACATA
AAGCCGACAGAGGCTTTAGTTCAAGAATATATTGAACTGCAAAATGAAGAATTAGCCTTATCTCAGCTTATTACTCTGGA
TTTAGATAAGCCCACCTTTACTCGTGATGCCCATCAAATGAGAATACATTTGAATGCAGGTCTCAGCGCCGACACCACGA
TTGCTGTAAACCAAGGTGTTGATGGTGTTGGTCTTTATCGAACGGAAATCCCGTTTCTTTTACAGCAGCGCTTTCCTTCT
GAAGAAGAGCAATATCTTCAATATAAATCGATTTTAGAAACCTATATTGATAAACCCGTCATCATGAGAACATTAGATAT
TGGCGGGGATAAACCATTACCTTATTTACCAATAGAAGAAGACAACCCATTTTTAGGTTGGCGCGGGATCCGTTTTACTT
TGGATCATCCTGATATTTTCTTATTACAGTTAAAAGCAATGGTAAGAGCGAATATCGGTAATAATAATCTTTCTATCATG
TTACCGATGATTTCTGGTATTCCAGAGCTAAAACAATCAAGAAAGTTGATCAATCAAGCTTATGAAGAAGTATTGAATGA
ATCGGGTGTTTCTTTTGTTCTACCTCGGATTGGTATGATGATCGAAGTGCCGTCTATGTTGTATTTACTCCCAAGAATTA
CTGAGTTGGTGGATTTTATTTCTGTCGGGACAAATGATTTAACACAATATTTACTCGCGGTTGATCGTAATAATTCAAGA
GTTTCTCATGTGTATGAGTCATATCATCCTGCGGTGCTATTGGCTTTAAAGCAGATTATCGATACGGCTCAGCAATATAA
TTTACCGGTGAGTGTGTGTGGTGAGTTGGCTGGCGATCCTATTGGCTGTTTATTGTTGATGGGATTAGGTTACGAACATC
TCAGCATGAATACCTCTAACGTGGCCAAAGTAAAATACATAATTCGTAAAGTTACATTAGAAGAAGTAAAAGAGATTGTT
GAAAAACTTTTACACCATGATGATGCAGAGAGTATTTTGGCTGATATGATGGTCTTTTTTGAAAAGCATGATCTTTCAGG
ATTCATCAGAGCAGGAAAATAA

Upstream 100 bases:

>100_bases
GTTTGCGGCAATTGCAATGCCGTTTAAAGAACGAAAATTTAAAAGGAAAGGTAAAAAAGGGTAAGCTGTCTATACCTTAT
AGCCTTAAGGATTGGTCAGT

Downstream 100 bases:

>100_bases
ATGGTCATTAGTGTATGGGAGCTATTTGCTCTCTATCTTGCCTTAGGCAGTATTGTTGGTGTTATGGCAGGGTTATTAGG
GATTGGCGGGGGCTTATTAG

Product: fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 753; Mature: 753

Protein sequence:

>753_residues
MLTQLRDIVDQVAKAESLDDALALLVKSTRHSMRTECCSVYLMSNDSLRLELMATEGLKAKPNTVSLSIDEGLVGLVARS
CEPLNLANASVHPSYKYIPSIAEYKFNSFLATPIIYRRQNLGVLVVQQKEKRQFTEVEESFIVTLSAQLAVVLAHAKAQE
SWSSQLQLTSVNQKQFKGLSASTGVAIAPLWFDDAQPNVEAVLPSSCIDIEKEQDRLGSAIEFALKDFRRAKKRFEQELN
TETLAIFDLFTHLLNDPMLKGDLKKQINQGDSAEWALRQVIESYAARFGRMSDSYLSERANDVRELGQRLLYFLNYENKF
DLELSEPVILVTTQLTASMLASIPREKLKAVISLEGAANSHAAILSRALGIPAIMGVEFNPAHYHQKLAIVDGYSGMLYI
KPTEALVQEYIELQNEELALSQLITLDLDKPTFTRDAHQMRIHLNAGLSADTTIAVNQGVDGVGLYRTEIPFLLQQRFPS
EEEQYLQYKSILETYIDKPVIMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFLLQLKAMVRANIGNNNLSIM
LPMISGIPELKQSRKLINQAYEEVLNESGVSFVLPRIGMMIEVPSMLYLLPRITELVDFISVGTNDLTQYLLAVDRNNSR
VSHVYESYHPAVLLALKQIIDTAQQYNLPVSVCGELAGDPIGCLLLMGLGYEHLSMNTSNVAKVKYIIRKVTLEEVKEIV
EKLLHHDDAESILADMMVFFEKHDLSGFIRAGK

Sequences:

>Translated_753_residues
MLTQLRDIVDQVAKAESLDDALALLVKSTRHSMRTECCSVYLMSNDSLRLELMATEGLKAKPNTVSLSIDEGLVGLVARS
CEPLNLANASVHPSYKYIPSIAEYKFNSFLATPIIYRRQNLGVLVVQQKEKRQFTEVEESFIVTLSAQLAVVLAHAKAQE
SWSSQLQLTSVNQKQFKGLSASTGVAIAPLWFDDAQPNVEAVLPSSCIDIEKEQDRLGSAIEFALKDFRRAKKRFEQELN
TETLAIFDLFTHLLNDPMLKGDLKKQINQGDSAEWALRQVIESYAARFGRMSDSYLSERANDVRELGQRLLYFLNYENKF
DLELSEPVILVTTQLTASMLASIPREKLKAVISLEGAANSHAAILSRALGIPAIMGVEFNPAHYHQKLAIVDGYSGMLYI
KPTEALVQEYIELQNEELALSQLITLDLDKPTFTRDAHQMRIHLNAGLSADTTIAVNQGVDGVGLYRTEIPFLLQQRFPS
EEEQYLQYKSILETYIDKPVIMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFLLQLKAMVRANIGNNNLSIM
LPMISGIPELKQSRKLINQAYEEVLNESGVSFVLPRIGMMIEVPSMLYLLPRITELVDFISVGTNDLTQYLLAVDRNNSR
VSHVYESYHPAVLLALKQIIDTAQQYNLPVSVCGELAGDPIGCLLLMGLGYEHLSMNTSNVAKVKYIIRKVTLEEVKEIV
EKLLHHDDAESILADMMVFFEKHDLSGFIRAGK
>Mature_753_residues
MLTQLRDIVDQVAKAESLDDALALLVKSTRHSMRTECCSVYLMSNDSLRLELMATEGLKAKPNTVSLSIDEGLVGLVARS
CEPLNLANASVHPSYKYIPSIAEYKFNSFLATPIIYRRQNLGVLVVQQKEKRQFTEVEESFIVTLSAQLAVVLAHAKAQE
SWSSQLQLTSVNQKQFKGLSASTGVAIAPLWFDDAQPNVEAVLPSSCIDIEKEQDRLGSAIEFALKDFRRAKKRFEQELN
TETLAIFDLFTHLLNDPMLKGDLKKQINQGDSAEWALRQVIESYAARFGRMSDSYLSERANDVRELGQRLLYFLNYENKF
DLELSEPVILVTTQLTASMLASIPREKLKAVISLEGAANSHAAILSRALGIPAIMGVEFNPAHYHQKLAIVDGYSGMLYI
KPTEALVQEYIELQNEELALSQLITLDLDKPTFTRDAHQMRIHLNAGLSADTTIAVNQGVDGVGLYRTEIPFLLQQRFPS
EEEQYLQYKSILETYIDKPVIMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFLLQLKAMVRANIGNNNLSIM
LPMISGIPELKQSRKLINQAYEEVLNESGVSFVLPRIGMMIEVPSMLYLLPRITELVDFISVGTNDLTQYLLAVDRNNSR
VSHVYESYHPAVLLALKQIIDTAQQYNLPVSVCGELAGDPIGCLLLMGLGYEHLSMNTSNVAKVKYIIRKVTLEEVKEIV
EKLLHHDDAESILADMMVFFEKHDLSGFIRAGK

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=758, Percent_Identity=48.8126649076517, Blast_Score=736, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=481, Percent_Identity=37.6299376299376, Blast_Score=311, Evalue=8e-86,
Organism=Escherichia coli, GI1788726, Length=577, Percent_Identity=32.2357019064125, Blast_Score=267, Evalue=2e-72,
Organism=Escherichia coli, GI48994992, Length=581, Percent_Identity=30.9810671256454, Blast_Score=262, Evalue=6e-71,
Organism=Escherichia coli, GI1787994, Length=415, Percent_Identity=26.2650602409639, Blast_Score=97, Evalue=6e-21,
Organism=Escherichia coli, GI226510935, Length=177, Percent_Identity=29.3785310734463, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 84516; Mature: 84516

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQLRDIVDQVAKAESLDDALALLVKSTRHSMRTECCSVYLMSNDSLRLELMATEGLKA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEECCCCCC
KPNTVSLSIDEGLVGLVARSCEPLNLANASVHPSYKYIPSIAEYKFNSFLATPIIYRRQN
CCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCHHHEEECC
LGVLVVQQKEKRQFTEVEESFIVTLSAQLAVVLAHAKAQESWSSQLQLTSVNQKQFKGLS
CCEEEEECHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHCCCC
ASTGVAIAPLWFDDAQPNVEAVLPSSCIDIEKEQDRLGSAIEFALKDFRRAKKRFEQELN
CCCCCEEEEEEECCCCCCHHEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TETLAIFDLFTHLLNDPMLKGDLKKQINQGDSAEWALRQVIESYAARFGRMSDSYLSERA
CHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
NDVRELGQRLLYFLNYENKFDLELSEPVILVTTQLTASMLASIPREKLKAVISLEGAANS
HHHHHHHHHHHHHCCCCCEECCEECCCEEEEEHHHHHHHHHHCCHHHHHHHHEECCCCCC
HAAILSRALGIPAIMGVEFNPAHYHQKLAIVDGYSGMLYIKPTEALVQEYIELQNEELAL
HHHHHHHHHCCCEEECCCCCCHHHHCEEEEEECCCCEEEECCHHHHHHHHHHHCCCHHHH
SQLITLDLDKPTFTRDAHQMRIHLNAGLSADTTIAVNQGVDGVGLYRTEIPFLLQQRFPS
HHHHEEECCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCEEEHHHHHHHHHHHCCC
EEEQYLQYKSILETYIDKPVIMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIF
CHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECCCCCCCEEEEECEEEEECCCCCH
LLQLKAMVRANIGNNNLSIMLPMISGIPELKQSRKLINQAYEEVLNESGVSFVLPRIGMM
HHHHHHHHHHCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCE
IEVPSMLYLLPRITELVDFISVGTNDLTQYLLAVDRNNSRVSHVYESYHPAVLLALKQII
EEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHH
DTAQQYNLPVSVCGELAGDPIGCLLLMGLGYEHLSMNTSNVAKVKYIIRKVTLEEVKEIV
HHHHHCCCCHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
EKLLHHDDAESILADMMVFFEKHDLSGFIRAGK
HHHHCCCCHHHHHHHHHHHHHHCCCCCCEECCC
>Mature Secondary Structure
MLTQLRDIVDQVAKAESLDDALALLVKSTRHSMRTECCSVYLMSNDSLRLELMATEGLKA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEECCCCCC
KPNTVSLSIDEGLVGLVARSCEPLNLANASVHPSYKYIPSIAEYKFNSFLATPIIYRRQN
CCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCHHHEEECC
LGVLVVQQKEKRQFTEVEESFIVTLSAQLAVVLAHAKAQESWSSQLQLTSVNQKQFKGLS
CCEEEEECHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHCCCC
ASTGVAIAPLWFDDAQPNVEAVLPSSCIDIEKEQDRLGSAIEFALKDFRRAKKRFEQELN
CCCCCEEEEEEECCCCCCHHEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TETLAIFDLFTHLLNDPMLKGDLKKQINQGDSAEWALRQVIESYAARFGRMSDSYLSERA
CHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
NDVRELGQRLLYFLNYENKFDLELSEPVILVTTQLTASMLASIPREKLKAVISLEGAANS
HHHHHHHHHHHHHCCCCCEECCEECCCEEEEEHHHHHHHHHHCCHHHHHHHHEECCCCCC
HAAILSRALGIPAIMGVEFNPAHYHQKLAIVDGYSGMLYIKPTEALVQEYIELQNEELAL
HHHHHHHHHCCCEEECCCCCCHHHHCEEEEEECCCCEEEECCHHHHHHHHHHHCCCHHHH
SQLITLDLDKPTFTRDAHQMRIHLNAGLSADTTIAVNQGVDGVGLYRTEIPFLLQQRFPS
HHHHEEECCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCEEEHHHHHHHHHHHCCC
EEEQYLQYKSILETYIDKPVIMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIF
CHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECCCCCCCEEEEECEEEEECCCCCH
LLQLKAMVRANIGNNNLSIMLPMISGIPELKQSRKLINQAYEEVLNESGVSFVLPRIGMM
HHHHHHHHHHCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCE
IEVPSMLYLLPRITELVDFISVGTNDLTQYLLAVDRNNSRVSHVYESYHPAVLLALKQII
EEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHH
DTAQQYNLPVSVCGELAGDPIGCLLLMGLGYEHLSMNTSNVAKVKYIIRKVTLEEVKEIV
HHHHHCCCCHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
EKLLHHDDAESILADMMVFFEKHDLSGFIRAGK
HHHHCCCCHHHHHHHHHHHHHHCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]