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The map label for this gene is pyrB

Identifier: 209694112

GI number: 209694112

Start: 573106

End: 574035

Strand: Reverse

Name: pyrB

Synonym: VSAL_I0519

Alternate gene names: 209694112

Gene position: 574035-573106 (Counterclockwise)

Preceding gene: 209694113

Following gene: 209694111

Centisome position: 17.26

GC content: 40.0

Gene sequence:

>930_bases
ATGGCTAACTCGCTATATCAAAAACATATCATTTCTATTCCAGAGCTATCTCGCGAAGAGTTAGAGCTGATTGTGGAAAC
GGCAGGTAATATAAAAAAAGAACCTCAACCTGATTTGTTAAAAAACAAAGTGATTGCGAGCTGCTTTTTTGAAGCATCCA
CGCGAACGCGTCTTTCTTTCGAAACCGCCATTCAACGCTTAGGTGGATCTGTCATTGGTTTTGATAGTGCAGGAAATACT
TCCCTTGCTCAAAAAGGTGAGACGCTAGCCGATTCAGTTCAAATCATTGCATCTTATGCTAATGCTTATGTTATGCGTCA
CCCTAGAGAAGGTGCAGCACGTCTTGCTTCTGAGTTTTCAAACGGCACCCCGGTAATTAATGCAGGAGATGGTGCAAACC
AACACCCAACTCAGACCCTCTTGGATCTTTATACTATCTACGAAACTCAAGGACGCTTAGATAATCTAAATATCGCTTTT
GTTGGTGATTTAAAATATGGCCGAACGGTTCACTCTTTAACTCAAGCATTGGCCAAGTTTAATGGCGTTAAATTCTTTTT
TATTGCTCCAGAAGTATTAGCGATGCCTGATTACATTTGTGAAGAGTTAGATGAACTTGGTATTGAATACCAACTGCTAA
ATAGCATGGAAGAAGCCATTCCTGAACTCGATATTCTATACATGACTCGAGTTCAAAAAGAGCGCTTTGATGAATCAGAA
TACGCACACATTAAGTCTGCATACATCTTATCTGCTGCGGATCTAAAACCTGCACGTGAAAACCTAAAAGTGCTTCACCC
TCTTCCTCGTATTGATGAAATTAATATCGACGTCGATAAAACACCACACGCGTATTATTTCCAACAAGCTGAAAATGGGG
TTTATGCTCGCCAAGCATTACTTGCTCTTGTTTTGAATGAATCACTATAA

Upstream 100 bases:

>100_bases
TATTTTTTGAGTTTCCTAATTACCGATATTTTTAAAGCCTCCTATTTTATAGGGGGCTTTTTTTTGATTTCAATATGATA
AATAAAAGGGAAGAGAGACT

Downstream 100 bases:

>100_bases
GGAAGAAAATCAAATGTCGAATAAAACTGAATTACGTGTTGAAGCAATCAAAAACGGCACCGTTATCGATCACATTCCTG
CAAATATTGGTGTAAAAGTG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MANSLYQKHIISIPELSREELELIVETAGNIKKEPQPDLLKNKVIASCFFEASTRTRLSFETAIQRLGGSVIGFDSAGNT
SLAQKGETLADSVQIIASYANAYVMRHPREGAARLASEFSNGTPVINAGDGANQHPTQTLLDLYTIYETQGRLDNLNIAF
VGDLKYGRTVHSLTQALAKFNGVKFFFIAPEVLAMPDYICEELDELGIEYQLLNSMEEAIPELDILYMTRVQKERFDESE
YAHIKSAYILSAADLKPARENLKVLHPLPRIDEINIDVDKTPHAYYFQQAENGVYARQALLALVLNESL

Sequences:

>Translated_309_residues
MANSLYQKHIISIPELSREELELIVETAGNIKKEPQPDLLKNKVIASCFFEASTRTRLSFETAIQRLGGSVIGFDSAGNT
SLAQKGETLADSVQIIASYANAYVMRHPREGAARLASEFSNGTPVINAGDGANQHPTQTLLDLYTIYETQGRLDNLNIAF
VGDLKYGRTVHSLTQALAKFNGVKFFFIAPEVLAMPDYICEELDELGIEYQLLNSMEEAIPELDILYMTRVQKERFDESE
YAHIKSAYILSAADLKPARENLKVLHPLPRIDEINIDVDKTPHAYYFQQAENGVYARQALLALVLNESL
>Mature_308_residues
ANSLYQKHIISIPELSREELELIVETAGNIKKEPQPDLLKNKVIASCFFEASTRTRLSFETAIQRLGGSVIGFDSAGNTS
LAQKGETLADSVQIIASYANAYVMRHPREGAARLASEFSNGTPVINAGDGANQHPTQTLLDLYTIYETQGRLDNLNIAFV
GDLKYGRTVHSLTQALAKFNGVKFFFIAPEVLAMPDYICEELDELGIEYQLLNSMEEAIPELDILYMTRVQKERFDESEY
AHIKSAYILSAADLKPARENLKVLHPLPRIDEINIDVDKTPHAYYFQQAENGVYARQALLALVLNESL

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=45.016077170418, Blast_Score=271, Evalue=5e-73,
Organism=Homo sapiens, GI38788445, Length=319, Percent_Identity=26.6457680250784, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=69.9029126213592, Blast_Score=470, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI193204318, Length=326, Percent_Identity=40.4907975460123, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6322331, Length=309, Percent_Identity=39.4822006472492, Blast_Score=229, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6322373, Length=325, Percent_Identity=25.5384615384615, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24642586, Length=312, Percent_Identity=43.2692307692308, Blast_Score=257, Evalue=9e-69,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_ALISL (B6EMS5)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262040.1
- GeneID:   6989054
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0519
- HOGENOM:   HBG579429
- OMA:   FKQSFYG
- ProtClustDB:   PRK00856
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34489; Mature: 34357

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSLYQKHIISIPELSREELELIVETAGNIKKEPQPDLLKNKVIASCFFEASTRTRLSF
CCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
ETAIQRLGGSVIGFDSAGNTSLAQKGETLADSVQIIASYANAYVMRHPREGAARLASEFS
HHHHHHHCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHC
NGTPVINAGDGANQHPTQTLLDLYTIYETQGRLDNLNIAFVGDLKYGRTVHSLTQALAKF
CCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
NGVKFFFIAPEVLAMPDYICEELDELGIEYQLLNSMEEAIPELDILYMTRVQKERFDESE
CCCEEEEECCHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHH
YAHIKSAYILSAADLKPARENLKVLHPLPRIDEINIDVDKTPHAYYFQQAENGVYARQAL
HHHHHHHHEEEHHCCCCCHHCHHHHCCCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHH
LALVLNESL
HHHHHHCCC
>Mature Secondary Structure 
ANSLYQKHIISIPELSREELELIVETAGNIKKEPQPDLLKNKVIASCFFEASTRTRLSF
CCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
ETAIQRLGGSVIGFDSAGNTSLAQKGETLADSVQIIASYANAYVMRHPREGAARLASEFS
HHHHHHHCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHC
NGTPVINAGDGANQHPTQTLLDLYTIYETQGRLDNLNIAFVGDLKYGRTVHSLTQALAKF
CCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
NGVKFFFIAPEVLAMPDYICEELDELGIEYQLLNSMEEAIPELDILYMTRVQKERFDESE
CCCEEEEECCHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHH
YAHIKSAYILSAADLKPARENLKVLHPLPRIDEINIDVDKTPHAYYFQQAENGVYARQAL
HHHHHHHHEEEHHCCCCCHHCHHHHCCCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHH
LALVLNESL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA