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The map label for this gene is nudH [H]

Identifier: 209694161

GI number: 209694161

Start: 625679

End: 626191

Strand: Direct

Name: nudH [H]

Synonym: VSAL_I0569

Alternate gene names: 209694161

Gene position: 625679-626191 (Clockwise)

Preceding gene: 209694159

Following gene: 209694162

Centisome position: 18.82

GC content: 41.33

Gene sequence:

>513_bases
GTGATAGATGGCGATGGTTTCCGCCCGAATGTCGGGATAGTGATATGCAATAGCCATGGTCAAGTATTCTGGGCGAAGCG
ATATGGACAGCATTCATGGCAGTTTCCGCAAGGTGGTATTGATGACGGTGAAACTCCAGAGCAAGCCATGTATCGTGAAT
TGTATGAAGAAGTCGGTCTAACTAAGAATGATGTACGTATTTTAGCAAGTAGCCGTCATTGGTTACGCTATAAACTGCCA
AAACGTTTAGTTAGATGGGATTCAAAGCCTGTTTGTATTGGACAGAAACAGAAATGGTTTTTGTTACGCTTAGAATGTGA
TGAATCTAAGGTGAACATGCAAAGAGATCGCTCACCTGAGTTTGATGGTTGGCGATGGGTAAGTTATTGGTACCCAGTAA
GACAAGTTGTATCATTTAAGCGAGATGTTTATCGTCGAGCTCTTAAAGAGTTTGCGGCAATTGCAATGCCGTTTAAAGAA
CGAAAATTTAAAAGGAAAGGTAAAAAAGGGTAA

Upstream 100 bases:

>100_bases
TTATTTTAGTAAAACGTGACAAGAAAGCGTGATCCTGTTTGCTCAAAGAGACGATCTGTGAAAAAATCAAGGGAATATAA
GTTTATGATGAGGTCATCCT

Downstream 100 bases:

>100_bases
GCTGTCTATACCTTATAGCCTTAAGGATTGGTCAGTATGCTTACTCAACTCAGGGATATTGTTGACCAAGTCGCTAAAGC
GGAATCACTGGATGATGCTT

Product: dinucleoside polyphosphate hydrolase

Products: GTP; GMP

Alternate protein names: (Di)nucleoside polyphosphate hydrolase [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MIDGDGFRPNVGIVICNSHGQVFWAKRYGQHSWQFPQGGIDDGETPEQAMYRELYEEVGLTKNDVRILASSRHWLRYKLP
KRLVRWDSKPVCIGQKQKWFLLRLECDESKVNMQRDRSPEFDGWRWVSYWYPVRQVVSFKRDVYRRALKEFAAIAMPFKE
RKFKRKGKKG

Sequences:

>Translated_170_residues
MIDGDGFRPNVGIVICNSHGQVFWAKRYGQHSWQFPQGGIDDGETPEQAMYRELYEEVGLTKNDVRILASSRHWLRYKLP
KRLVRWDSKPVCIGQKQKWFLLRLECDESKVNMQRDRSPEFDGWRWVSYWYPVRQVVSFKRDVYRRALKEFAAIAMPFKE
RKFKRKGKKG
>Mature_170_residues
MIDGDGFRPNVGIVICNSHGQVFWAKRYGQHSWQFPQGGIDDGETPEQAMYRELYEEVGLTKNDVRILASSRHWLRYKLP
KRLVRWDSKPVCIGQKQKWFLLRLECDESKVNMQRDRSPEFDGWRWVSYWYPVRQVVSFKRDVYRRALKEFAAIAMPFKE
RKFKRKGKKG

Specific function: Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789194, Length=164, Percent_Identity=75, Blast_Score=271, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797
- InterPro:   IPR022927 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.17

Molecular weight: Translated: 20349; Mature: 20349

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDGDGFRPNVGIVICNSHGQVFWAKRYGQHSWQFPQGGIDDGETPEQAMYRELYEEVGL
CCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
TKNDVRILASSRHWLRYKLPKRLVRWDSKPVCIGQKQKWFLLRLECDESKVNMQRDRSPE
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEECCCCCCCHHHCCCCC
FDGWRWVSYWYPVRQVVSFKRDVYRRALKEFAAIAMPFKERKFKRKGKKG
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
>Mature Secondary Structure
MIDGDGFRPNVGIVICNSHGQVFWAKRYGQHSWQFPQGGIDDGETPEQAMYRELYEEVGL
CCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
TKNDVRILASSRHWLRYKLPKRLVRWDSKPVCIGQKQKWFLLRLECDESKVNMQRDRSPE
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEECCCCCCCHHHCCCCC
FDGWRWVSYWYPVRQVVSFKRDVYRRALKEFAAIAMPFKERKFKRKGKKG
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: P1,P4-bis(5'-guanosyl) tetraphosphate; H2O

Specific reaction: P1,P4-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA